scholarly journals Karakterisasi Morfologi Populasi Etlingera elatior (Jack) R.M.Sm. (Zingiberaceae) di Sumatera Barat

2018 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
Zola Anjelia Putri ◽  
Nurainas Nurainas ◽  
Syamsuardi Syamsuardi

Research about morphological characterization of Etlingera elatior  (Jack) R.M.Sm. (Zingiberaceae) was conducted from August to December 2016 in West Sumatra regions (Kepulauan Mentawai, Solok, and Padang Pariaman) and Padang city. Etlingera elatior has been used as traditional medicines for several diseases. Etlingera elatior was used for the treatment of various diseases. Effectivityof this plant depend on the preciseus in of the determinating variants type. The aim of this research was analyzed morphological variation among populations of Etlingera elatior. Survey and direct sampling method were used and continued by measurement of morphological characters of Etlingera elatior. Classification analyzed by cluster analysis with PAST program. Based on differentiation of the bract color of flowers, four variants of Etlingera elatior in West Sumatra were clarified those were 'red', 'pink', 'pale pink', and 'white' variant. The result of cluster analysis of 53 individuals Etlingera elatior indicated that many of 'white' and the 'red' variant were clustered in the same main cluster and individuals of 'pale pink' variant mostly clustered to 'red' variant and some of them were existed in the 'pink’ variant.

2019 ◽  
Vol 25 (2) ◽  
pp. 15
Author(s):  
Sumilah Sumilah ◽  
Nirmala F. Devy ◽  
Hardiyanto Hardiyanto

<p>The diversity of sweet potatoes from Agam and Solok District of West Sumatra is known to be very high, it causes somedifficulties in taxonomic research. The diversity analysis based on the leaf and flower morphology has not done before in local sweet potato from Agam and Solok District. The purpose of this study was to determine the diversity of local varieties of sweet potato from West Sumatra based on leaves and flowers morphological characters. The analysis was conducted on 21 accessions collected from West Sumatra regencies and germplasm collection. The Morphological characterization was done based on CIP/AVRDC/IBPGR descriptor list. The data of morphological character was analyzed using Primary Component Analysis (PCA) and grouping analysis using Cluster Analysis through SAHN method on NTSys program, with UPGMA as similarities criteria. The results showed that the first four principal components contributed 89.6% to the plant diversity. The sweet potatoes were divided into 2 clusters, with the main distinguishing character was the existence of plant flowers. Information on genetic diversity and the pattern of relations between accessions will be very useful for the West Sumatra people in developing superior sweet potatoes.</p>


2016 ◽  
Vol 55 ◽  
pp. 57-67
Author(s):  
A. Muthu Kumar ◽  
Pratibha Sharma

The morphological characterization was carried out for 5 isolates of Trichoderma harzianum and 7 isolates of Trichoderma viride and tested for their biocontrol efficacy. The isolates belonging to T.harzianum were analogous in colony colour, culture smell, mycelial colour, conidiation, conidial shape, conidial wall and conidial colour. Correspondingly the isolates of T.viride showed certain similarity in colony colour, colony edge, culture smell, conidiophore branching, conidial wall, conidial colour and chlamydospores. Inter specific differences through cluster analysis based on morphological characters grouped the twelve isolates into three major clusters where all the isolates of T.harzianum formed a single cluster while the isolates of T.viride were bifurcated into two groups. The clustering was substantiated by similarity index which showed maximum similarity among T.harzianum isolates with only less than 20% variation among themselves. Similarly the clusters having isolates of T.viride also had less variation within them. The biocontrol efficacy of these twelve isolates of Trichoderma was experimented by dual culture test under laboratory condition and there existed some relation between the biocontrol efficacy of these isolates and morphology.


2021 ◽  
Vol 12 (2) ◽  
pp. 110
Author(s):  
Febri Yuda Kurniawan ◽  
Anindita Della Rosa Riyadi

Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.


2002 ◽  
Vol 92 (9) ◽  
pp. 986-996 ◽  
Author(s):  
Pedro Talhinhas ◽  
S. Sreenivasaprasad ◽  
João Neves-Martins ◽  
Helena Oliveira

