The relationship between the sources of lactobacillus isolates and their antimicrobial activity

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Jenan R. ALwaaili ◽  
Rahem E. ALzaidi
Keyword(s):  
Antimicrobial Activity ◽  
Dairy Products ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Biochemical Tests ◽  
Healthy Infants ◽  
Antibiotic Susceptibility Test ◽  
Pcr Products ◽  
The Relationship

Lactobacillus genus was isolated from two different sources, healthy infants feces and some dairy products, identify isolates by traditional methods and confirm diagnosis by molecular detection. Susceptibility was tested to some antibiotic then supernatants antimicrobial activity were tested against some pathogenic bacteria involved Escherichia coli O157:H7, Pseudomonas aeruginosa, Enterococcus faecalis , Staphylococcus aureus, Klebsiella sp., Salmonella typhimiurum and Clostridium sp.. Morphological, Microscopically and Biochemical tests results showed that eleven isolates were lactobacillus, DNA was extracted from isolates and PCR reaction was performed to confirm diagnosis using a specific primer for 16SrDNA in Lactobacillus genus, Lacto F and Lacto R, all isolates gave PCR products with molecular weight 231bp. Antibiotic susceptibility test showed that Lactobacillus isolates of healthy infants feces were appeared multi resistant to more than one of antibiotics and sensitive to chloramphenicol while Lactobacillus isolates of dairy products were less resistance to antibiotics and most of them are sensitive to antibiotics , also results showed varying inhibitory effect of all Lactobacillus isolates supernatants in growth of pathogenic bacteria in this study, healthy infants feces isolates showed highest inhibition zone than dairy products Lactobacillus isolates.

scholarly journals Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis

2017 ◽  
Vol 18 (4) ◽  
pp. 312-316 ◽  
Author(s):  
Aarti A Bohora ◽  
Sharad R Kokate
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Indicator Strain ◽  
Oral Bacteria ◽  
Diffusion Method

ABSTRACT Introduction The main goal of endodontics is the prevention of apical periodontitis. This is due to the presence of persistent pathogenic microorganisms, such as Enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. Lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. This study explores shifting the established paradigm of endodontic treatment, which has focused on eliminating all bacteria from the canal system and on elimination of the problematic bacteria through introducing probiotics. A preliminary work was performed to evaluate the possible effectiveness of probiotics in preventing the growth of E. faecalis. Materials and methods Two probiotic cultures Lactobacillus plantarum ATCC 8041 and Lactobacillus rhamnosus ATCC 7408 were selected to check their antimicrobial activity against E. faecalis ATCC 29212 by two methods: agar cup/well diffusion method and deferred antagonism test. Agar cup method A total of 0.5 mL of requisite test pathogen culture was inoculated into 20 mL of molten sterile Mueller and Hinton agar and cooled to 45 ± 2°C. Circular wells of diameter 10 mm were punched in each of the poured plates. Appropriately diluted test samples were added to the above-punched wells. The plates were incubated upright position at 37°C for 24 hours in aerobic conditions. Postincubation, zone of inhibition was measured. The cell-free supernatant of Lactobacillus species was also evaluated for antimicrobial activity. Deferred antagonism test The test probiotic strain was standardized to 0.1 optical density (OD) at 600 nm and inoculated in a 1 cm wide diametric streak across the surface of trypticase soy agar + yeast extract + calcium carbonate (TSYCa) agar using a sterile cotton swab. Then, the plate agar containing the test strain was incubated at 37°C for 24 hours under aerophilic condition and then standardized to 0.1 OD at 600 nm for overnight (18 hours, 37°C). A purified culture of indicator strain (pathogen) was streaked at right angles to the line of original producer growth. Postincubation plates were observed for the inhibition zone width of the indicator strain. Results Under the conditions of this study, Lactobacilli had an inhibitory effect on the growth of E. faecalis by agar cup method but not by deferred antagonism test. Conclusion This pilot study demonstrated that probiotics show a potential in root canal therapy. Clinical Significance If probioitcs are effective against endodontic pathogens, they can be potentially used as intracanal medicaments. This will be a novel concept of introducing bacteriotherapy in endodontics and replacing pathogenic bacteria by healthy bacteria, normal flora. How to cite this article Bohora AA, Kokate SR. Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis. J Contemp Dent Pract 2017;18(4):312-316.

