Determination of the quantitative content of the amount of organic acids and ascorbic acid in different batches of Rosa majalis and Rosa canina raw material

Aim. To analyze the results of the quantitative content of the amount of organic acids in rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite by the potentiometric method, which is available in the monograph “Hibiscus” in the SPhU 2.0. Materials and methods. The study subjects were batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite. The quantitative content of the amount of organic acids was determined by the potentiometric method from the monograph “Hibiscus” SPhU 2.0 using a HI 2550 potentiometer of the “HANNA instruments” company (Germany). Results and discussion. The highest content of the amount of organic acids, among the study subjects was in roots of Arctium minus (from 1.02 ± 0.04 % to 1.05 ± 0.04 %). At the same time, in roots of Arctium tomentosum this amount was almost 3 times lower (from 0.36 ± 0.01 % to 0.37 ± 0.01 %). In the batches of roots of Rosa majalis the content of this group of compounds was almost 2 times higher than in roots of Rosa canina. In rhizomes with roots of Rumex confertus and rhizomes with roots of Sanguisorba officinalis the content of the amount of organic acids differed insignificantly. In herb of Bidens tripartite the content of this group of compounds was 0.78 ± 0.03 – 0.79 ± 0.03 %. In different batches of one type of the raw material the quantitative content of the amount of organic acids varied slightly. Conclusions. For the first time, the quantitative content of the amount of organic acids has been determined in the batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite using the potentiometric method which is available in the monograph “Hibiscus” in the SPhU 2.0. It has been found that the highest content of the amount of organic acids is observed in roots of Arctium minus (not less than 1.02 %) and herb of Bidens tripartite (not less than 0.78 %). The results obtained will be used in further research when creating new herbal medicines with the anti-inflammatory and antimicrobial activity.

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Identification and determination of ascorbic acid, free organic acids and tannic substances in the grass of Inula L. genus species

French-Ukrainian Journal of Chemistry ◽

10.17721/fujcv7i1p25-33 ◽

2019 ◽

Vol 7 (1) ◽

pp. 25-33

Author(s):

Olena Yerenko ◽

Galina Smoylovska ◽

Taya Khortetska

Keyword(s):

Ascorbic Acid ◽

Organic Acids ◽

Quantitative Determination ◽

Biologically Active ◽

Raw Material ◽

Grass Species ◽

Inula Britannica ◽

Inula Helenium ◽

Inula Britannica L

The level of studying of Inula L. genus species, growing on the territory of Ukraine, is insufficient today. The study of chemical composition, accumulation of biologically active substances, pharmacological action of investigated grass species’ extracts of Inula L. genus of Ukrainian flora are of current interest. The aim of the work is making qualitative and comparative analysis of ascorbic, organic acids and tannic substances content in the raw material of investigated species of Inula L. genus (Inula britannica L. and Inula helenium L.). Quantitative determination was carried out by volume titration method. The presence of ascorbic acid, organic acids and tannic substances in the raw material of Inula L. genus investigated species was established with the help of paper chromatography and thin-layer chromatography methods. The quantitative determination of total organic acids was carried out according to XI edition of State Pharmacopoeia methods in terms of malic acid. Water extracts were studied for the tannins presence in raw material of Inula L. genus investigated species. Compounds identification was carried out through chemical reactions in accordance with Ukrainian State Pharmacopeia. The results obtained showed the perspective of further study of the biological activity of Inula britannica L. and Inula helenium L.

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Comparative pharmakognostical study of roots of Rosa Majalis Herrm. and Rosa Canina L.

ScienceRise Pharmaceutical Science ◽

10.15587/2519-4852.2021.243525 ◽

2021 ◽

pp. 71-78

Author(s):

Tetiana Oproshanska ◽

Olga Khvorost ◽

Kateryna Skrebtsova ◽

Konradas Vitkevicius

Keyword(s):

