Study of biomarkers for damage of renal tissue in patients with oxalate urolithiasis after transurethral contact ureterolithotripsy

Выявление маркеров патологии почек и введение биохимических индексов, объективно отражающих развитие патологического процесса при мочекаменной болезни, может существенно облегчить диагностику данного заболевания и способствовать выработке правильного и адекватного лечения оксалатного уролитиаза. Известно, что при мочекаменной болезни обнаруживается увеличение продуктов липопероксидации. В таком случае, определение метаболитов перекисного окисления липидов в сыворотке крови может служить одним из критериев прогноза и течения различных форм данного заболевания. Методы. Проведено исследование концентрации липокалина (NGAL) в моче, малонового диальдегида (МДА) и диеновых конъюгатов (ДК) в плазме крови, а также активность ферментов каталазы и глутатионпероксидазы (ГПО) у больных оксалатным уролитиазом, после трансуретральной контактной уретеролитотрипсии (КУЛТ). В исследование включены 72 пациента - у 42 человек выполнена КУЛТ, и 30 пациентов с ранее подтвержденный оксалатный уролитиазом, без оперативного лечения. Контролем служили 10 здоровых испытуемых. Был осуществлен забор крови и мочи пациентов, предварительно разделенных на группы в зависимости от стадии лечения. Результаты. Обнаружено достоверное повышение содержания МДА и ДК, также увеличение активности ГПО во время операции и в первые послеоперационные сутки в сравнении с контрольной группой. Возрастание активности каталазы и увеличение содержания стресс-пептида NGAL в моче выявлено на всех этапах заболевания. Заключение. Активация ПОЛ и усиление каталазной активности можно рассматривать в качестве дополнительного критерия оценки степени антиоксидантной защиты у больных с оксалатным уролитиазом. Identification of markers for renal pathology and implementation of biochemical indices objectively reflecting the development of pathological process in urolithiasis disease would significantly facilitate detection of this disease and contribute to development of proper and adequate treatments in oxalic urolithiasis. Increased lipid peroxidation products are known to be present in urolithiasis. Therefore, detection of lipid peroxidation metabolites in serum could serve as a criterion for course and outcome of various forms of this disease. Materials and methods. Concentrations of urinary lipocalin (NGAL), plasma malonic dialdehyde (MDA) and diene conjugates (DC), and activities of catalase and glutathione peroxidase (GPO) were measured in patients with oxalate urolithiasis after transurethral contact ureterolithotrization (CULT). The study included 72 patients; 42 of them underwent CULT and 30 patients had documented oxalate urolithiasis without surgical treatment. Control group consisted of 10 healthy subjects. Patients were divided into groups based of the stage of treatment, and blood samples were collected. Results. Concentrations of MDA and DK and GPO activity were increased during the surgery and first postoperative days compared to the control group. Catalase activity and urinary concentration of the NGAL stress peptide were increased at all stages of the disease. Conclusion: Activation of lipid peroxidation and catalase can be considered as an additional criterion for evaluating the degree of antioxidant defense in patients with oxalate urolithiasis.

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Features of disorders of the system of cytokines and lipid peroxidation in acute ulcerative gastroduodenal hemorrhages

Kazan medical journal ◽

10.17750/kmj2017-686 ◽

2017 ◽

Vol 98 (5) ◽

pp. 686-691 ◽

Cited By ~ 1

Author(s):

D N Gadzhiev ◽

V A Allakhverdiev ◽

N D Gadzhiev

Keyword(s):

