Histopathological effects of fibrin glue on penile fracture in a rat model

Purpose: To evaluate both histopathological effects and potential clinical application of fibrin glue on the penile cavernosal tissue. Methods: Experimental penile fracture was formed by incising from the proximal dorsal side of the penis in 32 Wistar Albino rats. The rats were randomly assigned to four main groups of eight animals each. In the control group, the incision was not repaired and it was left to secondary healing. In the primary repair group, the incision was primarily repaired. In the fibrin glue group, glue was applied only to the incision. In the final group, fibrin glue was applied to the incision following primary repair. Three weeks later, penectomy tissue was examined histopathologically. Results: When the control group was compared with primary repair+fibrin glue group, the differences in cavernous tissue healing with fibrosis and inflammation were statistically significant (p = 0.04 and 0.01, respectively). The primary repair+fibrin glue group, showed the best cavernous healing with fibrosis observed in only one rat. . There was no significant difference between the control group and the other groups according to cavernous tissue healing with fibrosis and inflammation (p = 0.11 and 0.12). Hyperemia was observed in the all groups of rats. Conclusions: Fibrin glue can be used in cavernoseal surgeries due to its adhesive and potentially anti-inflammatory features.

Download Full-text

The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽

10.3390/molecules26051332 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1332

Author(s):

Gilda M. Iova ◽

Horia Calniceanu ◽

Adelina Popa ◽

Camelia A. Szuhanek ◽

Olivia Marcu ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Diabetic Rats ◽

Gingival Tissue ◽

Control Group ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Significant Difference ◽

The Impact ◽

Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

Download Full-text

Histological Study On Using Ileal Submucosa In Repairing Urinary Bladder Defect In Adult Albino Rat

QJM ◽

10.1093/qjmed/hcab099.004 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Eman Gomaa El Saeed ◽

Manal H Moussa ◽

Gehad A Hammouda ◽

Sahar M. M Omar

Keyword(s):

Urinary Bladder ◽

Histological Study ◽

Squamous Metaplasia ◽

Muscle Layer ◽

Control Group ◽

Albino Rats ◽

Adult Rats ◽

Group I ◽

Significant Difference ◽

Graft Area

Abstract Background Repairing urinary bladder (UB) defect by enterocystoplasty remains the gold standard surgical bladder reconstruction procedure to increase the capacity and compliance of dysfunctional bladders. However, many complications were recorded. Aim of the work This work aimed to compare the consequences of reconstruction of urinary bladder defect using untreated small intestinal submucosal (SIS) matrix versus seeded and unseeded decellularized SIS matrix. Material and Methods Fifty female albino rats were used in this study. The animals were divided into three groups: Group I (Control) included ten adult rats from which ileal tissue was obtained. Group II included ten adult rats in which their UB defect was repaired by untreated cellular SIS. Group III included twenty adult rats that were subdivided into two subgroups, 10 rats each; Subgroup IIIA where rats had their UB defect repaired by acellular SIS and subgroup IIIb where rats had their UB defect repaired by acellular SIS seeded with adipose mesenchymal stem cells (AMSCs).Ten young rats were used for preparation of AMSCs. Morphometric and statistical analysis were also performed. Results In rats where UB defect was repaired by untreated cellular SIS, the graft area showed loss of epithelial polarity, presence of intraepithelial cysts and occasional extension of urothelium to the outer surface forming fistula. There were areas of metaplasia with the appearance PAS positive cells. In the lamina propria, there was areas of lymphocytic infiltration together with significant increase in the collagen fiber deposition (p < 0.05). There was a significant decrease thickness of muscle layer as compared to control (p < 0.05). In rats where UB defect was repaired by acellular SIS, urothelium in the graft area showed occasional squamous metaplasia and often the urothelium extended to the deeper layers forming Brunn's nest. There was minimal muscle regeneration in the graft area. However, in rats where UB defect was repaired by acellular SIS seeded with AMSCs, the urothelium in the graft area was nearly similar to control group with uniform urothelium thickness, minimal collagen fibers deposition and thick muscle layer that showed no significant difference from the control (p > 0.05). Conclusion Acellular SIS seeded with AMSCs showed better results compared to non-seeded and cellular SIS in reconstructing urinary bladder defects.

