scholarly journals The Difference in Average of Maternal Serum Hypoxia-Inducible Factors-1α Levels between Early Onset and Late-Onset Severe Preeclampsia

2021 ◽  
Vol 5 (1) ◽  
pp. 82-89
Author(s):  
David Perdana ◽  
Defrin Defrin ◽  
Firdawati Firdawati
Keyword(s):  
Early Onset ◽  
Sampling Method ◽  
Late Onset ◽  
Serum Levels ◽  
Maternal Serum ◽  
Severe Preeclampsia ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Difference

The purpose of this study is to know the difference average of maternal serum levels of HIF-1α between early-onset and late-onset severe preeclampsia. This study used a cross sectional comparative study design that conducted in Februari 2020 - Agustus 2020 in the SMF / Obstetrics and Gynecology department of RSUP dr. M. Djamil Padang, RSUD Achmad Mochtar, RSUD Pariaman, RSUD M Zein Painan. We used consecutive sampling method which consists of 60 pregnant women who fulfill the inclusion and exclusion criteria. They were divided into two groups early-onset severe preeclampsia and late-onset severe preeclampsia. HIF-1α tests were done using ELISA method. The average of maternal serum levels of HIF-1α in late-onset severe preeclampsia is found to be the highest when compared to the early-onset severe preeclampsia, 1,37 ± 1,08 ng/ml vs 0,69 ± 0,11 ng/ml. This difference is significant with the Mann-whitney non parametrical statistical test (p <0.05). There is a significant difference average of maternal serum levels of HIF-1α between early-onset and late-onset severe preeclampsiaKeywords: early onset severe preeclampsia, late onset preeclampsia late onset, maternal serum levels of  HIF-1α

scholarly journals The Difference in Maternal Serum Hypoxia-Inducible Factors-1α Levels between Early Onset and Late-Onset Preeclampsia

2019 ◽  
Vol 7 (13) ◽  
pp. 2133-2137 ◽  
Author(s):  
Roza Sriyanti ◽  
Johanes C. Mose ◽  
Masrul Masrul ◽  
Netti Suharti
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Hypoxia Inducible Factors ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Difference ◽  
Early Onset Preeclampsia

BACKGROUND: Preeclampsia can be divided into early (EOPE) and late (LOPE) onset preeclampsia. Preeclampsia is related to the failure of placentation. Accumulation of hypoxia-inducible factors (HIF)-1α is commonly an acute and beneficial respond to hypoxia, while chronically elevated is associated with preeclampsia. AIM: This study aims to evaluate the serum levels of HIF-1α in preeclampsia and normal pregnancy, and to compare the difference between early-onset and late-onset preeclampsia. METHODS: A cross-sectional comparative study was conducted among a total of 69 pregnant women at ≥ 20 weeks of gestation, were recruited at obstetrics and gynaecology department at Dr M. Djamil Padang Hospital, network hospitals, health centres. They were divided into three groups early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy. Preeclampsia was diagnosed using International Guidelines. Data were analysed by SPSS 24 program; data are presented as median and range or as mean ± standard deviation. One-way ANOVA test was used to determine the relationship between HIF-1α levels with the onset of preeclampsia. RESULTS: The results showed that the mean maternal serum HIF-1α levels in early-onset preeclampsia (EOPE), late-onset preeclampsia (LOPE), and normal pregnancy were 1366.96 ± 733.40 pg/ml, 916.87 ± 466.06 pg/ml, and 716.77 ± 541.08 pg/ml. Serum HIF-1α levels were higher in early-onset preeclampsia (EOPE), and late-onset preeclampsia (LOPE) compared to normal pregnancy. Among preeclampsia patients, serum HIF-1α was higher in EOPE than LOPE women. Statistical analysis revealed a significant difference in mean maternal serum HIF-1α between early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy (p < 0.05). CONCLUSION: This study concluded that there is a significantly different level of HIF-1α between in early-onset preeclampsia, late-onset preeclampsia and normal pregnancy. Early-onset preeclampsia is the highest levels of serum HIF-1α.

scholarly journals Differences in levelFms-Like Tyrosine Kinase-1 (sFlt-1), soluble Endoglin (s-Eng), and Placental Growth Factor (PIGF) between Early Onset Preeclampsia and Late Onset Preeclampsia

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Lita Nafratilova ◽  
Yusrawati Yusrawati ◽  
Irza Wahi
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Intrinsic Factors ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design ◽  
Early Onset Preeclampsia

