scholarly journals Arbuscular mycorrhizae colonization dynamic in Iris pseudacorus L. during second year after inoculation and planting in field

2021 ◽  
Vol 26 (3) ◽  
pp. 2631-2639
Author(s):  
IOANA CRIȘAN ◽  
◽  
ROXANA VIDICAN ◽  
ADRIANA MOREA ◽  
VIORICA LAGUNOVSCHI ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Vascular Plant ◽  
Functional Integration ◽  
Microbial Inoculants ◽  
Higher Plant ◽  
Temporal Gradient ◽  
Iris Pseudacorus ◽  
Market Expansion ◽  
Microscopic Evaluation ◽  
Second Year

In the last few years was observed an unprecedented market expansion of microbial inoculants for agriculture and environmental applications. Fungi from the phylum Glomeromycota can establish symbiosis with roots of more than 70% vascular plant species, ensuring enhanced nutrient uptake. Aim of this study was to assess functional integration of inoculated arbuscular mycorrhiza and implications for success of symbiose in field conditions and host fitness. Iris pseudacorus was used as perennial model plant for study of seasonal continuity and persisting effects in second year after inoculation. During 2019 was conducted Trouvelot microscopic evaluation for 1080 stained root samples and parameters were obtained using Mycocalc. Results showed that colonization was significantly influenced by phenophase. Inoculated plants presented values with 1.56-14.49% higher. Plant height correlated significantly positive with inoculation (r=0.336*). Results showed that colonization parameters are useful indicators in tracking symbiose across a temporal gradient.

Arbuscular mycorrhizae of American ginseng (Panax quinquefolius) in cultivated field plots: plant age affects the development of a colonization lag phase

1999 ◽  
Vol 77 (7) ◽  
pp. 1028-1034 ◽  
Author(s):  
T P McGonigle ◽  
J P Hovius ◽  
R L Peterson
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizae ◽  
Dry Mass ◽  
American Ginseng ◽  
Panax Quinquefolius ◽  
Root Turnover ◽  
Lag Phase ◽  
Seed Collection ◽  
Second Year ◽  
Hyphal Coils

Temporal patterns of colonization of roots of perennials by arbuscular mycorrhizal fungi are poorly understood because annual crops are more often studied. The objective was to monitor in detail the growth and mycorrhizae of the perennial American ginseng (Panax quinquefolius L.) over the first 2 years after seeding. In particular, the extent of carry-over of colonization into the second year was examined. Delayed germination of some seeds caused by chance-driven variability in the time needed for seed maturation after seed collection provided an opportunity to compare first- and second-year plants under identical conditions in the second year. For all plants, development of arbuscules rose to a maximum in mid-August, falling partially thereafter. Production of hyphal coils proceeded more slowly but steadily, so that end-of-season colonization was composed equally of arbuscules and hyphal coils. Between seasons, taproot dry mass was reduced by 18%, whereas lateral root length increased by 15%. These changes were probably caused by root turnover, which left little initial colonization of roots at the start of the second year. Strikingly, second-year plants did not exhibit a colonization lag phase, whereas a lag of 43 days was seen for the first-year plants alongside. Possible reasons for this difference are discussed.Key words: perennial, arbuscules, hyphal coils, taproot, Ontario.

scholarly journals Growth and Nutrient Uptake Responses of Kinnow to Vesicular Arbuscular Mycorrhizae

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
M. H. Shamshiri ◽  
K. Usha ◽  
Bhupinder Singh
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Growth Parameters ◽  
Cumulative Effect ◽  
Flowering Phenology ◽  
Trunk Diameter ◽  
Beneficial Effects ◽  
Canopy Volume ◽  
Vesicular Arbuscular ◽  
Second Year ◽  
The Difference

