scholarly journals THE BIOLOGICAL PECULIARITIES OF PRODUCER STRAIN OF MICROBIOPREPARATION 11-1 BACILLUS SP. – THE ANATAGONIST OF SUNFLOWER PHOMA ROT PATHOGEN

2021 ◽  
Author(s):  
E.A. Efimtseva ◽  
Keyword(s):  
Nutrient Medium ◽  
Nitrogen Nutrition ◽  
Maximum Diameter ◽  
Bacillus Sp ◽  
Producer Strain ◽  
Wettable Powder ◽  
Agar Media ◽  
Carbon Nutrition ◽  
Complex Liquid ◽  
Potato Sucrose Agar

We studied the cultural and physiological characteristics of the producer strain 11-1 Bacillus sp. – the antagonist of sunflower Phoma rot pathogen to develop the technological regulations for the production of a microbiological preparation in a «wettable powder» form. We studied the cultural characteristics of the producer strain on three agar media: potato sucrose agar (PSA), Czapek’s agar and Tylon-3; the characteristics varied significantly depending on the nutrient medium. The maximum diameter of colonies on the tenth day of incubation developed on the Tylon-3 medium – 66×99 mm. We established the optimal conditions for the cultivation of the strain on liquid nutrient media: temperature – 30–35 °С, medium pH from 6 to 10. We found that molasses is an optimal source of carbon nutrition, while peptone is the most favorable source of nitrogen nutrition. We established that the Tylon-3 medium is the optimal complex liquid nutrient medium for the cultivation of the bacterial strain 11-1 Bacillus sp.

scholarly journals Biological features and conditions of the surface cultivation of a strain-producer of microbiopreparation 11-3 Bacillus sp. – an antagonist of fusarium pathogens infecting oil flax

2020 ◽  
Vol 4 (184) ◽  
pp. 57-62
Author(s):  
L.V. Maslienko ◽  
◽  
A.Kh. Voronkova ◽  
Keyword(s):  
Bacterial Strain ◽  
Nutrient Medium ◽  
Nitrogen Nutrition ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Biological Features ◽  
Wettable Powder ◽  
Optimal Period ◽  
Fusarium Pathogens ◽  
Carbon Nutrition

To develop a technological regimen of production of microbiopreparation in a preparative form ‘wettable powder’ (WP) we studied biological features and conditions of surface cultivation of the strainproducer 11-3 Bacillus sp. – an antagonist of fusariosis pathogen infecting oil flax. Cultural qualities of the strain-producer were studied in three agarized mediums. The optimal conditions for cultivation of the strain-producer on liquid nutrient mediums are estimated: temperature – 30–35 оС, рН – 8–10. As a source of carbon nutrition, glycerin and molasses can be used. Yeastrel and corn-steep concentrate are the optimal sources of nitrogen nutrition; the Tylon’s medium is optimal compound liquid nutrient medium for cultivation of the perspective bacterial strain 11-3 Bacillus sp. Optimal period of the surface cultivation of the bacterial strain 11-3 Bacillus sp. on the liquid nutrient Tylon’s medium was ten days, and a volume of sowing culture to nutrient medium – 2.0%.

scholarly journals THE PRODUCTION OF SELECTIVE ADDITIVES TO GROWING MEDIUM DRIGALSKI LACTOSE AGAR

2019 ◽  
pp. 95-102
Author(s):  
Владимир Ермаков ◽  
Vlyadimir Ermakov ◽  
Оксана Датченко ◽  
Oksana Datchenko ◽  
Юлия Курлыкова ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
First Generation ◽  
Animal Species ◽  
Agar Medium ◽  
Nutrient Media ◽  
Gram Negative ◽  
Selective Media ◽  
Growing Medium ◽  
Agar Media ◽  
Growth And Reproduction