Lipid Peroxidation ◽

Interleukin 6 ◽

Antioxidant Defense ◽

Reduced Glutathione ◽

Malonic Dialdehyde ◽

Interferon Γ ◽

Diene Conjugates ◽

Control Value ◽

Factor Α ◽

Necrosis Factor

Aim. Investigation of the local and systemic levels of some pro- and anti-inflammatory cytokines as well as the system of lipid peroxidation - antioxidant defense of the organism in acute ulcerative gastroduodenal hemorrhages, taking into account the severity of the blood loss. Methods. We performed the investigation of cytokines in the blood (tumor necrosis factor α, interferon γ, interleukin-1, -2, -4, -6, -8, -10) and parameters of lipoperoxidation (in plasma - diene conjugates, malonic dialdehyde, catalase; in erythrocytes - reduced glutathione, glutathione peroxidase, total antioxidant activity), interleukin-6 in gastric content and urine, diene conjugates and malonic dialdehyde in biopsy samples from the ulcer edges from patients with acute ulcerative gastroduodenal bleeding. The blood serum of 156 patients with mild (54), moderate (51) and severe (51) bleeding was studied. Interleukin-6 in gastric contents was determined in 37 patients, diene conjugates and malonic dialdehyde in biopsy samples taken from periulcerous zone - in 20 patients. Results. Patients had a statistically significant increase of serum levels of tumor necrosis factor α, interferon γ, interleukins-1, -2, -4, -6, -8, and decrease of interleukin-10 concentration in comparison with the control group. The level of interleukin-6 in gastric juice was 37.5% lower, and in urine - 8.2 times higher than the control value. In plasma and biopsy samples, the content of diene conjugates and malonic dialdehyde was statistically significantly higher, in erythrocytes the level of reduced glutathione was lower than the control value. The depth of imbalance in the system of cytokines and lipid peroxidation - antioxidant defense increased with increasing of blood loss severity. Conclusion. The relationship between cytokine production and lipid peroxidation indices is established, which confirms the role of local and systemic disorders of cytokine and antioxidant status in the occurrence of ulcer bleeding.

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THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN WOMEN WITH PRIMARY VARICOSE VEINS OF THE PELVIC DEPENDING ON THE STAGE OF THE DISEASE

Annals of the Russian academy of medical sciences ◽

10.15690/vramn1005 ◽

2018 ◽

Vol 73 (4) ◽

pp. 229-235 ◽

Cited By ~ 2

Author(s):

Lyubov’ I. Kolesnikova, ◽

Andrey A. Semendyaev ◽

Dmitriy A. Stupin ◽

Marina A. Darenskaya ◽

[Lyudmila A. Grebenkina ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Defense ◽

Varicose Veins ◽

Reproductive Age ◽

Background Information ◽

Control Group ◽

Maximum Increase ◽

Diene Conjugates ◽

Primary Varicose Veins ◽

Peroxidation Processes

Background: Information about involvement of general nonspecific reactions, in particular lipid peroxidation processes, in the progression of varicose veins of the pelvic (VVP) in women is still too scarce. Aims: To study the intensity of processes of lipid peroxidation and the activity of components of the antioxidant system in women with primary varicose veins of the pelvic at different stages of the disease. Materials and methods: 167 women of reproductive age were examined ― 137 with VVP and 30 made up a control group. All patients with VVP were divided into 3 groups depending on stages of the disease. Spectrophotometric and fluorometric methods of investigation were used. The study was conducted during 2012−2017. Results: It was revealed that the level of primary products of lipid peroxidation, diene conjugates, increased statistically significantly according to the stage of the disease by 1.25 times (in the 1st stage), 1.51 times (in the II stage) and 1.59 times (in the III stage) values. Changes in the content of final TBA-active products showed similar changes-an increase in the mean values for all stages of the disease relative to control (in 1.24, 1.17, and 1.77 times, respectively). Activity of glutathione peroxidase increased in stage 2 of VVP (1.19 times), with the maximum increase in stage III (1.42 times); activity of glutathione-S-transferase increased 1.18 times in the II stage of the disease. The concentration of GSH in the clinical groups was characterized by lower values with respect to the control (by 1.22 times in the 1st stage, in 1.64 times in the II stage), with the maximum decrease of this parameter in the III stage of VVP (3.67 times). The level of catalase activity increased in the I stage of VVP ― by 1.18 times and decreased in the III stage ― by 1.14 times with respect to the control. The activity of SOD showed similar changes with catalase ― in the form of increased activity at the 1st stage (1.35 times higher) and decreased values for II (1.35 times lower) and III (1.65 times lower) for the stages of VVP to the values of control. Conclusions: At progression of primary VVP in women (from the initial stage to the 3rd stage of the disease), there is an increase in imbalance in the lipid peroxidation − antioxidant defense system. Moreover, if the compensatory increase in activity of antioxidant enzymes is registered at stage 1 of the disease, then the most of the antioxidant defense factors decreases as relative to control values, and the initial stages of the disease.