Cell Walls ◽

Raw Materials ◽

Total Polyphenols ◽

Diagnostic Features ◽

Rosa Canina ◽

Plant Raw Materials ◽

Quantitative Content ◽

Microscopic Features ◽

Boundary Limits

The aim is to conduct a comparative pharmacognostical study of the series of roots of Rosa (R.) majalis Herrm. and Rosa (R.) canina L. with the establishment of diagnostic features of morphological and anatomical structure and boundary limits of numerical indicators of raw materials. Materials and methods. The fresh and dry raw materials were used to study the macroscopic microscopic features by microscope Delta optical BioLight 300 (Poland). Determination of total polyphenols was performed by spectrophotometry (on a spectrophotometer Optizen POP (Korea)) and HPLC (chromatograph an Agilent 1200 3 D LC System Technologies (USA)). Results. The morphological (nature of the surface (periderm) and fracture) and anatomical (color of cell walls and their cavities; location of the sclerenchyma; the presence of a crystalline coating of the sclerenchyma at the root of R. canina; of various elements of the remains of the tetraarchic conducting bundle in the center of the root) diagnostical features of roots of R. majalis and R. Canina were established. Comparing the numerical values of loss on drying (not more than 10 %), total ash (not more than 5 %), extractable matter (not less than 9 %) and the quantitative content of total polyphenols (not less than 4 %) it was determined that both types of raw materials according to these indicators are almost indistinguishable. Conclusions. Loss on drying, total ash, extractable matter and content of total polyphenols of the root of R. majalis and R. canina do not have significant differences, that is why the root of both plant species can be used as medicinal plant raw materials such as “Rose root”. The obtained data will be used in further research when creating methods of quality control of plant raw materials and phytomedicines

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Extraction and quantitative determination of ascorbic acid during different maturity stages of Rosa canina L. fruit

Journal of Food Composition and Analysis ◽

10.1016/j.jfca.2007.11.007 ◽

2008 ◽

Vol 21 (4) ◽

pp. 300-305 ◽

Cited By ~ 55

Author(s):

Saeed Nojavan ◽

Faezeh Khalilian ◽

Fatemeh Momen Kiaie ◽

Atyeh Rahimi ◽

Armin Arabanian ◽

...

Keyword(s):

Ascorbic Acid ◽

Quantitative Determination ◽

Rosa Canina ◽

Maturity Stages

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HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli

Pharmacia ◽

10.3897/pharmacia.66.e35194 ◽

2019 ◽

Vol 66 (4) ◽

pp. 161-164

Author(s):

O. A. Kyslychenko ◽

Viktoriia V. Protska ◽

Iryna O. Zhuravel

Keyword(s):

Phenolic Compounds ◽

Inflammatory Diseases ◽

Raw Materials ◽

Folk Medicine ◽

Usnic Acid ◽

Total Content ◽

Raw Material ◽

Parmelia Sulcata ◽

Quantitative Content

The species of Parmelia genus have long been used in Indian folk medicine for the treatment of bronchitis, ulcers, furunculosis, cardiovascular diseases, urolithiasis, amenorrhea, and also at infectious and inflammatory diseases. In Ukraine, the most common lichens of the Parmelia genus are Parmelia sulcata Tailor and Parmelia vagans Nyl. At the same time, thalli of Parmelia genus lichens belong to the non-officinal and poorly studied types of raw material. The qualitative composition and the quantitative content of phenolic compounds in Parmelia sulcata and Parmelia vagans thalli was studied by HPLC. According to the results of the chromatographic analysis, salazinic, fumaroprotocetraric, usnic acids, chloratranorin and atranorin were identified in both types of raw material studied. In addition, protocetraric acid was identified in Parmelia sulcata thalli. According to the results of the experiment, the total content of identified phenolic compounds in Parmelia sulcata thalli was 2019.71±40.39 g/mol, and in Parmelia vagans thalli it comprised 1754.18±34.77 g/mol. In the thalli of both studied species of Parmelia genus, fumaroprotocetraric acid dominanted by the quantity. This substance was present in Parmelia sulcata thalli in the amount of 474.00±9.00 g/mol, and in Parmelia vagans thalli – 456.21±8.67 g/mol. In addition, a significant amount of chloratranorin (408.79±8.99 g/mol) was present in Parmelia sulcata thalli. Quite a high content of atranorin (393.34±8.65 g/mol) and usnic acid (375.31±7.53 g/mol) were defined in Parmelia vagans thalli. The results obtained can be used in the development of quality control methods for Parmelia sulcata and Parmelia vagans thalli, as well as medicines based on these types of raw materials.