Early Onset Preeclampsia (EO-PE) is preeclampsia that develops before 34 weeks 'gestation, caused by intrinsic factors, while Late Onset Preeclampsia (LO-PE) is preeclampsia that develops after 34 weeks' gestation due to extrinsic and maternal factors. There is an increased production of antiangiogenic factors (sFlt-1, s-Eng and PIGF) contribute to pathophysiology of preeclampsia.This study aims to measure the difference of sFlt-1, sEng, PIGF levels between EO-PE and LO-PE. This was an observational study with cross sectional design conducted at Dr. M. Djamil, TK Hospital. III dr. Reksodiwiryo and Biomedical Laboratory FK Unand Padang from August 2017 to August 2018. The sample of this study were 26 severe preeclampsia women : 13 (EO-PE)  and 13 (LO-PE), selected using consecutive sampling. Levels of sFlt-1, sEng, PIGF were examined using the enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was performed using unpaired t test and Mann-Whitney Test. Results shown that serum levels of sFlt-1 and sEng in (EO-PE)  were 9.51 ± 0.71 ng / L, 1.44 ± 0.06 ng / mL, 5.79 ± 0.42 ng / mL while in PEAL it was 8, 89 ± 0.78 ng / mL, 1.35 ± 0.14 ng / mL, 6.72 ± 0.76. There were a significant difference with a value of p <0.05. The conclusion of this study is that the levels of sFlt-1 and sEng are higher in (EO-PE)  than(LO-PE)and PIGF levels was lower in (EO-PE) compared to (LO-PE)

scholarly journals PERBEDAAN RERATA KADAR AKTIVIN A SERUM MATERNAL ANTARA PREEKLAMSIA BERAT DENGAN BUKAN PREEKLAMSIA BERAT

2019 ◽  
Vol 1 (1) ◽  
pp. 30-37
Author(s):  
Dovy Djanas ◽  
Bayu Pramudyo Ariwibowo ◽  
Hafni Bachtiar
Keyword(s):  
Serum Levels ◽  
Activin A ◽  
Maternal Serum ◽  
Severe Preeclampsia ◽  
Inclusion Criteria ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Tnf Α ◽  
The Mean ◽  
In Pregnancy

At the start of preelampsia there is a failure of cytotrophoblst invasion into the maternal spiral arteries that will lead to decreased uteroplacetal perfusion which will be followed by the failure of the unit fetoplacenter to get enough oxygen from the room intervillous that ultimately lead to a state of hypoxia in placenta. This will cause the expenditure of TNF-α dan IL-1β from placenta and a factors called hypoxia-inducible transcription factors that will spur the trophoblast to produce activin A lot more. This research was conducted by cross sectional method in maternal room of obstetrics and gynecology department of Central General Hospital of Dr. M. Djamil Padang from August 2015 until February 2016 with 20 patients of severe preeclampsia and 20 patients not severe preeclampsia, who met inclusion criteria and there is no exclusion criteria. Then performed statistical analysis using Mann-Whitney test to determine difference in mean maternal activin A serum levels of severe preeclampsia and not severe preeclampsia. The mean maternal serum levels of activin A in severe preeclampsia is 32,55 ± 1,84 ng/ml and in pregnancy with no severe preeclampsia is 8,59 ± 0,59 ng/ ml. Difference in mean maternal serum level of activin A in the two groups was statistically significant (p=0,001). Ma-ternal serum activin A levels is significantly higher in severe preeclampsia than pregnancy with no severe preeclampsia.Keywords: Activin A, severe preeclampsia, not severe preeclampsia

scholarly journals Perbedaan Rerata Kadar Soluble Fms-Like Tyrosine Kinase-1 (Sflt-1) Serum pada Penderita Early Onset, Late Onset Preeklampsia Berat / Eklampsia dan Kehamilan Normal

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Laila Rahmi ◽  
Rahmatina B. Herman ◽  
Yusrawati Yusrawati
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Severe Preeclampsia ◽  
Cross Sectional ◽  
Post Hoc ◽  
Endothelial Growth Factor

