Predicting the characteristics of copper oxide nanoparticles using Nyctanthes arbor-tristis flower extract via antibacterial study

Copper oxide (CuO) is an inorganic compound with monoclinic crystal structure. CuO nano particles attracted considerable attention due to its numerous applications in the field of optical, catalytic, mechanical, organic dye degeneration, biomedicine, pharmaceutics,cosmetics and different medical purposes. In the present study, copper oxide nanoparticles have been prepared by biological method using the flower extracts of Nyctanthes arbortristis as a reducing agent. The resulting samples were characterized using X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive analysis of X-rays (EDX), UV-Visible Spectroscopy (UV) and Antibacterial activity. From XRD studies, the average crystalline size of the obtained sample was calculated by Debye-Scherrer formula and it was found to be 33.13 nm. The band gap energy of the synthesized nano particle was estimated from UV studies and its value is 1.19 eV. The morphological characteristics were absorbed by SEM studies. The EDX and FTIR studies confirm the presence of Copper Oxide nano particles.The antibacterial activity of CuO-nano particles on selected bacteria was done using agar diffusion method.

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Promising Antibacterial Effect of Copper Oxide Nanoparticles against Several Multidrug Resistant Uropathogens

Pharmaceutical Sciences ◽

10.15171/ps.2018.31 ◽

2018 ◽

Vol 24 (3) ◽

pp. 213-218 ◽

Cited By ~ 2

Author(s):

Hadi Sedigh Ebrahim-Saraie ◽

Hamid Heidari ◽

Vahid Rezaei ◽

Seyed Mohammad Javad Mortazavi ◽

Mohammad Motamedifar

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Multidrug Resistant ◽

Copper Oxide Nanoparticles ◽

Diffusion Method ◽

Multiple Drug ◽

Oxide Nanoparticles ◽

Cuo Nps ◽

Conventional Antibiotics

Background: Recently, nanotechnology has been demonstrated to be a promising application to overcome the problem of antibiotic resistance. In the present study, we aimed to determine the antibacterial activity of copper oxide nanoparticles (CuO NPs) on several multiple-drug resistant (MDR) uropathogenic strains. Methods: This in vitro case-control study was performed on 4 uropathogenic bacteria including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. The antibacterial property was evaluated by well diffusion method at different concentrations of CuO NPs. Results: Overall, NPs concentration of 10, 25 and 50 µg/mL showed the remarkable antibacterial activity. A lower effect was seen against S. aureus strains. CuO NPs exhibited maximum bacterial growth inhibition against E. faecalis strains. In most of the cases, the zone of inhibition in 50 µg/mL concentration was closest to control positive antibiotics. Conclusion: In summary, CuO NPs as an alternative to conventional antibiotics that are currently used showed dose-dependent on antibacterial activity against different uropathogens, specificity towards pathogenic Gram-positive bacteria. This promising antibacterial activity of CuO NPs suggesting the development of NPs coatings on the different surface of biomedical materials for applications in different antimicrobial control systems.

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A Green Strategy to Synthesize and Characterization of CuO Nanoparticles for Antimicrobial Applications

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2021.36033 ◽

2021 ◽

Vol 9 (VI) ◽

pp. 4465-4468

Author(s):

S. D. Chavan

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Moringa Oleifera ◽

Antimicrobial Agents ◽

Copper Oxide Nanoparticles ◽

Advanced Technology ◽

Superior Performance ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Green Strategy

Green synthesis of nanoparticles is the recent and advanced technology in the fields of nanoscience and biotechnology which produces the eco-friendly and more efficient antimicrobial agents. Therefore, present study reported a simple, suitable and cost effective method of preparation of bioactive antimicrobial agents by the green strategy of copper oxide nanoparticles with Moringa oleifera through a green route method. In this green process the vital and varsatile medicinal quality plant i.e., Moringa oleifera leaves extract as a reducing agent as well as stabilizing agent were used to prepare the copper oxide nanoparticles. The synthesized copper oxide nanoparticles were characterized by UV- Vis, SEM, and TEM techniques. The antibacterial activity of the samples were tested by disc diffusion method against gram positive bacteria (S.aureus), gram negative bacteria (E.coli ). The size of synthesized copper oxide nanoparticles was in the ranges around 60-100 nm with dot and spherical shaped morphology. The antimicrobial activities of biofunctionalized copper oxide nanoparticles were observed significant inhibition activity. From this analysis, the green synthesized biofunctionalization method what we have suggested is exposed assure results in the antibacterial activity tests. Moreover, biofunctionalized copper oxide nanoparticles are shown superior performance in the opposed to of microbes.

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ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.10 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Haider Qassim Raheem ◽

Takwa S. Al-meamar ◽

Anas M. Almamoori

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Pseudomonas Fluorescens ◽

Wide Spectrum ◽

Copper Oxide Nanoparticles ◽

Morphological Properties ◽

Clinical Samples ◽

Oxide Nanoparticles ◽

Cuo Nps ◽

Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

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Green synthesis of copper oxide nanoparticles using Cedrus deodara aqueous extract for antibacterial activity

Materials Today Proceedings ◽

10.1016/j.matpr.2020.05.472 ◽

2020 ◽

Author(s):

M. Ramzan ◽

Raphael.M. Obodo ◽

S. Mukhtar ◽

S.Z. Ilyas ◽

Fahad Aziz ◽

...

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Green Synthesis ◽

Aqueous Extract ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Cedrus Deodara

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Green Synthesis, Characterization and Antibacterial Activity of Copper Oxide Nanoparticles Using Annona muricata Stem Extract

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2021.39498 ◽

2021 ◽

Vol 9 (12) ◽

pp. 1205-1214

Author(s):

Shivani Kushwaha

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Copper Sulphate ◽

Copper Oxide Nanoparticles ◽

Maximum Activity ◽

Cuo Nanoparticles ◽

Oxide Nanoparticles ◽

Gradual Change ◽

Annona Muricata ◽

Stem Extract

Abstract: Nanotechnology is a rising field of science and technology that deals with the particles having size in the range of 1 to 100 nm. Copper oxide nanoparticles has many properties like antifungal activity, antibacterial activity, optical properties, conductive properties, etc. Due to its demand of diversified use, copper oxide nanoparticles were fabricated using ecofriendly and non-toxic Annona muricata stem extract. The extract with copper sulphate pentahydrate showed gradual change in the colour of the extract from brown to green which indicates the CuO nanoparticles synthesis. The fabrication is followed by characterization of CuO nanoparticles using UV-vis spectroscope, FTIR, XRD and SEM. The characterization showed roughly spherical shaped nanoparticles in the range of 100nm with high crystalline monoclinic phase. FTIR absorption spectra conclude that the compounds attached with copper oxide nanoparticles could be polyphenols with aromatic ring. The CuO nanoparticles exhibited antibacterial activity; it showed the maximum activity against E.coli (18 mm). Keywords: Annona muricata, copper sulphate pentahydrate, FTIR, nanomaterials, SEM, XRD.

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