scholarly journals Biological features and conditions of the surface cultivation of a strain-producer of microbiopreparation 11-3 Bacillus sp. – an antagonist of fusarium pathogens infecting oil flax

2020 ◽  
Vol 4 (184) ◽  
pp. 57-62
Author(s):  
L.V. Maslienko ◽  
◽  
A.Kh. Voronkova ◽  
Keyword(s):  
Bacterial Strain ◽  
Nutrient Medium ◽  
Nitrogen Nutrition ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Biological Features ◽  
Wettable Powder ◽  
Optimal Period ◽  
Fusarium Pathogens ◽  
Carbon Nutrition

To develop a technological regimen of production of microbiopreparation in a preparative form ‘wettable powder’ (WP) we studied biological features and conditions of surface cultivation of the strainproducer 11-3 Bacillus sp. – an antagonist of fusariosis pathogen infecting oil flax. Cultural qualities of the strain-producer were studied in three agarized mediums. The optimal conditions for cultivation of the strain-producer on liquid nutrient mediums are estimated: temperature – 30–35 оС, рН – 8–10. As a source of carbon nutrition, glycerin and molasses can be used. Yeastrel and corn-steep concentrate are the optimal sources of nitrogen nutrition; the Tylon’s medium is optimal compound liquid nutrient medium for cultivation of the perspective bacterial strain 11-3 Bacillus sp. Optimal period of the surface cultivation of the bacterial strain 11-3 Bacillus sp. on the liquid nutrient Tylon’s medium was ten days, and a volume of sowing culture to nutrient medium – 2.0%.

scholarly journals THE BIOLOGICAL PECULIARITIES OF PRODUCER STRAIN OF MICROBIOPREPARATION 11-1 BACILLUS SP. – THE ANATAGONIST OF SUNFLOWER PHOMA ROT PATHOGEN

2021 ◽  
Author(s):  
E.A. Efimtseva ◽  
Keyword(s):  
Nutrient Medium ◽  
Nitrogen Nutrition ◽  
Maximum Diameter ◽  
Bacillus Sp ◽  
Producer Strain ◽  
Wettable Powder ◽  
Agar Media ◽  
Carbon Nutrition ◽  
Complex Liquid ◽  
Potato Sucrose Agar

We studied the cultural and physiological characteristics of the producer strain 11-1 Bacillus sp. – the antagonist of sunflower Phoma rot pathogen to develop the technological regulations for the production of a microbiological preparation in a «wettable powder» form. We studied the cultural characteristics of the producer strain on three agar media: potato sucrose agar (PSA), Czapek’s agar and Tylon-3; the characteristics varied significantly depending on the nutrient medium. The maximum diameter of colonies on the tenth day of incubation developed on the Tylon-3 medium – 66×99 mm. We established the optimal conditions for the cultivation of the strain on liquid nutrient media: temperature – 30–35 °С, medium pH from 6 to 10. We found that molasses is an optimal source of carbon nutrition, while peptone is the most favorable source of nitrogen nutrition. We established that the Tylon-3 medium is the optimal complex liquid nutrient medium for the cultivation of the bacterial strain 11-1 Bacillus sp.

scholarly journals Biological features and conditions of surface cultivating of a strain-producer of microbiopreparation Т-1 Trichoderma sp. against pathogen causing fusarium on oil flax

2020 ◽  
Vol 3 (183) ◽  
pp. 121-128
Author(s):  
L.V. Maslienko ◽  
◽  
A.Kh. Voronkova ◽  
Keyword(s):  
Nutrient Medium ◽  
Dry Mass ◽  
Fungal Strain ◽  
Spore Formation ◽  
Maximal Growth ◽  
Mycelium Growth ◽  
Biological Features ◽  
Trichoderma Sp ◽  
Optimal Period ◽  
Fungus Mycelium

To develop technological regimen for production of microbiopreparations in a preparation form ‘wetting powder’ we studied biological features and conditions of surface cultivating of a strain-producer Т-1 Trichoderma sp. – an antagonist of pathogen Fusarium oxysporum Schlecht. emend. Shyd. et Hans. var. orthoceras (App. еt Wr.) Bilai and Fusarium poae (Peck) Wollenw., Lewis on oil flax. To study cultural and physiological qualities of the strainproducer we used agar and liquid mediums. Surface cultivation of a fungus on agar and liquid Rudakov’s medium at a temperature 25–30 оС was the most favorable for mycelium growth and spore formation. Stationary fungus cultivation on liquid medium with рН from 3 to 6 provided maximal mycelium growth with spore formation and the highest dry mass. Addition of starch into the Chapek’s nutrient medium caused maximal growth of fungus mycelium and increase of its dry mass. The best source of nitrogen for a fungus strain was corn extract. Rudakov’s and No1 mediums are optimal compound liquid nutrient mediums for a surface cultivation of the strain-producer. Optimal period of the surface cultivation of the fungal strain Т-1 Trichoderma sp. on liquid Rudakov’s nutrient medium was 10 days, and a volume of sowing culture to a nutrient medium – 2.0%.

