scholarly journals Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells

2021 ◽  
Vol 9 (12) ◽  
pp. 2173-2178
Author(s):  
Muhammet Mükerrem Kaya ◽  
Soner Tutun ◽  
Melike Sultan Usluer ◽  
Hidayet Tutun
Keyword(s):  
Antiproliferative Activity ◽  
Food Product ◽  
Acetic Acid Bacteria ◽  
Antiproliferative Effect ◽  
Anticancer Activities ◽  
Myeloma Cells ◽  
Antiproliferative Effects ◽  
Diphenyltetrazolium Bromide ◽  
Ancient Times ◽  
Cell Medium

Vinegar is an aqueous food product made by a succession of yeast and acetic acid bacteria activities from fruits that contain high carbohydrates such as apples and grapes. Vinegar has been used as a dietary spice and natural remedy since ancient times due to its therapeutic properties including antimicrobial, antidiabetic, and anticancer activities. It has been shown that some bioactive compounds exhibiting antioxidant activity in vinegars lead to anticancer activity. The aim of the present study was to investigate antiproliferative effect of commercial and home-made apple vinegars in native and neutralized form on myeloma cells. In order to neutralize the vinegars, sodium hydroxide (NaOH) was used. A serial two-fold dilutions of the vinegars (50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%) prepared with cell medium were treated to the cells. The MTT (3-(4.5-Dimethylthiazol-2-yl)-2.5-Diphenyltetrazolium Bromide) assay was used to determine the cellular viability in the cells treated with the vinegars. In this study, while commercial vinegar possessed a stronger antiproliferative activity than home-made vinegar, all native vinegars possessed stronger antiproliferative effect than neutralized vinegars. Interestingly, when home-made vinegar (both native and neutralized) concentrations were from 6.25 to 1.56%, the cell viability increased. Apple vinegar exhibited antiproliferative activity on myeloma cells; however, further studies are required to clarify the mechanisms underlying this activity.

Antiproliferative activity of lichen extracts on murine myeloma cells

Biologia ◽  
2009 ◽  
Vol 64 (1) ◽  
Author(s):  
Doriana Triggiani ◽  
Donatella Ceccarelli ◽  
Antonio Tiezzi ◽  
Tommaso Pisani ◽  
Silvana Munzi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Antiproliferative Activity ◽  
Myeloma Cell ◽  
Living Cells ◽  
Evernia Prunastri ◽  
Myeloma Cells ◽  
Methanolic Extracts ◽  
Mammalian Cell Lines ◽  
Diphenyltetrazolium Bromide ◽  
Xanthoria Parietina

AbstractIn the present study we report some preliminary results concerning the evaluation of antiproliferative activity on murine myeloma cells (P3X63-Ag8.653) of crude extracts of two common lichen species, Evernia prunastri and Xanthoria parietina.The results were evaluated by means of the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test, which is commonly used to assess the activity of living cells through mitochondrial dehydrogenases. They indicated that extracts of E. prunastri had no effect, while those of X. parietina significantly affected murine myeloma cell proliferation, with a reduction down to 75% for methanolic extracts. This opens perspectives for deeper investigations extended also to other mammalian cell lines.

Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid

2020 ◽  
Vol 6 (2) ◽  
pp. 134-146 ◽  
Author(s):  
Kehkashan Arshad Qamar ◽  
Ahsana Dar Farooq ◽  
Bina S. Siddiqui ◽  
Nurul Kabir ◽  
Sabira Begum
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Mitotic Spindle ◽  
Immunofluorescence Microscopy ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Ocimum Basilicum ◽  
Antiproliferative Effects ◽  
Ancient Times ◽  
Mcf 7

Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Objective: To emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy. Method: O. basilicum (aerial parts) methanolic extract and fractions were screened against HT-144, MCF-7, NCI-H460 and SF-268 human cancer cell lines using sulforhodamine B assay. The more active Petroleum Ether Insoluble (PEI) fraction was fractionated into six sub-fractions (OB-1 to OB-6). Four pure compounds (3-O-methyl ursolic acid, oleanolic acid, 3-epi-ursolic acid and ursolic acid) were isolated from the more potent sub-fraction OB- 6. Triple channel immunofluorescence microscopy was employed to observe the effects of methanolic extract, PEI fraction, sub-fractions OB-5 and OB-6, 3-epi-ursolic acid and oleanolic acid on the cytoskeleton and nuclei of MCF-7 cells. Result: The methanolic extract and the PEI fraction exhibited selectively greater growth inhibition against MCF-7 cell line (TGI: 56 and 36.2 µg/ml, respectively). By using triple channel immunofluorescence microscopy, it was observed that the methanolic extract, PEI fraction, sub-fraction OB-5 and 3-epi-ursolic acid induced irregular mitotic spindle formation and slowing of mitotic progression in MCF-7 cells while sub-fraction OB-6 induced mitotic arrest in the prophase stage. F-actin aggregation was also visible in PEI fraction, subfraction OB-5 and 3-epi-ursolic acid treated MCF-7 cells. Conclusion: These results emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy and suggest that it inhibits the growth of MCF-7 cancer cells via multiple mechanisms such as interaction with the microtubules and mitotic spindle apparatus, and F-actin aggregation.

