scholarly journals GC-MS Analysis, Phytochemical Screening and Biological Activity of Bauhinia tomentosa (Linn.)

2021 ◽  
Vol 33 (11) ◽  
pp. 2641-2644
Author(s):  
R. Mini ◽  
V. Prabhu ◽  
K. Vimaladevi ◽  
J. Sowmiya ◽  
G. Sibi
Keyword(s):  
Biological Activity ◽  
Methanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Tetradecanoic Acid ◽  
Ms Analysis ◽  
Ic50 Value ◽  
Gas Chromatography Mass Spectroscopy ◽  
Minor Compounds

Present study aimed to evaluate the chemical composition and biological activity for methanolic extract of Bauhinia tomentosa (Linn.) leaves grown in Western Ghats region of South India. The preliminary phytochemical screening tests revealed the presence of steroids, alkaloids, terpenoids, flavonoids, glycosides and phenolic compounds in the leaf extract. A total of 19 compounds were identified through gas chromatography-mass spectroscopy (GC-MS) analysis of methanolic extract of B. tomentosa. The major compounds identified were phytol (23.96%), n-hexadecanoic acid (11.62%), squalene (8.85%) and the minor compounds are trans-bis(2-methylpropyl)-4,6-dioxane(0.13%), dihydro-cis-α-copaene-8-ol (0.14%), tetradecanoic acid (0.81%), respectively. Antibacterial activity of the extract showed the zone of inhibition 18 mm at 200 μg/mL against S. aureus, followed by 15 and 16 mm against S. anginosus, K. pneumoniae at 200 μg/mL, respectively. Antioxidant activity of methanolic extract of B. tomentosa leaves showed the maximum IC50 value with 75.07 % of scavenging activity at the concentration of 5 μg/mL.

scholarly journals Phytochemical screening and GC MS analysis of methanolic extract of Abelmoschus moschatus

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3049-3052
Author(s):  
Rani Sebastian ◽  
Jayakar B ◽  
Gomathi V
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Cytoprotective Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Acetate Methyl ◽  
Study Results ◽  
Gas Chromatography Mass Spectroscopy ◽  
Vinyl Phenol

The study was designed to evaluate the phytoconstituents present in the methanolic extract of aerial parts of Abelmoschus moschatus. The qualitative phytochemical screening of different extracts of aerial parts revealed the presence of some bioactive compounds. GC – MS analysis was performed using Shimadzu Gas chromatography-mass spectroscopy (Model Number: QP2010S) instrument. GC-MS detection of phytoconstituents was done by computer evaluation of mass spectra of samples through National Institute Standard and Technology (NIST II) and WILEY 8 library. GC – MS analysis detected the presence of 14 compounds. GC – MS profile of the methanolic extract revealed the presence of megastigmatrienone, phytol, loliolide, farnesyl acetate, methyl linoleates, gamma-sitosterol, cis, cis, cis-7,10,13-Hexadecatrienal, thymine, pyranone, coumarin, 2 – methoxy 4 – vinyl phenol, guanosine, chinasaure and 3- cyclopentyl propionic acid 2 dimethyl aminoethyl ester. The current study suggests that methanolic extracts of aerial parts of Abelmoschus moschatus contain phytoconstituents with antioxidant and cytoprotective activity. The study results will pave a way for the production of therapeutic agents which can be used for the treatment of various diseases.

Preliminary phytochemical screening and GC-MS analysis of methanolic extract of Turnera subulata Smith (Passifloraceae)

2017 ◽  
Vol 6 (3) ◽  
pp. 174-177
Author(s):  
◽  
S Vinotha ◽  
A Vijayan ◽  
Anand Gideon V ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Plant Leaf ◽  
Ms Analysis ◽  
Phytochemical Profile ◽  
Gas Chromatography Mass Spectroscopy ◽  
Plant Leaf Extract

The investigation was carried out to determine the possible phytochemical constituents from aqueous, methanol and chloroform extracts of Turnera subulata leaf extracts. Among the phytochemical screening of these extracts, Methanolic extract showed that the leaf was rich in alkaloids, flavonoids, glycosides, phenols, saponins and quinones. The chemical composition of the plant leaf extract of T. subulata was investigated using Gas Chromatography – Mass Spectroscopy (Agilent-7890A GC instrument coupled with MS-5975) and NIST-MS library. GC-MS analysis of T. subulata plant leaf extract, revealed the existence of the GC-MS chromatogram of the major peaks presented in methanolic extract like Methyl 8,11,14-heptadecatrienoate (23.244%), Pentadecanoic acid, 14-methyl-,methyl ester (8.654%), n-Hexadecanoic acid (8.654%), 4H-Pyran4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.598%), 1b,4a-Epoxy-2H-cyclopenta[3,4] cyclopropa[8,9]cycloundec[1,2-b] oxiren-5(1aH)-one(5.400%), 3,7,11,15-Tetramethyl-2-hexadecen-1- ol(5.400%), etc. From this study it is obvious that T. subulata leaf extract contains many biologically active compounds and also it gives a detailed insight about the phytochemical profile which could be exploited for the development of plant based drug.