scholarly journals Melanin synthesis by black yeast-like fungi Psedonadsoniella brunnea: dependence of L-tyrosine quantity in the cultural medium

2019 ◽  
Vol 26 (1) ◽  
pp. 41-46 ◽  
Author(s):  
T. Kondratiuk ◽  
T. Beregova ◽  
T. Akulenko ◽  
Ie. Torgalo ◽  
V. Vereschaka
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Statistical Processing ◽  
Black Yeast ◽  
Malt Extract ◽  
Melanin Synthesis ◽  
Optimal Conditions ◽  
Cultural Medium ◽  
Yeast Fungi ◽  
Series Of Experiments

To determine the optimal conditions for the synthesis of melanin by black yeast fungi Pseudonadsoniella brunnea (Basidiomycota, Agaricomycotina, Agaricomycetes, Polyporales, Meripilaceae), depending on the amount of L-tyrosine in the culture medium was the purpose of the work. The standard Malt Extract Broth (MEB) liquid nutrient medium was used within this study. L-tyrosine was added to the culture medium in a quantity of 0.01, 0.025 and 0.05%.To obtain the melanin the cultivation of Pseudonadsoniella brunnea was carried out at pH 1-1.5, temperature + 21 ± 1 ° C during 7 days. Statistical processing of the results was carried out using generally accepted methods of variation statistics. It has been established that the level of melanin synthesis by black yeast-like fungi Pseudonadsoniella brunnea depends on the amount of L-tyrosine introduced into the culture medium. The MEB nutrient medium containing 0.05% L-tyrosine in this series of experiments found to be the best composition for obtaining melanin by the strain-producer Pseudonadsoniella brunnea. Compared to control (MEB without L-tyrosine), the amount of melanin synthesized by Ps. brunnea in these conditions increased by 2.5 times. The further research into the optimal conditions for the cultivation of black yeast-like fungi Pseudonadsoniella brunnea in order to obtain melanin is relevant and promising.

scholarly journals Dynamics of ibuprofen biodegradation by Bacillus sp. B1(2015b)

2017 ◽  
Vol 43 (2) ◽  
pp. 60-64 ◽  
Author(s):  
Ariel Marchlewicz ◽  
Urszula Guzik ◽  
Danuta Wojcieszyńska
Keyword(s):  
Bacillus Thuringiensis ◽  
Carbon Source ◽  
Surface Waters ◽  
Bacterial Strain ◽  
Harmful Effect ◽  
Bacillus Sp ◽  
High Intake ◽  
Over The Counter ◽  
New Methods ◽  
Inflammatory Drugs

Abstract High intake of over-the-counter, non-steroidal anti-inflammatory drugs, such as ibuprofen, has resulted in their presence in wastewaters and surface waters. The potentially harmful effect of ibuprofen present in the waters has led to a search for new methods of drugs’ removal from the environment. One of the most important technological and economical solutions comprises microbiological degradation of these resistant pollutants. Searching for new strains able to degrade ibuprofen could be one of the answers for increasing the detection of pharmaceuticals in the waters. In this study, the ability of bacterial strain Bacillus thuringiensis B1(2015b) to remove ibuprofen is described. Bacteria were cultured in both monosubstrate and cometabolic systems with 1, 3, 5, 7 and 9 mg L-1 ibuprofen and 1 g L-1 glucose as a carbon source. Bacillus thuringiensis B1(2015b) removed ibuprofen up to 9 mg L-1 in 232 hours in the monosubstrate culture, whereas in the cometabolic culture the removal of the drug was over 6 times faster. That is why the examined strain could be used to enhance the bioremediation of ibuprofen.

scholarly journals Isolation of Bacillus Strains with Keratinolitic Activity

2020 ◽  
Author(s):  
S. Aktayeva ◽  
K. Baltin ◽  
B. Khassenov
Keyword(s):  
Proteolytic Enzymes ◽  
Sole Source ◽  
Environmental Safety ◽  
Economic Feasibility ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Processing Waste ◽  
Keratinolytic Activity ◽  
Chicken Feathers ◽  
Promising Source

Environmental safety and economic feasibility determine the search for new ways of processing waste in poultry farms. Most of this waste is down and feathers, which are 90% β-keratin. Feathers can be a valuable source of amino acids and peptones when properly processed. The most effective is enzymatic treatment of feather keratin. The search for new strains producing keratinolytic enzymes seems to be a promising direction. On the territory of the poultry farm, 4 strains were isolated from the places of accumulation of feathers. They are able to use chicken feathers as their sole source of organic matter. Based on morphological, genomic, and proteomic analyzes, the isolated strains were identified as Bacillus sp. It was found that the strains secrete proteolytic enzymes that hydrolyze collagen, casein, β-keratin and do not hydrolyze bovine serum albumin. Feather hydrolysis experiments showed that the Bacillus sp. A5.3 possesses maximum keratinolytic activity, and on the second day, the destruction of the second order barbs is observed. The keratinase activity of the strain on azokeratin after an hour of incubation on feather medium was 27.4 U/ml. The optimal conditions for the complex of secreted proteolytic enzymes are pH 7.0-8.0 and temperature 35-40 °C. The isolated Bacillus sp. A5.3 strain is a promising source of proteases and keratinases.

