Comparative Study of the Biologically Active Substances Composition and Content in Meadowsweet (Filipendula ulmaria (L.) Maxim) Crude Herbal Drugs (Herb, Leafs, Flowers) of Russian Origin

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Dmitry Olegovich Bokov ◽  
Tatyana Yuryevna Kovaleva ◽  
Valentina Alekseevna Ermakova ◽  
Daria Aleksandrovna Trashchenkova ◽  
Ekaterina Anatolievna Dorovskih ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Neurotropic Activity ◽  
Comparative Investigation ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Filipendula Ulmaria ◽  
Pharmacopoeial Monograph ◽  
Total Extractives ◽  
Layer Chromatography

Filipendula ulmaria (L.) Maxim. (Meadowsweet) is known in traditional medicine as anti-inflammatory, wound-healing,astringent and antibacterial remedy. However recent studies show that it also has neurotropic activity. In Russia meadowsweet flowers are used as crude herbal drugs (temporary pharmacopoeial monograph 42-1777-87), also leafs and herb are used in the traditional medicine. Objective of the study was to carry out comparative investigation of composition and content of major biologically active compounds (BAC) in Filipendula ulmaria herb, flowers and leafs by thin-layer chromatography, differential spectrophotometry with aluminum chloride reagent (total flavonoids in terms of rutoside), gravimetry (total extractives, extracted by water), permanganatometric titration (total tannins in terms of tannin). Rutoside, tannin, gallic acid and salicylic acid were identified in Filipendula ulmaria herb, flowers and leafs by TLC. Also we analyzed content of substances extracted by water, flavonoids and tannins. Total extractives, extracted by water in F. ulmaria herb is 13.12±0.10%, in leafs – 13.98±0.37%, in flowers – 18.09±0.17%. Total tannins in F. ulmaria herb is 11.87±0.47%, in leafs – 12.06±0.18%, in flowers – 12.26±0.29%. Total flavonoids in F. ulmaria herb 4.34±0.17%, in leafs – 6.98±0.23%, in flowers – 11.75±0.57%. The obtained data will be used for development of a pharmacopoeial monograph project "Filipendula ulmaria (L.) Maxim., herba" for inclusion in the State Pharmacopoeia of the Russian Federation.

scholarly journals INFLUENCE OF CONSERVATION METHOD (FREEZING AND DRYING) ON COMPOSITION AND CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN ROSACEAE FRUITS (CRUDE HERBAL DRUGS)

2019 ◽  
Vol 12 (1) ◽  
pp. 542
Author(s):  
Ekaterina Vyacheslavovna Sergunova ◽  
Dmitry Olegovich Bokov
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Oven Drying ◽  
Total Anthocyanins ◽  
Active Substances ◽  
Conservation Method

Objective: Now, five crude herbal drugs (CHD) of fruit morphological group (fruits) related to Rosaceae family included in the Russian State Pharmacopoeia XI and XIII editions: Fructus Rosae (Rosa cinnamomea L. and other species), Fructus Crataegi (Crataegus sanguinea Pall. and other species), Fructus Sorbi (Sorbus aucuparia L.), Fructus Rubi (Rubus idaeus L.), and Fructus Aroniae (Aronia melanocarpa [Michx.] Elliott).The aim of this study was to investigate the effect of freezing and oven drying on the composition and content of individual groups of biologically active substances (BAS) in Rosaceae fruits.Methods: The groups of BAS were investigated by UV spectrophotometry (flavonoids and anthocyanins), galvanostatic coulometry titration (organic acids), gravimetry (polysaccharides), and titration with potassium permanganate (tannins).Results: The relation between content of BAS (total free organic acids, total flavonoids, total anthocyanins, total tannins, and total polysaccharides) and fruits conservation method was determined. In frozen fruits total polysaccharides, total free organic acids, ascorbic acid content decreases by 5–7%, total flavonoids and total tannins content - 10–15%, total anthocyanins content - 25–30%. Fruits oven drying at 60–80°C leads to more significant losses of total anthocyanins, ascorbic acid, and total free organic acids.Conclusion: The study of BAS stability in frozen and dried fruits was lasting for 12 months. The duration of shelf life for frozen fruits is recommended. Freezing as a conservation method allows to preserve more BAS in Rosaceae fruits. Frozen CHD can be proposed as an alternative to fresh and dried СРВ for the preparation of allopathic and homeopathic medicines.

