scholarly journals Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Nurul Cholifah ◽  
Ahmad Ridhay ◽  
Pasjan Satrimafitrah ◽  
Ruslan ◽  
Hardi Ys
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

scholarly journals Antibacterial Activity of Acacia nilotica Stem-Bark Fractions against Staphylococcus aureus and Escherichia coli

2019 ◽  
pp. 1-11
Author(s):  
R. D. Jabaka ◽  
S. B. Manga ◽  
Attah, D. Daniel ◽  
U. D. Nuhu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Acacia Nilotica ◽  
Diffusion Method ◽  
Bark Extract ◽  
Standard Drug

Aims: This research was carried out to determine the antibacterial activity of Acacia nilotica stem bark extract and bioactive fractions against the test bacteria (Staphylococcus aureus and Escherichia coli). Place and Duration of Study: Acacia nilotica was collected within Aliero town, Kebbi State, Nigeria between April and September, 2017. Methodology: The crude and bioactive fractions were obtained using soxhlet extraction and column chromatographic method respectively. The qualitative phytochemical screening was conducted to detect the presence of some phytochemical constituents in the crude extract and fractions. The antibacterial activity was determined at various concentrations (10, 50, 100, 150 and 200 mg/ml) using disc diffusion method. Results: The crude antibacterial activity indicated that ethanol extract showed higher activity than the n-hexane extract with 14.0±0.00 and 12.0±0.00 mm zones of inhibition compared with the control drug (10 µg Ciprofloxacin drug), which showed 14.0±0.00 and 13.0±0.00 mm zone of inhibition against the test bacteria. The MIC and MBC values determined for ethanol extracts against the test bacteria was 12.5 mg/ml and 25 mg/ml, while the MIC and MBC values obtained for n-hexane extracts were 25 and 50 mg/ml against the test bacteria. The bioactive fractions (Yellow, Purple and Blue Black Fractions) tested against the test bacteria showed higher activity compared with the crude extract. The phytochemical properties of the plant crude extract and the bioactive fractions indicated the presence of phenol, tannins, alkaloids, saponins, flavonoids, terpenoids, steroids and glycosides and  this attributed to the high antibacterial activities of 17.0±0.00 and 16.0±0.00 mm showed by the fractions against Staphylococcus aureus and 15.67± and 14.0±0.00 mm against Escherichia coli respectively. Conclusion: Acacia nilotica crude extract and fractions exhibited antibacterial activity which was comparable to the standard drug ciprofloxacin. This validates the folkloric medicinal use of this plant by the indigenous people of Aliero, Kebbi State.

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

Anti-Bacterial from Moringa oleifera Lamk Crude Extracts

2014 ◽  
Vol 1060 ◽  
pp. 223-226
Author(s):  
Perayot Pamonsinlapatham
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Moringa Oleifera ◽  
Soxhlet Extraction ◽  
Stem Bark ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Crude Extracts ◽  
Agar Diffusion Method

Moringa oleiferaLamk (Moringaceae) is a worldwide use for food and traditional medicine. In this study, plants were separated into seven parts: 1) Leaf, 2) Drum, 3) Branching, 4) Seed, 5) Outer stem, 6) Stem bark, and 7) Root, respectively. Then, they were extracted by two different methods: methanol soxhlet extraction and ethanol maceration. All crude extracts were tested for anti-bacterial activity by agar diffusion method and colorimetric micro-dilution plate technique. The results showed that 7 parts of Moringa crude extracts had low anti-bacterial activity againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawhen compared with standard antibiotic (ampicillin or gentamycin). In conclusion, anti-bacterial effects ofM. oleiferacrude extracts had low anti-bacterial activityin vitrotest.

scholarly journals ISOLATION OF SECONDARY METABOLITE COMPOUNDS AND ANTIBACTERIAL ACTIVITIES TESTS FROM HEXANE EXTRACT OF STEM BARK MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K

2018 ◽  
Vol 11 (8) ◽  
pp. 457 ◽  
Author(s):  
Usman Usman ◽  
Muh Amir M ◽  
Nunuk Hariani Soekamto ◽  
Ahyar Ahmad ◽  
Maulidiyah Maulidiyah
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Bark Extract ◽  
Separation And Purification ◽  
Inhibitory Zone