AbstrakPreeklampsia merupakan sumber utama morbiditas dan mortalitas ibu di seluruh dunia. Kegagalan pengaturan dan ketidakseimbangan agen vasoaktif proangiogenik dan antiangiogenik plasenta, soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF) dan placental growth factor (PlGF) memainkan peran penting dalam patogenesis preeklampsia. Tujuan penelitian ini adalah menentukan perbedaan rerata kadar sFlt-1 serum pada penderita early onset, late onset preeklampsia berat/ eklampsia dan kehamilan normal. Penelitian dilakukan di RSUP Dr. M. Djamil, RS TK. III dr. Reksodiwiryo dan Laboratorium Biologi Molekuler Fakultas Kedokteran Universitas Andalas Padang dari Februari sampai  Desember 2014 dengan desain cross sectional. Subjek berjumlah 84 orang, terdiri dari tiga kelompok, yaitu kelompok early onset preeklampsia berat/ eklampsia, late onset preeklampsia berat/ eklampsia, dan kehamilan normal sebagai kelompok kontrol yang diambil dengan teknik consecutive sampling. Darah dikumpulkan dari subjek penelitian dengan cara intravena kemudian diukur dengan metode ELISA. Rerata kadar sFlt-1 pada kelompok early onset, late onset preeklampsia berat/ eklampsia dan kehamilan normal secara berturut-turut adalah 4,69±0,96 ng/ml, 2,39±0,57 ng/ml, dan 1,23±0,42 ng/ml. Perbedaan ini sangat signifikan dengan uji statistik ANOVA (p<0,05) dan uji Post Hoc Test Multiple Comparisons. Kesimpulan penelitian adalah terdapat perbedaan yang sangat signifikan antara kadar sFlt-1 serum pada kelompok early onset preeklampsia berat/ eklampsia, late onset preeklampsia berat/ eklampsia dan kehamilan normal.Kata kunci: sFlt-1, antiangiogenik, preeklampsia berat/ eklampsia, kehamilan normal AbstractPreeclampsia is a major cause maternal morbidity and mortality in the world. Failure regulation and imbalance of vasoactive agents and antiangiogenic proangiogenik placenta, soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) have an important role in the pathogenesis of preeclampsia. The objective of this study was to determine the differences between the mean serum levels of sFlt-1 in patients with early onset, late onset severe preeclampsia/eclampsia and normal pregnancy. This study was conducted in Dr. M. Djamil hospital, dr. Reksodiwiryo TK. III hospital and Biology Moleculer Laboratory Medicine Faculty of Andalas University Padang from February until December 2014 with a cross sectional design. The total subjects were 84 persons, consist of three groups, there was early onset severe preeclampsia/ eclampsia, late onset severe preeclampsia/ eclampsia and normal pregnancy as control group. The subjects were selected by consecutive sampling technique. The blood was collected by intravenous,  then sFlt-1 serum measured by ELISA. The mean levels of sFlt-1 in the early onset group, late onset severe preeclampsia/ eclampsia group and normal pregnancy group were 4.69±0.96 ng/ml, 2.39±0.57 ng/ml and 1.23±0.42 ng/ml. This difference very significant by ANOVA statisctical test (p<0,05) and Multiple Comparisons Post Hoc Test. The conclusion of this study is very significant differences between serum levels of sFlt-1 in early onset severe preeclampsia/ eclampsia group, late onset severe preeclampsia/ eclampsia on  normal pregnancy.Keywords: sFlt-1, antiangiogenic, severe preeclampsia/ eclampsia, normal pregnancy

scholarly journals The correlation between Leptin Levels and Onset of Preeclampsia

2020 ◽  
Vol 4 (2) ◽  
pp. 170-175
Author(s):  
Roza Sriyanti ◽  
Johanes C. Mose ◽  
Masrul Masrul ◽  
Netti Suharti
Keyword(s):  
Early Onset ◽  
Sampling Method ◽  
Late Onset ◽  
Normal Pregnancy ◽  
Statistical Test ◽  
Elisa Method ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Consecutive Sampling ◽  
Early Onset Preeclampsia

The purpose of this study is to find the correlation between leptin levels and the onset of preeclampsi. This study used a cross sectional comparative study design that conducted in May 2018 - April 2019 in the SMF / Obstetrics and Gynecology department of RSUP dr. M. Djamil Padang, RSUD Achmad Mochtar, RSUD Solok, RST Reksodiwiryo. We used consecutive sampling method which consists of 69 pregnant women who fulfill the inclusion and exclusion criteria. Leptin level tests were done using ELISA method. The average level of leptin in early-onset preeclampsia is found to be the highest when compared to the late-onset preeclampsia and normal pregnancy, 64.07 ± 78.27 vs. 30.46 ± 31.99 vs. 16.61 ± 24.49. This differentiation is highly significant with the ANOVA statistical test (p <0.05). There is a significant correlation between leptin levels with the onset of preeclampsia.Keywords: preeclampsia early onset, preeclampsia late onset, leptin levels