In a field experiment, three-year-old Kinnow trees budded on Jatti Katti (C. jambhiri) rootstock were inoculated by three different arbuscular mycorrhiza (AM), Glomus manihotis (T1), Glomus mosseae (T2), and Gigaspora gigantia (T3), separately or in combination (T4). Microscopic assessment of AM colonization on Kinnow roots showed a relatively lower level of infection by different species when inoculated separately than when inoculaed together. Application of AM improved growth parameters like plant height, canopy volume, mean leaf area, and number of new shoots per plant but had no effect on trunk diameter, number of leaf per new shoot and new shoot diameter. Flowering phenology was also altered by AM infection. The infected plants produced more flowers which despite abscission caused an increase in the initial number of fruits. AM inoculation increased plant phosphorous in T3, potassium in T1, T2, T3, calcium in T2, T3, T4, and the AM-nutrient relationship did not reveal any generalized pattern. Inoculated plants, however, had higher concentration of different elements by the second year of the experiment indicating towards the continuous and cumulative effect of AM infection on plant nutrient accumulation. Further, the beneficial effects of AM on Kinnow were not related to the difference in the species of mycorrhiza.

The mycorrhizal status, root anatomy, and phenology of plants in a sugar maple forest

1988 ◽  
Vol 66 (6) ◽  
pp. 1153-1173 ◽  
Author(s):  
Mark C. Brundrett ◽  
Bryce Kendrick
Keyword(s):  
Acer Saccharum ◽  
Sugar Maple ◽  
Arbuscular Mycorrhizae ◽  
Vascular Plant ◽  
Early Spring ◽  
Mycorrhizal Colonization ◽  
Root Anatomy ◽  
Herbaceous Plants ◽  
Forest Site ◽  
Almost All

The phenology, root anatomy, and mycorrhizal colonization of vascular plant species occurring in a hardwood forest site were studied. Sugar maple (Acer saccharum), the dominant tree, ash (Fraxinus americana), another important tree, and the most abundant herbaceous plants were examined periodically. Most of the herbaceous species present were highly colonized by vesicular–arbuscular mycorrhizae (VAM), but some consistently had low levels of VAM, or were nonmycorrhizal, and several had orchid mycorrhizae. Most trees were associated with VAM, although several ectomycorrhizal species occurred in low numbers. Herbaceous plants could be divided into four phenological categories of shoot activity, spring, spring–summer, summer, and fall, but many species had periods of root growth that did not parallel their aboveground activity. Almost all species that produced new roots in summer were well colonized by VAM, while those active mainly in late fall or early spring tended to be nonmycorrhizal. Roots of the woodland plants typically had a well-developed exodermis and also tended to be long-lived and coarse. Possible relationships among these root system characteristics, phenological categories, and levels of mycorrhizal colonization of woodland plants are discussed.

Extinctions in Mediterranean areas

1994 ◽  
Vol 344 (1307) ◽  
pp. 41-46 ◽  
Keyword(s):  
Plant Species ◽  
Western Australia ◽  
Vascular Plants ◽  
Large Scale ◽  
Vascular Plant ◽  
Extinction Rate ◽  
Species Loss ◽  
Higher Plant ◽  
Threatened Plant ◽  
Extinction Rates

Thirty-one species and two subspecies of vascular plants of the M editerranean area are presumed extinct. This would correspond to an extinction rate of 0.11 % of the native M editerranean flora, which compares with rates of 0.3% for vascular plant species of the Cape floristic province of S. Africa, 0.4% for higher plant taxa of California, and 0.66% for those of Western Australia. Percentages of threatened plant taxa are between 25 and 125 times as high as extinction rates. Records of plant extinctions are both incomplete and error-prone, as shown by examples, but even with improving knowledge the rates of species loss are unlikely to change significantly. They are lowest for the M editerranean area, in which hum an im plantation is most ancient, and for which large-scale undocumented early extinction is assumed, and highest for the most recently colonized area, south-western Australia, where extinction may now be at its peak. At least for the M editerranean, aiming at the rescue of each and every species in danger is a realistic if ambitious goal.

scholarly journals Organic, integrated and conventional management in apple orchards: effect on plant species composition, richness and diversity