Objectives: This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellata (Houtt) Stapf var. degrabrata.Methods: M. umbellata stem bark was extracted by maceration using methanol solvent. Separation and purification were done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from the bark of M. umbellata was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Results: Phytochemical test showed that the hexane extracts of bark M. umbellata containing alkaloids and triterpenoids. Isolate D is a triterpenoid group compound, while FKa and FKb are steroid group compound. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12.0 mm. Isolate D has a weak inhibitory effect on all test bacteria. The highest diameters inhibition zone of isolated FKa compound against B. subtilis and S. aureus bacteria was 18.0 mm and 13.0 mm, respectively, whereas, the highest diameter inhibition of zone FKb compound against B. subtilis bacteria with inhibitory zone was 12.0 mm.Conclusion: The FKa compound from the bark of M. umbellata has the potential to be antibacterial because the compound is able to inhibit bacterial growth with ˃14 mm obstacle zone, especially against B. subtilis bacteria.

scholarly journals Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract on Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 3 (2) ◽  
pp. 26
Author(s):  
Asman Sadino ◽  
Idin Sahidin ◽  
Wahyuni Wahyuni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Weak Antibacterial Activity

The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique

scholarly journals Perbandingan Efektivitas Antibakteri antara Ekstrak Metanol Kulit Batang Kasturi dengan Ampisilin terhadap Staphylococcus aureusin Vitro

2016 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
M. Rizki Valian Akbar ◽  
Lia Yulia Budiarti ◽  
Edyson Edyson
Keyword(s):  
Staphylococcus Aureus ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Study Data ◽  
Diffusion Method ◽  
Bark Extract ◽  
Control Group ◽  
Tract Infections ◽  
Post Hoc

Abstract:Kasturi as a typical plant in South Kalimantan is one fruit that has many benefits. The barks of kasturi has proved to have benefits, especially to inhibit the activity of Staphylococcus aureus. Staphylococcus aureus is a gram positive bacteria that cause pneumonia, mastitis, and urinary tract infections. The bark of kasturi can be used in extract form. The objective of this research is to know the difference between the preparations of inhibition kasturi’s bark extract and ampicillin 30μg in inhibiting the growth of Staphylococcus aureus in vitro. This study used an experimental method consisting of 9 treatments with 3 repetitions. Treatment test in the methanol extract of the bark of kasturi 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100%. The control group used ampicillin and 70% methanol. Bacterial test was done by using a diffusion method. The parameter measured was the amount of inhibition zone (mm) which grown on media MH. Analysis of study data used One way Annova test and Post Hoc LSD test at α=0,05. The results showed that there were significant differences between the treatment kasturi’s bark extract 25%, 50%, 62.5%, 75%, 87.5% and 100% concentration different compared to ampicillin. Meanwhile, at the concentration of 37.5% (p <0.05) was not significant. The antibacterial effectiveness was obtained from the concentration of 100%. Key words: the bark of kasturi, methanol extract, Staphylococcus aureus, inhibition zone. Abstrak: Kasturi sebagai salah satu tanaman khas yang ada di Kalimantan Selatan merupakan salah satu buah yang memiliki banyak khasiat. Kulit batang kasturi terbukti memiliki manfaat terutama dapat menghambat aktivitas Staphylococcus aureus. Staphylococcus aureus merupakan bakteri gram positif yang menyebabkan penyakit pneumonia, mastitis, dan infeksi saluran kemih. Kulit batang kasturi dapat digunakan dalam bentuk ekstrak. Tujuan penelitian ini mengetahui perbedaan daya hambat antara sediaan ekstrak kulit batang kasturi dengan ampisilin 30µg dalam menghambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakan metode eksperimental yang terdiri dari 9 perlakuan dengan 3 kali pengulangan. Perlakuan yang di uji adalah ekstrak metanol kulit batang kasturi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100%. Dan kontrol perlakuan dengan ampisilin dan metanol 70%. Uji bakteri dilakukan menggunakan metode difusi. Parameter yang diukur adalah besaran zona hambat (mm) yang tumbuh pada media MH. Analisis data penelitian mengunakan uji One way ANNOVA dan uji Post Hoc LSD pada α=0,05. Hasil penelitian menunjukan bahwa terdapat perbedaan bermakna diantara perlakuan ekstrak kulit batang kasturi 25%, 50%, 62,5%, 75%, 87,5% dan 100% konsentrasi yang beda di bandingkan dengan ampisilin. Sedangkan pada konsentrasi 37,5% (p<0,05) tidak bermakna. Efektivitas antibakteri yang terbesar di peroleh dari konsentrasi 100%.Kata-kata kunci: kulit batang kasturi, ekstrak metanol, Staphylococcus aureus, zona hambat.