scholarly journals Antiproliferative Effects of Alkaloid Evodiamine and Its Derivatives

2018 ◽  
Vol 19 (11) ◽  
pp. 3403 ◽  
Author(s):  
Xu Hu ◽  
Dahong Li ◽  
Chun Chu ◽  
Xu Li ◽  
Xianhua Wang ◽  
...  
Keyword(s):  
Systematic Review ◽  
Natural Products ◽  
Drug Administration ◽  
Antiproliferative Activity ◽  
Natural Source ◽  
Antiproliferative Effects ◽  
Anti Cancer ◽  
Ring Structures ◽  
First Time ◽  
Antiproliferative Activities

Alkaloids, a category of natural products with ring structures and nitrogen atoms, include most U.S. Food and Drug Administration approved plant derived anti-cancer agents. Evodiamine is an alkaloid with attractive multitargeting antiproliferative activity. Its high content in the natural source ensures its adequate supply on the market and guarantees further medicinal study. To the best of our knowledge, there is no systematic review about the antiproliferative effects of evodiamine derivatives. Therefore, in this article the review of the antiproliferative activities of evodiamine will be updated. More importantly, the antiproliferative activities of structurally modified new analogues of evodiamine will be summarized for the first time.

PKA/AMPK signaling in relation to adiponectin’s antiproliferative effect on multiple myeloma cells

Leukemia ◽  
2014 ◽  
Vol 28 (10) ◽  
pp. 2080-2089 ◽  
Author(s):  
E A Medina ◽  
K Oberheu ◽  
S R Polusani ◽  
V Ortega ◽  
G V N Velagaleti ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Antiproliferative Effect ◽  
Myeloma Cells ◽  
Ampk Signaling

scholarly journals Synthesis and Antiproliferative Activity of 2,4,6,7-Tetrasubstituted-2H-pyrazolo[4,3-c]pyridines

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6747
Author(s):  
Beatričė Razmienė ◽  
Eva Řezníčková ◽  
Vaida Dambrauskienė ◽  
Radek Ostruszka ◽  
Martin Kubala ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Nuclear Antigen ◽  
Ph Sensing ◽  
Caspase 9 ◽  
Ph Indicator ◽  
Antiproliferative Effects ◽  
Alkylation Reactions ◽  
Ratiometric Ph Sensing ◽  
Proliferating Cell ◽  
Parp 1

A library of 2,4,6,7-tetrasubstituted-2H-pyrazolo[4,3-c]pyridines was prepared from easily accessible 1-phenyl-3-(2-phenylethynyl)-1H-pyrazole-4-carbaldehyde via an iodine-mediated electrophilic cyclization of intermediate 4-(azidomethyl)-1-phenyl-3-(phenylethynyl)-1H-pyrazoles to 7-iodo-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridines followed by Suzuki cross-couplings with various boronic acids and alkylation reactions. The compounds were evaluated for their antiproliferative activity against K562, MV4-11, and MCF-7 cancer cell lines. The most potent compounds displayed low micromolar GI50 values. 4-(2,6-Diphenyl-2H-pyrazolo[4,3-c]pyridin-7-yl)phenol proved to be the most active, induced poly(ADP-ribose) polymerase 1 (PARP-1) cleavage, activated the initiator enzyme of apoptotic cascade caspase 9, induced a fragmentation of microtubule-associated protein 1-light chain 3 (LC3), and reduced the expression levels of proliferating cell nuclear antigen (PCNA). The obtained results suggest a complex action of 4-(2,6-diphenyl-2H-pyrazolo[4,3-c]pyridin-7-yl)phenol that combines antiproliferative effects with the induction of cell death. Moreover, investigations of the fluorescence properties of the final compounds revealed 7-(4-methoxyphenyl)-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridine as the most potent pH indicator that enables both fluorescence intensity-based and ratiometric pH sensing.

scholarly journals Metformin synergistically enhances antiproliferative effects of cisplatin and etoposide in NCI-H460 human lung cancer cells

