scholarly journals HPTLC study to determine the antioxidant activity of dried leaves of Portulaca oleracea L.

2021 ◽  
Vol 12 (1) ◽  
pp. 254-261
Author(s):  
Kesri Nandan Sharma ◽  
Nitu Bhatnagar
Keyword(s):  
Free Radical ◽  
High Performance ◽  
Radical Scavenging ◽  
Portulaca Oleracea ◽  
Free Radical Scavengers ◽  
Stable Free Radical ◽  
Methanolic Extracts ◽  
Straightforward Method ◽  
Anti Oxidant ◽  
B Complex Vitamins

This present study involves the assessment of the anti-oxidant activity study of the sample which was obtained from the methanolic extracts of dried leaves of Portulaca Oleracea L.(common name Purslane). Purslane is a rich source of Vitamin A, Vitamin-C and some other B-complex vitamins like riboflavin, niacin, pyridoxine and carotenoids which are known powerful natural anti-oxidants.  Anti-oxidants are compounds that inhibit oxidation. This methanolic extract of leaves was evaluated for the determination of its anti-oxidant efficiency by using 1,1–diphenyl-2-picryl-hydrazyl (DPPH) by using Silica TLC plates on Camag High-Performance Thin Layer Chromatography (HPTLC) system using visionCATS software. Densitograms and chromatographs obtained show the presence of anti-oxidant activity. It is a rapid, inexpensive and straightforward method to measure anti-oxidant properties of substances after separation by HPTLC. It involves the use of the free radical, 2, 2-Diphenyl-1- picrylhydrazyl (DPPH) which is widely used to test the ability of compounds to act as free radical scavengers or hydrogen donors and to evaluate anti-oxidant activity. When Anti-oxidants substances react with DPPH, which is a stable free radical becomes paired off in the presence of a hydrogen donor (e.g., a free radical scavenging anti-oxidant) and is reduced to the DPPHH. As a consequence, the absorbance's decreased from the DPPH.   

scholarly journals AOP1, a New Live Cell Assay for the Direct and Quantitative Measure of Intracellular Antioxidant Effects

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 471 ◽  
Author(s):  
Camille Gironde ◽  
Mylène Rigal ◽  
Cécile Dufour ◽  
Christophe Furger
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Quantitative Measure ◽  
Cell System ◽  
Butylated Hydroxytoluene ◽  
Free Radical Scavengers ◽  
Live Cells ◽  
Antioxidant Compounds ◽  
Anti Oxidant

Taking advantage of Light Up Cell System (LUCS) technology, which allows for fine monitoring of reactive oxygen species (ROS) production inside live cells, a new assay called Anti Oxidant Power 1 (AOP1) was developed to specifically measure ROS and/or free-radical scavenging effects inside living cells. This method is quantitative and EC50s obtained from AOP1 dose-response experiments were determined in order to classify the intracellular antioxidant efficacy of 15 well known antioxidant compounds with different hydrophilic properties. Six of them (epigallocatechin gallate, quercetin, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethoxyquin, resveratrol) gave EC50s in the range of 7–64 μM, four (Trolox, catechin, epicatechin, EUK134) in the range of 0.14 to 1 mM, and 5 (sulforaphane, astaxanthin, α- and γ-tocopherols, vitamin E acetate) showed only partial or no effect. Interestingly, effects with measurable EC50s were observed for compounds with hydrophilic properties (LogP ≤ 5.3), while all antioxidants known to act at the plasma membrane level (LogP ≥ 10.3) had partial or no effect. Sulforaphane, a hydrophilic but strict Keap1/Nrf2 pathway enhancer, did not show any effect either. Importantly, AOP1 assay captures both antioxidant and prooxidant effects. Taken together, these results led us to the conclusion that AOP1 assay measures antioxidant effect of compounds that selectively enter the cell, and act as free radical scavengers in the cytosol and/or nucleus level.

scholarly journals Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits

2020 ◽  
Vol 8 (1) ◽  
pp. 29-35
Author(s):  
Messaoudi Mohammed ◽  
Merah Maroua
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Anthocyanin ◽  
Methanolic Extracts ◽  
Almost All

