Significance of PAM Histochemical Reaction in Delineating Macrophages

Introduction. The aim of the study is to perform the anatomical and histological analysis of the roots of Rheum palmatum L. and Rheum officinale B., and also to check histochemical reactions for the presence of anthracene derivatives. Material and methods. The methodological basis of the anatomical and histological studies was the standard method for the preparation of micro-preparations of roots of Rheum palmatum L. and Rheum officinale B. described in the State Pharmacopoeia of the Russian Federation, XIV edition (OFS.1.5.3.003.15). Results and discussion. Anatomical and histological studies of Rheum palmatum L. and Rheum officinale B. raw materials confirmed the roots of Rheum palmatum L. to have classical secondary structure, while the roots of Rheum officinale B. have classical primary structure. The histochemical reaction to anthracene derivatives with 10% alkali solution was carried out, it resulted in cherry-red staining of core rays and some structures of cortical parenchyma of the fragment of Rheum palmatum L., while the roots of Rheum officinale B. failed to stain. Conclusions. The anatomical and histological studies of the rhizomes and roots of Rheum palmatum L. and Rheum officinale B. demonstrate the difference in the structure of closely related species of plants. It makes possible to identify the medicinal raw material of these plants.

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THE FINE STRUCTURAL LOCALIZATION OF ACETYLCHOLINESTERASE ACTIVITY IN THE RETINA AND OPTIC NERVE OF RABBITS

Journal of Histochemistry & Cytochemistry ◽

10.1177/19.2.85 ◽

1971 ◽

Vol 19 (2) ◽

pp. 85-96 ◽

Cited By ~ 41

Author(s):

E. REALE ◽

L. LUCIANO ◽

M. SPITZNAS

Keyword(s):

Endoplasmic Reticulum ◽

Optic Nerve ◽

Ganglion Cells ◽

Plexiform Layer ◽

Amacrine Cells ◽

Acetylcholinesterase Activity ◽

Horizontal Cells ◽

Histochemical Reaction ◽

Fiber Layer ◽

Structural Localization

In the rabbit retina acetylcholinesterase activity is localized in the perinuclear cisterna, in the cisternae of the rough surfaced endoplasmic reticulum and in the Golgi apparatus of ganglion cells and amacrine cells. The histochemical reaction is positive also in the rough surfaced endoplasmic reticulum of some horizontal cells. The highest activity is seen in the internal plexiform layer; because of artifacts caused by the diffusion of the enzyme, a clear demonstration of relation of the positivity to one or the other regular components of this layer, however, is not possible. Myelinated fibers which exhibit acetylcholinesterase activity and are most probably efferent are found in the internal plexiform layer. In the retinal nerve fiber layer and in the optic nerve only a few fibers show a positive reaction.

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Histology and Fine Structure of the Accessory Boring Organ of Polinices lewisi (Gastropoda, Prosobranchiata)

Journal of the Fisheries Research Board of Canada ◽

10.1139/f69-135 ◽

1969 ◽

Vol 26 (6) ◽

pp. 1451-1457 ◽

Cited By ~ 5

Author(s):

F. R. Bernard ◽

J. W. Bagshaw

Keyword(s):

Electron Microscopy ◽

Fine Structure ◽

Central Region ◽

Brush Border ◽

Histochemical Reaction

The histology of the accessory boring organ of the naticid clam drill Polinices lewisi (Gould) was investigated by means of electron microscopy and standard histological techniques.The organ consists of two distinct epithelial regions, one producing typical mucin histochemical reaction and a central region which secretes a proteinaceous substance. Overlying the central epithelium is a brush border consisting of numerous microvilli passing through a matrix of protein and chitin.