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Assessment of the state of lipid peroxidation and antioxidant system in children with nephrotic form of glomerulonephritis

Kazan medical journal ◽

10.17750/kmj2017-370 ◽

2017 ◽

Vol 98 (3) ◽

pp. 370-375

Author(s):

R O Beglyarov

Keyword(s):

Lipid Peroxidation ◽

Nephrotic Syndrome ◽

Blood Plasma ◽

Antioxidant System ◽

Malonic Dialdehyde ◽

Control Group ◽

Chronic Glomerulonephritis ◽

Diene Conjugates ◽

Control Value ◽

In Blood Plasma

Aim. Study of the activity of lipid peroxidation and state of antioxidant system in children with a nephrotic form of chronic glomerulonephritis. Methods. 104 children with a nephrotic form of glomerulonephritis were examined. The average age of children was 10.18±4.03 years. 46.2% of children were in remission, 32.7% of children had 1st degree of activity, 14.4% had 2nd degree, and 6.7% had 3d degree of nephrotic syndrome. Control group included 30 children without chronic glomerulonephritis. The levels of diene conjugates, malonic dialdehyde, catalase, reduced glutathione, glutathione peroxidase, and glutathione reductase in blood plasma and erythrocytes were determined. Results. In children with chronic glomerulonephritis the concentration of diene conjugates and malonic dialdehyde was elevated in blood plasma and erythrocytes. In erythrocytes their concentration was statistically significantly higher at all degrees of activity of nephrotic syndrome than in control group. The level of catalase in patients’ blood plasma in comparison with the control value was reduced by 16.6%, in erythrocyte mass - by 25.9% (p

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Typical relationships between the indexes of lipid peroxidation/ antioxidant protection and immune regulation in the patients with chronic atrophic gastritis and stomach cancer

Medical Immunology (Russia) ◽

10.15789/1563-0625-trb-2032 ◽

2020 ◽

Vol 22 (5) ◽

pp. 897-906

Author(s):

O. V. Smirnova ◽

V. V. Tsukanov ◽

A. A. Sinyakov ◽

O. L. Moskalenko ◽

N. G. Elmanova ◽

...

Keyword(s):