Since antiquity, phenolic compounds produced by plants were known as free radical scavengers and as powerful antioxidants. Huge interest has been made by researchers to the traditional uses of medicinal plants against illnesses related to oxidative stress. This study measures the correlation that can be existed between the antioxidant capacity and phytochemical s levels of four varieties of Ficus carica fruits, figs flowers or "Bakor" as called locally in Algeria. Therefore, extracts were assessed for determining their antioxidative potentials using both test of total antioxidant capacity and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test followed by quantitative phytochemical analysis to estimate the total flavonoid level (TFL), the total phenolic level (TPL), the total anthocyanin level (TAL) and the condensed tannins level of plants methanolic extracts. A positive correlation was observed between phenolics content and the antioxidant capacity of figs flowers methanol extracts. The methanolic extract of Bechar (MeOH Var.2) chelated 87,9± 1,23 % of the DPPH free radical with IC50 value equal to 0,185 mg/g DW. A high antioxidant ability of almost all extracts is, probably, related to the appreciable rates of flavonoids, phenolics and tannins showed by those fig extracts. The highest value of phenolics level was detected among the variety 1 methanolic extract of Bechar (MeOH Var.1) of 10,4 mg GAE/g DW.

scholarly journals Phytochemical Composition and Antioxidant Activity of Portulaca oleracea: Influence of the Steaming Cooking Process

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 94
Author(s):  
María del Pilar Fernández-Poyatos ◽  
Eulogio J. Llorent-Martínez ◽  
Antonio Ruiz-Medina
Keyword(s):  
Antioxidant Capacity ◽  
High Performance ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Portulaca Oleracea ◽  
Total Phenolic ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Cooking Process ◽  
Dried Extract

In this work, we compared the phenolic composition and antioxidant capacity of methanolic extracts of raw and steamed aerial parts of Portulaca oleracea L. Two new cyclo-dopa amides were identified, named oleraceins X and Y, along with six known ones (oleraceins A, B, C, N, J, and U). Compounds identification and quantification were done by high-performance liquid chromatography with diode array and mass spectrometry detections. The most abundant compounds were phenolic alkaloids (oleraceins), and the main quantified compounds were isocitric and citric acids, with concentrations of 500–550 and 440–600 mg/100 g dried extract, respectively. The study of both the influence of the steaming process in Portulaca oleracea L. and total phenolic content and radical scavenging assays (ABTS·+ and DPPH) were also carried out. The total individual phenolic content of raw Portulaca decreased from 1380 mg/100 g DE to 1140 mg/100 g DE after the steaming process. The antioxidant capacity in ABTS and DPPH assays decreased approximately 50 and 40%, respectively, after samples were cooked by steaming. The raw extracts presented the highest concentration of bioactive compounds, as well as higher antioxidant and radical scavenging values.

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

Antioxidant and Antifungal Properties of Benzimidazole Derivatives

2010 ◽  
Vol 65 (9-10) ◽  
pp. 537-542 ◽  
Author(s):  
Canan Kuş ◽  
Fatma Sözüdönmez ◽  
Benay Can-Eke ◽  
Tülay Çoban
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Radical Scavenging ◽  
Liver Microsomes ◽  
Thiobarbituric Acid ◽  
Benzimidazole Derivatives ◽  
Rat Liver Microsomes ◽  
Inhibitory Effects ◽  
Stable Free Radical ◽  
Radical Scavenging Properties

Antioxidant and radical scavenging properties of a series of 2-[4-(substituted piperazin-/ piperidin-1-ylcarbonyl)phenyl]-1H-benzimidazole derivatives were examined. Free radical scavenging properties of compounds 11-30 and 33 were evaluated for the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical. In addition the inhibitory effects on the NADPH-dependent lipid peroxidation levels were determined by measuring the formation of 2-thiobarbituric acid reactive substances (TBARS) using rat liver microsomes. Compound 33 which has a p-fluorobenzyl substitutent at position 1 exhibited the strongest inhibition (83%) of lipid peroxidation at a concentration of 10-3 M, while the nonsubstituted analogue 13 caused 57% inhibition. This result is fairly consistent with the antimicrobial activity results against both Staphylococcus aureus and Candida albicans.