Download Full-text

Effect of Tramadol on Sperm Profile of Male Albino Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2018/v3i429844 ◽

2019 ◽

pp. 1-6

Author(s):

I. S. Esua ◽

U. U. Uno ◽

U. B. Ekaluo

Keyword(s):

Sperm Motility ◽

Sperm Count ◽

Sperm Head ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Dependent Manner ◽

Sperm Viability ◽

Albino Rat ◽

Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

Download Full-text

Primary Repair for Injury of Medial Collateral Ligament During Total Knee Arthroplasty

Orthopaedic Journal of Sports Medicine ◽

10.1177/2325967120s00051 ◽

2020 ◽

Vol 8 (5_suppl5) ◽

pp. 2325967120S0005

Author(s):

Cheng Jin ◽

Jia-yi Zhao ◽

Jong-Keun Seon ◽

Asep Santoso

Keyword(s):

Clinical Outcomes ◽

Suture Anchor ◽

Primary Repair ◽

Hospital Length Of Stay ◽

Functional Score ◽

Control Group ◽

Subjective Feeling ◽

Valgus Stress ◽

Ambulatory Ability ◽

Significant Difference

This study, we aim to determine whether intraoperative over-release of MCL that is treated with primary repair can achieve satisfactory clinical results when compared to those who did not have over-release of MCL. At the same time, we seek to look into the difference between two methods of primary repair (anchor suture and staple) in terms of clinical outcomes. Purposes: We determined whether, after TKA, patients with CIA versus patients with CACB demonstrated (1) decreased pain scores (2) greater ambulatory ability postoperatively (3) decreased daily opioid consumption and hospital length of stay Methods: 3897 TKAs were performed from year 2003-2014. Sixty-five patients with MCL injury due to over-release during TKA who were repaired with suture anchor or staple (suture anchor: 36 vs. staple: 29) were studied whereas matched group of 65 patients without MCL injury were selected and served as the control group. Subjective feeling of instability and functional score were assessed using Knee Society Score (KSS) and Western Ontario & McMaster Universities Osteoarthritis Index (WOMAC). Objective stability was evaluated through measurement of opening angles in extension and 30° of knee flexion on valgus stress radiographs. The clinical and stability results of repair between the suture anchor and staple were compared. Results: The KSS and WOMAC scores in patients with primary repair of MCL during TKA from 50.6±14.8 to 87.3±8.3 and 65.9±16.3 to 17.7±8.3, respectively. However, there were neither statistical nor clinical significant difference when comparing between the group with primary repair and the control group. Radiographic stability also showed no differences between repair and control groups in extension and 30° of flexion (p= .63 and p= .37). Regarding the subgroups, There were no significant differences between the suture anchor and staple in terms of the stability and clinical outcomes Conclusion: Primary repair either with suture anchor or with staple for injury of the MCL in varus gonarthrosis during TKA provides a good stability and clinical outcomes as those of result with no MCL injury.

Download Full-text

The Effects of Hyperbaric Oxygen Therapy on Experimental Colon Anastomosis After Preoperative Chemoradiotherapy

International Surgery ◽

10.9738/cc130.2 ◽

2013 ◽

Vol 98 (1) ◽

pp. 33-42 ◽

Cited By ~ 7

Author(s):

Ramazan Yildiz ◽

Mehmet Fatih Can ◽

Gokhan Yagci ◽

Taner Ozgurtas ◽

Metin Guden ◽

...