The purpose of the study is to improve the selective supplement for selective media with the purpose to produce enterobacteria. Tasks of the study are to identify the sensitivity of strains obtained of enterobacteria in regard to an-tibiotics; develop a new selective supplement with antibiotics to the nutrient medium Drigalski Lactose Agar. Media should have a content that in the best way possible ensures the growth and reproduction of microorganisms of cer-tain species or family. Intensive biotechnology development and Microbiology allows today to develop new nutrient media and modify the already existing content of media. The object of the study was a new selective additive with antibiotics to the nutrient medium Drigalski Lactose Agar. 253 isolates of bacteria produced from the intestinal mi-crobiotope of different animal species have been the Material for research. The study was conducted in the period from 2010 to 2017. Carbenicillin 30±2.3 from the group of carboxypenicillins and piperacillin 37±2.5 from the group of ureidopenicillins, kanamycin 24±1.5, amikacin 26±1.7 and gentamicin 25±0,8, cefepime 38±3.2 from the group of IV generation cephalosporins, tetracycline 28±1.6, doxycycline 34±2.3 and chloramphenicol 31±2.5, nalidixic acid 37±2.8, trimethoprim 35±3,4 demonstrated the greatest antimicrobial activity against all cultures of enterobac-teria that has been achieved. The high resistance of enterobacteria was shown to benzylpenicillin from the group of natural penicillins, to streptomycin, cephalotine from the group of cephalosporins of the first generation, to polymyx-in B, to ofloxacin (tarivid) and metronidazole. Antibacterial drugs effective against the accompanying gram-positive and gram-negative microflora were considered as the samples of the selective components. Vancomycin from the group of glycopeptides, linezolid from the group of oxazolidinones, and telithromycin from the group of ketolides were chosen. Antibiotics vancomycin and telithromycin in a dose of 0.008 g/dm3, linezolid 0.004 g/dm3 were cho-sen as the selective additive to Drigalski Lactose Agar medium.

Étude de quelques aspects du métabolisme carboné et azoté chez l'Arceuthobium oxycedri, gui nain du genévrier

1992 ◽  
Vol 70 (8) ◽  
pp. 1709-1716
Author(s):  
L. Rey ◽  
A. Sadik ◽  
A. Fer ◽  
S. Renaudin
Keyword(s):  
Amino Acids ◽  
Photosynthetic Activity ◽  
Nitrogen Nutrition ◽  
Nitrogen Supply ◽  
Dwarf Mistletoe ◽  
Gas Exchanges ◽  
Nitrogenous Compounds ◽  
Slow Development ◽  
Carbon Nutrition ◽  
Glutamate Oxalacetate Transaminase

In juniper dwarf mistletoe, Arceuthobium oxycedri (DC) M. Bieb., a hemiparasitic and epiphytic Angiosperm characterized by very reduced and slow development, the chlorophyll content of the shoots is relatively high for a parasitic plant. However, gas exchanges reveal a low net gain in carbon, and the real photosynthetic intensity is only about 1.5 to 2 times higher than the respiratory intensity in these organs. In the endophytic system, which is also chlorophyllous, a small amount of photosynthetic activity also occurs. By studying 14CO2 assimilation and carboxylase activities in both organs, it was possible to determine the ability of the parasite to satisfy part of its carbon requirements. Following the administration of 14CO2, radioactivity was mainly recovered in carbohydrates and to a lesser extent in organic acids and amino acids. This demonstrates that A. oxycedri has some autonomy for the fixation and distribution of carbon into various compounds. On the other hand, the parasite, since it is epiphytic, is completely dependent upon its host for nitrogen. The study of the activities of the key enzymes of nitrogen metabolism ([Formula: see text] and [Formula: see text] reductases, glutamine synthetase, glutamate dehydrogenase, glutamate-oxalacetate transaminase) shows that nitrogen supply from the host certainly occurs mainly in the form of reduced nitrogenous compounds (ammonia and (or) aminated organic compounds). The numerous labelled amino acids recovered after administration of 14CO2 show that A. oxycedri is able to redistribute the imported nitrogen in various ways. Key words: Arceuthobium oxycedri, parasitic Angiosperms, carbon nutrition, nitrogen nutrition.

scholarly journals Stability of IAA and IBA in Nutrient Medium to Several Tissue Culture Procedures

HortScience ◽  
1990 ◽  
Vol 25 (7) ◽  
pp. 800-802 ◽  
Author(s):  
Scott J. Nissen ◽  
Ellen G. Sutter
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Activated Charcoal ◽  
Nutrient Medium ◽  
Relative Stabilities ◽  
Murashige And Skoog Medium ◽  
Agar Media ◽  
Indole 3 Acetic Acid

The relative stabilities of IAA and IBA under various tissue culture procedures were determined. IBA was significantly more stable than IAA to autoclaving. IBA was also found to be more stable than IAA in liquid Murashige and Skoog medium (MS) under growth chamber conditions. The stabilities of IBA and IAA were similar in agar-solidified MS. Light provided by cool-white fluorescent bulbs promoted degradation of IAA and IBA in both liquid and agar media. Activated charcoal in concentrations as high as 5% was found to adsorb more than 97% of IAA and IBA in liquid MS. These results have important implications for the preparation, storage, and handling of IBA and IAA in plant tissue culture. Chemical names used: indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

scholarly journals Biodegradation of textile dyes by bacteria isolated from textile industry effluents

