Antibodies to enzymes of antioxidant system as a pathogenetic component of anemia in rheumatoid arthritis

2019 ◽  
pp. 96-100
Author(s):  
И.П. Гонтарь ◽  
О.И. Емельянова ◽  
О.А. Русанова ◽  
Л.А. Маслакова ◽  
И.А. Зборовская ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Magnetic Material ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Protein Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Concentration ◽  
Glutaric Aldehyde

Цель исследования - изучение антителообразования к супероксиддисмутазе, глутатионредуктазе, каталазе у больных ревматоидным артритом и выяснение их роли в патогенезе сопутствующей анемии. Методика. Проведено обследование 104 больных ревматоидным артритом с различной степенью активности процесса, формой и характером течения. Диагноз ревматоидного артрита ставился на основании клинико-лабораторного и инструментального обследования больных согласно системе диагностических критериев Американской ревматологической ассоциации, предложенной в 2010 году (ACR / EULAR). Выраженность анемии оценивали по уровню гемоглобина и количеству эритроцитов. Определение уровня антител проводили методом непрямого иммуноферментного анализа [ELISA тест] с использованием иммобилизированных магнитосорбентов, представляющих собой полиакриламидные гранулы, содержащие магнитный материал и перечисленные ферменты в качестве антигена. В качестве антигенов использовались коммерческие отечественные препараты: супероксиддисмутаза из эритроцитов человека (активность 30 Ед/мг), в исследованиях использовали фермент в рабочем разведении по белку - 100 мкг/мл, глутатионредуктаза (активность 340 Ед/мг) - 200 мг/мл по белку, каталаза (активность 380 Ед/мг) - 1,4 мг/мл по белку. Учитывая достаточную относительную молекулярную массу глутатионредуктазы и каталазы, иммобилизацию проводили методом эмульсионной полимеризации в потоке газообразного азота с включением магнитного материала. В связи с небольшой относительной молекулярной массой эритроцитарной фиксации супероксиддисмутазы иммобилизацию фермента проводили путем пришивки его молекулы глютаровым альдегидом к инертной полиакриламидной грануле, содержащей магнитный материал. «Чистые» антитела к ферментам получали с помощью соответствующего антигенного иммуносорбента. Источником специфических иммуноглобулинов служили сыворотки больных ревматоидным артритом с заранее определенным высоким титром антител (экстинция>0,2). После инкубации антигенного иммуносорбента и растворимой формы фермента с полученными антителами был проведен анализ изменения активности энзима. Статистическую обработку результатов проводили с использованием программных пакетов Statistica 6.0, Excel 5.0, Statgraphics 3.0, SPSS 12.0. Результаты. Выявлена зависимость между уровнем антител к супероксиддисмутазе, глутатионредуктазе и каталазе и активностью, течением и формой болезни. Полученные результаты свидетельствуют о повышении антителогенеза к этим ферментам по мере активизации патологического процесса. Выявленное снижение активности энзимов, связанное с выработкой антител к ним, происходит, очевидно, вследствие блокирования специфическими иммуноглобулинами активного центра фермента, являющегося одновременно и антигенной детерминантой. Об участии антител к ферментам в патогенезе анемии у больных ревматоидным артритом свидетельствует обратная корреляция между содержанием антител и уровнем гемоглобина. У больных ревматоидным артритом с умеренно выраженной анемией содержание антител было значимо выше, чем у пациентов без анемии, но ниже, чем у пациентов с тяжелой формой анемии. Заключение. Антитела к супероксиддисмутазе, глутатионредуктазе и каталазе являются одним из патогенетических факторов развития анемии и могут служить критерием тяжести заболевания. The aim was to study formation of antibodies to superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) in patients with rheumatoid arthritis (RA) and to elucidate the role of the antibodies in development of anemia. Methods. The antibodies were determined by enzyme-linked immunosorbent assay (ELISA) using immobilized magnetic sorbents, polyacrylamide granules containing a magnetic substance and the enzymes (SOD, GR, CAT) as antigens. Antibody concentration was expressed in absorbance units. Rheumatoid arthritis was diagnosed based on clinical and instrumental evaluation of patients according to the criteria of the American College of Rheumatology (ACR/EULAR, 2010). Statistical analysis was performed using variation statistics, and results were expressed as mean±SEM. Central tendencies were compared using the Student’s test. Differences were considered statistically significant at p<0.05. ELISA was performed on blood serum from 104 rheumatoid arthritis patients with different disease activity. The following commercial reagents (Russia) were used as antigens: superoxide dismutase from human RBCs (30 U/mg), which was used in the study in a working protein dilution of 100 mcg/ml; glutathione reductase (340 U/mg) which was used in the study at a protein concentration of 200 mg/ml; and catalase (380 U/mg), which was used in the study at a protein concentration of 1.4 mg/ml. Since the relative molecular weights of glutathione reductase and catalase were sufficient, immobilization was performed by emulsion polymerization in a flow of gaseous nitrogen including a magnetic material. Due to a low relative molecular weight of SOD from RBCs this enzyme was immobilized by coupling to an inert polyacrylamide granule containing a magnetic material, using glutaric aldehyde. «Pure» antibodies to the enzymes were obtained using a respective antigen immunosorbent. Specific immunoglobulins were obtained from blood serum of rheumatoid arthritis patients with a known high antibody titer (extinction > 0.2). Enzymatic activity was analyzed following incubation of the antigen immunosorbent and soluble enzyme with the obtained antibodies. Results. Production of antibodies to the studied enzymes increased with increasing severity of the disease. The decrease in enzymatic activity associated with production of respective enzyme antibodies is apparently due to inhibition by specific immunoglobulins of the active center, which is also the antigenic determinant. Conclusion. Antibodies to SOD, GR, and CAT are pathogenetic factors in the development of anemia; they may serve as a criterion for disease severity.