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NUTRITIONAL AND BIOLOGICAL VALUE OF FRUITS OF CRATAEGUS OXYACANTHA L.

chemistry of plant raw material ◽

10.14258/jcprm.2020015452 ◽

2020 ◽

pp. 265-275

Author(s):

Lyubov' Nikolayevna Skrypnik ◽

Irina Petrovna Melnichuk ◽

Yulia Vladimirovna Koroleva

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Phenolic Compounds ◽

Organic Acids ◽

Food Additives ◽

Raw Material ◽

Transport Infrastructure ◽

Anthropogenic Factors ◽

Total Phenolic ◽

Phytochemical Study

The work involved the phytochemical study of hawthorn fruits (Crataegus oxyacantha L.). Hawthorn fruits were collected in areas with low anthropogenic impact (landscape and recreational areas) and in areas with the transport infrastructure in Kaliningrad city. The content of total phenolic compounds and subgroups: catechins, leucoanthocyanins, flavonoids, tannins); monosaccharides (glucose and fructose), pectins, organic acids, ascorbic acid, macro- and microelements, as well as antioxidant activity in hawthorn fruits were studied. The influence of the anthropogenic factor on the variability of their chemical composition was shown. It has been established that hawthorn fruits from areas with minimal impact of anthropogenic factors can accumulate phenolic compounds (up to 15.9 mg/g), leucoanthocyanins (up to 1.5 mg/g), tannins (up to 6.5 mg/g ), catechins (up to 4.1 mg/g), flavonoids (up to 6.0 mg/g), fructose (up to 14.1%), pectin (up to 11.6%), organic acids (1.45%), ascorbic acid (up to 49.3 mg/100 g), calcium (up to 12.12 mg/g), zinc (up to 39.12 mg/kg) more intensively and demonstrated higher antioxidant activity (up to 9.7 mg/g). The cluster analysis carried out on the content of phytochemicals proved the dependence of the accumulation of nutrients in hawthorn fruits on the growing conditions of plants. The obtained results allow us to consider the fruits of hawthorn C. oxyacantha as a valuable raw material for use as food additives for various purposes.

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Research in the Development and Validation of the Method of Quantitative Determination of Flavonoids in the Common Sorrel Herba, Gathered in the Altai Territory

Drug development & registration ◽

10.33380/2305-2066-2019-8-2-80-86 ◽

2019 ◽

Vol 8 (2) ◽

pp. 80-86 ◽

Cited By ~ 1

Author(s):

G. R. Kutateladze ◽

L. M. Fedoseeva

Keyword(s):

Particle Size ◽

Aluminum Chloride ◽

Raw Materials ◽

Alcohol Solution ◽

Raw Material ◽

Complexation Reaction ◽

Optimal Parameters ◽

Quantitative Content ◽

Development And Validation

Introduction. Common sorrel (Rumex acetosa L.) is a biennial herb from the Buckwheat family (Polygonaceae L.). In our previously studies of phenolic compounds composition of alcohol extraction and ethereal, ethyl acetate, butanol fractions of common sorrel herba and flavonoids of the flavonol group was found. From the literature sources it is known that flavonoids are one of the groups of biologically active compounds with anti-inflammatory action. In this regard, the actual task is to assess the quantitative content of flavonoids in common sorrel herba.Aim. Is the development and validation of a method for the quantitative determination of flavonoids in common sorrel herba.Materials and methods. It has been established that the flavonoid complex of common sorrel herba includes rutin, therefore, the reference sample (RS) rutin was used as a standard for calculating the amount of flavonoids. Alcohol extract of common sorrel herba and a solution of rutin RS were prepared. The complexation reaction with aluminum chloride was carried out. The resulting solutions were investigated by the method of differential spectrophotometry. The spectral characteristics of the test and standard samples were compared. Then we studied the effect of extraction conditions on the yield of flavonoids from the raw material: the extractant, the particle size of the raw material, the ratio of «raw material – extractant», temperature, frequency and duration of extraction. Purified water and ethyl alcohol of various concentrations (20%, 40%, 70%, 90%) were used as the extractant. Next, carried out the selection of the optimal conditions for the complexation reaction (the complexation reaction time, the ratio of «aliquot – aluminum chloride alcohol solution»). The method was validated according to GPM.1.1.0012.15 of the State Pharmacopoeia (SF) XIII edition and generally accepted methods for the following indicators: specificity, analytical field, linearity, accuracy, precision.Results and discussion. The optimal parameters for extracting flavonoids from raw materials were determined (threefold extraction with ethyl alcohol 70% in a water bath, the ratio of «raw material – extractant» – 1:30 for 30 minutes, the particle size of the raw material – 2.0 mm). The conditions for the complexation reaction were selected (the ratio «aliquot: aluminum chloride alcohol solution» – 1: 2.5, the complexing agent – aluminum chloride solution 5% alcohol, the appearance of a stable solution color after 40 minutes). When carrying out the validation of the developed method, it was established that the validation characteristics under study are within the acceptance criteria. When analyzing the raw materials harvested in the Altai Territory in different years, it was found that the content of flavonoids in common sorrel herba ranges from 0,596 to 0,632%.Conclusion. The optimal parameters of extraction of flavonoids from raw materials were determined, the conditions for the complexation reaction were selected, and the developed method was validated. The quantitative content of flavonoids in terms of rutin in sorrel sour grass harvested in the Altai Territory in different years has been established.