scholarly journals INFLUENCE OF CONSERVATION METHOD (FREEZING AND DRYING) ON COMPOSITION AND CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN ROSACEAE FRUITS (CRUDE HERBAL DRUGS)

2019 ◽  
Vol 12 (1) ◽  
pp. 542
Author(s):  
Ekaterina Vyacheslavovna Sergunova ◽  
Dmitry Olegovich Bokov
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Oven Drying ◽  
Total Anthocyanins ◽  
Active Substances ◽  
Conservation Method

Objective: Now, five crude herbal drugs (CHD) of fruit morphological group (fruits) related to Rosaceae family included in the Russian State Pharmacopoeia XI and XIII editions: Fructus Rosae (Rosa cinnamomea L. and other species), Fructus Crataegi (Crataegus sanguinea Pall. and other species), Fructus Sorbi (Sorbus aucuparia L.), Fructus Rubi (Rubus idaeus L.), and Fructus Aroniae (Aronia melanocarpa [Michx.] Elliott).The aim of this study was to investigate the effect of freezing and oven drying on the composition and content of individual groups of biologically active substances (BAS) in Rosaceae fruits.Methods: The groups of BAS were investigated by UV spectrophotometry (flavonoids and anthocyanins), galvanostatic coulometry titration (organic acids), gravimetry (polysaccharides), and titration with potassium permanganate (tannins).Results: The relation between content of BAS (total free organic acids, total flavonoids, total anthocyanins, total tannins, and total polysaccharides) and fruits conservation method was determined. In frozen fruits total polysaccharides, total free organic acids, ascorbic acid content decreases by 5–7%, total flavonoids and total tannins content - 10–15%, total anthocyanins content - 25–30%. Fruits oven drying at 60–80°C leads to more significant losses of total anthocyanins, ascorbic acid, and total free organic acids.Conclusion: The study of BAS stability in frozen and dried fruits was lasting for 12 months. The duration of shelf life for frozen fruits is recommended. Freezing as a conservation method allows to preserve more BAS in Rosaceae fruits. Frozen CHD can be proposed as an alternative to fresh and dried СРВ for the preparation of allopathic and homeopathic medicines.

An Overview on Phytochemical and Pharmacological Profile of Morus alba Linn.

2020 ◽  
Vol 16 ◽  
Author(s):  
Arpita Paul ◽  
Monami Rajiung ◽  
Kamaruz Zaman ◽  
Sushil Kumar Chaudhary ◽  
Hans Raj Bhat ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Morus Alba ◽  
Biologically Active ◽  
Pharmacological Profile ◽  
Pharmacological Activities ◽  
Significant Information ◽  
The Past ◽  
Comprehensive Information ◽  
The Family ◽  
White Mulberry

Background: Morus alba Linn. commonly known as white mulberry, belongs to the family Moraceae, is a promising traditional medicine. In Asia, besides its use in the preparation of delicacies, every part of this plant is utilized in traditional medicine. Over the past decade, studies related to identification and isolation of biologically active compounds, with flavonoids as the major class of phytoconstituents, from this plant has been reported. These phytoconstituents are not only found to be beneficial for the maintenance of general health but also are associated with a range of potential pharmacological activities such as antioxidant, anti-inflammatory, anti-diabetic, anticancer, hepatoprotective, cardioprotective, neuroprotective to name a few. Objective: This review aims to provide upgraded and comprehensive information regarding the phytochemical, ethnomedicinal use and pharmacological profile of the plant Morus alba Linn. Method: The significant information has been collected through various database viz. PubMed, Scopus, Web of Science, Science Direct based on the recent findings, using different terms of Morus alba. Results: The outcome of the study suggests that Morus alba is a multifunctional plant numerous phytochemicals, and possess a range of pharmacological activities. Conclusion: The data assembled on Morus alba will be beneficial to trigger research in various fields of pharmaceutical and allied science to explore the medicinal importance of this unique plant.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

Back to the Roots: Prediction of Biologically Active Natural Products from Ayurveda Traditional Medicine

2011 ◽  
Vol 30 (2-3) ◽  
pp. 181-187 ◽  
Author(s):  
Honey Polur ◽  
Tejal Joshi ◽  
Christopher T. Workman ◽  
Gandhidas Lavekar ◽  
Irene Kouskoumvekaki
Keyword(s):  
Natural Products ◽  
Traditional Medicine ◽  
Biologically Active ◽  
Active Natural

