Optimization of temperature and drying time of indigenous cocktail yeast mold culture using response surface methodology (RSM)
The study was conducted to obtain an optimal combination of time and temperature of the drying process of indigenous cocktail yeast mold culture using RSM. The cocktail yeast mold culture was dried using an oven. The cocktail cultures contain Penicillium citrinum, Aspergillus niger, Acremonium strictum, and Candida famata, namely AC (Amylolytic Culture). The Response Surface Methods (RSM) with Design-Expert® 7.00 software, namely Mixture design with D-optimal was performed. The drying time was between 24- 48 hrs and the drying temperature was between 40-50oC. The total of 16 formulas of the combination of drying time and temperature for processing the dried cultures were produced by RSM. The response chosen was total viability of mold and yeast, water content, water activity, and pH. The result of optimization and verification was obtained by the model: pH (AC) = -0.058A - 1.56 x 10-003B + 7.13, where A = drying temperature ( oC), B = drying time (hr). The AC optimization was achieved at a combination of drying temperatures and time of 50oC for 48 hrs. Desirability values were 0.729. The optimum formula for AC has viability of total yeast mold of 7.39 x 106 CFU/g, moisture content of 5.62%, aw 0.303, and pH 4.18.