Gastric Cancer ◽

Lipid Peroxidation ◽

Atrophic Gastritis ◽

Normal Distribution ◽

Antioxidant Defense ◽

Malonic Dialdehyde ◽

Chronic Atrophic Gastritis ◽

The Body ◽

Control Group ◽

Antioxidant Protection

Chronic atrophic gastritis and gastric cancer represent distinct steps of one pathogenic process. The risk of developing cancer of the stomach is directly proportional to the degree of atrophic changes simultaneously detected in antral segment and in the body of the stomach. The role of immune system in transformation of precancerous diseases into cancer is beyond doubt. During development of the malignant disease, the changes in lipid peroxidation systems – antioxidant defense become significant and contribute to the progression of the tumor and the development of metastases. A simultaneous study of lipid peroxidation and antioxidant defense indices along with phagocytic activity will allow us to evaluate relative contribution of these processes to development of chronic atrophic gastritis and gastric cancer. Purpose of the present study was to assess correlations between the lipid peroxidation indices, i.e. antioxidant protection, and chemiluminescent activity of neutrophilic granulocytes and monocytes in chronic atrophic gastritis and gastric cancer. Forty patients with chronic gastritis, 22 patients with chronic atrophic gastritis and 40 patients with gastric cancer were examined. The control group consisted of 50 practically healthy age-matched volunteers. Evaluation of spontaneous and induced production of reactive oxygen species by neutrophils and monocytes was carried out by chemiluminescent analysis. The parameters of lipid peroxidation/ antioxidant protection were determined by spectrophotometric methods. Statistical data processing was carried out using the Statistica v. 8.0 program (StatSoft Inc., USA). The normal distribution of indices was tested using the Kolmogorov–Smirnov method (adjusted by Lillefors). Quantitative indicators, given the normal distribution, were described using the median (Me) and interquartile scatter (Q0.25-Q0.75). To study statistical significance of differences between quantitative characteristics, the Mann–Whitney test was used. To study strength of relationships of these indicators, the Pearson rank correlation coefficient (r) was calculated. The critical significance level (p) when testing statistical hypotheses was taken equal to 0.05. Correlation analysis showed that the weight of positive correlations increases in patients with chronic atrophic gastritis, and it decreases in patients with gastric cancer, the strength of the correlation dependence and new relationships appear between chemiluminescent activity of neutrophils and monocytes in a spontaneous and induced state, and the amounts of malonic dialdehyde, enzyme activities of superoxide dismutase and catalase. In chronic atrophic gastritis and gastric cancer we have established the features of correlation patterns between lipid peroxidation/antioxidant protection indices, and activity of neutrophils and monocytes.

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Antioxidant status of piglets under nitrate-nitrite load and the action of corrective factors

Ukrainian Journal of Veterinary and Agricultural Sciences ◽

10.32718/ujvas3-2.09 ◽

2020 ◽

Vol 3 (2) ◽

pp. 53-59

Author(s):

K. Y. Leskiv ◽

B. V. Gutyj ◽

D. F. Gufriy ◽

V. I. Khalak ◽

O. G. Demchuk

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Control Group ◽

Lipid Hydroperoxides ◽

Defense System ◽

Diene Conjugates ◽

Peroxidation Processes

The article presents the results of research on the state of the antioxidant defense system of piglets under nitrate-nitrite toxicosis and the action of corrective factors (methionine, phenarone and methyphene). Nitrate-nitrite load in piglets caused inhibition of the activity of enzymes of the antioxidant defense system, that indicates a decrease in the activity of superoxide dismutase, catalase and glutathione peroxidase in the serum of testing animals. The lowest activity of antioxidant enzymes under chronic nitrate-nitrite toxicosis was on the 60th day of the experiment in the serum of piglets of the control group, where accordingly the activity of superoxide dismutase decreased by 14 %, catalase activity – by 18 %, glutathione peroxidase activity – by 12 %. Lipid peroxidation processes are activated in the blood of animals, namely the level of lipid hydroperoxides and diene conjugates increases under the conditions of experimental nitrate-nitrite load. It should be noted that the highest level of lipid peroxidation products in the serum of piglets with chronic nitrate-nitrite toxicosis was on the 60 th day of the testing, and accordingly the content of lipid hydroperoxides increased by 67 % and diene conjugates – by 33 %. Administration of methionine, phenarone and methyphene under nitrate load contributed to the activation of the antioxidant defense system compared to animals in the control group. The use of antioxidant drugs in sick piglets also contributed to the inhibition of lipid peroxidation, as indicated by a decrease in the content of lipid hydroperoxides and diene conjugates in the serum of experimental group. More pronounced antioxidant effect was shown by administrating methyphene. The mechanism of action of phenarone and methyphene is associated with the direct effect of its components on the inhibition of the absorption of metabolites that have a toxic effect on cell membranes of animals. As a result of the adsorbing action of zeolite, which is part of the drugs, in the digestive tract there is a decrease in the concentration of substances that can be substrates for lipid peroxidation, as well as removal of toxic metabolites in the blood, which are prooxidants. This process occurs by osmosis and diffusion of these substances through the capillaries of the microvilli of the small intestine and their subsequent fixation on the sorbent granules.

