scholarly journals Nephroprotective Effect of Jatropha curcas Fruit Extracts Against Carbon Tetrachloride Induced Nephrotoxicity in Rats

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Komail M. ◽  
Narendra Babu A.
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Urine Analysis ◽  
Hematological Parameters ◽  
Blood Biochemical Parameters ◽  
Fruit Extracts ◽  
Blood Biochemical ◽  
Histopathological Studies ◽  
Nephroprotective Effect

The present study was undertaken to explore the nephroprotective potential of Jatrophacurcas fruit extracts against carbon tetrachloride (CCl4) induced nephrotoxicity in rats. Nephrotoxicity was induced by CCl4 (3ml/kg body weight) in animals. Blood biochemical parameters, urine analysis and histopathological studies were carried out to assess the nephroprotective effect. CCl4 administration induced significant nephrotoxicity in rats, which was evident from enhanced levels of albumin, potassium.From the obtained results it may be concluded that pretreatment of silymarin (50mg/kg dose orally) significantly reversed carbon tetrachloride induced nephrotoxicitywhere asJatrophacurcas methanol extract (250mg/kg body weight) showed significant effect against CCl4 induced nephrotoxicity in rats thanJatrophacurcas aqueous extract (p less than 0.001) for most of the blood biochemical parameters, hematological parameters as well in attenuation of pathological changes in kidney tissues.

scholarly journals Fortified S-Allyl L-Cysteine: Animal Safety, Effect on Retinal Ischemia, and Role of Wnt in the Underlying Therapeutic Mechanism

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Windsor Wen-Jin Chao ◽  
Yu-Kuang Chen ◽  
Howard Wen-Haur Chao ◽  
Wynn Hwai-Tzong Pan ◽  
Hsiao-Ming Chao
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Low Dose ◽  
Retinal Ischemia ◽  
Age Related Macular Degeneration ◽  
Retrograde Labelling ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Animal Safety

Purpose. Retinal ischemia is a medical condition associated with numerous retinal vascular disorders, such as age-related macular degeneration, glaucoma, and diabetic retinopathy. This in vitro cell and in vivo animal study investigated not only the protective effect of S-allyl L-cysteine (SAC, an active component of garlic) against retinal ischemia but also its associated protective mechanisms. Methods. Retinal ischemia was mimicked by raising the intraocular pressure to 120 mmHg for 1 hour in one eye. The effects of pre-/postischemic administration of vehicle vs. SAC 0.18 mg vs. SAC 0.018 mg vs. SAC 0.0018 mg treatments on retina cells were evaluated through cellular viability (MTT assay), flash electroretinograms (ERGs), and fluorogold retrograde labelling (retinal ganglion cell (RGC) counting). Also, protein immunoblot was utilized to assess the role of Wnt, hypoxia inducible factor (HIF)-1α, and vascular endothelium factor (VEGF) in the proposed anti-ischemic mechanism. Lastly, the safety of drug consumption was investigated for changes in the animal’s body weight, ERG waves, and blood biochemical parameters (e.g., glucose levels). Results. The characteristic ischemic changes including significant reduction in ERG b-wave ratio and RGC number were significantly counteracted by pre- and postischemic low dose of SAC. Additionally, ischemia-induced overexpression of Wnt/HIF-1α/VEGF protein was ameliorated significantly by preischemic low dose of SAC. In terms of the animal safety, no significant body weight and electrophysiological differences were observed among defined different concentrations of SAC without following ischemia. In low SAC dosage and vehicle groups, various blood biochemical parameters were normal; however, high and medium concentrations of SAC significantly lowered the levels of uric acid, Hb, and MCHC. Conclusion. This study shows that preischemic administration of low SAC dosage has been proved to be safe and most effective against rat retinal ischemia electrophysiologically and/or histopathologically. Moreover, counteracting the ischemia-induced overexpression of Wnt/HIF-1α/VEGF might presently explain SAC’s anti-ischemic mechanism.