2011 ◽  
Vol 59 (5) ◽  
pp. 151-158 ◽  
Author(s):  
Zdeňka Lososová ◽  
Michaela Kolářová ◽  
Luděk Tyšer ◽  
Samuel Lvončík
Keyword(s):  
Species Composition ◽  
Plant Species ◽  
Native Species ◽  
Vascular Plant ◽  
Permanent Plots ◽  
Plant Species Composition ◽  
Apple Orchards ◽  
Higher Plant ◽  
Organic Management ◽  
Spontaneous Vegetation

The study was conducted to assess the effect of conventional, integrated and organic management on differences in plant species composition, richness and diversity. The plants were studied in triads of orchards situated in three regions of the Czech Republic. Data about species occurrences were collected on 15 permanent plots in the tree rows and 15 plots between tree rows in each of the apple orchards during 2009. A total of 201 vascular plant species (127 native species, 65 archaeophytes, and 9 neophytes) were found. Management type and also different regional conditions had a significant effect on plant species composition and on diversity parameters of orchard spontaneous vegetation. Species richness and species pool was significantly higher in the organic orchards than in the differently managed orchards. Management type had significant effect on proportions of archaeophytes, and also neophytes in apple orchards. The results showed that a change from conventional to integrated and organic management in apple orchards lead to higher plant species diversity and to changes in plant species composition.

scholarly journals Menage a trois - are two fungi too much for ryegrass?

2007 ◽  
Vol 13 ◽  
pp. 181-183
Author(s):  
Q. Liu ◽  
A.J. Parsons ◽  
H. Xue ◽  
H. Harzer ◽  
S. Rasmussen
Keyword(s):  
Lolium Perenne ◽  
Glomus Mosseae ◽  
Arbuscular Mycorrhizae ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Available P ◽  
Higher Plant ◽  
Neotyphodium Lolii ◽  
Mycorrhizal Inoculation ◽  
Plant Available P

A pot experiment investigated interactions between foliar endophytic (EP, Neotyphodium lolii) and root arbuscular mycorrhizal (AM, Glomus mosseae) fungi in Lolium perenne. The effects of three phosphorus (P) forms (KH2PO4-P, phosphate rock-P (PR) and phytate-P) on EP and AM colonisation and plant growth parameters were also assessed. AM arbuscular and vesicular structures were estimated by microscopic counting and fungal concentrations were quantified by qPCR of G. mosseae and N. lolii specific genes. Significantly more AM structures were formed in roots of EP-free compared to EP-infected plants, indicating that AM development is suppressed by the presence of foliar EPs. Total AM concentrations were also generally higher in EP-free plant roots, but not significantly. EP concentrations were significantly reduced by higher plant-available P in the growth medium, but mycorrhizal infection did not significant affect EP concentrations. Mycorrhizal inoculation increased shoot dry matter (DM) at low plant-available P in the PR treatment. Infection with the endophytic strain AR37 also significantly increased shoot DM and fungal concentrations were higher compared to CS (common or wild-type strain)- and AR1-infected plants. Insoluble phytate-P produced the same amount of biomass as soluble-P (K-Pi) did, suggesting that L. perenne has the ability to use organic P. Keywords: Neotyphodium lolii, Glomus mosseae, foliar endophyte, arbuscular mycorrhizae, Lolium perenne, qPCR, phosphate, AR1, AR37

The first record of mycorrhizae on sub-Antarctic Heard Island: a preliminary examination

2005 ◽  
Vol 17 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Y. FRENOT ◽  
D.M. BERGSTROM ◽  
J.C. GLOAGUEN ◽  
R. TAVENARD ◽  
D.G. STRULLU
Keyword(s):  
Nutrient Uptake ◽  
Plant Species ◽  
Host Plants ◽  
Arbuscular Mycorrhizae ◽  
Vascular Plant ◽  
First Record ◽  
Vesicular Arbuscular Mycorrhizae ◽  
Preliminary Examination ◽  
Heard Island ◽  
Vesicular Arbuscular

Roots of nine of the 12 vascular plant species present on sub-Antarctic Heard Island were examined for mycorrhizae. All species examined had some type of mycorrhization with most possessing associations with vesicular-arbuscular mycorrhizae or dark septate mycorrhizae. The degree of mycorrhization varied considerably across sites. Sampled plants were growing on either morainic or beach substrates with some areas exposed only in the last century. As mycorrhizae are known to play an important role in the nutrient uptake by host-plants, these results support the idea that mycorrhizae can influence the capacities of plants to colonize in cold and low-nutrient environments such as sub-Antarctic glacier forelands. Comparisons with data from other sub-Antarctic islands are made.

