In vitro Antiradical activity and Hepatoprotective potential of Herbal extract from Medicinal plants of Rosaceae family

2021 ◽  
Vol 16 (11) ◽  
pp. 109-121
Author(s):  
Sonia Singh ◽  
Meenakshi Bajpai ◽  
Pradeep Mishra
Keyword(s):  
Carbon Tetrachloride ◽  
Olive Oil ◽  
Antiradical Activity ◽  
Herbal Extract ◽  
Appreciable Amount ◽  
Total Phenolic ◽  
Pyrus Pyrifolia ◽  
Standard Drug ◽  
Group I

The experimental study was designed to evaluate the in vitro antioxidant activity and hepatoprotective potential of ethanolic fruit extract of Pyrus pyrifolia (Burm.f.) Nakai. and Prunus avium Linn. in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Pharmacognostic evaluation of fruits and their extracts was carried out according to the WHO quality control of herbal drugs. The antiradical activity of ethanol extracts of P. pyrifolia (Burm.f.) Nakai and P. avium L. was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and nitric oxide free radical scavenging assay. The Wistar albino rats were randomly divided into seven equal groups (n = 5). Group I was treated as normal control. Group II received 0.2% carbon tetrachloride (CCL4) in olive oil (8 mL/kg, i.p) on 14th day; Groups III as standard control contained silymarin (100 mg/kg, p.o) as standard drug once daily for 14 days. Group IV and V were given P. pyrifolia (Burm.f.) Nakai extract (200 and 400 mg/kg, p.o. in olive oil) for fourteen days; Group VI and VII received P. avium Linn. extract (200 and 400 mg/kg p.o. in olive oil) for fourteen days. Group III to VII animals were treated with 0.2% CCl4 in olive oil (8mL/kg, i.p) on the 14th day after the administration (1 hour) of the standard drug and test samples. Histopathological analysis and measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), total albumin (TA) and total bilirubin (TB) were performed. Ethanol extract of P.avium L. demonstrated stronger antioxidant and higher total phenolic as well as total flavonoid content than P. pyrifolia (Burm.f.) Nakai. Both the extracts EEPP and EEPA (200 and 400 mg/kg) significantly normalized the CCl4-elevated levels of ALT, AST and ALP. P. pyrifolia (Burm.f.) Nakai and P. avium L. are the edible seasonal fruits that possessed an appreciable amount of phenolic and flavonoid components and could provide considerable protection against CCl4 hepatotoxicity in rodents that may be related to its antioxidant properties and also useful in preventing several degenerative and life threatening diseases.

scholarly journals In Vitro Assessment of Antioxidant, Thrombolytic, Antimicrobial Activities of Medicinal Plant Pandanus odoratissimus L. Leaves Extract

2020 ◽  
Vol 12 (3) ◽  
pp. 379-390
Author(s):  
F. I. Penu ◽  
S. M. Ivy ◽  
F. Ahmed ◽  
J. Uddin ◽  
M. S. Hossain ◽  
...  
Keyword(s):  
Soluble Fraction ◽  
Blood Clot ◽  
Folk Medicine ◽  
Maximum Level ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Pandanus Odoratissimus

The present study was carried out to investigate phytochemical, antioxidant; antimicrobial, thrombolytic activity and estimate total phenolic, total flavonoid content of Pandanus odoratissimus (p.odoratissimus) leaves of methanol extract. In thrombolytic activity, aqueous soluble fraction (AQSF) exhibited highest percentage (46.58 %) of potential to lyse blood clot compared to standard drug streptokinase (69.52 %). In antimicrobial assay, dichloromethane soluble fraction (DCMSF) explored the highest diameter of clear zone of inhibition against both gram positive (19.60 ± 0.12 mm) and gram negative (20.00 ± 0.20 mm) bacteria compared to standard antibiotic, Kanamycin (50.00 ± 0.19). Levels of antioxidant were determined by DPPH assay followed by calculated IC50 values of different Kupchan extracts. The methyl soluble fraction (MSF) showed the lowest level of IC50 value (36.70 ± 0.32 µg/mL) in comparison to ascorbic acid (12.48 ± 0.09 µg/mL) while MSF disclosed the maximum level (62.19 ±  0.26 mg of GAE/g of extract) of total phenolic content in the extracts of P. odoratissimus. This study was conducted to validate the P. odoratissimus leaves used as a folk medicine such as, antioxidant, thrombolytic, and antimicrobial potential.