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Further data on a histochemical reaction as applied to the thin-layer chromatography of sterols

Journal of Chromatography A ◽

10.1016/s0021-9673(00)89030-5 ◽

1975 ◽

Vol 115 (1) ◽

pp. 183-189 ◽

Cited By ~ 1

Author(s):

Luiz Biella Souza Valle ◽

Ricardo Martins Oliveira-Filho ◽

Sidney Augusto Camara

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Histochemical Reaction ◽

Layer Chromatography

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3β-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN THE HUMAN FOETAL ADRENAL CORTEX

Acta Endocrinologica ◽

10.1530/acta.0.0480423 ◽

1965 ◽

Vol 48 (3) ◽

pp. 423-428 ◽

Cited By ~ 14

Author(s):

M. Niemi ◽

A. H. Baillie

Keyword(s):

Dehydrogenase Activity ◽

Adrenal Cortex ◽

Substrate Binding ◽

Hydroxysteroid Dehydrogenase ◽

List Type ◽

Histochemical Reaction ◽

Enzyme Substrate ◽

Human Male ◽

Foetal Life ◽

Free Steroid

ABSTRACT 3β-Hydroxysteroid dehydrogenase activity was studied histochemically in the adrenal cortex of ten human male foetuses, ranging in crownrump length from 3.0 cm to 18.3 cm, with the following steroids: 3β-hydroxy-pregn-5-en-20-one (pregnenolone). 3β,17α-dihydroxy-pregn-5-en-20-one (17α-hydroxypregnenolone). 3β-hydroxy-androst-5-en-17-one (DHA). 3β,17β-dihydroxy-androst-5-ene (androstenediol). 3β-sulphoxy-pregn-5-en-20-one (pregnenolone sulphate). 3β-sulphoxy-17α-hydroxy-pregn-5-en-20-one (17α-hydroxy-pregnenolone sulphate) 3β-sulphoxy-androst-5-en-17-one (DHAsulphate). 3β-hydroxy-5α-androstan-17-one (epiandrosterone). After incubation with pregnenolone, 17α-hydroxypregnenolone, DHA and androstenediol a positive histochemical reaction was obtained in the inner part of the »definitive« cortex and throughout the foetal cortex of all adrenals studied. Initially very weak, the reaction became strongly positive about the twelfth week of foetal life. Pregnenolone sulphate and 17α-hydroxypregnenolong sulphate also gave a histochemical reaction in all the adrenals investigated, but DHA sulphate differed significantly from the free steroid by giving a very poor reaction. Formazan deposition followed incubation with epiandrosterone in all adrenals used and this may imply that a δ5 configuration is not necessary for enzyme-substrate binding.

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THE ROLE OF ESTRADIOL IN THE SUSTENTATION OF FETOPLACENTAL SYSTEM

Bulletin physiology and pathology of respiration ◽

10.12737/21458 ◽

2016 ◽

Vol 1 (61) ◽

pp. 75-79

Author(s):

Андриевская ◽

Irina Andrievskaya ◽

Луценко ◽

Mikhail Lutsenko ◽

Довжикова ◽

...

Keyword(s):

The State ◽

Cmv Infection ◽

Histochemical Reaction ◽

Third Trimester ◽

Paraffin Sections ◽

Igg Antibody ◽

The Third ◽

Exchange Processes ◽

Hormonal Dysfunction

The aim of the study is to define the role of abnormalities in the formation of estriol hormones in the development of hormonal dysfunction of fetoplacental system. There were examined 39 placentas from women with exacerbation of chronic cytomegalovirus infection at the third trimester of pregnancy and IgG antibody titer 1:1600 and 30 placentas from women with latent CMV infection and titer 1:400. The activity of androstenedione dehydrogenase was found out with histochemical method on cryostat sections of freshly frozen tissues of placentas by Lloyd’s method. The assessment of intensiveness of histochemical reaction was done with cytophotometric method by Scion program. Estradiol in homogenate of placenta was measured with immune-enzyme method. The quantity of nuclei of placenta syncytiotrophoblast in the state of apoptosis was done with cytophotometric method on paraffin sections stained by ISEL method. By the results of the study the exacerbation of CMV infection at the third trimester of pregnancy leads to the decrease of intensiveness of histochemical reaction in placentas to androstenedione dehydrogenase till 32.1±2.88 pixel/mcm2 (at the latent course it is 54.2±4.31 pixel/mcm2, р<0.001), and in homogenate of placenta to the decrease of estradiol till 18443.2±117.53 pmole/l (at the latent course of the disease it is 28977.7±158.13 pmole/l, p<0.001), on paraffin sections in syncytiotrophoblast by the increase of nuclei number in the state of apoptosis till 5.0±0.03% (at latent course of the disease it is 1.2±0.001%, р<0.001) and vacuole formation, which leads to destructive damages of syncytiotrophoblast cytoplasm. The obtained results prove that the exacerbation of CMV infection at the third trimester of pregnancy causes the decrease of estriol hormones formation, which is accompanied by the damage of morphostructure and exchange processes in cell elements of placenta.