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Preoperative Chemoradiotherapy ◽

Colon Resection ◽

Burst Pressure ◽

Control Group ◽

Albino Rats ◽

Colon Anastomosis ◽

Significant Difference

Abstract The aim of the present study was to investigate the effect of hyperbaric oxygen therapy (HBOT) on colon anastomosis after chemoradiotherapy (CRT). Sixty female Wistar-Albino rats were divided into 5 groups and underwent left colon resection and end-to-end anastomosis. CRT simulation was performed on 2 sham groups before the anastomosis, and 1 of these groups was administered additional postoperative HBOT. Two groups were administered CRT before the anastomosis, and 1 of them received additional postoperative HBOT. On postoperative day 5, all groups underwent relaparotomy; burst pressure was measured and samples were obtained for histopathologic and biochemical analysis. There was a significant weight loss in the CRT groups and postoperative HBOT had an improving effect. Significantly decreased burst pressure values increased up to the levels of the controls after HBOT. Hydroxyproline levels were elevated in all groups compared to the control group. Hydroxyproline levels decreased with HBOT after CRT. No significant difference was observed between the groups regarding fibrosis formation at the anastomosis site. However, regression was observed in fibrosis in the group receiving HBOT after CRT. Preoperative CRT affected anastomosis and wound healing unfavorably. These unfavorable effects were alleviated by postoperative HBOT. HBOT improved the mechanical and biochemical parameters of colon anastomosis in rats.

Download Full-text

Protective effect of N-acetylcysteine, caffeic acid and vitamin E on doxorubicin hepatotoxicity

Human & Experimental Toxicology ◽

10.1177/0960327107076885 ◽

2007 ◽

Vol 26 (6) ◽

pp. 519-525 ◽

Cited By ~ 36

Author(s):

A. Gokcimen ◽

A. Cim ◽

H.T. Tola ◽

D. Bayram ◽

A. Kocak ◽

...

Keyword(s):

Vitamin E ◽

Caffeic Acid ◽

Control Group ◽

Albino Rats ◽

Central Vein ◽

Protective Effects ◽

Injection Group ◽

Hepatocellular Damage ◽

Group I ◽

Significant Difference

The aim of this study was to compare the possible protective effects of N-acetylcysteine (NAC), caffeic acid (CAPE) and vitamin E (Vit-E) on doxorubicin-induced hepatotoxicity. Thirty-two male Wistar albino rats, weighing between 250 and 350 g were supplied and randomly divided into five groups. Animals in study groups were pretreated with a single dose of doxorubicin (Dox), which was administered intraperitoneally (i.p.). Control group (Group I) was treated with intraperitoneal saline injection. Group II did not received any antioxidant agent after the injection. Group III and Group IV were given CAPE and intraperitoneal vitamin E injection for eight days, respectively. Group V received NAC for eight days. The study was finished after 10 days. Tissue samples were collected from all animals and histopathological examination was performed. There was statistically significant difference between the experiment groups and controls by means of mononuclear cell infiltration and diameters of hepatic sinusoid, terminal hepatic venule (central vein) and portal area (portal canal). Changes related with hepatocellular damage were more prominent, whereas there was no significant difference between Dox and NAC given groups histopathologically. It was observed that structural changes were regressed after CAPE administration. However, this recovery was more prominent in vitamin E given group. These findings suggest that Dox induced liver damage could be efficiently reversed by vitamin E administration. It has been found that CAPE, but not NAC has protective effects on Dox-induced hepatocellular damage. Human & Experimental Toxicology (2007) 26, 519—525

Download Full-text

Evaluation of Anti-oxidant Enzymes, Lipid Peroxidation, Lipid Profile and Liver Function in Albino Rats Orally Administered Tartrazine

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i530188 ◽

2020 ◽

pp. 19-29

Author(s):

Uyota Anthony Adele ◽

Geraldine Iroh ◽

Ojoye Ngoye Briggs ◽

Helen Anthony Waribo ◽

Ibioku Elekima

Keyword(s):