scholarly journals Biological Screenings of Two Araceous Plants Growing in Bangladesh

2012 ◽  
Vol 10 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Tanjina Hoque ◽  
Md Al Amin Sikder ◽  
Mohammad A Kaisar ◽  
Abu Asad Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Soluble Fraction ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Vincristine Sulphate ◽  
Gallic Acid Equivalent

Two Bangladeshi medicinal plants from Araceae family, Alocasia indica and Steudnera virosa, have been investigated for their in vitro thrombolytic, membrane stabilizing antimicrobial activities and generalized    toxicity. The total phenolic content was also determined and expressed in gallic acid equivalent. The membrane stabilizing activity was assessed by heat and hypotonic solution induced methods. The carbon tetrachloride soluble  fraction (CTCSF) of A. indica and aqueous soluble fraction (AQSF) of S. virosa demonstrated strong membrane stabilizing activity. Different extractives of A. indica and S. virosa showed moderate thrombolytic activity. Among the two plants, the methanoic crude extract and its chloroform soluble partitionate of S. virosa revealed moderate inhibition of microbial growth. In brine shrimp lethality bioassay, the pet-ether and carbon tetrachloride soluble fractions of A. indica and the crude extract and its pet-ether soluble fraction of S. virosa were found to be as lethal as    Vincristine sulphate after 24 hours observation on shrimp nauplii.   DOI: http://dx.doi.org/10.3329/dujps.v10i2.11794   Dhaka Univ. J. Pharm. Sci. 10(2): 131-135, 2011(December)  

scholarly journals Enrichment of Phenolic Compounds from Olive Mill Wastewater and In Vitro Evaluation of Their Antimicrobial Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Saleh Abu-Lafi ◽  
Mahmoud Sami Al-Natsheh ◽  
Reem Yaghmoor ◽  
Fuad Al-Rimawi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Acid Value ◽  
Antimicrobial Activities ◽  
Olive Mill Wastewater ◽  
Total Phenolic ◽  
The Stability ◽  
Olive Mill

The production of olive oil generates massive quantities of by-product called olive mill wastewater (OMWW). The uncontrolled disposal of OMWW poses serious environmental problems. The OMWW effluent is rich in several polyphenolic compounds. Liquid-liquid extraction of OMWW using ethyl acetate solvent was used to enrich phenolic compounds under investigation. Total phenolic and flavonoid content and antioxidant activity of the extract were determined. HPLC coupled to photodiode array (PDA) detector was used to analyze the main three phenolic compounds of OMWW, namely, hydroxytyrosol, tyrosol, and oleuropein. The antimicrobial activity of the extract was also investigated. Additionally, the OMWW extract was used as natural preservative and antioxidants for olive oil. Results showed that OMWW is very rich in phenolic compounds and has strong antioxidant activity. HPLC analysis showed that the extract contains mainly hydroxytyrosol and tyrosol but no oleuropein. The OMWW extract showed also positive activities as antibacterial (gram positive and gram negative) and antifungal as well as activities against yeast. The addition of OMWW extract to olive oil samples has an effect on the stability of olive oil as reflected by its acid value, peroxide value, K232 and K270, and total phenolic content.

scholarly journals POTENTIAL ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL EVALUATION OF EXTRACTS OF LEUCAS ASPERA USING IN VITRO MODELS

2016 ◽  
Vol 8 (12) ◽  
pp. 292 ◽  
Author(s):  
Tahareen S. ◽  
Shwetha R. ◽  
Myrene R. D.
Keyword(s):  
Inflammatory Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Leucas Aspera ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Stabilization Assay