Identification of the Phytoconstituents in Methanolic Extract of Adhatoda vasica L. Leaves by GC-MS Analysis and its Antioxidant Activity

2021 ◽  
Author(s):  
Nausheen Khan ◽  
Abdul Qadir ◽  
Musarrat Husain Warsi ◽  
Amena Ali ◽  
Abu Tahir ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin E ◽  
Methanolic Extract ◽  
Tetradecanoic Acid ◽  
Adhatoda Vasica ◽  
Ms Analysis ◽  
Dpph Method ◽  
Gas Chromatography Mass Spectroscopy ◽  
Identification And Characterization

Abstract Background Adhatoda vasica L. is a medicinal plant, also known as Malabar nut in English, belongs to family Acanthaceae. It has been used traditionally to treat respiratory disorders like severe cough, cold, chronic bronchitis, asthma, tuberculosis, and other illnesses. The multifunctional range of bioactives found in it has piqued the interest of pharmaceutical companies, who are looking for more evidence-based ways to develop new formulations. Method Methanolic extract of Adhatoda vasica L. leaves (MEAV) was analysed by hyphenated gas chromatography-mass spectroscopy for identification and characterization of its bioactives and traditional therapeutic claim. Widely anticipated DPPH method was used to determine the antioxidant activity of MEAV. Results The major compounds revealed in MEAV leaves are 1,3,5-triazine-2,4,6-triamine (3.06%); 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (5.35%); 5-hydroxymethylfurfural (16.82%); 2-butylphenol (6.85%); 3,4-dihydroxy-5-methyl-dihydro-furan-2-on (2.5%); 2(OR 3)-(1,1-dimethylethyl)-4-methoxyphenol (3.52%); megastigmatrienone 3 (1.02%); tetradecanoic acid (1.52%); vomifoliol (0.58%); oxalic acid, cyclobutylhexyl ester (6.03%); hexadecanoic acid (6.06%); 4-ethyl-2-oxo-2,5,6,7-tetrahydro-1H-cyclopenta[B]pyridine-3-carbonitrile (10.08%); phytol (2.01%); and vitamin E (3.18%). A significant reduction in free radical against DPPH was observed, which revealed antioxidant potential of MEAV. Conclusion Methanolic extract of Adhatoda vasica L. leaves consist of both polar and nonpolar components. GC-MS analysis was used to identify these compounds. The current work validates that the antioxidant activity of methanolic extract attributed to the presence of compounds like vitamin E, alkaloid, and terpene.

scholarly journals PRELIMINARY MYCOCHEMICAL, GAS CHROMATOGRAPHY–MASS SPECTROSCOPY ANALYSIS, AND ANTIMICROBIAL PROPERTIES OF CALOCERA VISCOSA (PERS.) FR.

2019 ◽  
pp. 86-93
Author(s):  
NAVEEN KUMAR NAIK S ◽  
ASHWATHANARAYANA R ◽  
RAJA NAIKA
Keyword(s):  
Gas Chromatography ◽  
Petroleum Ether ◽  
Mass Spectroscopy ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Ms Analysis ◽  
Medicinal Properties ◽  
Gas Chromatography Mass Spectroscopy

Objectives: Calocera viscosa, commonly called as the yellow stagshorn, is a jelly fungus, belongs to the family of Dacrymycetales, unknown for its medicinal properties and biological activities. Method: The sporocarps of C. viscosa (Pers.) Fr. were collected from Agumbe, Karnataka. Mycochemical and Gas chromatography–mass spectroscopy (GC–MS) analysis done by standard procedures and antibacterial activity was done by agar well diffusion method. Results: Physicochemical was analyzed and results revealed the highest percentage of alcohol-soluble extractives were present followed by ash content. Alcohol-soluble extractives were 20.76%, total moisture content (10.9%), and foreign matter (0.5%). Extraction was done by Soxhlet apparatus using petroleum ether, chloroform, and ethanol and subjected to qualitative mycochemicals analysis both petroleum ether and chloroform extract confirms less mycochemicals, whereas ethanolic extract revealed the presence of alkaloids, tannins, flavonoids, sterols, glycosides, terpenoids, and phenols. GC–MS analysis of ethanoic extract showed many known bioactive compounds in that, 19 compounds were unknown and 21 compounds were known for its medicinal properties, most of them were food additives and flavoring agents. Antibacterial potentials were studied against pathogenic bacteria revealed that ethanolic extract showed appreciable zone of inhibition against pathogenic bacteria, in that maximum zone of inhibition showed against Klebsiella pneumonia followed by Escherichia coli and Staphylococcus aureus. Conclusion: C. viscosa (Pers.) Fr. sporocarp can be explored for potential antibacterial with rich full of useful mycochemicals.