The correlation between Leptin Levels and Onset of Preeclampsia

2020 ◽  
Vol 4 (2) ◽  
pp. 139-144
Author(s):  
Roza Sriyanti ◽  
Johanes C. Mose ◽  
Masrul Masrul ◽  
Netti Suharti
Keyword(s):  
Early Onset ◽  
Sampling Method ◽  
Late Onset ◽  
Normal Pregnancy ◽  
Statistical Test ◽  
Elisa Method ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Consecutive Sampling ◽  
Early Onset Preeclampsia

The purpose of this study is to find the correlation between leptin levels and the onset of preeclampsi. This study used a cross sectional comparative study design that conducted in May 2018 - April 2019 in the SMF / Obstetrics and Gynecology department of RSUP dr. M. Djamil Padang, RSUD Achmad Mochtar, RSUD Solok, RST Reksodiwiryo. We used consecutive sampling method which consists of 69 pregnant women who fulfill the inclusion and exclusion criteria. Leptin level tests were done using ELISA method. The average level of leptin in early-onset preeclampsia is found to be the highest when compared to the late-onset preeclampsia and normal pregnancy, 64.07 ± 78.27 vs. 30.46 ± 31.99 vs. 16.61 ± 24.49. This differentiation is highly significant with the ANOVA statistical test (p <0.05). There is a significant correlation between leptin levels with the onset of preeclampsia.Keywords: preeclampsia early onset, preeclampsia late onset, leptin levels

scholarly journals Placental Growth Factor Level is Lower in Early-Onset Preeclampsia, while Tumor Necrosis Factor Alpha Level does not Show any Difference between Early and Late Onset Preeclampsia

2016 ◽  
Author(s):  
Christofani Ekapatria
Keyword(s):  
Serum Level ◽  
Early Onset ◽  
Late Onset ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
District Hospitals ◽  
Tnf Α ◽  
Kolmogorov Smirnov ◽  
The Difference ◽  
Early Onset Preeclampsia

Objective: To analyze the difference of PlGF and TNF-α serum level between early-onset and late-onset preeclampsia. Method: This is a cross-sectional analytic comparative study comparing serum level of PlGF and TNF-α between groups with earlyand late-onset preeclampsia. Each group consists of 32 subjects who met inclusion criteria and presented to Dr. Hasan Sadikin Hospital or its district hospitals in September - November 2012. Statistical analysis was performed with Kolmogorov Smirnov test, Saphiro-Wilk test, and non-parametric Mann-Whitney test. Result: Mean of PlGF serum level in the group with early-onset preeclampsia is 53.0344±38.07140 pg/ml, while mean of which in the group with late-onset preeclampsia is 241.8063±192.8373 pg/ml (p

scholarly journals Effect of esomeprazole on maternal serum soluble fms-like tyrosine kinase-1 and endoglin in patients with early-onset preeclampsia

2021 ◽  
Vol 99 (1) ◽  
Author(s):  
Ahmed M Abbas ◽  
Yousra M Othman ◽  
Mohamad M Abdallah ◽  
Noura H. Abd Ellah ◽  
Hanan G. Abdel Azim ◽  
...  
Keyword(s):  
Placebo Group ◽  
Tyrosine Kinase ◽  
Early Onset ◽  
Serum Levels ◽  
Maternal Serum ◽  
Termination Of Pregnancy ◽  
Elisa Testing ◽  
Significant Difference ◽  
Fetal Complications ◽  
Early Onset Preeclampsia

Objective: This study evaluates the effect of esomeprazole on the maternal serum levels of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) in patients with early-onset preeclampsia.Methods: A randomized, double-blind, placebo-controlled trial was carried out in a tertiary University hospital between March 2018, and September 2019 (Clinical Trials.Gov: NCT03213639). The study included women between 28 and 31+6 weeks gestational age who had been diagnosed as preeclampsia without severe features. They were randomly assigned in a 1:1 ratio into an esomeprazole group, which received esomeprazole 40 mg orally once a day, and a placebo group, which received one placebo tablet daily. Blood samples were obtained to assess levels of serum sFlt-1and sEng using ELISA testing. The primary outcome was the difference between the mean serum level of sFlt-1 and sEng at the start of treatment and at the termination of pregnancy in both groups.Results: Eighty-eight patients were randomly assigned into both groups (44 in each). No statistically significant difference was found in the levels of sFlt-1 between both groups at admission and termination of pregnancy. The number of days of treatment for the esomeprazole group was slightly longer than the placebo group (11.4±9.4 vs. 10.3±6.3 days, P=0.515). No statistically significant difference in the rate of maternal and fetal complications occurred between the two groups. No side effects from the study medications were reported.Conclusions: Esomeprazole, at the dosage used in this study did not effectively lower the serum levels of sFlt-1 and sEng in patients with early-onset preeclampsia. Furthermore, it did not prolong the duration of pregnancy, nor did it decrease maternal or fetal complications.