scholarly journals Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

2021 ◽  
Vol 209 (06) ◽  
pp. 43-52
Author(s):  
Marina Markova ◽  
Elena Somova
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Optimal Time ◽  
In Vitro Cultivation ◽  
Initiation Time ◽  
Multiplication Factor ◽  
Optimal Conditions ◽  
Prunus Domestica ◽  
Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

Étude de quelques aspects du métabolisme carboné et azoté chez l'Arceuthobium oxycedri, gui nain du genévrier

1992 ◽  
Vol 70 (8) ◽  
pp. 1709-1716
Author(s):  
L. Rey ◽  
A. Sadik ◽  
A. Fer ◽  
S. Renaudin
Keyword(s):  
Amino Acids ◽  
Photosynthetic Activity ◽  
Nitrogen Nutrition ◽  
Nitrogen Supply ◽  
Dwarf Mistletoe ◽  
Gas Exchanges ◽  
Nitrogenous Compounds ◽  
Slow Development ◽  
Carbon Nutrition ◽  
Glutamate Oxalacetate Transaminase

In juniper dwarf mistletoe, Arceuthobium oxycedri (DC) M. Bieb., a hemiparasitic and epiphytic Angiosperm characterized by very reduced and slow development, the chlorophyll content of the shoots is relatively high for a parasitic plant. However, gas exchanges reveal a low net gain in carbon, and the real photosynthetic intensity is only about 1.5 to 2 times higher than the respiratory intensity in these organs. In the endophytic system, which is also chlorophyllous, a small amount of photosynthetic activity also occurs. By studying 14CO2 assimilation and carboxylase activities in both organs, it was possible to determine the ability of the parasite to satisfy part of its carbon requirements. Following the administration of 14CO2, radioactivity was mainly recovered in carbohydrates and to a lesser extent in organic acids and amino acids. This demonstrates that A. oxycedri has some autonomy for the fixation and distribution of carbon into various compounds. On the other hand, the parasite, since it is epiphytic, is completely dependent upon its host for nitrogen. The study of the activities of the key enzymes of nitrogen metabolism ([Formula: see text] and [Formula: see text] reductases, glutamine synthetase, glutamate dehydrogenase, glutamate-oxalacetate transaminase) shows that nitrogen supply from the host certainly occurs mainly in the form of reduced nitrogenous compounds (ammonia and (or) aminated organic compounds). The numerous labelled amino acids recovered after administration of 14CO2 show that A. oxycedri is able to redistribute the imported nitrogen in various ways. Key words: Arceuthobium oxycedri, parasitic Angiosperms, carbon nutrition, nitrogen nutrition.

scholarly journals Agrobiological evaluation of new apple clonal rootstocks of the Michurinsk State Agrarian University selection using different breeding methods

2020 ◽  
Vol 23 ◽  
pp. 01002
Author(s):  
Zinaida N. Tarova ◽  
Nataliya L. Churikova ◽  
Maxim L. Dubrovsky ◽  
Andrey V. Kruzhkov ◽  
Natalya N. Savelyeva
Keyword(s):  
Nutrient Medium ◽  
Apple Tree ◽  
Breeding Methods ◽  
Apple Trees ◽  
Biological Features ◽  
Clonal Micropropagation ◽  
Time Treatment ◽  
Yield And Quality ◽  
University Selection

The results of many years of research on propagation of promising forms of clonal stocks of apple trees of the Michurinsk State Agrarian University selection in the mother plantation by stooling, by herbaceous cuttings and by clonal micropropagation are presented. According to the complex of economic and biological features, promising clonal rootstocks of an apple tree with high shoot-forming capacity in the mother plant were identified: dwarf apple trees 2-3-49, 2-15-2, 2-15-15, 3-4-7, 5-21-27; semi dwarf apple trees 2-3-14, 2-9-49, 2-9-102, 2-12-10, 5-24-1, 5-27-1. The rootstocks that are promising for propagation by herbaceous cuttings – 2-3-14, 2-3-49, 2-12-10, 2-15-15, 3-4-7, 5-21-93; at the same time, treatment with exogenous promoters such as IBA and NAA at a concentration of 50 mg/l increases the yield and quality of stock material. A modified MSO nutrient medium with the addition of 0.5-1.0 mg/l of 6-BAP and 0.1-0.2 mg/l of IBA is selected for the clonal micropropagation of apple stocks.

scholarly journals Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains

2018 ◽  
Vol 8 (10) ◽  
pp. 1855 ◽  
Author(s):  
Josef Trögl ◽  
Catherine Esuola ◽  
Sylvie Kříženecká ◽  
Pavel Kuráň ◽  
Lenka Seidlová ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Petroleum Refinery ◽  
Rhodococcus Erythropolis ◽  
Dry Weight ◽  
Aliphatic Hydrocarbons ◽  
Bacillus Sp ◽  
Polluted Soils ◽  
Great Demand ◽  
High Concentrations ◽  
Petroleum Pollutants

At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils.

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