scholarly journals Isolation, Identification, and Antibiotics Susceptibility Determination of Proteus Species Obtained from Various Clinical Specimens in Erbil City

2019 ◽  
Vol 9 (2) ◽  
pp. 86-92
Author(s):  
Treska D. Kamil ◽  
Sanaria F. Jarjes
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Proteus Vulgaris ◽  
Clinical Specimens ◽  
Vaginal Swabs ◽  
Vitek 2 ◽  
Proteus Hauseri ◽  
Antibiotics Susceptibility ◽  
Proteus Species

Fifty-two Proteus isolates, (47) Proteus mirabilis, (4) Proteus vulgaris, and (1) Proteus hauseri are being isolated from (200) clinical specimens taken from patients admitted to different hospitals in Erbil city/Kurdistan region of Iraq. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. All isolates were identified depending on cultural, morphological, biochemical characteristics, and confirmatory VITEK 2 system. Furthermore, VITEK 2 (antibiotic susceptibility testing) panel was used to determine the antibiotic susceptibility of Proteus isolates, and the results showed that all isolates were entirely resistant to tetracycline and tigecycline (100%), but sensitive to meropenem. Furthermore, the present study reported a case of rare Proteus species – P. hauseri – isolated from a patient with urinary tract infection in Erbil City which characterized by no swarming on blood agar.

scholarly journals Antibiotic Susceptibility Profile of Enterobacteriaceae Isolates from a Locally Preferred Drink – Kunun-Zaki in Kaduna State

2021 ◽  
Vol 16 (2) ◽  
pp. 149-157
Author(s):  
S.K. Parom ◽  
G.D. Gamaleon ◽  
S.G. Ishaku ◽  
J.C. Igwe ◽  
O.F. Obajuluwa
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Bacterial Isolates ◽  
Proteus Vulgaris ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Potential Source ◽  
The People ◽  
Antibiotics Susceptibility ◽  
Potential Public Health

Background: ‘Kunun-zaki’ is a local drink made from slightly fermented corn or maize, with water as the medium. It is commonly taken by the people in the northern part of Nigeria. It has high nutritional benefit, even though it can also serve as a potential source of waterborne disease if not prepared under strict hygienic condition.Objective: This study was carried out to isolate Enterobacteriaceae bacteria from ‘kunun-zaki’ drinks and to determine their antibiotic susceptibility to commonly prescribed antibiotics within Kaduna metropolis of Kaduna State, Nigeria.Methods: A total of 20 samples were randomly collected from different locations (within a period of two weeks), analysed and coliform count determined. The bacterial isolates were identified using gram staining, selective media and standard biochemical tests. Antibiotics susceptibility testing was carried out using Kirby-Bauer agar diffusion method.Results: The following bacteria were isolated: Enterobacteriaceae (50%), Staphylococcus aureus (30%), Pseudomonas aeruginosa (20%). The Enterobacteriaceae bacteria isolated were: Klebsiella pneumoniae 4 (20%), Escherichia coli 4, (20%), Citrobacter freundii 1 (5%) and Proteus vulgaris 1 (5%). The antibiotic susceptibility testrevealed that ciprofloxacin, gentamicin, imipenem and ofloxacin (each 100%) chloramphenicol (90%), amoxicillinclavulanate (80%) and streptomycin (70%) were active against the isolates while the isolates were resistant to ceftazidime (100%), meropenem (80%), cefoxitin and ceftriaxone (70%).Conclusion: This study showed the presence of Enterobacteriaceae contaminants in locally consumed drink (kununzaki) sold within Kaduna metropolis which pose a potential public health risk including the risk of transmission of strains which can lead to the spread of antibiotic resistance in the society.

scholarly journals Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs

2015 ◽  
Vol 72 (11) ◽  
pp. 996-1003 ◽  
Author(s):  
Natasa Stankovic-Nedeljkovic ◽  
Branislav Tiodorovic ◽  
Branislava Kocic ◽  
Vojislav Ciric ◽  
Marko Milojkovic ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Susceptibility Testing ◽  
Wound Infections ◽  
Common Cause ◽  
Resistance To Antibiotics ◽  
Mucous Membranes ◽  
Common Serotypes ◽  
Antibiotics Susceptibility

Introduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of wound infections, following the disruption of the skin or mucous membranes integrity. The aim of this study was to analyze of the presence P. aeruginosa in wound swabs, antibiotics susceptibility testing, determination of the minimum inhibitory concentrations (MICs) of antibiotics, testing of the metallo-?-lactamases (MBLs) production, isolates serotyping and analysis of the most common serotypes resistance. Methods. A total of 90 outpatients and 55 intpatients wound swabs were cultivated. Wound swabs were taken from the patients with wound infections symptoms. Antibiotics susceptibility testing was performed to: meropenem, imipenem, piperacillin-tazobactam, ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin, ciprofloxacin and colistin (HiMedia). Polyvalent and monovalent antisera for agglutination (Biorad) were used in P. aeruginosa agglutination. Results. P. aeruginosa was isolated from 36.55% wound swabs (36.66% of the inpatients wounds and 36.36% of the outpatients). The analyzed isolates showed the highest degree of sensitivity to colistin (100%) and meropenem (93.44%) and the lowest to cefepime (19.54%). The majority of the inpatients isolates had 12 ?g/mL (28.57%) MIC for piperacillin-tazobactam and 16 ?g/mL (28.57%) for the outpatients. The most common MICs for ciprofloxacin were 0.19 ?g/mL (31.81%) for the nosocomial isolates, and 0.25 ?g/mL (28.57%) for the outpatients? ones. The most common MICs for amikacin of the nosocomial isolates were 6 ?g/ml (40.9%), and for the outpatients ones 4 ?g/mL (33.33%). Five (9.43%) isolates produced MBLs. The most common serotypes were P11 (22.64%), P6 (15.09%) and P1 (11.32%). Conclusion. Neither the increased presence of P. aeruginosa was noticed in wounds swabs, nor the antibiotic resistance in the nosocomial isolates compared to those from outpatients. The analyzed isolates had the higest sensitivity to colistin and meropenem, and the lowest to cefepime.