scholarly journals Phytochemical and Biological Investigation of Curcuma amada Leaves

2020 ◽  
Vol 19 (1) ◽  
pp. 9-13
Author(s):  
Md Sakhawat Hossain ◽  
Md Mostafizur Rahaman ◽  
Md Atikur Rahman ◽  
Jalal Uddin ◽  
Firoj Ahmed
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Clot Lysis ◽  
Zone Of Inhibition ◽  
Biological Investigation ◽  
Curcuma Amada ◽  
Antibacterial Screening ◽  
Ic50 Value ◽  
Vibrio Mimicus

Methanolic extract of Curcuma amada leaves was investigated for isolation of secondary metabolites by successive chromatographic separation (VLC, column chromatography and TLC) which yielded several purified compounds. Among them the structures of β-sitosterol and stigmasterol were determined by analysis of high resolution 1H NMR spectroscopic data and co-TLC with authentic samples. The crude methanolic extract of C. amada leaves and its different fractionates i.e. petroleum ether (PESF), chloroform (CSF) and aqueous (AQSF) soluble fractions were evaluated for antibacterial, antioxidant, cytotoxic and thrombolytic activities. In antibacterial screening, the CSF exhibited the highest inhibition against bacterial growth having zone of inhibition 12 mm compared to the standard kanamycin where the zone of inhibition was observed at 18 mm against gram negative Vibrio mimicus. Significant free radical scavenging activity was also exhibited by CSF with the IC50 value of 103.09 μg/ml as compared to tert-butyl-1-hydroxytoluene (BHT) having IC50 31.88 μg/ml. In cytotoxicity study, the crude extract showed significant lethality towards brine shrimp having with the LC50 value of 6.540 μg/ml as compared to the standard vincristine sulfate (0.451μg/ml). In the study for thrombolytic property, different extracts of C. amada exhibited clot lysis ranging from 17.24 to 43.55% as compared to standard streptokinase (93.75 %). Dhaka Univ. J. Pharm. Sci. 19(1): 9-13, 2020 (June)

scholarly journals Isolation and Characterization of Secondary Metabilites and Evaluation of Antimicrobial, Antioxidant and Thrombolytic Potentials of Erythrina variegata L. Bark

2019 ◽  
Vol 31 (8) ◽  
pp. 1842-1846
Author(s):  
Fatema Tabassum ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Mehedi Masud ◽  
Md. Imran Nur Manik ◽  
Monira Ahsan
Keyword(s):  
Methanolic Extract ◽  
Authentic Sample ◽  
Stem Bark ◽  
Moderate Activity ◽  
Zone Of Inhibition ◽  
Nmr Studies ◽  
Erythrina Variegata ◽  
Isolation And Characterization ◽  
Ic50 Value

Six compounds, sitosta-4-en-3-one (1), 3β,28-dihydroxyolean-12-en (2), scandenone (3), alpinum isoflavone (4), stigmasterol (5) and lupeol (6) were isolated from the methanol soluble extract of the stem bark of Erythrina variegata. The structure of the compounds was established by extensive NMR studies as well as co-TLC with authentic sample. The petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions of the methanolic extract of Erythrina variegata were evaluated for antimicrobial, antioxidant and thrombolytic properties. In the antimicrobial study, most of the fractions of the extract exhibited mild to moderate antimicrobial activity where the zone of inhibition were ranging from 7.69 to 19.51 mm. The chloroform soluble fraction showed significant antioxidant activity with IC50 value 66.28 μg/mL as compared to standard BHT (IC50 value 23.09 μg/mL). The thrombolytic property of different extracts of E. variegata exhibited moderate activity ranging from 31.25 to 57.78 %.

scholarly journals Hair Washing Formulations from Aloe elegans Todaro Gel: The Potential for Making Hair Shampoo