Ovarian Dysfunction in Fetally Irradiated Beagles

1977 ◽  
Vol 35 ◽  
pp. 658-659
Author(s):  
T. M. Seed ◽  
M. H. Sanderson ◽  
D. L. Gutzeit ◽  
T. E. Fritz ◽  
D. V. Tolle ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Low Dose ◽  
Long Term Effects ◽  
Ovarian Dysfunction ◽  
Dose Rates ◽  
Highly Sensitive ◽  
Microscopic Evaluation ◽  
Ovarian Pathology ◽  
Normal Offspring

The developing mammalian fetus is thought to be highly sensitive to ionizing radiation. However, dose, dose-rate relationships are not well established, especially the long term effects of protracted, low-dose exposure. A previous report (1) has indicated that bred beagle bitches exposed to daily doses of 5 to 35 R 60Co gamma rays throughout gestation can produce viable, seemingly normal offspring. Puppies irradiated in utero are distinguishable from controls only by their smaller size, dental abnormalities, and, in adulthood, by their inability to bear young.We report here our preliminary microscopic evaluation of ovarian pathology in young pups continuously irradiated throughout gestation at daily (22 h/day) dose rates of either 0.4, 1.0, 2.5, or 5.0 R/day of gamma rays from an attenuated 60Co source. Pups from non-irradiated bitches served as controls. Experimental animals were evaluated clinically and hematologically (control + 5.0 R/day pups) at regular intervals.

Selection and Preparation of Specific Tissue Regions For Tem Using Large Epoxy-Embedded Blocks

1973 ◽  
Vol 31 ◽  
pp. 324-325 ◽  
Author(s):  
P. M. Lowrie ◽  
W. S. Tyler
Keyword(s):  
Electron Microscopy ◽  
Anatomical Structures ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Embedded Blocks ◽  
Specific Tissue ◽  
Definition Of ◽  
Areas Of Interest ◽  
Selection Of

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).

TEM comparison of osmium vs osmium with potassium ferricyanide secondary fixatives and the impact of secondary fixative temperature on tissue preservation/contrast quality

1996 ◽  
Vol 54 ◽  
pp. 910-911
Author(s):  
J. W. Horn ◽  
B. J. Dovey-Hartman ◽  
V. P. Meador
Keyword(s):  
Osmium Tetroxide ◽  
Potassium Ferricyanide ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Cacodylate Buffer ◽  
Transmission Electron Microscopic ◽  
Glutaraldehyde Solution ◽  
Temperature Impact ◽  
The Impact

Osmium tetroxide (OsO4) is a universally used secondary fixative for routine transmission electron microscopic evaluation of biological specimens. Use of OsO4 results in good ultrastructural preservation and electron density but several factors, such as concentration, length of exposure, and temperature, impact overall results. Potassium ferricyanide, an additive used primarily in combination with OsO4, has mainly been used to enhance the contrast of lipids, glycogen, cell membranes, and membranous organelles. The purpose of this project was to compare the secondary fixative solutions, OsO4 vs. OsO4 with potassium ferricyanide, and secondary fixative temperature for determining which combination gives optimal ultrastructural fixation and enhanced organelle staining/contrast.Fresh rat liver samples were diced to ∼1 mm3 blocks, placed into porous processing capsules/baskets, preserved in buffered 2% formaldehyde/2.5% glutaraldehyde solution, and rinsed with 0.12 M cacodylate buffer (pH 7.2). Tissue processing capsules were separated (3 capsules/secondary fixative.solution) and secondarily fixed (table) for 90 minutes. Tissues were buffer rinsed, dehydrated with ascending concentrations of ethanol solutions, infiltrated, and embedded in epoxy resin.

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