2020 ◽  
Vol 9 (1) ◽  
pp. 5-8
Author(s):  
Khusbu Akter ◽  
Tamanna Zerin ◽  
Avijit Banik
Keyword(s):  
Staphylococcus Aureus ◽  
Textile Industry ◽  
Biochemical Analysis ◽  
Synthetic Dyes ◽  
Bacillus Sp ◽  
Pollution Problem ◽  
Agar Media ◽  
Textile Industries ◽  
Living Organisms ◽  
And Staphylococcus Aureus

A country like Bangladesh where textile industries are the main source of developing economy, pollution problem from such industries creates a huge risk for the environment. Textile industries discharge a huge amount of effluent containing various harmful chemicals including synthetic dyes that are very stable and threat to the living organisms. This study deals with the potential decolorization and biodegradation of Bemacron Yellow HP-2R (BY), Bemacron Red RS (BR) and Bemacron blue RS 01 (BB) dyes using bacteria isolated from textile effluent. The effluent and soil samples were collected from different locations of discharge point. Only two isolates were screened out after primary screening using dye supplemented nutrient agar media. Following colony morphology, physiology and biochemical analysis, they were presumptively identified as Bacillus sp. and Staphylococcus aureus. They were subjected to decolorization of 0.002 g/l BY, BR and BB dyes. Bacillus sp. showed superior decolorization potential of BR (71%) and BB (83%) dyes after 5 days of incubation. Whereas, Staphylococcus aureus showed 79% decolorization of BY dye after 5 days incubation. Decolorization efficacy can be further improved by optimizing environmental conditions and process parameters. Stamford Journal of Microbiology, Vol.9(1) 2019: 5-8

scholarly journals Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains

2018 ◽  
Vol 8 (10) ◽  
pp. 1855 ◽  
Author(s):  
Josef Trögl ◽  
Catherine Esuola ◽  
Sylvie Kříženecká ◽  
Pavel Kuráň ◽  
Lenka Seidlová ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Petroleum Refinery ◽  
Rhodococcus Erythropolis ◽  
Dry Weight ◽  
Aliphatic Hydrocarbons ◽  
Bacillus Sp ◽  
Polluted Soils ◽  
Great Demand ◽  
High Concentrations ◽  
Petroleum Pollutants

At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils.

scholarly journals Uji Resistensi Bakteri terhadap Arsen yang di Isolasi dari Sedimen di Muara Sungai Totok

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Tessalonicha J. Rompis ◽  
Widdhi Bodhi ◽  
Fona Budiarso
Keyword(s):  
Environmental Pollution ◽  
Human Activities ◽  
Toxic Substance ◽  
Mining Industry ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Biochemical Tests ◽  
Soil Sediment ◽  
Agar Media ◽  
Arsenic Resistant Bacteria

Abstract: Arsenic is still used in lots of human activities that results in environmental pollution. Arsenic is also found in nature in a small quantity. Gold mining industry is one of the human activities that uses arsenic. Mining waste that is not managed properly can inflict environmental pollution and harms the human health due to its toxic-substance content. This study was aimed to isolate and identify bacteria in the soil sediment obtained from the estuary area of Totok River, Southeast Minahasa and tested the bacteria's resistance levels to arsenic. Bacteria were isolated from sediment samples from the estuary area of Totok River, and the isolated bacteria were grown in agar media containing arsenic trioxide. Bacteria identification were perfomed by using morphological, physiological and biochemical tests. The results showed that bacteria grew successfully in 10 isolates; 2 isolates were Gram negative bacteria and 8 isolates were Gram positives bacteria. All isolates showed no motility. Identification of bacteria revealed four genera: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp that were resistant to arsenic 1000 ppm. Conclusion: There were 4 genera of arsenic-resistant bacteria identified in the sediment of the estuary area of Totok River, as follows: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp. All of them were resistant to arsenic 1000 ppm.Keywords: sediment, arsenic-resistant bacteria Abstrak: Arsen (As) masih banyak ditemukan dalam kegiatan manusia yang berakibat pencemaran terhadap lingkungan. Arsen juga dapat ditemukan di alam secara alami namun dalam jumlah yang sedikit. Industri pertambangan emas merupakan salah satu kegiatan manusia yang menggunakan arsen. Limbah pertambangan yang tidak dikelola dengan baik dapat mencemari lingkungan dan mengganggu kesehatan manusia karena berpotensi mengandung racun yang berbahaya. Penelitian ini bertujuan untuk mengidentifikasi bakteri resisten arsen serta tingkat resistensinya yang terdapat pada sedimen di muara Sungai Totok Kecamatan Ratatotok Kabupaten Minahasa Tenggara Sulawesi Utara. Bakteri diisolasi dari sedimen di muara sungai Totok dan ditumbuhkan pada media agar yang mengandung arsen trioksida. Identifikasi dari bakteri yang tumbuh menggunakan uji fisiologi, uji morfologi dan uji biokimia. Hasil penelitian mendapatkan 10 isolat dengan bakteri yang berhasil tumbuh, yaitu 2 isolat bakteri Gram negatif dan 8 isolat bakteri Gram positif. Semua isolat tidak menunjukkan adanya motilitas dan dapat dikategorikan ke dalam empat kelompok genus yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Keempat genus bakteri ini resisten terhadap arsen hingga konsentrasi 1000 ppm. Simpulan: Terdapat empat genus bakteri resisten arsen yang teridentifikasi dalam sedimen di muara Sungai Totok, yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Kesemuanya resisten terhadap arsen hingga konsentrasi 1000 ppm.Kata kunci: sedimen, bakteri resisten arsen

