Myrciaria Glazioviana Leaves Anti-Inflammatory Antinociceptive And Hepatoprotection Potential and Chemical Profile by Mass Spectrometry

In order to investigate the chemical profile of 14 specimens of Aplysina spp. marine sponges, we have developed a method based on LC-PDA-MS for the detection of bromotyrosine-derived metabolites. The method enabled the dereplication of three distinct chemotypes of bromotyrosine-derived compounds based on UV absorptions, which were further refined by electrospray ionization-mass spectrometry analysis of the brominated quasi-molecular ion clusters. This procedure led to either a single compound assignment, or a maximum of two possible isobaric compounds. The dereplication study indicated that the chemical profile of the 14 specimens of Aplysina spp. analyzed presented practically the same dibromotyrosine-derived compounds. The results obtained suggested a possible biogenetic pathway for the formation of dibromotyrosine-derived compounds of wide occurrence in Verongida sponges.

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Analysis of Solanum Americanum Mill. by Ultrafast Liquid Chromatography with Diode Array and Time-Of-flight Mass Spectrometry Detection with Evaluation of Anti-Inflammatory Properties in Rodent Models

Analytical Letters ◽

10.1080/00032719.2017.1399413 ◽

2018 ◽

Vol 51 (13) ◽

pp. 1973-1985 ◽

Cited By ~ 1

Author(s):

Julianna M. Vagula ◽

Bruno A. Rocha ◽

Alexandre R. Silva ◽

Narendra Narain ◽

Ciomar A. Bersani-Amado ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Time Of Flight ◽

Diode Array ◽

Rodent Models ◽

Mass Spectrometry Detection ◽

Flight Mass Spectrometry ◽

Solanum Americanum ◽

Anti Inflammatory

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Analysis of Cannabidiol, Δ9-Tetrahydrocannabinol, and Their Acids in CBD Oil/Hemp Oil Products

Medical Cannabis and Cannabinoids ◽

10.1159/000509550 ◽

2020 ◽

Vol 3 (1) ◽

pp. 1-13

Author(s):

Mahmoud A. ElSohly ◽

Timothy P. Murphy ◽

Ikhlas Khan ◽

Larry W. Walker ◽

Waseem Gul

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Natural Products ◽

Plant Material ◽

Oil Products ◽

Complete Picture ◽

Chemical Profile ◽

Cannabidiolic Acid ◽

Hemp Oil

Hemp products are readily available and are aggressively marketed for their health and medicinal benefits. Most consumers of these products are interested because of cannabidiol (CBD), which has taken the natural products industry by storm. The CBD and Δ9-tetrahydrocannabinol (Δ9-THC) concentrations in these products are often absent, and even where labeled, the accuracy of the label amounts is often questionable. In order to gain a better understanding of the CBD content, fifty hemp products were analyzed by gas chromatography coupled with mass spectrometry (GC-MS) for CBD, Δ9-THC, tetrahydrocannabinolic acid (Δ9-THCAA), and cannabidiolic acid (CBDA). Δ9-THCAA and CBDA are the natural precursors of Δ9-THC and CBD in the plant material. Decarboxylation to Δ9-THC and CBD is essential to get the total benefit of the neutral cannabinoids. Therefore, analysis for the neutral and acid cannabinoids is important to get a complete picture of the chemical profile of the products. The GC-MS method used for the analysis of these products was developed and validated. A 10-m × 0.18-mm DB-1 (0.4 μ film) column was used for the analysis. The majority of the hemp products were oils, one of the products was hemp butter, one was a concentrated hemp powder capsule, and another was a hemp extract capsule. Most of the products contained less than 0.1% CBD and less than 0.01% Δ9-THC. Three products contained 0.1–1% CBD, and 2 products contained 0.1–0.9% Δ9-THC. All of the samples appeared to be decarboxylated since the CBDA and Δ9-THCAA results were less than 0.001%. The developed method is simple, sensitive, and reproducible for the detection of Δ9-THC, Δ9-THCAA, CBD, and CBDA in CBD oil/hemp products.