scholarly journals Ovarian Reserve In Infertile Women with and without Endometriosis Measured with Anti Müllerian Hormone

2016 ◽  
Author(s):  
Naivah Harharah
Keyword(s):  
Ovarian Reserve ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Blood Samples ◽  
Infertile Women ◽  
Significant Difference ◽  
The Mean ◽  
The Difference

Objective: To compare serum Anti Müllerian Hormone (AMH) levels in infertile women with and without endometriosis, and to determine the mean levels of serum AMH in every stage of endometriosis. Method: We performed a cross-sectional study. Sixty-eight subjects who have undergone laparoscopy and fulfilled both inclusion and exclusion criteria are recruited consecutively. They are divided into two groups, namely group with endometriosis and without endometriosis. Blood samples are taken from each subject before laparoscopy, where serum AMH levels are then measured. The difference in mean levels of each group are tested with Mann-Whitney test. Result: The mean levels of serum AMH were significantly lower in the endometriosis group than those in the group without endometriosis (2.30 1.8 ng/ml vs 3.75 2.13 ng/ml; p=0.005). Using Kruskal-Wallis test, it was found that there was a statistically significant difference among endometriosis groups based on the severity of endometriosis. There was no significant difference in the mean serum AMH levels between the minimal-mild endometriosis group and without endometriosis group (p=0.34), but the mean levels of serum AMH were significantly lower in the moderate-severe endometriosis compare to the group without endometriosis (p

scholarly journals Differences in Multimodal EEG and Clinical Correlations Between Alzheimer’s Disease and Frontotemporal Dementia

2021 ◽  
Author(s):  
Nan Lin ◽  
Jing Gao ◽  
Chenhui Mao ◽  
Heyang Sun ◽  
Qiang Lu ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Spectral Analysis ◽  
Frontotemporal Dementia ◽  
Clinical Data ◽  
Early Onset ◽  
Late Onset ◽  
Clinical Severity ◽  
Significant Difference ◽  
The Difference

Abstract Background. Alzheimer’s disease (AD) and frontotemporal dementia (FTD) are the two main types of dementia. We aim to investigate the difference between AD and FTD by use of multimodal EEG analyses. Additionally, the difference in correlations between EEG and clinical data was also investigated.Methods. Thirty-one patients diagnosed with AD and 15 patients with FTD were recruited (2008.1-2020.2), along with 24 healthy controls. Clinical data were reviewed. EEG microstate analysis, spectral analyses, and connectivity analysis were performed. Results. Microstate duration was increased in AD for microstate B and increased in FTD for microstate A compared to controls. Correspondingly, microstate C occurrence was decreased in both dementia groups, compared to control group. After divided into early onset and late onset AD, increased mean duration and reduced mean occurrence were observed in early onset AD, compared to late onset AD, with no significant difference in visual EEG score. CSF Aβ42 was correlated to microstate B coverage in AD (r = -0.833, P = 0.010), and microstate D occurrence in FTD (r = 0.786, P = 0.021). ADL and MMSE were also related to visual EEG score and microstate, but for different variables in the two dementia groups. Spectral analysis revealed decreased power in 8-30 Hz and increased power in delta band in both dementia. AD had higher spectral power in the temporal region, compared to FTD. Reduced alpha and beta coherences were demonstrated in AD in bilateral frontal, fronto-temporal, and fronto-occipital connections, and in FTD in the right frontal and fronto-temporal connections. Conclusions. Multimodal EEG analyses show different results between AD and FTD. Reduced coherence is across more brain areas in AD, including intra-anterior and anterior-posterior regions, compared to FTD, which only had frontal-temporal connectivity involved. Spectral analysis revealed a general EEG slowing. Increased microstate duration and decreased occurrence may be attributed to EEG slowing, for different classes in different types of dementia. Microstate may be more sensitive than visual EEG inspection. The correlations with clinical severity and biomarkers indicate that EEG is a potential biomarker for diagnosis and disease assessment.

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