THE CARBON AND NITROGEN NUTRITION OF CERTAIN FUNGI

1967 ◽  
Vol 13 (8) ◽  
pp. 1097-1106 ◽  
Author(s):  
Hasan M. Yusef ◽  
Magid E. Allam
Keyword(s):  
Sodium Nitrite ◽  
Sodium Acetate ◽  
Organic Nitrogen ◽  
Sodium Citrate ◽  
Nitrogen Nutrition ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Carbon And Nitrogen ◽  
Carbon Nutrition ◽  
Nigrospora Oryzae

Carbon nutrition of Chaetomium sp., Myrothecium verrucaria, Pestalotia gracilis, and Pleurotus ostreatus was studied. Nitrogen nutrition was also examined with Nigrospora oryzae included. The effect of C/N ratio on fruiting was measured for the first four fungi. Several carbon sources including alcohols, carbohydrates, and organic acids were tested; in general, dextrin and L-arabinose were the most favorable for growth, starch was less so, whereas sodium acetate and sodium citrate were the least favorable. Maltose supported good sporulation of M. verrucaria, P. gracilis, and P. ostreatus. Organic nitrogen was superior to inorganic nitrogen for growth of M. verrucaria, N. oryzae, and P. ostreatus. The opposite was noticed for Chaetomium sp. and P. gracilis. Not all could grow on sodium nitrite at the concentration used nor sporulate on DL-methionine or ammonium sulfate. The best sporulation of P. gracilis was obtained with the maximum glucose and nitrate concentrations used (4% and 1% respectively), whereas the minimum concentrations (0.1 and 0.05% respectively) were best for the sporulation of P. ostreatus. Chaetomium sp. and M. verrucaria fruited best on intermediate concentrations of glucose and nitrate.

scholarly journals Testing of laboratory samples of microbiological preparations based on promising producer strains for the control of apple scab

2021 ◽  
Vol 34 ◽  
pp. 04007
Author(s):  
Lyubov Maslienko ◽  
Galina Yakuba
Keyword(s):  
Venturia Inaequalis ◽  
Apple Scab ◽  
Biological Method ◽  
Producer Strain ◽  
Laboratory Sample ◽  
Scab Control ◽  
Protection Systems ◽  
Wettable Powder ◽  
Krasnodar Region ◽  
Producer Strains

To prevent phytosanitary destabilization in apple agrocenoses, it is necessary to include ecologically safe microbiological preparations in the protection systems. In the Krasnodar region in 2016 and 2019 in the field, laboratory samples of microbiological preparations were tested in the formulation “wettable powder” from the collection of the laboratory of the biological method of the FGBNU VNIIMK against Venturia inaequalis (Cooke) G. Winter. Of the 9 laboratory samples tested in 2016, the highest efficiency, approaching the effectiveness of a chemical fungicide, was shown by samples based on fungal producer strains: RK-1, XK-1 and T-2 - 76.9-92.5%. In 2019, all variants (in the amount of 4) using laboratory samples of microbiological preparations were more effective in comparison with the active substance of chemical origin. The best scab control was provided by a laboratory sample of a three-component mixed preparation based on two fungal and one bacterial producer strain RK-1 + XK-1 + B-12 - 83.0-84.9%. The isolated samples are promising for research on the creation of microbiological preparations against apple scab.

Sign in / Sign up

Export Citation Format

Share Document