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Effects of the antioestrogen tamoxifen on steroid induced morphological and biochemical changes in the castrated dog prostate

Acta Endocrinologica ◽

10.1530/acta.0.1000462 ◽

1982 ◽

Vol 100 (3) ◽

pp. 462-472 ◽

Cited By ~ 10

Author(s):

P.-J. Funke ◽

U. W. Tunn ◽

Th. Senge ◽

F. Neumann

Keyword(s):

Benign Prostatic Hyperplasia ◽

Biochemical Analysis ◽

Squamous Metaplasia ◽

Combined Treatment ◽

Prostatic Hyperplasia ◽

Biochemical Changes ◽

Weight Increase ◽

Histochemical Reaction ◽

Dna And Rna ◽

Different Types

Abstract. The effect of the antioestrogen tamoxifen (TA) was investigated in different types of steroid-induced benign prostatic hyperplasia (BPH) in the castrated dog by histological, histochemical and biochemical analysis. A 6 months treatment with oestradiol-17β (E2) alone resulted in cystic and stromal hyperplasia and squamous epithelial metaplasia with a striking prostatic weight increase. DNA and RNA content of the total glands increased significantly. The histochemical results and zinc values indicated the loss of normal epithelial function due to metaplatic transformation. The E2 induced cystic and metaplastic hyperplasia was prevented by TA while the stromal proliferation was significantly decreased but not abolished. Biochemical determinations revealed an effect similar to castration. After combined treatment with E2 and 3α-androstanediol (3α-diol) TA completely suppressed squamous metaplasia. A 3α-diol induced glandular proliferation, monitored by a positive histochemical reaction, and significantly elevated zinc, DNA and RNA contents prevailed. A partial stromal stimulation indicates stimulating effects of 3α-diol too on the stroma. The antioestrogenic effects of tamoxifen on experimentally induced BPH mainly manifest at the E2 induced epithelial alterations. The abolishing effects at the stromal level are distinct but not so impressive.

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ACETYL DISULFIDE, (CH3COS)2, AND BIS-(THIOACETOXY) AURATE (I) COMPLEX, Au(CH3COS)2–, HISTOCHEMICAL SUBSTRATES OF UNUSUAL PROPERTIES WITH ACETYLCHOLINESTERASE

Journal of Histochemistry & Cytochemistry ◽

10.1177/16.12.754 ◽

1968 ◽

Vol 16 (12) ◽

pp. 754-764 ◽

Cited By ~ 14

Author(s):

GEORGE B. KOELLE ◽

RICHARD DAVIS ◽

MARTINA DEVLIN

Keyword(s):