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Quantification and validation of nine nonsteroidal anti‐inflammatory drugs in equine urine using gas chromatography–mass spectrometry for doping control

Drug Testing and Analysis ◽

10.1002/dta.2864 ◽

2020 ◽

Vol 12 (8) ◽

pp. 1065-1077

Author(s):

Eylem Funda Göktaş ◽

Erol Kabil ◽

Filiz Arıöz

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Doping Control ◽

Gas Chromatography Mass Spectrometry ◽

Chromatography Mass Spectrometry ◽

Inflammatory Drugs ◽

Anti Inflammatory

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Anti-Inflammatory Cembrane-Type Diterpenoids and Prostaglandins from Soft Coral Lobophytum sarcophytoides

Marine Drugs ◽

10.3390/md17080481 ◽

2019 ◽

Vol 17 (8) ◽

pp. 481 ◽

Cited By ~ 2

Author(s):

Hongjie Shen ◽

Xiaowan Liu ◽

Minghua Jiang ◽

Guangyuan Luo ◽

Zhenger Wu ◽

...

Keyword(s):

Nitric Oxide ◽

Mass Spectrometry ◽

Soft Coral ◽

High Resolution Mass Spectrometry ◽

Positive Control ◽

Ic50 Values ◽

Anti Inflammatory ◽

Modified Mosher’S Method ◽

Resolution Mass ◽

Better Than

Two new cembrane-type diterpenoids, lobophytins A (1) and B (3), and four new prostaglandins, (5E)-PGB2 (10), (5E)-13,14-dihydro-PGB2 (11), 13,14-dihydro-PGB2 (12) and 13,14-dihydro-PGB2-Me (13), together with ten known compounds were isolated from the soft coral Lobophytum sarcophytoides. The structures of these new secondary metabolites were identified by high resolution mass spectrometry (HR-ESIMS), nuclear magnetic resonance (NMR) and electron circular dichroism (ECD) analyses, as well as the modified Mosher’s method. Compounds 6, 7, 9, 10, 12, 13, 15 and 16 showed potential anti-inflammatory activity by inhibiting the production of nitric oxide (NO) in RAW264.7 cells that were activated by lipopolysaccharide, with IC50 values ranging from 7.1 to 32.1 μM and were better than the positive control indomethacin, IC50 = 39.8 μM.

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Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/2675287 ◽

2019 ◽

Vol 2019 ◽

pp. 1-19 ◽

Cited By ~ 3

Author(s):

Peng Huang ◽

Hongwen Ke ◽

Yang Qiu ◽

Mingchen Cai ◽

Jialin Qu ◽

...

Keyword(s):

Complex System ◽

Mass Spectrometry ◽

Mapk Signaling ◽

Network Pharmacology ◽

Disease Genes ◽

Chemical Profile ◽

Spectrometry Method ◽

Flight Mass Spectrometry ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

Qingre Lidan Decoction (QRLDD), a classic precompounded prescription, is widely used as an effective treatment for cholelithiasis clinically. However, its chemical profile and mechanism have not been characterized and elucidated. In the present study, a rapid, sensitive, and reliable ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established for comprehensively identifying the major constituents in QRLDD. Furthermore, a network pharmacology strategy based on the chemical profile was applied to clarify the synergetic mechanism. A total of 72 compounds containing flavonoids, terpenes, phenolic acid, anthraquinones, phenethylalchohol glycosides, and other miscellaneous compounds were identified, respectively. 410 disease genes, 432 compound targets, and 71 related pathways based on cholelithiasis-related and compound-related targets databases as well as related pathways predicted by the Kyoto Encyclopedia of Genes and Genomes database were achieved. Among these pathways and genes, pathway in cancer and MAPK signaling pathway may play an important role in the development of cholelithiasis. EGFR may be a crucial target in the conversion of gallstones to gallbladder carcinoma. Regulation of PRKCB/RAF1/MAP2K1/MAPK1 is associated with cell proliferation and differentiation. Thus, the fingerprint coupled with network pharmacology analysis could contribute to simplifying the complex system and providing directions for further research of QRLDD.

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Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro

Journal of Chromatographic Science ◽

10.1093/chromsci/bmz097 ◽

2020 ◽

Vol 58 (3) ◽

pp. 241-250

Author(s):

Sa Guo ◽

Mingzhen He ◽

Mi Liu ◽

Wengping Huang ◽

Hui Ouyang ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

High Performance ◽

Time Of Flight ◽

Tandem Mass ◽

Ether Extract ◽

Embelia Ribes ◽

Anti Inflammatory

Abstract Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases. Nevertheless, detailed information regarding its chemical composition is unavailable. Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes. A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified. Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract). Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL). Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract. These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.

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