Melatonin Improves Blood Biochemical Parameters and DNA Integrity in the Liver and Kidney of Hyperthyroid Male Rats

2020 ◽  
Keyword(s):  
Body Weight ◽  
Dna Fragmentation ◽  
Biochemical Parameters ◽  
Mature Male ◽  
Male Rats ◽  
Dna Integrity ◽  
Tissue Samples ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Liver And Kidney

The present study was carried out to investigate and compare the effect of hyperthyroidism either alone or in combination with melatonin on some blood biochemical parameters and DNA integrity in liver and kidney of male rats. For this purpose 75 mature male rats weighing 120-140g were randomly divided into five groups (control, hyperthyroid, hyperthyroid plus 1, 5 or 10 mg/kg bwt of melatonin, respectively). Hyperthyroidism was experimentally induced by daily I/P injection of L-thyroxine (0.2 mg/kg body weight). The melatonin treated groups were injected with the same dose of L-thyroxine followed by I/P injection of melatonin (1, 5 or 10 mg/kg, respectively). The study was carried out for 21 days. The last blood and tissue samples were collected one day after the end of the last injection (on the 22nd day). The results revealed that hyperthyroidism significantly increased the levels of urea and the activities of GOT, GPT, ALP as well as the percentage of DNA fragmentation in the liver and kidney. At the same time hyperthyroidism induced a significant decrease in level of creatinine. Treatment with the 3 doses of melatonin completely ameliorated the hyperthyroidism- induced increase in GPT and ALP, while 5 and 10 mg could completely counteract the hyperthyroidism- induced increase in urea and only the 10 mg melatonin could ameliorate the hyperthyroidism-induced increase in GOT activity. It is concluded that melatonin can ameliorate the hyperthyroidism-induced disturbance in blood biochemical parameters and DNA fragmentation.

scholarly journals Co-supplementation of colostrum powder on performance, intestinal morphology, blood biochemical parameters and antioxidant status of broilers in heat stress

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3419-3428
Author(s):  
Momammad Hussein Gorbannejad Parapary ◽  
◽  
Ali Nobakht ◽  
Yousef Mehmannavaz ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Carcass Traits ◽  
Intestinal Morphology ◽  
Breeding Period ◽  
Final Body Weight ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical

This experiment was conducted to investigate the effects of different levels of colostrum powder on performance, intestinal morphology, blood biochemical parameters and antioxidant status of broilers in heat stress. In this experiment 224 Ross-308 broiler chicks were used from 1 to 42 days in 5 treatments and 4 replicates (12 birds per replicate) in a completely randomized design. Treatments included: 1) control (without using colostrum), 2) 0.5% of colostrum, 3) 1% of colostrum, 4) 1.5% of colostrum, and 5) 2% of colostrum powder. Colostrum powder was added in first 10 days of broilers breeding period in their diets. Colostrum powder had significant effects on performance, carcass traits and intestinal cells morphology of broilers in heat stress (P < 0.05). In grower period the highest amounts of final body weight and body weight gain were obtained with 2% of colostrum powder. Colostrum powder in contrast to control improved the percentages of gizzard, liver, bursa of fabricious, thighs and breast (P > 0.05). The highest villies, the lowest crypts and the highest ratio of villies/crypts were obtained with colostrum powder adding (P > 0.05). Colostrum powder had no significant effects on blood biochemical parameters and antioxidant status of broilers. The overall resulted showed that in broilers in heat stress condition using colostrum powder up to 2% in starter period has beneficial effects on performance, carcass traits and intestinal morphology of broilers.

scholarly journals EFFECT OF GRINDED OLIVE LEAVES SUPPLIMENTATION IN MILK PRODUCTION AND ITS COMPONENTS AND SOME BLOOD TRAITS IN NATIVE DOES.

2016 ◽  
Vol 47 (5) ◽  
Author(s):  
AL-Musawi & et al.
Keyword(s):  
Body Weight ◽  
Milk Production ◽  
Milk Yield ◽  
Biochemical Parameters ◽  
Fat Percentage ◽  
Olive Leaves ◽  
Blood Biochemical Parameters ◽  
Beneficial Effects ◽  
Blood Biochemical ◽  
Lactating Goats