Metal Ion ◽

Histochemical Staining ◽

Histochemical Reaction ◽

Black Precipitate ◽

Spontaneous Hydrolysis ◽

Sigmoid Curve ◽

Fine Localization ◽

Thiolacetic Acid ◽

Bell Shaped Curve ◽

Supernatant Solution

The histochemical reaction for acetylcholinesterase (AChE) employing acetyl disulfide ([CH3COS]2, or AcDiS) as substrate and 0.006 M Pb(NO3)2 as capturing agent was characterized by (1) a sigmoid curve for velocity of hydrolysis versus substrate concentration and (2) a red precipitate (presumably [CH3COSS]2Pb) at sites of AChE at the motor endplates (MEP's) and (from spontaneous hydrolysis) in the supernatant solution. With the addition of 0.03 M thiolacetic acid (TA) to the incubation medium, which by itself produced little or no histochemical staining, the foregoing characteristics were changed to (1) a bell-shaped curve, with the peak at 0.003 M AcDiS, and (2) a black precipitate (presumably PbS) at the MEP's and in the supernatant solution; in addition, the velocity of hydrolysis was increased approximately 100-fold. Comparable differences were obtained with AcDiS as substrate when using ionic Au+ or Au(S2O3)2–3 as the capturing agent. Results are explained on the basis that in the absence of free heavy metal ion ( i.e., with the Pb[TA]2 or Au[S2O3]2–3 complex), AcDiS combines with AChE at both the anionic and esteratic sites, whereas, in the presence of Pb2+ or Au+, attachment of the substrate occurs only at the esteratic site of the enzyme. Results similar to the former type were obtained when the bis-(thioacetoxy) aurate (I) complex (Au[CH3COS]2– or Au[TA]2– served as both substrate and capturing agent. With both the AcDiS-Au(S2O3)2–3 and Au (TA)2– methods, extremely fine localization of AChE was obtained at the MEP's by electron microscopy.

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A METHOD FOR PHOTOELECTRIC COMPARISON OF RELATIVE INTENSITIES OF THE HISTOCHEMICAL REACTION OF PHOSPHOMONOESTERASES, PARTICULARLY IN THE ADENOHYPOPHYSIS OF THE GUINEA-PIG

Acta Endocrinologica ◽

10.1530/acta.0.0090161 ◽

1952 ◽

Vol 9 (2) ◽

pp. 161-187 ◽

Cited By ~ 2

Author(s):

LEO ABOLINŠ

Keyword(s):

Guinea Pig ◽

Histochemical Reaction

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HISTOCHEMICAL DISTRIBUTION OF SOME HYDROXYSTEROID DEHYDROGENASES IN THE PLACENTA, FOETAL MEMBRANES AND UTERINE MUCOSA OF THE MOUSE

Journal of Endocrinology ◽

10.1677/joe.0.0400189 ◽

1968 ◽

Vol 40 (2) ◽

pp. 189-NP ◽

Cited By ~ 14

Author(s):

V. BOTTE ◽

S. TRAMONTANA ◽

G. CHIEFFI

Keyword(s):

First Generation ◽

Second Generation ◽

Giant Cells ◽

Yolk Sac ◽

Histochemical Reaction ◽

Hydroxysteroid Dehydrogenases ◽

Foetal Membranes ◽

Uterine Mucosa ◽

Endodermal Cells ◽

Yolk Sac Placenta

SUMMARY The placenta, foetal membranes and uterine mucosa of mice (pregnant for 8–17 days) have been investigated by histochemical methods for NAD-dependent 3β-hydroxysteroid dehydrogenase (3β-HSDH), and for NAD-and NADP-dependent 17α- and 17β-hydroxysteroid dehydrogenases (17α- and 17β-HSDH), 11α- and 11β-hydroxysteroid dehydrogenases (11α- and 11β-HSDH), and 20β-hydroxysteroid dehydrogenase (20β-HSDH). 3β-HSDH was found to be distributed in the trophoblastic giant cells of the first generation with both pregnenolone and DHA as substrates, and in the giant cells of the second generation and of the labyrinth and the endodermal cells of the inverted yolk sac placenta, but only with DHA as substrate. 17α-HSDH and 17β-HSDH, NAD-dependent, were present in both the first and second generation giant cells and in the giant cells of the labyrinth as well as in the endodermal cells of the inverted yolk sac placenta. With NADP as cofactor, 17α-HSDH and 17β-HSDH were weakly positive with all the substrates used in the giant cells of the second generation and of the labyrinth, while NADP-dependent 17β-HSDH was present in the first generation giant cells and in the endodermal cells of the inverted yolk sac placenta but only with oestradiol-17β as substrate. The histochemical reaction for 11α-HSDH, both NAD- and NADP-dependent, was limited to trophoblastic giant cells of the second generation and of the labyrinth; 11β-HSDH, both NAD- and NADP- dependent, was distributed in the giant cells of the second generation and of the labyrinth, the epithelial cells of the uterine mucosa and the decidua basalis. The histochemical reaction for 20β-HSDH, NAD- and NADP-dependent, was weakly positive in the giant cells of the first generation only.

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