Anthracnose, caused by Colletotrichum sp., is a serious problem of lupins (Lupinus spp.) worldwide. Morphological characters and molecular markers were used to characterize 43 Colletotrichum isolates from lupins, 8 isolates from other hosts, and 18 reference isolates representing related Colletotrichum spp., to assess the pathogen diversity and resolve its taxonomy. All lupin Colletotrichum isolates tested positive with C. acutatum-specific polymerase chain reaction (PCR) and did not test positive with C. gloeosporioides-specific PCR. Spore shape and colony diameter as well as insensitivity to benomyl grouped the lupin anthracnose isolates closer to C. acutatum than to C. gloeosporioides. Analysis of internal transcribed spacer (ITS) sequences of 57 Colletotrichum isolates grouped all lupin isolates with C. acutatum and distinct from C. gloeosporioides. Further, tub2 and his4 sequences revealed groups concordant with ITS, reducing the excessive dependence on the latter. Arbitrarily primed-PCR and amplified fragment length polymorphism analyses revealed intraspecific subgroups, but neither was useful to decipher species level relationships. ITS, tub2, and his4 results strongly support designating lupin anthracnose pathogen as C. acutatum or its subspecies. Most Colletotrichum isolates from lupins from worldwide locations are genetically homogeneous and form a distinct subgroup within C. acutatum. Present results also underline the potential of the C. acutatum-specific PCR for routine pathogen diagnosis.


2018 ◽  
Vol 53 (3) ◽  
pp. 359-373
Author(s):  
Patricia Diez De Ulzurrun ◽  
María B. Acedo ◽  
María E. Garavano ◽  
Valeria Gianelli ◽  
Verónica N. Ispizúa

Morphological characterization of Conyza blakei, Conyza bonariensis var. bonariensis, Conyza sumatrensis var. sumatrensis and Conyza lorentzii in the southeast of Buenos Aires (Argentine). The genus Conyza Less. includes ruderal species, which grow of ruderal habitat, roadsides and railways. They have also been cited as pasture weeds, annual and perennial crops under direct sowing (SD) systems, adapting to these slightly disturbed environments since they are sensitive to soil removal. The taxonomic identification of Conyza species linked to morphological characters is difficult in vegetative stages, since this genus has highly related and polymorphic species. With the objective of morphologically characterizing Conyza blakei, C. bonariensis var. bonariensis, C. lorentzii and C. sumatrensis var. sumatrensis were recorded 19 morphological characters (12 vegetative and 7 reproductive) qualitative and quantitative, in 35 plants of each species. These characters were analyzed by Multivariate Analysis (Analysis of Components and Main Coordinates). The morphological characterization made it possible to detect phenotypic differences between the Conyza species analyzed. The analysis of main coordinates allowed to differentiate the species both at a reproductive and vegetative level, while the analysis of main components only allowed to differentiate the species in reproductive stage.


1999 ◽  
Vol 89 (5) ◽  
pp. 414-420 ◽  
Author(s):  
S. Banniza ◽  
A. A. Sy ◽  
P. D. Bridge ◽  
S. A. Simons ◽  
M. Holderness

Isolates of Rhizoctonia solani were obtained from plant and soil samples that had been systematically collected in a field experiment in Côte d'Ivoire to study the diversity of the pathogen and the influence of three different rice rotations on the pathogen population. Characterization by morphology, anastomosis testing, pathogenicity testing, and restriction fragment length polymorphisms (RFLPs) of AT-rich DNA (AT-DNA) showed that there were no differences in isolates from different experimental plots, suggesting that the soil as well as the plant population of the fungus was indistinguishable throughout the experiment and was not influenced by crop rotation. Analysis of AT-DNA showed that the isolates obtained from plant material and one from soil shared a distinct banding pattern, identical with the AT-DNA RFLP obtained for the reference strain of anastomosis group 1 (AG-1). The remaining soil isolates produced a consistent RFLP pattern that was distinct from that of the plant isolates. Morphological characterization of isolates produced two major clusters consisting of the same groups of isolates as found by AT-DNA RFLP. Diversity in morphological characters was much higher in plant than in soil isolates and indicated that the population might consist of several clones. Anastomosis testing revealed that soil as well as plant isolates were able to fuse with the tester strain of AG-1. Significant differences in disease severity were observed between the two groups of isolates in pathogenicity tests on rice plants, with plant isolates being distinctively more virulent.


Zootaxa ◽  
2019 ◽  
Vol 4624 (1) ◽  
pp. 1-22 ◽  
Author(s):  
GUIDOMAR O. SOLEDADE ◽  
JUSTIN A. SCIOLI ◽  
MARIANA TEROSSI ◽  
FERNANDO L. MANTELATTO ◽  
ALEXANDRE O. ALMEIDA