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THE STUDY OF QUALITATIVE COMPOSITION AND DETERMINATION OF THE QUANTITATIVE CONTENT OF FLAVONOIDS IN RAW MATERIAL OF HELIOPHYSIS HELIANTHOIDES

Fitoterapia ◽

10.33617/2522-9680-2019-2-41 ◽

2019 ◽

Vol 2 (2) ◽

pp. 41-44

Author(s):

M. Yu. Pavlenko-Badnaoui ◽

◽

V. V. Protska ◽

I. O. Zhuravel ◽

I. G. Gurieva ◽

...

Keyword(s):

Raw Material ◽

Qualitative Composition ◽

Quantitative Content

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СРАВНИТЕЛЬНЫЙ АНАЛИЗ МЕТОДИК КОЛИЧЕСТВЕННОГО ОПРЕДЕЛЕНИЯ АСКОРБИНОВОЙ КИСЛОТЫ В СУБСТАНЦИИ

INTERNATIONAL ACADEMY JOURNAL Web of Scholar ◽

10.31435/rsglobal_wos/30112019/6806 ◽

2019 ◽

Vol 1 (11(41)) ◽

pp. 32-36

Author(s):

Д. Т. Гаибназарова ◽

Г. У. Тиллаева ◽

Д. Б. Касимова ◽

У. М. Тиллаева

Keyword(s):

Ascorbic Acid ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Comparative Analysis ◽

Quantitative Determination ◽

High Performance ◽

Quantitative Content ◽

Physicochemical Methods

Increasing requirements for the safety, effectiveness and quality of medicines make it necessary to develop new and improve existing methods for their analysis. As a rule, the quality of substances is ensured by a set of analytical methods that confirm their authenticity, determine the purity and quantitative content of the active substance.In this study, a comparative analysis of the physicochemical methods for the quantitative determination of ascorbic acid in substances is carried out. The advantage of using the method of high performance liquid chromatography over the spectrophotometric method is determined.

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DETERMINATION OF CARBOHYDRATES CONTENT IN GENTIANA CRUCIATA L. BY GC/MS METHOD

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021v13i1.39820 ◽

2021 ◽

pp. 124-128

Author(s):

LILIIA BUDNIAK ◽

LIUDMYLA SLOBODIANIUK ◽

SVITLANA MARCHYSHYN ◽

PAVLINA KLEPACH ◽

YANA HONCHARUK

Keyword(s):

Plant Material ◽

Raw Material ◽

Qualitative Composition ◽

Mass Spectral Library ◽

Spectral Library ◽

Mass Spectral ◽

Quantitative Content ◽

Free Carbohydrates ◽

Gentiana Cruciata

Objective: Thus, the aim of our research was to determine the qualitative composition and quantitative content of carbohydrates in the studied plant material with the prospect of its application as a medicinal plant raw material. Methods: The carbohydrates of the herb of Gentiana cruciata L. determined by GC/MS method. Identification of monosaccharides was based on comparing their retention times with retention times of standards of the mass spectral library NIST 02. Quantification was done by using sorbitol added to the sample. Results: The quantitative content of 4 free carbohydrates such as D-saccharose (38.39 mg/g), D-Pinitol (12.01 mg/g), D-glucose (10.05 mg/g) and D-fructose (1.69 mg/g) was established in the herb of Gentiana cruciata L. Also, this method established the qualitative composition and quantitative content of eight carbohydrates (monosaccharides and their derivatives after hydrolysis): D-glucose (29.66 mg/g), D-Pinitol (22.24 mg/g), L-arabinose (4.26 mg/g), D-galactose (3.55 mg/g), D-xylose (1.80 mg/g), L-rhamnose (1.49 mg/g), D-Dulcitol (0.76 mg/g) and D-mannose (0.44 mg/g). Conclusion: The results of the study showed that carbohydrates from the Gentiana cruciata L. can be used as important resources of new ingredients for the pharmaceutical industry.

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