Lipid Peroxidation ◽

Liver Function ◽

Lipid Profile ◽

Whole Blood ◽

Test Group ◽

Control Group ◽

Albino Rats ◽

Central Vein ◽

Significant Difference ◽

Anti Oxidant

Aim: To evaluate the anti-oxidant enzymes, lipid peroxidation, lipid profile and liver function in albino rats orally administered tartrazine. Study Design: A total number of 63 female albino rats weighing approximately 0.2 kg were used for this study. The study was divided into two phases, phase 1 which lasted for the first 30 days, comprised of 35 rats, 20 rats were used as test group while 15 rats served as the control group. Phase 2 of the study was for 60 days and 28 rats were used with 16 as test group and 12 as the control. The test groups were orally administered with 7.5 mg/kg of tartrazine (ADI) daily over the specified periods while the control groups were not treated with tartrazine but given only food and water. Place and Duration of Study: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria within a period of 12 months (Feb., 2019 – Jan., 2020). Methodology: At the end of the study, 5 mls of whole blood specimens was collected by means of cardiac puncture into plain bottles. To obtain the serum, the whole blood samples were allowed to clot and later dislodged and spun at 3500 rpm for 10 minutes. The collected serum specimens were used to analyze SOD, MDA, GPX, ALT, GGT, ALP, TG, TCHOL, and HDL-C, while LDL-C was calculated using Friedwald equation. Results: The chronic treatment of rats with tartrazine azo food dye at the ADI dose caused an increase in MDA levels after 30 and 60 days test rats compared to the control, while TCHOL and HDL-C showed significant decrease after 30 and 60 days of treatment in the test group compared to the control group. In addition, ALT indicated significant increase in test group after 60 days of treatment compared to control group. ALP, GGT, TG, LDL-C, SOD and GPX showed no significant difference after 30, and 60 days of treatment at ADI doses. Histologic examination of the liver indicated hydropic dilation, degenerating hepatocyes and infiltration of central vein with parenchymal materials alongside kupffer cells. Conclusion: The results from this study revealed that orally administered tartrazine at the recommended ADI dose increased lipid peroxidation as seen in the elevated MDA levels. Hepatic derangements were also seen as revealed by increased ALT and histologic distortions as well fall in TCHOL and HDL-C lipid fractions.

Download Full-text

Evaluation of the comparison of anti-depressant effects of oral fluoxetine and riluzole in albino rats by using the forced swimming test model

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20211021 ◽

2021 ◽

Vol 10 (4) ◽

pp. 391

Author(s):

Hansraj Kumar ◽

Akash Chandra ◽

Uma Shankar Prasad Keshri ◽

Rajiv Kumar

Keyword(s):

Selective Serotonin Reuptake Inhibitors ◽

Forced Swimming Test ◽

Antidepressant Activity ◽

Forced Swimming ◽

Control Group ◽

Albino Rats ◽

Test Duration ◽

Test Model ◽

Significant Difference ◽

Swimming Test

Background: Depression is a group of disorders results from a combination of multiple etiologic factors- genetic, biochemical, psychodynamic and socio-environmental. A depression consists of following clinical features as sadness, apathy, changes in sleep pattern, impaired concentration, feeling of shame or guilt and thoughts of dying or death. Fluoxetine and riluzole both are used for the treatment of depression in human being. Fluoxetine is SSRI (selective serotonin reuptake inhibitors) and riluzole is anxiolytic and mood stabilizer.Methods: Healthy male albino rats weighing between 150-200 grams were taken for the present study. Study animals were divided into three groups randomly with each group consisting of ten animals. Drugs were powdered with help of mortar and pestle and mixed in gum acacia solution. Appropriate volume of the freshly prepared solution was administered orally daily between 9 am to 10 am to all animal as per their individual body weight. Group A administered 1ml of 0.9% normal saline orally and serves as control group. Group B administered 0.4 mg of fluoxetine orally. Group C administered 2 mg of riluzole orally. Animals were evaluated for antidepressant activity using model- forced swimming test.Results: The results in the forced swimming test were assessed by duration of immobility in last 4 minutes of total 6 minute test duration. Antidepressant activity is indicated by the reduction in the duration of immobility i.e. lesser the duration more the efficacy. The results have been expressed as mean±standard deviation of duration of immobility in seconds during 6 minute period.Conclusions: There was significant difference in antidepressant activity of fluoxetine with antidepressant activity of riluzole. Riluzole showed antidepressant activity after two weeks of starting the drugs.