TYRAMINE, HISTAMINE, AND TRYPTAMINE CONTENT OF CHEESE

1974 ◽  
Vol 37 (7) ◽  
pp. 377-381 ◽  
Author(s):  
M. N. Voigt ◽  
R. R. Eitenmiller ◽  
P. E. Koehler ◽  
M. K. Hamdy
Keyword(s):  
Cheddar Cheese ◽  
The United States ◽  
Quantitative Information ◽  
Biologically Active ◽  
Blue Cheese ◽  
Physiological Importance ◽  
Fluorescence Measurements ◽  
Soft Cheese ◽  
Layer Chromatography ◽  
Derivatives Of

Because of the increasing knowledge of the physiological importance of biologically active amines in man and the importance of the presence of these amines in cheese, this study was done to obtain quantitative information for tyramine, tryptamine, and histamine in cheese available in the United States. The tyramine, histamine, and tryptamine contents of 156 samples of cheese purchased at retail stores were quantitated by thin-layer chromatography and fluorescence measurements of NBD-chloride derivatives of the amines. Tyramine was found in 81 of 85 Cheddar cheese samples examined. Extra-sharp, sharp, and medium Cheddar cheese samples contained average tyramine values of 0.27, 0.21, and 0.24 mg/g, respectively. Average tyramine contents were lower in mild and processed Cheddar (0.09 and 0.11 mg/g, respectively). The highest Cheddar cheese tyramine content was 0.7 mg/g. Tyramine was consistently found in all cheeses except in unripened soft cheese (Cottage). Histamine concentrations varied from nondetectable amounts to 2.6 mg/g in a Sap-Sago cheese sample. Twenty-four Cheddar cheese samples contained histamine with the highest amount being 1.3 mg/g. A domestic Blue cheese contained 2.3 mg/g. Tryptamine was uniformly low or completely absent in the Cheddar cheese samples. The highest tryptamine concentration (1.1 mg/g) was detected in a Blue cheese.

scholarly journals Heterogeneity of Mycolactones Produced by Clinical Isolates of Mycobacterium ulcerans: Implications for Virulence

2003 ◽  
Vol 71 (2) ◽  
pp. 774-783 ◽  
Author(s):  
Armand Mve-Obiang ◽  
Richard E. Lee ◽  
Françoise Portaels ◽  
P. L. C. Small
Keyword(s):  
Clinical Evidence ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Biologically Active ◽  
In Vivo Studies ◽  
Tissue Destruction ◽  
The Core ◽  
A Minor ◽  
Layer Chromatography

ABSTRACT Mycobacterium ulcerans is the causative agent of Buruli ulcer, a severe necrotizing skin disease endemic in tropical countries. Clinical evidence suggests that M. ulcerans isolates from Asia, Mexico, and Australia may be less virulent than isolates from Africa. In vivo studies suggest that mycolactone, a polyketide-derived macrolide toxin, plays a major role in the tissue destruction and immune suppression which occur in cases of Buruli ulcer. Mycolactones were extracted from 34 isolates of M. ulcerans representing strains from Africa, Malaysia, Asia, Australia, and Mexico. Thin-layer chromatography, mass spectroscopic analysis, and cytopathic assays of partially purified mycolactones from these isolates revealed that M. ulcerans produces a heterogeneous mixture of mycolactone variants. Mycolactone A/B, the most biologically active mycolactone species, was identified by mass spectroscopy as [M+Na]+ at m/z 765.5 in all cytotoxic isolates except for those from Mexico. Mycolactone C [M+Na]+ at m/z 726.3 was the dominant mycolactone species in eight Australian isolates, and mycolactone D [M+Na]+ m/z 781.2 was characteristic of two Asian strains. Mycolactone species are conserved within specific geographic areas, suggesting that there may be a correlation between mycolactone profile and virulence. In addition, the core lactone, [M+Na]+ m/z 447.4, was identified as a minor species, supporting the hypothesis that mycolactones are synthesized by two polyketide synthases. A cytopathic assay of the core lactone showed that this molecule is sufficient for cytotoxicity, although it is much less potent than the complete mycolactone.