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Laboratory Study: Lipid Peroxidation and Antioxidant Defense Mechanisms in Rat Renal Tissue After Daunorubicin Administration

Renal Failure ◽

10.3109/08860229609047675 ◽

1996 ◽

Vol 18 (4) ◽

pp. 537-543 ◽

Cited By ~ 4

Author(s):

C. Dioudis ◽

D. Grekas ◽

G. Papageorgiou ◽

S. Iliadis ◽

N. Botsoglou ◽

...

Keyword(s):

Lipid Peroxidation ◽

Laboratory Study ◽

Defense Mechanisms ◽

Antioxidant Defense ◽

Renal Tissue

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Effect of 12 Months of Exercise with Stachys Lavandulifolia Consumption on anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women with Syndrome Metabolic

10.21203/rs.3.rs-129277/v1 ◽

2020 ◽

Author(s):

Ali osali ◽

Alireza Rostami

Keyword(s):

Metabolic Syndrome ◽

Lipid Peroxidation ◽

Aerobic Exercise ◽

Antioxidant Defense ◽

Training Group ◽

Serum Levels ◽

Control Group ◽

Negative Effects ◽

Beneficial Effects ◽

Before And After

Abstract BackgroundThe purpose of this study was to investigate the effect of 12 months of aerobic exercise combining stachys lavandulifolia (S. lavandulifolia) consumption on anxiety, Metabolic Syndrome profiles and antioxidant defense (Glutathione) and lipid peroxidation (Malondialdehyde) in 50-65 years old women with syndrome metabolic.Methods48 women with syndrome Metabolic were randomly divided into four groups: exercise (n=12), exercise+S. lavandulifolia (n=12), S. lavandulifolia (n=12) and control group (n=12). S. lavandulifolia groups consumed 3 g aerial parts of S. lavandulifolia daily. Training groups performed an exercise protocol of aerobic exercise for 12 months (three sessions per week). Blood samples were obtained before and after training period for antioxidant indicators and lipid degradation measurement. Also, Beck anxiety questionnaire used for evaluating levels of anxiety. T-test and one-way analysis of variance were used for the evaluation of within-group and between-group differences, respectively.ResultsA significant increase was observed in serum levels of Malondialdehyde (P =0.004), Catalase indexes (Pvalue= 0.01), and Glutathione (P=0.001) in the training group and S. lavandulifolia groups after 12 months. Body weight, BMI, and SBP and Anexiety was decreased significantly greater in exercise +S. lavandulifolia group compared to control, exercise and S. lavandulifolia groups (P=0.001)ConclusionAnxiolytic effect and Anti-Oxidative Stress Activity was seen, so taking S. lavandulifolia along with exercises may have beneficial effects on reinforcement the antioxidant system and prevention of anxiety and The negative effects of indicators related to cardiovascular disease in women with metabolic syndrome.

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Analysis of the relationship between hemoglobin-oxygen affinity and lipid peroxidation during fever.

Acta Biochimica Polonica ◽

10.18388/abp.1995_4670 ◽

1995 ◽

Vol 42 (1) ◽

pp. 69-74 ◽

Cited By ~ 6

Author(s):

M V Borisiuk ◽

V V Zinchuk

Keyword(s):

Lipid Peroxidation ◽

Malonic Dialdehyde ◽

Oxygen Affinity ◽

Alpha Tocopherol ◽

Diene Conjugates ◽

Tocopherol Concentration ◽

Hemoglobin Oxygen Affinity ◽

The Relationship ◽

Free Radical Lipid Oxidation ◽

Oxyhemoglobin Dissociation

Endogenous hyperthermia was induced in rabbits by i.v. pyrogenal administration. Hemoglobin-oxygen affinity and parameters of free radical lipid oxidation in plasma and red blood cells were measured. The content of diene conjugates, malonic dialdehyde and Schiff bases were determined at a pyrogenal dose of 4 minimal pyrogenic doses/kg, and iron-initiated chemiluminescence, catalase activity and alpha-tocopherol concentration were determined at 6 minimal pyrogenic doses/kg. A rightward shift of the real oxyhemoglobin dissociation curve and activation of lipid peroxidation were observed. Relationships between the parameters measured were analyzed. Decreased hemoglobin-oxygen affinity is considered to be a possible mechanism of activation of free radicals during fever.