Edible film based on Vicia faba shell gum, nano silver and Helichrysum arenarium essence

2019 ◽  
Vol 50 (2) ◽  
pp. 280-287 ◽  
Author(s):  
Farzane Jahantigh
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Natural Antioxidants ◽  
Inhibition Zone ◽  
Edible Film ◽  
Composition Analysis ◽  
Disk Diffusion Test ◽  
Content Type ◽  
The Difference

Purpose The inclusion of natural antioxidants and nanoparticles into edible coatings can improve their structure, functionality and applicability in foods. This study aims to determine the antibacterial effect of new nano-composite based on bean pod shell gum (BPSG), silver nanoparticle (SNP) and herbal essential oil (EO). Design/methodology/approach For this purpose, BPSG (4 per cent w/v), SNP (0.5-1 per cent w/v) and Helichrysum arenarium EO (1-2 per cent v/v) were mixed. After preparation the edible film, the antibacterial activity was tested on five food-borne pathogenic bacteria in two categories including two-gram positives and three-gram negatives bacteria. The EO composition was determined by GC–mass spectrometry and the antibacterial activity was tested using disk diffusion test. Findings Results showed that gram-positive bacteria were more susceptible than gram-negative bacteria. Increasing Helichrysum arenarium EO and SNP content increased antimicrobial activity of the edible film based on BPSG, so that the treatment containing 2 per cent v/v Helichrysum arenarium EO and 1 per cent w/v SNP led to the highest inhibition zone (8.1-13.1 mm) compared to treatment containing 1 per cent v/v Helichrysum arenarium EO and 0.5 per cent w/v SNP with inhibition zone range of 5.4-9.9 mm and the difference was statistically significant (p = 0.41). Also, the chemical composition analysis of the EO identified a total of 38 compounds in which a-pinene (32 per cent), 1,8-cineole (16 per cent), α-humulene (15 per cent) and ß-caryophyllene (8 per cent) were the main fractions. Other separated components accounted less than 29 per cent of the oil. Originality/value In general, SNP and Helichrysum arenarium EO improved the functional properties including the antimicrobial activity of the edible film based on BPSG, which increases the potential to be used as active packaging for fresh products.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO

2015 ◽  
Vol 1 (2) ◽  
pp. 126
Author(s):  
Urnemi ◽  
Sumaryati Syukur ◽  
Endang Purwati ◽  
Sanusi Ibrahim ◽  
Jamsari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Sds Page ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      

scholarly journals Estimation of antimicrobial activity of green mango ( mangifera indica L. ) Extract on the Growth of Bacteria

2018 ◽  
Vol 29 (1) ◽  
pp. 75
Author(s):  
Hiba Thamir Hussain
Keyword(s):  
Antimicrobial Activity ◽  
Mangifera Indica ◽  
Chemical Properties ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Similar Chemical ◽  
Infusion Method

 Abstract:-        Alternative herbal medicine has been used to treat various infections in human as well as in animal, natural plants contain phytoconstituent having similar chemical properties as of synthetic antibiotics ,due to that, the aim of this study was to investigte the biological properties of green mango (mangifera indica L.). The extraction of active phytoconstituent  of green mango was done by infusion method . Antimicrobial activity was carried out by agar well diffusion  method against two species of gram positive bacteria and five gram negative bacteria, at different concentrations 5%,10%,50% . The extract of concentration 5% has showed >2 mm zone of inhibition and after further screene can be considered as minimum inhibitory concentration (MIC) .    The mean zone of inhibition produced inhibition area range between 5mm  and 26 mm .The inhibition zone of  26 mm for  streptococcus has  showed  the   highest  zone of inhibition area. The infusion extract of green mango (mangifera indica L.) showed good inhibitory effect against almost the tested strains .      Green mango extract can serve as a potential  antimicrobial agent  for use in the  food industry and pharmaceutical manufacturing.

scholarly journals The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis

2021 ◽  
Author(s):  
Meral Yılmaz ◽  
Ömür Baysal ◽  
Ragıp Soner Silme
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Seed Quality ◽  
Seed Transmission ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Clavibacter Michiganensis ◽  
Origanum Vulgare ◽  
Ligand Interaction ◽  
Clavibacter Michiganensis Subsp

The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential oil (EO) against Clavibacter michiganensis subsp. michiganensis (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande) coated with different doses of EO derived from O. vulgare were inoculated with Cmm (1.8 × 10<sup>8</sup> CFU/mL). O. vulgare's EO showed a remarkable inhibition effect on the Cmm growth. The EO effect against Cmm was determined based on the parameters, such as the inhibition zone and bacterial population in a seed. The GC-MS analysis of EO showed that carvacrol is the major component (at 74.05%), which may inhibit the bacterial growth. Later, we have expanded our studies to determine the inhibitory effect of the EO's mode of action on the pathogenic bacteria with a molecular docking analysis based on the molecular protein-ligand interaction. The results showed that carvacrol has a strong interaction with the bacterial expansin protein (PDB 4JJO) of Cmm and the qPCR analyses confirmed the effect of the O. vulgare treatment against Cmm. This original approach has the prominent potential to prevent seed transmission of Cmm for seed quality in the world, suggesting a method for paving the way for Cmm disease management.