scholarly journals Pengujian Sifat Fisik dan Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Daun Lidah Mertua (Sansevieria trifasciata Prain)

2018 ◽  
Vol 1 (3) ◽  
pp. 078-085
Author(s):  
Setyo Nurwaini ◽  
Intan Dewi Saputri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Properties ◽  
Dosage Form ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Diffusion Method ◽  
Hand Sanitizer ◽  
Significant Difference

Daun Lidah mertua (Sansevieria trifasciata Prain) diketahui mengandung senyawa polifenol, flavonoid dan saponin yang merupakan agen antibakteri. Lidah mertua memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengetahui konsentrasi ekstrak daun lidah mertua dalam sediaan gel yang memiliki aktivitas antibakteri terbesar dalam penurunan jumlah angka kuman. Ekstraksi daun lidah mertua menggunakan metode maserasi dengan pelarut etanol 96%. Variasi konsentrasi ekstrak daun lidah mertua dalam sediaan gel hand sanitizer yang digunakan yaitu F1 (10%), F2 (15%) dan F3 (20%). Na CMC digunakan sebagai gelling agent dalam formulasi. Sediaan gel hand sanitizer daun lidah mertua diuji aktivitas antibakteri terhadap Escherichia coli menggunakan metode difusi sumuran, sifat fisik (organoleptik, homogenitas, pH, viskositas, daya sebar dan daya lekat) serta penurunan jumlah angka kuman dengan metode swabbing. Hasil uji aktivitas antibakteri gel hand sanitizer ekstrak daun lidah mertua memiliki zona hambat bakteri terhadap Escherichia coli pada F1, F2 dan F3 berturut-turut sebesar 7,33 mm, 8,67 mm dan 9,75 mm. Hasil evaluasi sifat fisik gel daun lidah mertua memenuhi kriteria gel yang baik, namun memiliki daya lekat kurang 1 detik. Hasil penurunan jumlah angka kuman gel hand sanitizer daun lidah mertua paling besar pada F3 sebesar 52%. Namun, analisa statistik dengan Anova One Way menunjukkan bahwa F1 (32%), F2 (35%) dan F3 (52%) tidak menunjukkan perbedaan yang signifikan dalam penurunan jumlah angka kuman. Berdasarkan hasil penelitian ini dapat disimpulkan gel hand sanitizer ekstrak daun lidah mertua memiliki kemampuan dalam menurunkan angka kuman dan dapat digunakan sebagai alternatif sebagai sediaan gel hand sanitizer.     Lidah Mertua leaves (Sansevieria trifasciata Prain) are known to contain polyphenols, flavonoids, and saponins which have antibacterial activity. Lidah Mertua leaves have antibacterial activity against Staphylococcus aureus and Escherichia coli. This study aimed to determine the concentration of Lidah Mertua leaves extract in a gel dosage form which showed the highest antibacterial activity in decreasing germ numbers. Lidah Mertua leaves extract was prepared using the maceration method with ethanol 96%. There was a various concentration of Lidah Mertua leaves extract in gel hand sanitizer, these include F1 (10%), F2 (15%) and F3 (20%). Na CMC is used as a gelling agent in the formulation. The evaluation of the antibacterial activity of hand sanitizer gel leaves was performed against Escherichia coli using disc diffusion method, meanwhile physical properties (organoleptic, homogeneity, pH, viscosity, dispersion and adhesion) and a decrease in the germ numbers were determined by swabbing method. The results of the antibacterial evaluation of gel hand sanitizer activity showed that lidah Mertua leaves extract had a bacterial inhibition zone against Escherichia coli in F1, F2 and F3 of 7.33 mm, 8.67 mm and 9.75 mm respectively. The results of the evaluation of the physical properties of the lidah mertua leaves extract showed that the gel met criteria of a good gel, but had adhesion less than 1 second. The highest activity in decreasing term number was shown in gel hand sanitizer of lidah mertua leaves extract at F3 which was 52%. However, statistical analysis using Anova One Way showed that F1 (32%), F2 (35%) and F3 (52%) did not show a significant difference in decreasing germ numbers. Based on the results of this study, it can be concluded that the  hand sanitizer gel from the extract of lidah mertua leaves has the ability to reduce the number of germs and can be used as an alternative as a gel hand sanitizer dosage form