The genus Sida L. – A traditional medicine: Its ethnopharmacological, phytochemical and pharmacological data for commercial exploitation in herbal drugs industry

2015 ◽  
Vol 176 ◽  
pp. 135-176 ◽  
Author(s):  
Biswanath Dinda ◽  
Niranjan Das ◽  
Subhajit Dinda ◽  
Manikarna Dinda ◽  
Indrajit SilSarma
Keyword(s):  
Traditional Medicine ◽  
Herbal Drugs ◽  
Commercial Exploitation ◽  
Pharmacological Data

scholarly journals Quality Control Assessment of an Ayurvedic Medicine - Durvadi Ghrita

2020 ◽  
Vol 11 (4) ◽  
pp. 754-758
Author(s):  
Sangita Kamaliya ◽  
Vaghela D B ◽  
Harisha C R ◽  
Shukla V J
Keyword(s):  
High Performance ◽  
Pollen Grains ◽  
Acid Value ◽  
Morphological Characters ◽  
Ayurvedic Medicine ◽  
Herbal Drugs ◽  
Herbal Drug ◽  
Saponification Value ◽  
Physicochemical Evaluation ◽  
Layer Chromatography

Background: Durvadi Ghrita is a Sneha Kalpana which is claimed to be effective in Madhumehajanya Timira (Diabetic Retinopathy).  In present study, it has been used for Nasya. Objective: Present study was planned to look out on herbal drugs used in the preparation of Durvadi Ghrita and standardization of drug by pharmacognostical and physicochemical parameters and HPTLC evaluation. Methods: Identification and authentication of all the raw drug was done by pharmacognostical study i.e. morphological characters, organoleptic characters and powder microscopy. Physicochemical evaluation and HPTLC of final product were done. Results: Pharmacognostical study of all the raw drugs of Durvadi Ghrita showed presence of oil globule, prismatic crystals of Durva. Lignified branched trichome, pollen grains of Utpala Kinjalaka. Trichome, border pitted vessels of Manjishtha. Collenchyma cells, border pitted vessel of Elvaluka. Lignified fibres, oil globules of Sita. Pitted fibres, pitted vessels of Usheera. Scalariform vessels, prismatic crystals of Musta. Pitted vessels and lignified fibres, crystal fibres of Chandana. Lignified cork, and stone cells of Padmaka etc. Pharmaceutical evaluation of Durvadi Ghrita showed results Specific Gravity 0.9125, Refractive Index 1.47, Acid Value 0.4608, Iodine Value 11.45 and Saponification Value 128.856. High Performance Thin Layer Chromatography, 12 spots were found at 254 nm and five spots were found at 366 nm. Conclusion: Identification and authentication of herbal drug used in the preparation of Durvadi Ghrita has been done. Pharmacognostical and physicochemical evaluation of prepared drug has been carried out which can be further useful for standardization of Durvadi Ghrita and other clinical researches.

scholarly journals Pharmacognostic, phytochemical and pharmacological aspects of Rosa x damascena Herrm. Georgian cultivar

2021 ◽  
Vol 16 (1) ◽  
pp. 001-010
Author(s):  
Nino Aleksidze ◽  
Ketevan Mchedlidze ◽  
Malkhaz Getia ◽  
Karen Mulkijanyan ◽  
Michel Frédérich ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Chemical Composition ◽  
Oil Industry ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Hot Plate ◽  
Rose Oil ◽  
Biological Potency ◽  
Rosaceae Family ◽  
The Rose

Rosa x damascena Herrm. represents one of the most popular and important plants of the Rosaceae family. For ages, rose oil, rose water and extracts from its flowers were used in perfumery, cosmetics, aromatherapy, and medicine due to diverse pharmacological efficacy. Nowadays, special attention is paid to the valorization of wastes from the rose oil industry in order to fully recover potent biologically active compounds. The present study describes in detail the microstructural features of the flower petals and provides data on chemical composition and biological potency of the aqueous extract of rose oil waste from the R. damascena Georgian cultivar. Up to 25 constituents were detected by HPLC-MS in the extract, and the content of total flavonoids in the extract reaches 2.29%. Amongst them hyperoside is dominating, which content is not less than 1%. Pharmacological evaluation of the extract on "Hot plate" and carrageenan-induced oedema models in mice revealed analgesic (42%) and anti-inflammatory (67%) effects of the extract. As well, the extract revealed antiplasmodial activity against Plasmodium falciparum (3D7) strain (IC50 =20.31 µg/mL)

Sign in / Sign up

Export Citation Format

Share Document