scholarly journals STUDY THE ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF PURIFIED BIOSURFACTANT PRODUCED FROM LACTOBACILLUS PLANTARUM AGAINST PATHOGENIC BACTERIA

2021 ◽  
Vol 52 (5) ◽  
pp. 1194-1206
Author(s):  
A . A. Jameel ◽  
N. H. Haider
Keyword(s):  
Surface Tension ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Lower Surface ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Clinical Samples ◽  
Adhesion Activity ◽  
Natural Media

This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial  and antiadhesion activities were evaluated against some pathogenic  bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant  at concentration 200 mg/ml on  S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified  biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM  and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805)  produced in MSM.

Inhibition of Coliform Bacteria by Lactic Acid Bacteria Isolated from Nham Hed (Fermented Mushroom)

2019 ◽  
Vol 886 ◽  
pp. 56-60
Author(s):  
Suttida Wittanalai ◽  
Keerati Tanruean ◽  
Phanida Mapoong
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Carbon Sources ◽  
Inhibition Zone ◽  
Viable Count ◽  
Coliform Bacteria ◽  
Control Food ◽  
Microscopic Morphology

Four samples of Nham Hed which fermented from different carbon sources; Homnil rice (HN), sticky rice (SR), LeumPua rice (LP) and rice berry (RB) were evaluated for total viable count of lactic acid bacteria (LAB). The rice berry Nham Hed samples showed the highest of total lactic bacteria count with values 2.83 x 105 cfu/g. The thirty one distinct colonies of LAB were isolated and categorized into 6 groups on the basis of their colony characteristics on MRS agar, microscopic morphology and biochemical characteristic. These 6 isolates were screened for their antimicrobial activity against 2 strains of coliform bacteria and it was found that LAB isolate E exhibited the highest antimicrobial activity against Escherichia coli and Enterobacter sp. which the zone of inhibition zone 14.22 ± 1.50 mm and 13.38 ± 1.25 mm, respectively. According to the morphology characterization, isolate E was probably classified to Weissella spp. These obtained results revealed the possibility for further characterization and purification the antimicrobial compound produced by the studied isolate as food bio preservatives to control food spoilage and pathogenic bacteria.

Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria

2017 ◽  
Vol 65 ◽  
pp. 10-15 ◽  
Author(s):  
Hamid Tebyanian ◽  
Afsaneh Bakhtiari ◽  
Ali Karami ◽  
Ashraf Kariminik
Keyword(s):  
Antimicrobial Activity ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Intestinal Flora ◽  
Inhibition Zone ◽  
Enteric Pathogens ◽  
Shigella Dysenteriae ◽  
Lactobacillus Bulgaricus ◽  
Lactobacillus Species ◽  
De Man

Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.

scholarly journals Production, Characterization, and Antimicrobial Activity of Mycocin Produced byDebaryomyces hanseniiDSMZ70238

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Safaa A. S. Al-Qaysi ◽  
Halah Al-Haideri ◽  
Zaid Akram Thabit ◽  
Wijdan Hameed Abd Al-Razzaq Al-Kubaisy ◽  
Jamal Abd Al-Rahman Ibrahim
Keyword(s):  
Antimicrobial Activity ◽  
Trichophyton Rubrum ◽  
Killer Toxin ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Toxin Production ◽  
Inhibitory Effect ◽  
Curvularia Lunata ◽  
Nacl Concentration ◽  
Inhibition Zones

The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced byD. hanseniiDSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm forStaphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans,andCandida neoformans, respectively. The largest inhibition zones were observed at pH 4.5 with inhibition zone of 16, 18, 17, 18, 11, and 12 mm for the same microorganisms. The results also showed that 25°C is the optimal temperature for toxin killing activity against all targeted microorganisms. In addition, the activity of killer toxin significantly inhibited the growth of fungal mycelia for all target pathogenic fungi and the percentages of inhibition were 47.77, 48.88, 52.22, and 61.11% forTrichophyton rubrum, Alternaria alternata, Trichophyton concentricum,andCurvularia lunata, respectively. The results showed the highest growth rate ofD. hanseniiDSMZ70238 under condition of 8% NaCl concentration, pH 4.5, and 25°C for 72 h.

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