scholarly journals POS0169 FETUIN-A AS A MARKER OF OSTEOPOROSIS AND OSTEOPOROTIC FRACTURES IN PATIENTS WITH RHEUMATOID ARTHRITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 297.2-297
Author(s):  
Y. Akhverdyan ◽  
E. Papichev ◽  
В. Zavodovsky ◽  
L. Seewordova ◽  
J. Polyakova
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Serum ◽  
Bone Mineralization ◽  
Expectant Management ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
High Concentration ◽  
Mineral Density ◽  
Fetuin A ◽  
Apparently Healthy

Background:The main mechanism of the effect of fetuin-A (FeA) on bone metabolism is its ability to bind calcium and proteins of the TGF-β family. It has been proven that the optimal concentration of TGF-β is necessary for the differentiation of bone tissue, and a high concentration inhibits bone mineralization. Thus, adequate osteogenesis is based on a complex balance between FeA and TGF-β levels. It can be assumed that the determination of the FeA level in the blood of patients with rheumatoid arthritis (RA) will help to optimize the diagnosis and predict the severity of osteoporosis (OP).Objectives:to study the possibility of predicting the development of osteoporosis and osteoporetic fractures in patients with RA, depending on the level of FeA in blood serum.Methods:We examined two groups of patients (52 patients with RA complicated by OP, 58 patients with RA without OP) and 30 apparently healthy individuals. The age of the surveyed ranged from 18 to 72 years, the average duration of the disease was 7.53±0.89 years. In both groups, the FeA level was determined by an indirect enzyme-linked immunosorbent assay using a commercial test. Bone mineral density (BMD) was also measured in both groups (Lunar DPX-NT GE).Results:The average FeA level in the group of RA patients was lower than in the group of conventionally healthy individuals (731.21±109.9 μg/ml and 812.9±76.2 μg/ml, respectively; F=13.34; p=0,0004). The normal FeA level was calculated using the formula M±2σ in the group of apparently healthy individuals and ranged from 653.55 μg/ml to 972.19 μg/ml.A decreased level of FeA was found in 20 patients (86.96%) in the group of patients with OP and only in 3 (13.04%) patients with RA who did not suffer from OP (p<0.001). It can be concluded that patients with RA and a low concentration of FeA in the blood serum have a higher risk of developing OP.In the group of patients with normal FeA level, osteoporetic fractures were observed in 12 (13.79%) patients and were absent in 75 (86.21%) patients (p<0.001). Thus, RA patients with normal serum FeA levels have a lower risk of osteoporetic fractures.We also found a positive significant correlation between the level of FeA and BMD in the femoral neck area. In the group of patients with a reduced FeA level (23 people), the mean BMD values were 0.732±0.022 g/cm2, and in the group of patients with a normal FeA level (87 patients) - 0.890±0.014 g/cm2 (p<0.001, F=27.663). The obtained values are in agreement with the literature data on the effect of the serum FeA concentration on the BMD values.Conclusion:We consider it expedient to determine the serum FeA concentration in patients with RA. At a FeA level of 653.55 μg/ml and below, a higher risk of developing OP and osteoporetic fractures can be predicted. In this case, the patient is shown a standard examination for osteoporosis. At values of 653.55 μg/ml and above, a more expectant management of the patient is allowed. Thus, by determining the serum concentration of FeA, it is possible to implement an integrated approach to the patient and to optimize the schemes for the diagnosis of OP in patients with RA.Disclosure of Interests:None declared