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Desta Berhe Sbhatu ◽  
Goitom Gebreyohannes Berhe ◽  
Abadi Gebreyesus Hndeya ◽  
Asmael Abdu ◽  
Afework Mulugeta ◽  
...  
Keyword(s):  
Foam Stability ◽  
Chemical Constituents ◽  
Coconut Oil ◽  
Capric Acid ◽  
Lemon Juice ◽  
Solid Content ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Physicochemical Methods ◽  
Gas Chromatography Mass Spectroscopy

This study aimed to describe the gross phytochemical constituents of Aloe elegans Todaro gel and evaluate the characteristics and quality of lab-made hair washing formulations prepared from the gel to show its potential in formulating hair washing shampoos. A. elegans gel mass was prepared from mature, healthy leaves collected from natural stand. Samples of 100% methanol extract of the gel were subjected to standard phytochemical screening and gas chromatography-mass spectroscopy (GC-MS) analysis. Five hair washing formulations (Fs) were, likewise, prepared by mixing 4.0–10.0 mL of gel with one (0.05 mL) to two (0.10 mL) drops of six synthetic and natural ingredients, namely, coconut oil, jojoba oil, olive oil, pure glycerin oil, lemon juice, and vitamin E. The gel to the total ingredient ratios (v/v) of the five formulations were 93 : 7 (F1), 94.5 : 5.5 (F2), 96.4 : 3.6 (F3), and 96.6 : 3.4 (F4 and F5). The formulations were evaluated using sensory inspection and common physicochemical methods. The phytochemical screening and GC-MS analysis revealed that A. elegans gel is the source of important chemical constituents used in the formulation of shampoos and similar products including saponins, capric acid, lauric acid, myristic acid, palmitic acid, linoleic acid, stearic acid, and phytol. Lab-made A. elegans hair washing formulations, especially those with 96.4–96.6% gel, were found to have similar characteristics and qualities with a common marketed shampoo. All the formulations were turbid with characteristic odor as the marketed shampoo. The pH values of the hair washing formulations (6.4–4.6) were comparable to those of the marketed shampoo (6.7). Formulations with higher proportion of gel had better foam stability, higher solid content (26–29%), higher surface tension (33–38 dynes/cm), shorter wetting time (150–160 sec), equivalent viscosities (26.45–26.73 poise), and conditioning performance than the marketed shampoo. These findings demonstrate that A. elegans gel mass can be used in the formulation of good-quality hair washing shampoos. We recommend future studies that aim to develop the phytochemical profile of the plant and a refined protocol of hair washing shampoo formulation.

scholarly journals In Vitro Antibacterial Activity of Spanish Moss (Tillandsia usneoides) Crude Extract Against Skin Infection in Wound Healing

2017 ◽  
Vol 9 (10) ◽  
Author(s):  
Faller E. M. ◽  
Kanes S. N. ◽  
Zajmi A. ◽  
Ramli M. D.
Keyword(s):  
Wound Healing ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Tillandsia Usneoides ◽  
Layer Chromatography

Wound healing is a dynamic phenomenon that results in the restoration of anatomic continuity and function on which can be delayed by pathogenic bacteria. This research was designed to explore the antimicrobial efficacy of Tillandsia usneoides against skin infections in wound healing. Physical and chemical evaluations were done through phytochemical screening and thin layer chromatography. The effect of methanolic, ethanolic and aqueous extracts of Tillandsia usneoides against pathogenic bacteria were evaluated on antimicrobial activities using disc diffusion and broth dilution susceptibility assay. The excision bioassay analysis was used in examining the wound healing process in mice. The result of the qualitative phytochemical screening showed the presence of flavonoids and alkaloids. Thin layer chromatography revealed a high Rf value for flavonoids (0.75mm) and alkaloids (0.60mm). The antibacterial assay showed a high zone of inhibition (ZI) for both methanolic extract (>23mm, >22mm and >20mm) and ethanolic extract (>22mm, >22mm and >17mm) for P.aeruginosa, S.aureus and S.epidermis respectively. Resistance against aqueous extract was observed based on the lowest zone of inhibition ( less than 4mm). Gentamicin was used as the positive control (>28mm) and DMSO as the negative control. The minimum inhibitory concentration and minimum bactericidal concentration results confirmed that methanolic and ethanolic extracts restrained the growth of tested bacteria in the range of 125 to 500mg/mL and showed bactericidal efficacy. Wound healing assay indicated that methanolic extract had a higher potency of wound closure (12 days; less than 1mm) compared to ethanolic and aqueous extracts (13days; >1.5mm and 13days: >1.8mm respectively). Povidone-Iodine was used as the gold standard (15days; less than 2mm) in the study. In conclusion, methanolic extract of T. usneoides has a great potential with regard to its antimicrobial and wound healing activity to be developed as a novel drug in the future.

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