Download Full-text

The effect of antioxidants against electromagnetic field on enamel microhardness in rats

International Dental Research ◽

10.5577/intdentres.2019.vol9.no3.7 ◽

2019 ◽

Vol 9 (3) ◽

pp. 133-137

Author(s):

Ahmet Aras ◽

Mehmet Sinan Doğan ◽

Mehmet Cihan Yavaş

Keyword(s):

Electromagnetic Field ◽

Field Intensity ◽

Hardness Test ◽

Control Group ◽

Albino Rats ◽

Homogeneity Test ◽

Micro Hardness ◽

Dental Tissues ◽

Antioxidant Agents ◽

Significant Difference

Aim: In current studies, it is reported that exposure to electromagnetic field (EMF) is related to numerous illnesses. Important parts of our bodies, oral and dental tissues can also be influenced by the EMF. In this study, it was aimed to determine possible negative impacts of EMFs on dental enamel hardness and to test the efficiency of the antioxidant agents against possible adverse effects. Methodology: In this study, 4-month-old 32 Winster strain Albino rats were used, each weighing 250-300 gr in average. In total, 40 teeth were used from these 32 rats. These teeth were classified into four groups. Group 1 was determined as the control group, and Group 2,3, and 4 were exposed to EMF measured as 2.48 μT magnetic field intensity and 80.3 V / m electric field intensity for 52 days. Ganodermalucidum (20 mg/kg/ day) was applied to the Group 3 together with the EMF. Melatonin (10 mg/kg/ day) was applied to the Group 4 together with the EMF. The teeth pulled out after the euthanasia were embedded into acrylic blocks and micro-hardness test was applied. The normality of the variables was analyzed through the Shapiro-Wilk test. After the homogeneity test, one-way analysis of variance (ANOVA) was used to compare surface micro-hardness (p<0.05). Results: As a result of the conducted statistical analyses, there was statistically no significant difference among the groups concerning micro-hardness (P>0.05). Conclusions: It was determined in our study that 52-day exposure to 2.48 μT EMF did not cause any micro-hardness change in the tooth enamels of the rats. It was also observed that the used antioxidant agents did not cause any significant difference. How to cite this article: Aras A, Doğan MS, Yavaş MC. The effect of antioxidants against electromagnetic field on enamel microhardness in rats. Int Dent Res 2019;9(3):133-7. Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Download Full-text

Autologous omentum transposition for regeneration of a renal injury model in rats

Military Medical Research ◽

10.1186/s40779-021-00361-0 ◽

2022 ◽

Vol 9 (1) ◽

Author(s):

Tayfun Bilgiç ◽

Ümit İnce ◽

Fehmi Narter

Keyword(s):

Foreign Body ◽

Foreign Body Reaction ◽

Primary Repair ◽

Healing Process ◽

Kidney Injury ◽

Control Group ◽

Albino Rats ◽

Loss Of Function ◽

Omentum Transposition ◽

Process Completion

Abstract Background After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model. Methods A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively, and their right kidneys were taken for histopathological evaluation. Results The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (P = 0.005 and P = 0.004, respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P > 0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P > 0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (r = 0.824, P < 0.001) and foreign body reaction in the kidney specimen (r = 0.872, P < 0.001) and a strong and negative correlation with the healing process completion score in the kidney (r = − 0.627, P = 0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (r = 0.731, P = 0.001) and strongly and negatively correlated with the healing process completion score in the kidney specimen (r = − 0.608, P = 0.002). Conclusion Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally, the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss.

Download Full-text