scholarly journals POS0451 SERUM FETUIN-A AND VISFATIN LEVELS IN WOMEN WITH RHEUMATOID ARTHRITIS DEPENDING ON DISEASE ACTIVITY

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 455.2-456
Author(s):  
Y. Akhverdyan ◽  
В. Zavodovsky ◽  
E. Papichev ◽  
J. Polyakova ◽  
L. Seewordova
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Serum ◽  
Disease Activity ◽  
Transforming Growth Factor ◽  
Average Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Individuals ◽  
Fetuin A ◽  
Grade Ii

Background:In recent years, the systemic effects of a number of cytokines have been actively studied, in particular, fetuin-A is considered a negative protein of the acute phase response, and visfatin, on the contrary, affects the activation of the cytokine cascade and has a pro-inflammatory effect. Taking into account that women suffer from rheumatoid arthritis (RA) more often, we investigated the levels of fetuin-A and visfatin in the blood serum of females in comparison with a group of healthy individuals and depending on the activity of the disease.Objectives:to study the levels of fetuin-A and visfatin in the blood serum of women suffering from RA, depending on the activity of the diseaseMethods:The study included 110 women with RA and 30 apparently healthy individuals. The inclusion criteria were: a diagnosis of RA verified based on the criteria of the American College of Rheumatology/European Anti-Rheumatic League (ACR/EULAR) 2010. The patients’ age ranged from 18 to 90 years. The control group included 30 conventionally healthy individuals. Serum fetuin-A and visfatin levels were determined by indirect enzyme-linked immunosorbent assay using commercial kits. RA activity was determined by the DAS28-CRP index. Activity 0-I was in 33 (30%) patients, grade II in 67 (60.9%), grade III in 10 (9.09%) patients.Results:The normal level of fetuin-A was calculated using the formula M±2σ in the group of conventionally healthy individuals and ranged from 653.55 to 972.19 μg/ml. In patients with grade 0-I RA activity according to DAS28, the mean serum fetuin-A level was 843.92±130.73 μg/ml, in patients with grade II activity - 742.37±98.85 μg / ml, with III the degree of activity - 663.9±123.7 μg/ml (p<0.001).The average level of visfatin in the blood serum in healthy individuals was 2.43±0.17 ng/ml. The level of normal values of visfatin in healthy individuals, defined as M±2σ, ranged from 0 to 5.07 ng/ml. The average level of visfatin in patients with RA was 6.27±0.18 ng/ml, which is significantly higher than in healthy individuals (p<0.001).In patients with 0-I degree of RA activity according to DAS28, the average level of visfatin in blood serum was 4.94±0.02 ng/ml, in patients with degree II activity - 5.08±0.02 ng/ml, with III degree of activity - 6.82±0.23 ng/ml (p<0.001).Conclusion:Thus, the level of fetuin-A in the blood serum of patients with RA is significantly lower in the case of a high degree of disease activity. The level of visfatin in the blood serum in women with RA is significantly higher in patients with a higher degree of disease activity. Therefore, the concentration values of fetuin-A and visfatin in the blood serum of patients with RA can be used in an integrated assessment of the prognosis of disease activity.References:[1]Inoue K, Ikeda Y, Yamanaka S, et al. Serum fetuinA levels in patients with rheumatoid arthritis [abstract]. Atherosclerosis. 2002;9(Suppl 1):233. doi: 10-1016/s1567-5688(08)70930-9[2]Janssens K, ten Dijke P, Janssens S, et al. Transforming growth factor-beta1 to the bone. Endocrine Reviews. 2005;26(6):743-74. doi:10.1210/er.2004-0001[3]Polyakova J, Korolik O, Papichev E, et al. The role of «new» cytokines in the pathogenesis rheumatoid arthritis. Ann Rheum Dis. 2018; 78(2): 1497Disclosure of Interests:None declared