<p><strong>Objective: </strong>To evaluate the potential antioxidant, anti-inflammatory and antibacterial activities of aqueous and methanolic extracts of leaves of <em>Leucas aspera</em> (Thumbae).</p><p><strong>Methods: </strong>Phytochemical screening of the leaves of <em>L. aspera</em> was followed by analysis of antioxidant activity by means of DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity. <em>In vitro</em> anti‐inflammatory activity was evaluated using lipoxygenase inhibition, albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of anti‐inflammatory activity. Linear regression analysis was used to calculate half maximal inhibitory concentration, IC50 value. The zone of inhibition was performed against common pathogens to determine the antimicrobial activity at different concentrations of plant extracts (60%, 70%, 80%).</p><p><strong>Results: </strong>The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, tannins, flavonoids, glycosides, xanthoproteins, and phenols. The total phenolic and flavonoid content was found to be 2.25±0.04 mg GAE/g (gallic acid equivalents) and 1.2±0.05 mg QE/g (Quercetin equivalents) of fresh weight tissue respectively. The IC50 values for hydrogen peroxide scavenging activity were found to be 244.6 µg/ml. The extract inhibited the lipoxygenase enzyme activity with an IC50 value of 356.3 µg/ml. Maximum inhibition of heat-induced protein denaturation of 69% was observed at 400 μg/ml, IC50 249.6 μg/ml. Proteinase activity was also significantly inhibited (IC50 = 421.6 μg/ml). Membrane stabilization assay attributed minor protection by the leaf extract with an IC50 of 206.7. It was observed that <em>E. coli</em> were inhibited at all concentrations, followed by <em>Klebsiella</em> and <em>Pseudomonas</em>.</p><p><strong>Conclusion: </strong>Results indicate that L. aspera possess anti-inflammatory properties due to the strong occurrence of polyphenolic compounds such as alkaloids, flavonoids, tannins and steroids that serve as free radical inhibitors or scavenger. Compounds of the plant L. aspera may hence be used as lead compounds for designing potent anti-inflammatory drug which can be used for treatment of various diseases.</p><p> </p>

scholarly journals In vitro Thrombolytic and Membrane Stabilizing Studies of Brassica rapa subsp. chinensis (L.) Hanelt

2015 ◽  
Vol 17 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Nushrat Jahan ◽  
SM Ashikur Rahman ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brassica Rapa ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

Brassica rapa subsp. chinensis (L.) Hanelt is a popular herb in Bangladesh. In the present studies, the plant has been assessed to evaluate its in vitro thrombolytic and membrane stabilizing properties. Initially, the plant was collected, processed and extracted with methanol and the concentrated crude methanol extract was fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble materials. All extractives, including the parent extract were subjected to thrombolytic and membrane stabilizing bioassays. In case of thrombolytic study, the carbon tetrachloride soluble fraction exhibited highest clot lysis. Here, streptokinase and distilled water were used as positive and negative control, respectively. However, in the assay for membrane stabilizing activity, the crude methanol extract along with other extractives were capable to inhibit hemolysis of erythrocyte membrane in hypotonic solution- and heat- induced conditions, which indicated anti-inflammatory property of the samples. Acetyl salicylic acid was used as standard drug in this assay. This represents the first report of thrombolytic and membrane stabilizing activities of B. rapa subsp. chinensis. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22336 Bangladesh Pharmaceutical Journal 17(2): 172-176, 2014

scholarly journals Anti-Obesity Attributes; UHPLC-QTOF-MS/MS-Based Metabolite Profiling and Molecular Docking Insights of Taraxacum officinale

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4935
Author(s):  
Zain Ul Aabideen ◽  
Muhammad Waseem Mumtaz ◽  
Muhammad Tayyab Akhtar ◽  
Hamid Mukhtar ◽  
Syed Ali Raza ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Pancreatic Lipase ◽  
Positive Impact ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Taraxacum Officinale ◽  
Binding Energies ◽  
Total Phenolic ◽  
Standard Drug

The naturopathic treatment of obesity is a matter of keen interest to develop efficient natural pharmacological routes for disease management with low or negligible toxicity and side effects. For this purpose, optimized ultrasonicated hydroethanolic extracts of Taraxacum officinale were evaluated for antiobesity attributes. The 2,2-diphenyl-1-picrylhydrazyl method was adopted to evaluate antioxidant potential. Porcine pancreatic lipase inhibitory assay was conducted to assess the in vitro antiobesity property. Ultra-high performance chromatography equipped with a mass spectrometer was utilized to profile the secondary metabolites in the most potent extract. The 60% ethanolic extract exhibited highest extract yield (25.05 ± 0.07%), total phenolic contents (123.42 ± 0.007 mg GAE/g DE), total flavonoid contents (55.81 ± 0.004 RE/g DE), DPPH-radical-scavenging activity (IC50 = 81.05 ± 0.96 µg/mL) and pancreatic lipase inhibitory properties (IC50 = 146.49 ± 4.24 µg/mL). The targeted metabolite fingerprinting highlighted the presence of high-value secondary metabolites. Molecular-binding energies computed by docking tool revealed the possible contribution towards pancreatic lipase inhibitory properties of secondary metabolites including myricetin, isomangiferin, icariside B4, kaempferol and luteolin derivatives when compared to the standard drug orlistat. In vivo investigations revealed a positive impact on the lipid profile and obesity biomarkers of obese mice. The study presents Taraxacum officinale as a potent source of functional bioactive ingredients to impart new insights into the existing pool of knowledge of naturopathic approaches towards obesity management.