scholarly journals A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system

2013 ◽  
Vol 62 (5) ◽  
pp. 773-777 ◽  
Author(s):  
Guy Prod’hom ◽  
Christian Durussel ◽  
Gilbert Greub
Keyword(s):  
Ammonium Chloride ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Correct Identification ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Identification ◽  
Lower Accuracy ◽  
Direct Inoculation ◽  
Vitek 2

An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99 % for Enterobacteriaceae and 74 % for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3 % for Enterobacteriaceae, and 0.7 and 0.1 % for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.

scholarly journals Prevalence and antibiotic susceptibility of ear pathogens isolated from patients in Tripoli, north of Lebanon

10.3823/0801 ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Monzer Hamze ◽  
Marwan Osman ◽  
Hassan Mallat ◽  
Marcel Achkar
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Public Health Problem ◽  
Epidemiological Data ◽  
Resistance Level ◽  
Antibiotic Susceptibility Patterns ◽  
Culture Identification ◽  
Antibiotics Susceptibility ◽  
Susceptibility Patterns

Background. Urinary tract infection (UTI) is a severe public health problem. However, infected patients are usually treated empirically without preceding culture or antibiotics susceptibility testing, which may increase the antibiotic resistance level. The aim of this study is to determine the prevalence and antibiotic susceptibility patterns of common bacterial uropathogens isolated in Akkar governorate, North Lebanon. Methods. Spot midstream from urine samples from 9662 patients presenting UTI symptoms who came to Youssef Hospital Center located in Akkar governorate, were collected in sterile plastic cups. Culture, identification and antibiotic susceptibility testing were performed through conventional tools according to the manufacturer’s recommended procedures and the recommendations of the European Committee on Antimicrobial Susceptibility Testing. Results. Overall, a total of 1009 bacterial uropathogens were isolated. Escherichia coli was predominant and represented 72.5% of all isolates , followed by Klebsiella pneumoniae (8.2%), Enterococcus spp. (5.5%), Pseudomonas aeruginosa (4.5%), Proteus spp. (3%), Enterobacter spp. (2%), Staphylococcus aureus (2%), Streptococcus agalactiae (1.6%), Staphylococcus saprophyticus (0.4%), Acinetobacter baumannii (0.2%) and Providencia rettgeri (0.1%). Moreover, the mean antibiotic resistance rates of isolates was relatively high, but similar to previous investigations reported in our country. Conclusion. To our knowledge, this is the first investigation reporting epidemiological data regarding the prevalence and antibiotic susceptibility patterns of uropathogens isolated from patients in Akkar governorate. Our data indicated the urgent need of a strategic plan to tackle antibiotic resistance, particularly in deprived regions with poor healthcare structures such as Akkar governorate. DOI: http://dx.doi.org/10.3823/801

scholarly journals DIAGNOSTIC ACCURACY OF IN-HOUSE BIOCHEMICAL TESTING FOR IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMENSAGAINST VITEK 2 SYSTEM

2021 ◽  
Vol 71 (1) ◽  
pp. 155-61
Author(s):  
Mariam Sarwar ◽  
Syed Adeel Hussain Gardezi ◽  
Gohar Zaman ◽  
Aamer Ikram ◽  
Wajid Hussain ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Antimicrobial Susceptibility ◽  
Gold Standard ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Enterococcus Species ◽  
Clinical Specimens ◽  
Enterococcal Species ◽  
Vitek 2 ◽  
Biochemical Testing

Objective: To determine the diagnostic accuracy of in-house biochemical testing for identification of enterococcus species isolated from various clinical specimens against gold standard i.e., automated Vitek 2 system. This study also includes the antimicrobial susceptibility testing of enterococci against various antimicrobials. Study Design: Cross-sectional comparative study. Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi,from Apr 2017 to Mar 2018. Methodology: A total of 218 isolates from various clinical specimens suspected to be Enterococcus spp. werechecked by in-house biochemical testing including bile esculin, 6.5% NaCl and 1% arabinose and results werecompared with Vitek 2 compact system. The frequencies were determined by both systems and antimicrobial susceptibility testing was performed by disk diffusion as per clinical and laboratory standards institute guidelines. Results: Comparing the results of in-house testing with gold standard i.e., Vitek 2 system, the statistical data was calculated. Sensitivity turned out to be 100%, Specificity was found to be 68.75%. Positive and negative predictive values were 97.58% and 100% respectively. Accuracy turned out to be 97.71%. Conclusion: The in-house biochemical testing can be quite a useful method for identification of enterococci inresource-limited settings. However, it requires overight incubation and cannot identify other enterococcal species and non-enterococcal species. Vitek 2 is an automated system that is easy-to-handle, provides a rapid and reasonably accurate identification of enterococci alongwith accurate AST results. Enterococcal isolates from various clinical specimens in our setup showed least resistance to linezolid, followed by teicoplanin and vancomycin. Nitrofurantoin and.............

Sign in / Sign up

Export Citation Format

Share Document