scholarly journals Phenolic Profile and Antioxidant Evaluation of Mentha x Piperita L. (Peppermint) Extracts

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
H.J. Damien Dorman ◽  
Müberra Koşar ◽  
K Hüsnü C Başer ◽  
Raimo Hiltunen
Keyword(s):  
Free Radical ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Soluble Extract ◽  
Photodiode Array Detection ◽  
Mentha X Piperita ◽  
Soxhlet Apparatus

Seven extracts were prepared from Mentha x piperita (peppermint) leaves in sequence using a Soxhlet apparatus, viz. (40-60°) light petroleum (PE), dichloromethane (CH2Cl2), acetonitrile (ACN), ethyl acetate (EtOAc), methanol (MeOH), n-butanol and water (H2O) extracts. The phenolic and flavonoid content of each extract were estimated using spectrophotometric methods whilst a qualitative-quantitative analysis was made by reverse-phase high performance liquid chromatography coupled with photodiode array detection (HPLC-PDA). Each extract was assessed in a battery of six antioxidant-related assays so as to determine their iron(III) reductive, iron(II) chelating and free radical scavenging abilities. The MeOH-soluble extract contained the greatest content of total phenols and flavonoids based upon the Folin-Ciocalteu and 2-aminoethyl diphenylborate reagent data and HPLC-PDA analysis. Based upon the chromatographic and UV-spectral data, the leaves principally contained the cinnamic acid caffeic acid, the depside rosmarinic acid and flavonoids (flavones and flavanones). Eriocitrin (383.3 ± 2.2 mg/g extract) and rosmarinic acid (381.2 ± 1.9 mg/g extract) were the most abundant components identified within the leaves, whilst naringenin-7- O-glucoside (0.8 ± 0.01 mg/g extract) was the least abundant component identified being found only in the EtOAc-soluble extract. The EtOAc, ACN and H2O-soluble extracts demonstrated the most potent iron(III) reductive and 1,1′-diphenyl-2-picrylhydrayl, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) and hydroxyl free radical scavenging properties; however, the H2O and CH2Cl2-soluble extracts were the most potent extracts in the β-carotene-linoleic acid bleaching inhibition assay. In terms of iron(II) chelation – an important antioxidant property - the PE, MeOH and H2O extracts demonstrated moderate iron(II) chelating activity.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF TRIBULUS TERRESTRIS FRUIT AND MESUA FERREA FLOWER EXTRACTS: A COMPARATIVE STUDY

2018 ◽  
Vol 10 (3) ◽  
pp. 70
Author(s):  
Dakshayini P. N. ◽  
Mahaboob Basha P.
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Aluminium Chloride ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
P Value ◽  
Tribulus Terrestris ◽  
Ethanolic Extracts

Objective: This study aimed to investigate the antioxidants and free radicals scavenging properties of ethanolic extracts from Tribulus terrestris fruit (TTF) and Mesua ferrea flower (MFF).Methods: The antioxidant and free radical scavenging activities were measured by using standard protocols against phytoextracts used at 50, 100, 150, 200 and 250 µg/ml concentration. Total phenolic and flavonoid content were estimated using Folin-Ciocalteu and aluminium-chloride reagent assay methods.Results: Among the extracts, MFF showed the highest total antioxidant ability followed by TTF. With regard to various scavenging activities, MFF had the highest radical scavenging activity than TTF extract closely resembled the standards. Also, the reducing capacity on ferrous ion was high in MFF than TTF. The quantified phenolic contents of TTF and MFF ethanolic extracts were 27.0±0.36 and 57.0±0.32 mg gallic acid equivalent per gram extract respectively. A positive correlation (p value<0.001) was observed between phenolic content and free radical scavenging efficiencies.Conclusion: The results confirm that both MFF and TTF are important sources of natural antioxidants and serves as an effective free radical scavengers and/or inhibitors. Hence, both extracts are suitable plant-based pharmaceutical products can be used to handle 'free radical-mediated diseases.

scholarly journals Influence of phenolic acid content on the antioxidant capacity of hemicellulose from sorghum plant fractions