In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kenneth O. Okolo ◽  
Orish. E. Orisakwe
Keyword(s):  
Gas Chromatography ◽  
Carbon Tetrachloride ◽  
Ethanol Extract ◽  
Liver Function Tests ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
Group I ◽  
Hepatoprotective Effects

AbstractObjectivesThis study has characterized the phytoconstituents and evaluated the in vitro antioxidant and hepatoprotective effects of Pleurotus tuber-regium induced by carbon tetrachloride (CCl4). In vitro antioxidant assay of ethanol extract of P. tuber-regium and gas chromatography-mass spectrometry analyses to identify the phytoconstituents were carried out.MethodsSixty rats were divided into six groups of 10 animals in each group and treated as follows for 13 weeks. Group I (control) received 3 mL/kg olive oil intraperitoneal twice weekly in addition to feed and water ad libitum. Group II received CCl4 3 mL/kg twice weekly. Groups III, IV and V received 100, 200 and 500 mg/kg wild edible P. tuber-regium mixed with feed by ingestion daily in addition to 3 mL/kg CCl4 twice weekly, respectively. Group VI received 500 mg P. tuber-regium daily. Liver and body weights were recorded. Liver function tests, oxidative stress biomarkers, bilirubin, ascorbic acid and α-tocopherol were assayed. Histopathology of the liver was carried out. The gas chromatography-mass spectroscopy analysis yielded 10 antioxidants.ResultsCCl4 increased the levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin and malondialdehyde from 24±1.778 iu/L, 53±3.7 iu/L, 257±19 iu/L, 0.45±0.03 mg/dL and 1.90±0.42 μmol/L in the control group to 48±2.5 iu/L, 81±2.10 iu/L, 495±38 iu/L, 1.20±0.09 mg/dL and 14.0±2.6 μmol/L in the treated group, respectively.ConclusionsP. tuber-regium prevented the necrosis, edema and vein congestion observed in the CCl4-only group. P. tuber-regium is effective in protecting the liver against CCl4-induced damage.

scholarly journals Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

2021 ◽  
Vol 19 (1) ◽  
pp. 586-599
Author(s):  
Muhammad Shafiq Khan ◽  
Shahid Aziz ◽  
Muhammad Zakryya Khan ◽  
Zafar Mahmood Khalid ◽  
Muhammad Riaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate Fraction ◽  
Treated Group ◽  
Diabetic Control ◽  
Total Phenolic ◽  
Control Group ◽  
Standard Drug ◽  
Aqueous Fraction ◽  
Rubia Cordifolia ◽  
Group I

Abstract Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.

scholarly journals In-vitro Antioxidant and Anti-inflammatory Activities of Quisqualis indica Linn. Leaves Extract

2019 ◽  
pp. 1-13
Author(s):  
Pallavi Pal ◽  
Ajeet Singh
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Aim: In this study antioxidant and anti-inflammatory effect of ethanolic extract of Quisqualis indica leaves was evaluated. Study Design: In-vitro analysis of Quisqualis indica leaf extract. Place and Duration of Study: Molecular Biology laboratory, Department of Biotechnology, G.B Pant Engineering College, Pauri, between July 2015 and July 2016. Methods: Non-enzymatic and enzymatic assays such as DPPH (1, 1diphenyl-2-picryl hydrazyl), FRAP assay, superoxide dismutase SOD (EC 1.15.1.1), catalase (EC 1.11.1.6), for radical scavenging activity of ethanolic extracts of Quisqualis indica Linn. plant leaves had done. For estimation of anti-inflammatory action, two methods were employed: protein denaturation method and membrane stabilization method. Results: Ethanolic extract of leaves on higher concentration had better antioxidant potential when compared with reference standard ascorbic acid. They exhibited strong antioxidant radical scavenging activity values for ethanolic extract of leaves. Results of anti-inflammatory method suggested better potential values for ethanolic extract and compared with standard drug diclofenac sodium respectively. A significant relationship between antioxidant, anti-inflammatory capacity and total phenolic content was examined, indicating that phenolic compounds are the major contributors for the antioxidant and anti-inflammatory properties of this plant. Conclusion: Ethanolic extract of Q. indica exhibited strong anti-inflammatory and antioxidant activity and this can be used for designing novel drug inhibitors with better efficacy.

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