The present study was carried out at the Animal Farm College of Veterinary Medicine University of Baghdad during the period from 20/4/2015 to1/6/2015. This study was conducted to investigate the effect of olive leaves (grinded) supplementation to diet on milk yield, composition and some blood biochemical  parameters.Ten local lactating goats and its mean initial live body weight41.38± 0.46 kg, and 2-3.5 years old were allocated according to their body weight and milk yield  into two groups (5does/group). The first one (G1) was control, the second (G2) fed on diet with 2% olive leaves powder (of diet weight). The animals in both groups were fed the experimental diets 2 % DM of live body weight plus alfalfa hay (1 kg / head/day). Results showed the olive leaves powder supplementation had no significant effect on the average body weight in the end of experiment, while the milk production was  significantly (P<0.05)increased in (G2) being 13.68 kg/Doe compared with 10.76 kg/Doe(G1), milk compositions (lactose. protein and fat percentage) and milk energy value did not significantly different between (G1) and (G2).Moreover blood biochemical parameters did not different in both groups. It can be concluded that olive leaves powder exerted have beneficial effects on the performance of lactating goats and no effect on blood biochemical parameters.

scholarly journals Nephroprotective Effect of the Herbal Composition BNO 2103 in Rats with Renal Failure

2020 ◽  
Vol 88 (4) ◽  
pp. 47
Author(s):  
Sergii K. Shebeko ◽  
Vladyslava V. Chernykh ◽  
Kateryna O. Zupanets
Keyword(s):  
Renal Failure ◽  
Nitrogen Metabolism ◽  
Biochemical Parameters ◽  
Combined Therapy ◽  
Complex Treatment ◽  
Blood Biochemical Parameters ◽  
Excretory Function ◽  
Blood Biochemical ◽  
Nephroprotective Effect ◽  
Renal Excretory Function

(1) Background: this research aims at studying the nephroprotective properties of BNO 2103 in a model of chromate-induced renal failure in rats and at proving the possibility of using BNO 2103 in clinical practice for the complex treatment of chronic kidney disease (CKD). (2) Methods: fifty rats divided into five groups were studied. The drugs BNO 2103, Prednisolone and Lespephril were administered within 20 days. The excretory function and the functional state of kidneys, blood biochemical parameters and indicators of nitrogen metabolism were determined. (3) Results: under the influence of BNO 2103, there was a significant improvement in renal excretory function, in nitrogen metabolism and blood biochemical parameters compared with the control pathology group. BNO 2103 also outperformed the comparators in most indicators. (4) Conclusions: BNO 2103 has demonstrated nephroprotective, hypoazotemic and diuretic effects; and can be used to implement to the combined therapy of CKD.

The effects of dietary deoxynivalenol (DON) on selected blood biochemical and hematological parameters in pre-pubertal gilts

2015 ◽  
Vol 18 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Ł. Zielonka ◽  
M. Gajęcka ◽  
M. Tarasiuk ◽  
M. Gajęcki
Keyword(s):  
Body Weight ◽  
Placebo Group ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Energy Resources ◽  
Low Doses ◽  
Blood Biochemical ◽  
Adaptive Mechanisms ◽  
Daily Dose ◽  
Biochemical Profiles

Abstract The aim of this study was to determine whether exposure to low doses of DON leads to changes in serum hematological and biochemical profiles. In the experiment, pre-pubertal gilts (with body weight of up to 25 kg) were administered DON per os at a daily dose of 12 μg/kg BW (group E, n=18) or a placebo (group C, n=21) over a period of 42 days. Blood for analysis was sampled seven times at weekly intervals. Minor, but statistically significant changes were observed in selected indicators, particularly in weeks 2 and 3 in values of biochemical parameters such AlAT, AspAT, Pin and Fe, and in values of hematological parameters such as RBC, MCV, MCHC, PLT, MPV, WBC, neutrophil, eosinophil, lymphocyte and monocyte counts. In group E, a decreasing trend of the values was observed in most cases, except for RBC, PLT, eosinophil, lymphocyte and monocyte counts. Similar results were obtained in the last two weeks of the experiment. Initially, exposure to DON has a stimulating effect, which is eliminated when adaptive mechanisms are triggered. The results of the study indicate that the intensity of biotransformation processes varies subjected to the body's energy resources.

scholarly journals Blood cells and some hematological parameters of red drum (Linnaeus, 1766) in Vietnam