The snapping shrimp Alpheus macrocheles (Hailstone, 1835b) is widely distributed across the Atlantic Ocean and was originally described from Hastings, England, based on a very brief description with limited morphological details and diagnostic illustrations. The morphologically similar A. amblyonyx Chace, 1972, type locality Quintana Roo, Mexico, is a western Atlantic taxon. The superficial description of A. macrocheles by Hailstone and the high similarity with A. amblyonyx has led to taxonomic confusion regarding the precise identification of these species. Thus, our objective was to reexamine these two species, based on a combined analysis of morphological—including a redescription for A. macrocheles and new morphological characterization of A. amblyonyx—and molecular data. In total, 42 morphological characters were analyzed, in addition to a phylogenetic analysis of 16S ribosomal RNA (rRNA) gene sequences. A Bayesian Inference analysis recovered A. macrocheles and A. amblyonyx as distinct clades supporting the taxonomic distinction between them. The tree topology suggests that A. macrocheles is more closely related to A. crockeri (Armstrong, 1941) and A. puapeba (Christoffersen, 1979) than to A. amblyonyx, which forms the sister group to a well-supported clade containing these three species. This contribution conclusively distinguishes between A. macrocheles and A. amblyonyx and points out the need for a comprehensive review involving all species of the larger A. macrocheles group. 


Author(s):  
Silvio de J. Martínez Medina ◽  
Raúl Barbón Rodríguez, Rafael Gómez-Kosky, Novisel Veitía Rodríguez ◽  
María Esther González Vega ◽  
Orlando Saucedo Castillo ◽  
Eduardo Fidel Héctor Ardisana ◽  
...  

Sorghum [Sorghum bicolor (L.) Moench] is an important crop after wheat, corn, rice and barley. The aim of this research was to study morphological characterization of the plant population of grain sorghum cultivar CIAP 132R-05 regenerated via somatic embryogenesis under semi controlled conditions in green house.  Plants regenerated from somatic embryos were compared to plants grown from botanical seeds. Both populations of plants were characterized morphologically, taking in account quantitative traits (plant height (cm), stem diameter, number of active leaves per plant, limb length and width, number of active roots per plant, length of the roots (cm), fresh weight (gMF), foliar area (dm2), number of shoots per plant) and qualitative traits (albino or variegated plants, color of the leaves). Quantitative characteristics of both populations corresponded with those listed in the National Register of Commercial Varieties of Cuba. However; the population of plants derived from somatic embryos showed significantly higher values ​​for plant height, stem diameter, limb length and width, which may be associated with the physiological rejuvenation produced by the effect of in vitro culture. This allowed to determine the phenotypic stability of the regenerated plants via somatic embryogenesis, by assessing morphological characters in field conditions. The results can be applied to the in vitro propagation of elite plants selected as a product of conventional breeding programs and obtained through the use of different biotechnological methods.


2021 ◽  
Vol 4 (1) ◽  
pp. 55-64
Author(s):  
Intan Sari ◽  
Henri Henri ◽  
Eka Sari ◽  
Suharyanto Suharyanto

Ginseng plant (Phytolacca octandra L.) is a wild plant that has the potential to be used as medicine. The morphological characteristics of ginseng plants in Indonesia are different. Therefore, it is necessary to carry out research aimed at characterizing the morphology and phytochemical screening of ginseng (P. octandra L.) leaves and tubers. The research was conducted first by observing morphological characters and screening phytochemical compounds such as alkaloid test, flavonoid test, phenol test, tannin test, saponin test, terpenoid test, and steroid test. The results of the research on the morphological characterization of ginseng (P. octandra L.) were: having a taproot which was modified into a tuber, a round, smooth, red color, pointed leaf shape, the base and tip of the leaf were pointed, buni fruit type, round flat, colored blackish-purple when ripe, a compound flower is located at the end of the branch and has flat, round seeds. Ginseng tubers have triterpenoid and saponin compounds, while the leaves only have saponin compounds. The potential development of ginseng (P. octandra L.) is currently limited as traditional medicine by local people. This is due to limited knowledge in its development efforts and unknown phytochemical content.


2014 ◽  
Vol 50 (No. 2) ◽  
pp. 177-184 ◽  
Author(s):  
B. Gixhari ◽  
M. Pavelková ◽  
H. Ismaili ◽  
H. Vrapi ◽  
A. Jaupi ◽  
...  

In order to investigate the genetic diversity present in the pea germplasm stored in the Albanian genebank, we analyzed 28 local pea genotypes of Albanian origins for 23 quantitative morphological traits, as well as 14 retrotransposon-based insertion polymorphism (RBIP) molecular markers. The study of morphological characters carried out during three growing seasons (2010, 2011 and 2012) had the objective of characterization of traits useful in breeding programs. RBIP marker analysis revealed the genetic similarity in range from 0.06 to 0.45. ANOVA, principal component analysis (PCA) and cluster analysis was used to visualize the association among different traits. Most of the quantitative morphological traits showed significant differences. PCA and cluster analysis (Ward&rsquo;s method) carried out for morphological traits divided the local pea genotypes into three clusters. Finally, the study identified the agronomicaly important traits which will facilitate the maintenance and agronomic evaluation of the collections.


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