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 7933-7953
Author(s):  
Ryan J. Stoklosa ◽  
Renee J. Latona ◽  
Michael J. Powell ◽  
Madhav P. Yadav
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Process Economics ◽  
Scavenging Effect ◽  
Sorghum Bran ◽  
Sorghum Bagasse ◽  
Sorghum Plant ◽  
Methoxy Content

Recovered hemicellulose fractions from biorefineries have the potential to improve overall process economics during the production of biofuels or other high value chemicals. A common hemicellulose found in many agricultural feedstocks is arabinoxylan (AX). This work investigated the influence of ferulic and p-coumaric acids on the antioxidant capability of AX hemicellulose recovered from sorghum bran, biomass, and bagasse. Sorghum bagasse and sorghum biomass AX contained the largest quantities of bound ferulic and p-coumaric acids at 13.1 mg/100 g and 6.3 mg/100 g, respectively. Antioxidant performance showed that sorghum bagasse AX hemicellulose produced the best reducing capability, while sorghum biomass and sorghum bran AX hemicellulose performed better as free radical scavengers. A reduction in free radical scavenging, as determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, occurred for sorghum bagasse and sorghum biomass AX hemicellulose at higher polysaccharide concentrations, which was either caused by the solution properties of the AX hemicelluloses or DPPH reaction reversibility in the presence of phenolic compounds with methoxy content. Alternatively, H2O2 scavenging by the AX hemicellulose revealed a dose-dependent response. Although scavenging effect was reduced at higher concentrations, sorghum bagasse AX hemicellulose functioned as having the best antioxidant capacity with respect to total reducing capability.

scholarly journals Biological Activity of the Methanol and Water Extracts of the Fruits of Anthocyanin-Rich Plants Grown in South-West Poland

2015 ◽  
Vol 10 (3) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Paulina Strugala ◽  
Anna Dudra ◽  
Alicja Z. Kucharska ◽  
Anna Sokół-Łętowska ◽  
Dorota Wojnicz ◽  
...  
Keyword(s):  
Free Radical ◽  
Biological Effects ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Bacterial Biofilm ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Water Extracts ◽  
South West ◽  
Anti Inflammatory

In this study we focused on the chemical composition, antioxidant, anti-inflammatory and antibacterial properties of methanol and water extracts of fruits of six species grown in south-west Poland: blackberry, blackcurrant, chokeberry, cherry, gooseberry and raspberry. No general correlation could be drawn between extract chemical properties (i.e. antioxidant and free radical scavenging) and observed biological effects. However, blackcurrant and blackberry extracts (both methanol and water) appeared to be good antioxidants and free radical scavengers, as well as effective inhibitors of E. coli adhesion to the uroepithelial cells. On the other hand, the poorest antioxidants and free radical scavengers, cherry and gooseberry extracts, also poorly affected bacterial growth, swimming ability and adhesion to epithelial cells. Surprisingly, gooseberry extracts, which showed generally weak effects, appeared to be the most effective inhibitor of bacterial biofilm formation. Additionally, most of the studied extracts showed reasonably strong anti-inflammatory properties.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF HEMIDESMUS INDICUS (L.) R.BR. (ASCLEPIADACEAE).

2017 ◽  
Vol 10 (6) ◽  
pp. 200
Author(s):  
Abdul Kaffoor H ◽  
Muthuraj K ◽  
Arumugasamy K
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Stable Free Radical ◽  
Hemidesmus Indicus

Objective: A number of Indian medicinal plants have been used for thousands of years in a traditional system of medicine. Hemidesmus indicus is an important member of the Asclepiadaceae family. It is an endemic to the southern Western Ghats, India. The aim of the study was to investigate the free radical scavenging activity of H. indicus. Methods: The aqueous and methanol leaf extracts of H. indicus were assayed for radical scavenging activity, using the stable free radical 2,2-diphenyl- 1-picryl-hydrazyl-hydrate and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid). Results: The results revealed that the IC50 values of aqueous extract of H. indicus were found to be higher than that of the other solvent extracts. The free radical scavenging activity of the plant extracts may be due to the presence of phytoconstituents. Conclusion: In all the methods, the aqueous extract has exhibited the good scavenging activity and this arises that the plant has a potential antioxidant agent.

Sign in / Sign up

Export Citation Format

Share Document