scholarly journals Antioxidant protection enzyme activity in the blood serum and large intestinal mucosa of rats with prolonged gastric hypochlorhydria and given multiprobiotics

2019 ◽  
Vol 10 (4) ◽  
pp. 438-444
Author(s):  
S. V. Pylypenko ◽  
A. A. Koval
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Catalase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Control Group ◽  
Antioxidant Protection ◽  
Colon Mucosa

The activity of antioxidant protection enzymes in the blood serum and colon mucosa in rats was studied under the conditions of 28-days administration of omeprazole on its own and omeprazole together with multiprobiotics "Symbiter" and "Apibact". Physiological and biochemical study methods were applied. It was found that after omeprazole administration, the activity of superoxide dismutase in the blood serum decreased, and the activity of catalase increased compared to the control. With the co-administration of omeprazole and multiprobiotics, the activity of superoxide dismutase increased compared to the group of rats that received omeprazole only during the same time, but remained less compared to the control group. The content of reduced glutathione in the blood serum of rats after administration of omeprazole decreased, the activity of glutathione peroxidase and glutathione transferase increased, and the activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics, the serum RG content was at the control level, the activity of glutathione reductase exceeded the control values. The activity of glutathione reductase decreased compared to the group receiving omeprazole only. The activity of glutathione reductase increased and did not differ from the control values. In the colon mucosa, superoxide dismutase and catalase activity decreased compared to control. With the combined administration of omeprazole and multiprobiotics, superoxide dismutase and catalase activity increased and even exceeded the control values. With the administration of omeprazole, the reduced glutathione content in the colon mucosa was lower than that in the control. The activity of glutathione peroxidase increased and glutathione transferase and activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics to rats, the reduced glutathione content increased compared to the group of rats administered omeprazole only, and even exceeded that in the control.

scholarly journals Clinical value of blood serum enzymatic activity, proinfl ammatory cytokines and ferritin in rheumatoid arthritis

2019 ◽  
Vol 18 (5) ◽  
pp. 77-83
Author(s):  
M.V. Volkava ◽  
◽  
E.V. Kundzer ◽  
I.I. Generalov ◽  
S.A. Senkovich ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Enzymatic Activity ◽  
Blood Serum ◽  
Clinical Value

scholarly journals Study of activity of enzymes of antioxidant system of blood at HIV infection

2010 ◽  
Vol 56 (5) ◽  
pp. 596-601 ◽  
Author(s):  
V.V. Kostyushov ◽  
I.I. Bokal ◽  
S.A. Petrov
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Hiv Infection ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Oxidative Modification ◽  
Antioxidant Defence ◽  
Activity Of Enzymes

HIV infection is accompanied by activation of lipid peroxidation, oxidative modification of lipoprotein complexes, and a decrease in activity of antioxidant enzymes (superoxide dismutase (SOD), catalase and glutathione peroxidase (GP)) in blood serum. A significant increase of glutathione reductase observed under these conditions is considered as a function of the antioxidant defence. These changes were already seen at early (symptomless) stages of this disease, however, at manifested forms they were more pronounced.

scholarly journals Prooxidative and fluorescence properties of paracetamol during interactions with mitochondria

2011 ◽  
Vol 25 (1) ◽  
pp. 45-51
Author(s):  
Vladimíra Tomečková ◽  
Alena Gajová ◽  
Beáta Veliká ◽  
Lýdia Saxunová ◽  
Zdenka Hertelyová
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Glutathione Peroxidase ◽  
Enzymatic Activity ◽  
Glutathione Reductase ◽  
Mitochondrial Protein ◽  
Fluorescence Properties ◽  
Toxic Dose ◽  
Mitochondrial Functions ◽  
Isolated Liver