2013 ◽  
Vol 39 (6) ◽  
pp. 644-649 ◽  
Author(s):  
Sarah Fernandes Teixeira ◽  
Isabella dos Santos Guimarães ◽  
Klesia Pirola Madeira ◽  
Renata Dalmaschio Daltoé ◽  
Ian Victor Silva ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Line ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Antiproliferative Effects ◽  
H460 Cell ◽  
Diphenyltetrazolium Bromide ◽  
Human Lung Cancer Cells ◽  
Liver Kinase B1 ◽  
Independent Effects

OBJECTIVE: To test the effectiveness of combining conventional antineoplastic drugs (cisplatin and etoposide) with metformin in the treatment of non-small cell lung cancer in the NCI-H460 cell line, in order to develop new therapeutic options with high efficacy and low toxicity.METHODS: We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and calculated the combination index for the drugs studied.RESULTS: We found that the use of metformin as monotherapy reduced the metabolic viability of the cell line studied. Combining metformin with cisplatin or etoposide produced a synergistic effect and was more effective than was the use of cisplatin or etoposide as monotherapy.CONCLUSIONS: Metformin, due to its independent effects on liver kinase B1, had antiproliferative effects on the NCI-H460 cell line. When metformin was combined with cisplatin or etoposide, the cell death rate was even higher.

scholarly journals Characterization and Antiproliferative Activity of Nobiletin-Loaded Chitosan Nanoparticles

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ana G. Luque-Alcaraz ◽  
Jaime Lizardi ◽  
Francisco M. Goycoolea ◽  
Miguel A. Valdez ◽  
Ana L. Acosta ◽  
...  
Keyword(s):  
Schiff Base ◽  
Carbonyl Group ◽  
Cancer Chemotherapy ◽  
Antiproliferative Activity ◽  
Chitosan Nanoparticles ◽  
Aqueous Solubility ◽  
Antiproliferative Effect ◽  
Amine Groups ◽  
Imine Bond ◽  
Cancerous Cells

Nobiletin is a polymethoxyflavonoid with a remarkable antiproliferative effect. In order to overcome its low aqueous solubility and chemical instability, the use of nanoparticles as carriers has been proposed. This study explores the possibility of binding nobiletin to chitosan nanoparticles, as well as to evaluate their antiproliferative activity. The association and loading efficiencies are 69.1% and 7.0%, respectively. The formation of an imine bond between chitosan amine groups and the carbonyl group of nobiletin, via Schiff-base, is proposed. Nobiletin-loaded chitosan nanoparticles exhibit considerable inhibition (IC50=8 μg/mL) of cancerous cells, revealing their great potential for applications in cancer chemotherapy.

scholarly journals Nitric Oxide Is a Mediator of Antiproliferative Effects Induced by Proinflammatory Cytokines on Pancreatic Beta Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Laura Quintana-Lopez ◽  
Manuel Blandino-Rosano ◽  
Gonzalo Perez-Arana ◽  
Alberto Cebada-Aleu ◽  
Alfonso Lechuga-Sancho ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Proinflammatory Cytokines ◽  
Pancreatic Beta Cells ◽  
Antiproliferative Effect ◽  
Bb Rat ◽  
Antiproliferative Effects ◽  
Initial Stage ◽  
Rat Strain ◽  
Nos Inhibitor

Nitric oxide (NO) is involved in several biological processes. In type 1 diabetes mellitus (T1DM), proinflammatory cytokines activate an inducible isoform of NOS (iNOS) inβcells, thus increasing NO levels and inducing apoptosis. The aim of the current study is to determine the role of NO (1) in the antiproliferative effect of proinflammatory cytokines IL-1β, IFN-γ, and TNF-αon cultured isletβcells and (2) during the insulitis stage prior to diabetes onset using the Biobreeding (BB) rat strain as T1DM model. Our results indicate that NO donors exert an antiproliferative effect onβcell obtained from cultured pancreatic islets, similar to that induced by proinflammatory cytokines. This cytokine-induced antiproliferative effect can be reversed by L-NMMA, a general NOS inhibitor, and is independent of guanylate cyclase pathway. Assays using NOS isoform specific inhibitors suggest that the NO implicated in the antiproliferative effect of proinflammatory cytokines is produced by inducible NOS, although not in an exclusive way. In BB rats, early treatment with L-NMMA improves the initial stage of insulitis. We conclude that NO is an important mediator of antiproliferative effect induced by proinflammatory cytokines on culturedβcell and is implicated inβ-cell proliferation impairment observed early from initial stage of insulitis.

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