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On the Opportunities of Using the Indices of Lipid Peroxidation – Antioxidant Defense System in the Diagnostics of Varicose Veins of Small Pelvis in Women

Acta biomedica scientifica ◽

10.29413/abs.2020-5.1.2 ◽

2020 ◽

Vol 5 (1) ◽

pp. 14-20

Author(s):

L. I. Kolesnikova ◽

A. A. Semendyaev ◽

D. A. Stupin ◽

M. A. Darenskaya ◽

L. A. Grebenkina ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Defense ◽

Varicose Veins ◽

Antioxidant Defense System ◽

Pathological Process ◽

Moderate Degree ◽

Defense System ◽

Mild Degree ◽

Severe Degree ◽

Degree Of Severity

Background: The prevalence of varicose veins of small pelvic veins in women of reproductive age varies widely – from 5.4 to 80 %, due to the low specificity and low sensitivity of clinical diagnostic techniques, and the absence of biomarkers that can be used to identify the formation and progression of varicose veins of small pelvic veins in women.Aims: To identify changes in the levels of parameters of the system “lipid peroxidation – antioxidant protection” with the development of varicose veins of the pelvis in women, as well as the possibility of their diagnostic use.Materials and methods: We examined 200 women with clinical signs of varicose veins of small pelvis. Control group included 30 women without any pathology of venous system. Treatment group included 137 women with varicose veins of small pelvis: with mild degree of severity – 39 women, with moderate degree of severity – 65 women, with severe degree of severity – 33 women. We performed comparative analysis of lipid peroxidation – antioxidant defense system in women with and without varicose veins of small pelvis taking into account the severity degrees of pathological process.Results: We obtained the most informative indices for lipid peroxidation – antioxidant defense processes in blood of women with varicose veins of small pelvis: concentration of diene conjugate (DC) for the mild degree of severity of pathological process, lipid hydroperoxide (LHP), DC and malonic dialdehyde (MDA) – for the moderate and severe degree of severity, and also changes in the activity of antioxidant enzymes with increase of the severity of the disease. Assessment of the level of catalase (Cat) and glutathione peroxidase (GP) showed their highest informative value at the mild degree of severity. Concentrations of glutathione reductase (GR), glutathione S-transferase (GST) and reduced glutathione (GSH) are more statistically significant markers for severe forms of pathological process.Conclusions: Our research showed the relevance and advisability of the studying the levels of lipid peroxidation – antioxidant defense indices in women with varicose veins of small pelvis. We registered the highest diagnostic value of DC, Cat and GP in the beginning of the disease and of LHP, DC, MDA, GR, GST and GSH – at severe forms of the pathological process. We obtained reference values for DC, MDA, Cat, SOD and GP concentration that can be considered as the predictors of the development of varicose veins of small pelvis.

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Impact of lead acetate and sodium and potassium stearates on lipid peroxidation processes in the body of experimental animals

Hygiene and Sanitation ◽

10.47470/0016-9900-2021-100-4-406-410 ◽

2021 ◽

Vol 100 (4) ◽

pp. 406-410

Author(s):

Olha Ye. Fedoriv ◽

Alexandra Ye. Kopach ◽

Nataliia A. Melnyk

Keyword(s):

Lipid Peroxidation ◽

Drinking Water ◽

Lead Acetate ◽

Sodium Stearate ◽

The Body ◽

Female Rats ◽

White Female ◽

Control Group ◽

Diene Conjugates ◽

Group 2

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc. Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD50. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates. Results. The administration of 1/2 acetate LD50 to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate. Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD50, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.

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