This study was carried out to investigate isolated liver mitochondrial functions after paracetamol administration by monitoring of liver mitochondrial fluorescence properties as well as prooxidative properties of paracetamol. Paracetamol was administered to rat (in subtoxic 500 mg·kg−1dose) invivo. The effect of this dose was compared with the subtoxic and toxic dose of paracetamol added to mitochondria invitro(1 and 1.5) mg paracetamol/mg mitochondrial protein. Subtoxic dose of paracetamol invitrodid not change mitochondrial fluorescence, but it significantly decreased mitochondrial fluorescence invivoin comparison with control mitochondrial group. Toxic dose of paracetamol invitrosignificantly decreased mitochondrial fluorescence. The enzymatic activity of superoxide dismutase (SOD) significantly decreased after paracetamol administration invitroand invivo. While both activities of glutathione peroxidase (GPx) and glutathione reductase (GR) significantly increased in dependence upon paracetamol doses. Our experiment showed, that paracetamol participates in formation of free radicals and confirms previous studies, in which paracetamol administration caused elevation of antioxidative enzymes activities in dependence on dose, which is considered therapeutically as subtoxic and toxic.

Treatment with Melittin Induces Apoptosis and Autophagy of Fibroblast-like Synoviocytes in Patients with Rheumatoid Arthritis

2020 ◽  
Vol 21 (8) ◽  
pp. 734-740 ◽  
Author(s):  
Shou-di He ◽  
Ning Tan ◽  
Chen-xia Sun ◽  
Kang-han Liao ◽  
Hui-jun Zhu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Caspase 3 ◽  
Joint Destruction ◽  
Joint Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caspase 9 ◽  
Inflammatory Processes ◽  
Related Proteins ◽  
Local Stimulation ◽  
Fibroblast Like Synoviocytes

Background: Melittin, the major medicinal component of honeybee venom, exerts antiinflammatory, analgesic, and anti-arthritic effects in patients with Rheumatoid Arthritis (RA). RA is an inflammatory autoimmune joint disease that leads to irreversible joint destruction and functional loss. Fibroblast-Like Synoviocytes (FLS) are dominant, special mesenchymal cells characterized by the structure of the synovial intima, playing a crucial role in both the initiation and progression of RA. Objective: In this study, we evaluated the effects of melittin on the viability and apoptosis of FLS isolated from patients with RA. Methods: Cell viability was determined using CCK-8 assays; apoptosis was evaluated by flow cytometry, and the expression levels of apoptosis-related proteins (caspase-3, caspase-9, BAX, and Bcl-2) were also determined. To explore whether melittin alters inflammatory processes in RA-FLS, IL-1β levels were determined using an enzyme-linked immunosorbent assay (ELISA). Furthermore, we performed GFP-LC3 punctate fluorescence dot assays and western blotting (for LC3, ATG5, p62, and Beclin 1) to assess autophagy in RA-FLS. Results: Our results show that melittin can significantly impair viability, promote apoptosis and autophagy, and inhibit IL-1β secretion in RA-FLS. Conclusion: Melittin may be useful in preventing damage to the joints during accidental local stimulation.

scholarly journals POS0441 DEVELOPMENT OF RHEUMATOID ARTHRITIS AMONG ANTI-CITRULLINATED PROTEIN ANTIBODIES POSITIVE ASYMPTOMATIC INDIVIDUALS: A PROSPECTIVE OBSERVATIONAL STUDY

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 449.1-449
Author(s):  
S. Mizuki ◽  
K. Horie ◽  
K. Imabayashi ◽  
K. Mishima ◽  
K. Oryoji
Keyword(s):  
Rheumatoid Arthritis ◽  
Risk Factors ◽  
Observational Study ◽  
Rheumatoid Factor ◽  
Prospective Observational Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Healthy Population ◽  
Asymptomatic Individuals ◽  
Citrullinated Protein