scholarly journals Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma spinosum Segar dari Perairan Pulau Nain Sulawesi Utara

2021 ◽  
Vol 21 (1) ◽  
pp. 91
Author(s):  
Lena Jeane Damongilala ◽  
Fitje Losung ◽  
Verly Dotulong
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Disc Diffusion ◽  
Butanol Fraction ◽  
Inhibition Zones

Penelitian terhadap ekstrak metanol, fraksi etil asetat, dan fraksi butanol dari rumput laut Eucheuma spinosum menggunakan metode Disc Diffusion Test dilakukan untuk mengetahui peranannya sebagai antibakteri Escherichia coli ATCC 25922, dan Staphylococcus aureus ATCC 6538. Sebagai kontrol digunakan ekstrak (negatif) pelarut metanol, dan Amoxicilin. Konsentrasi sampel uji ditetapkan 10.000 ppm. Hasil pengujian menunjukkan adanya  aktivitas antibakteri yang ditandai dengan terbentuknya zona hambat terhadap bakteri Escherichia coli ATCC 25922.  Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi  butanol, dan fraksi etil asetat, ialah : 6,98 mm, 7,85 mm, dan 7,88 mm.  Nilai zona hambat tersebut, relatif sama untuk setiap sampel uji. Sampel uji juga menunjukkan terdapat aktivitas antibakteri, ditandai dengan terbentuknya zona hambat terhadap bakteri Staphylococcus aureus ATCC 6538.  Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi  butanol, fraksi etil asetat,  dan kontrol negatif pelarut metanol, ialah : 7,42 mm, 14.40 mm, 7,90 mm, dan 6,75 mm.  Zona hambat fraksi butanol lebih tinggi dibanding yang lain, ini menunjukkan bahwa aktivitas antibakteri Staphylococcus aureus pada fraksi butanol lebih tinggi dibanding fraksi lainnya.Kata kunci:  Antibakteri;  Eucheuma spinosum;  Escherichia coli; Staphylococcus aureus Antibacteria Activities of Extract Sea Algae  Eucheuma spinosum Fresh from Nain Island Waters North Sulawesi  ABSTRACTA research on antibacterial activity of methanol extract, ethyl acetate, and butanol fraction from Eucheuma spinosum seaweed using the disc diffusion method to know the role as  antibacterial activity was tested against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. Methanol was used as negative control while amoxicillin was used as positive control. The concentration of the test sample was set at 10,000 ppm. The antibacterial activity was indicated by the formation of an inhibition zone against the tested microorganisms. The inhibition zones against E.coli for methanol extract, butanol fraction, and ethyl acetate fraction were 6.98 mm, 7.85 mm, and 7.88 mm, respectively.  The value of the zone of inhibition is relatively the same for each sample.  In addition, the inhibition zones of methanol extract, butanol and ethyl acetate fraction against Staphylococcus aureus were 7.42 mm, 14.40 mm, 7.90 mm, and 6.75 mm, respectively. The inhibition zone of the butanol fraction is higher than the others, this indicated that the antibacterial activity for butanol fraction is higher than other fractions. Keywords: Antibacterial activity; Eucheuma spinosum; Escherichia coli; Staphylococcus aureus

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