2022 ◽  
Vol 82 ◽  
Author(s):  
D. H. Quyet
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Water Environment ◽  
River Mouth ◽  
Red Drum ◽  
Sciaenops Ocellatus ◽  
Erythrocyte Morphology ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Parameters Analysis

Abstract This research focuses on hematological characteristics, erythrocyte morphology and some biochemical parameters of red drum Sciaenops ocellatus (Perciformes: Sciaenidae), cultured in natural water environment in areas near river mouth (L1), estuaries (L2) in Ha Tinh province and coastal areas (L3) in Nha Trang city, Khanh Hoa province of Vietnam. A total of 18 speciments were examined in research, six in each location. Blood was drawn from the tail vein, using a microscope to research morphology and automated gauges to determine blood biochemical parameters. Analysis of blood samples showed that the rate of red drum’s erythrocyte morphology disorders in all three locations was quite high. The two main types of disorders were nuclear deformity and nuclear-matter distribution. Changes in erythrocyte size, shape and nuclear were related to salt concentration at culture locations. Blood hemoglobin content was stable in all three regions. Other hematological parameters such as the number of erythrocytes, blood biochemical parameters (glucose, SGOT, SGPT, urea, creatine, plasma iron, albumin, and protein) have differences among the locations, which showed the different reactions of the same species with different environmental conditions.

scholarly journals The effects of intermittent fasting on liver physiology and metabolism in mice

2020 ◽  
Author(s):  
Jianbo Ma ◽  
Yan Cheng ◽  
Qiang Su ◽  
Wen Ai ◽  
Ling Gong ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Metabolic Profiling ◽  
Biochemical Parameters ◽  
Liver Weight ◽  
Intermittent Fasting ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Ad Libitum ◽  
Underlying Mechanisms

Abstract Background: Intermittent fasting, as an emerging diet concept, has been widely practiced in the global population. A broad spectrum of health benefits has been reported in animal models and in humans for intermittent fasting. However, the underlying mechanisms remain largely elusive. In this study, we aimed to explore the effects and potential mode-of-action of intermittent fasting in mouse models, with focus on the liver.Methods: C57BL/6 mice were divided into five groups of 8-14 each, including 30-days ad libitum group, 30-days intermittent fasting group, 60-days ad libitum group, 60-days intermittent fasting group and refeeding group (30-days intermittent fasting followed by 30-days ad libitum). The food intake, body weight, liver weight and the blood biochemical parameters were detected. Targeted metabolic profiling of liver was performed.Results: We found that daily 12-hour intermittent fasting for one or two months significantly reduced the cumulative food intake, compared with the mice fed ad libitum. Fasting resulted in significantly reduced liver weight, with minimal effect on body weight. This effect on the liver by one month fasting could not be reversed by following one-month ad libitum feeding. Among the measured blood biochemical parameters, glucose level was decreased, while alkaline phosphatase was increased in the fasting mice. Surprisingly, targeted metabolic profiling revealed the global elevation of metabolites in the livers of fasting mice. These metabolic molecules include ATP, NADP, NADPH and succinate that are essentially involved in the citric acid cycle and oxidative phosphorylation. Conclusion: Daily 12-hour intermittent fasting for one to two months significantly reduced liver weight in mice, which is associated with enhanced liver metabolism.

DYNAMICS OF BODY WEIGHT AND HEMATOLOGICAL INDICATORS OF BLOOD OF ROMANOV LAMBS AT VARIOUS LEVELS OF FEEDING

2020 ◽  
pp. 28-31
Author(s):  
A.S. KHODOV ◽  
◽  
V.G. DVALISHVILI ◽  
Keyword(s):  
Body Weight ◽  
Energy Metabolism ◽  
Biochemical Parameters ◽  
Crude Protein ◽  
The Body ◽  
Metabolic Energy ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Hematological Indicators

The dynamics of the body weight of sheepand lambs of the Romanov breed at the age of 3-10 months with diff erent levels of feeding (energy and protein), as well as blood biochemical parameters characterizing nitrogenous and energy metabolism are shown. With an increase in the level of metabolic energy and crude protein in the diets, nitrogen and energy metabolism of young animals proceeded more intensively.

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