Background:In the idividuals with genetic and enviromental risk factors, immune events at mucosal surfaces occur and may precede systemic autoimmunity. Anti-citrullinated protein antibodies (ACPA) are present in the serum for an average of 3-5 years prior to the onset of rheumatoid arthritis (RA) during an asymptomatic period. In ACPA-positivite individuals, the additional presence of RA-related risk factors appears to add significant power for the development of RA. To date, there have been few reports in which clinical courses of ACPA-positive asymptomatic individuals were investigated prospectively.Objectives:To observe the clinical time course of ACPA-positive healthy population for the development of RA.Methods:Healthy volunteers without joint pain or stiffness, who attended the comprehensive health screening of our hospital, were enrolled in this prospective observational study. The serum ACPA levels were quantified by Ig-G anti-cyclic citrullinated peptide enzyme-linked immunosorbent assay with levels > 4.4 U/mL considered positive. ACPA-positive subjects were followed by rheumatologists of our department clinically or a questionnaire sent by mail for screening to detect arthritis.Results:5,971 healthy individuals without joint symptons were included. Ninty-two (1.5%) were positive for ACPA. Of these, 19 (20.7%) developed RA and two were suspected as RA by mail questionnaire. Their average age were 58-years, and women were 68%. The average duration between the date of serum sampling and diagnosis was 10.7 months. ACPA-positive individuals who developed to RA had higher serum ACPA and Ig-M rheumatoid factor levels than ACPA-positive individuals who did not (P value by Mann-Whitney U test: 0.002, 0.005, respectively).Conclusion:Among ACPA-positive asymptomatic individuals, 20% developed RA. The higher titer of ACPA and Ig-M rheumatoid factor levels are risk factors for devoloping RA.Disclosure of Interests:None declared

scholarly journals Mesenchymal stem cell-originated exosomal lncRNA HAND2-AS1 impairs rheumatoid arthritis fibroblast-like synoviocyte activation through miR-143-3p/TNFAIP3/NF-κB pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Yuhua Su ◽  
Yajing Liu ◽  
Chao Ma ◽  
Chunxiao Guan ◽  
Xiufen Ma ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Tumor Necrosis Factor ◽  
Western Blot ◽  
Tumor Necrosis ◽  
Cell Counting ◽  
Biological Behavior ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Interaction ◽  
Necrosis Factor

Abstract Background Long non-coding RNA heart and neural crest derivatives expressed 2-antisense RNA 1 (HAND2-AS1) was found to be elevated in rheumatoid arthritis (RA) fibroblast-like synoviocytes (RA-FLSs). However, whether HAND2-AS1 functions as an exosomal lncRNA related to mesenchymal stem cells (MSCs) in RA progression is unknown. Methods The expression of HAND2-AS1, microRNA (miR)-143-3p, and tumor necrosis factor alpha-inducible protein 3 (TNFAIP3) was detected using quantitative real-time polymerase chain reaction and Western blot. Cell proliferation, apoptosis, migration, and invasion were detected using cell counting kit-8, flow cytometry, and wound healing and transwell assays. The levels of tumor necrosis factor-α (TNF-α) and interleukins (IL)-6 were analyzed using enzyme-linked immunosorbent assay. The level of phosphorylated-p65 was examined by Western blot. The binding interaction between miR-143-3p and HAND2-AS1 or TNFAIP3 was confirmed by the dual-luciferase reporter and RIP assays. Exosomes were isolated by ultracentrifugation and qualified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blot. Results HAND2-AS1 was lowly expressed in RA synovial tissues, and HAND2-AS1 re-expression suppressed the proliferation, motility, and inflammation and triggered the apoptosis in RA-FLSs via the inactivation of NF-κB pathway. Mechanistically, HAND2-AS1 directly sponged miR-143-3p and positively regulated TNFAIP3 expression, the target of miR-143-3p. Moreover, the effects of HAND2-AS1 on RA-FLSs were partially attenuated by miR-143-3p upregulation or TNFAIP3 knockdown. HAND2-AS1 could be packaged into hMSC-derived exosomes and absorbed by RA-FLSs, and human MSC-derived exosomal HAND2-AS1 also repressed above malignant biological behavior of RA-FLSs. Conclusion MSC-derived exosomes participated in the intercellular transfer of HAND2-AS1 and suppressed the activation of RA-FLSs via miR-143-3p/TNFAIP3/NF-κB pathway, which provided a novel insight into the pathogenesis and treatment of RA.

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