scholarly journals Identifikasi dan Hitung Koloni Bakteri pada Tangan Perawat Kamar Operasi Instalasi Bedah Sentral (IBS) RSUD Arifin Achmad Pekanbaru Sebelum dan Sesudah Cuci Tangan dengan Antiseptik Klorheksidin Glukonat

2017 ◽  
Vol 5 (2) ◽  
pp. 82
Author(s):  
Devi Devi ◽  
Fauzia Andrini ◽  
Kuswan Ambar Pamungkas
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Nosocomial Infection ◽  
Sampling Method ◽  
Bacterial Colonization ◽  
Hand Washing ◽  
Bacillus Sp ◽  
Coagulase Negative Staphylococci ◽  
Hand Wash ◽  
Before And After

Handwashing is an important thing to do and the most effective way in order to prevent nosocomial infection andinfection control. The most popular surgery’s handwashing is fuerbringer method. The purpose of this study was toknow the effectivity of hand washing from the nurses that work at central operation theather Arifin Achmad GeneralHospital Pekanbaru before and after washing hand with chlorhexidine gluconat antiseptic. This study method waslaboratoric descriptive with 22 samples which got from total sampling method. Bacterial was found from all sample.Bacterial which were found from the samples before hand washing were Coagulase negative staphylococci (CONS)(62.9%), Bacillus sp (20%), Metichillin resistant Staphylococcus aureus (MRSA) (11.4%) and Metichillin sensitiveStaphylococcus aureus (MSSA) (5.7%). Bacterial which found after doing hand wash were CONS (52.9%), Bacillussp (17.7%), Escherichia coli (8.8%), MRSA (11.8%), and MSSA (5.9%). The result of this bacterial colonizationarea showed the reduction of bacterial colonization after doing hand wash in 90.1% sample , which mostly reductionwas happen from II degree to I (36.4%).

A Comparison of Hand Washing Techniques To Remove Escherichia coli and Caliciviruses under Natural or Artificial Fingernails

2003 ◽  
Vol 66 (12) ◽  
pp. 2296-2301 ◽  
Author(s):  
CHIA-MIN LIN ◽  
FONE-MAO WU ◽  
HOI-KYUNG KIM ◽  
MICHAEL P. DOYLE ◽  
BARRY S. MICHAELS ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tap Water ◽  
Hand Washing ◽  
Microbial Populations ◽  
Liquid Soap ◽  
E Coli ◽  
Hand Sanitizer ◽  
Before And After ◽  
Escherichia Coli Jm109 ◽  
Viral Indicators

Compared with other parts of the hand, the area beneath fingernails harbors the most microorganisms and is most difficult to clean. Artificial fingernails, which are usually long and polished, reportedly harbor higher microbial populations than natural nails. Hence, the efficacy of different hand washing methods for removing microbes from natural and artificial fingernails was evaluated. Strains of nonpathogenic Escherichia coli JM109 and feline calicivirus (FCV) strain F9 were used as bacterial and viral indicators, respectively. Volunteers with artificial or natural nails were artificially contaminated with ground beef containing E. coli JM109 or artificial feces containing FCV. Volunteers washed their hands with tap water, regular liquid soap, antibacterial liquid soap, alcohol-based hand sanitizer gel, regular liquid soap followed by alcohol gel, or regular liquid soap plus a nailbrush. The greatest reduction of inoculated microbial populations was obtained by washing with liquid soap plus a nailbrush, and the least reduction was obtained by rubbing hands with alcohol gel. Lower but not significantly different (P > 0.05) reductions of E. coli and FCV counts were obtained from beneath artificial than from natural fingernails. However, significantly (P ≤ 0.05) higher E. coli and FCV counts were recovered from hands with artificial nails than from natural nails before and after hand washing. In addition, microbial cell numbers were correlated with fingernail length, with greater numbers beneath fingernails with longer nails. These results indicate that best practices for fingernail sanitation of food handlers are to maintain short fingernails and scrub fingernails with soap and a nailbrush when washing hands.

scholarly journals ISOLASI BAKTERI DARI TANAH SEBAGAI PENGHASIL SENYAWA ANTIMIKROB

Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 46-51
Author(s):  
Sukmawati Sukmawati ◽  
FEBRIANTI ROSALINA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Sp ◽  
E Coli

Antibiotik adalah bahan obat yang sangat memegang peranan penting dalam menanggulangi penyakit infeksi. Senyawa antimikroba dapat diperoleh dari tumbuh-tumbuhan, dan mikroba. Senyawa antimikrob yang dihasilkan oleh mikroba memiliki keunggulan dibandingkan dengan antibiotik sintetik karena memiliki sifat yang lebih efektif, sebab targetnya spesifik serta toksisitasnya rendah. Tujuan dari penelitian yang telah dilakukan ialah untuk mengisolasi bakteri dari tanah yang mampu menghasilkan senyawa antimikrob, serta untuk menguji aktivitas penghambtannya terhadap  pertumbuhan Escherichia coli dan Staphylococcus aureus. Metode yang telah digunakan dalam penelitian ini ialah isolasi, purifikasi, dan seleksi bakteri dari sampel tanah dengan metode uji penghambatan sedangkan bakteri Bacillus sp. Merupakan bakteri pembanding. Berdasarkan hasil penelitian yang diperoleh terdapat 2 isolat yang berpotensi memiliki aktivitas antimikroba yaitu isolat 1 dan isolate 4. Isolat 1 lebih berpotensi menghambat E. coli dengan indeks hambat 4.0 mm dibandingkan dengan penghambatan S. aureus dengan indeks hambat 3.1 mm. Sedangkan isolat 4 lebih berpotensi menghambat S. aureus dengan indeks hambat 2.8 mm dibandingkan dengan penghambatan terhadap E. coli dengan indeks hambat 1.4 mm.

scholarly journals A mHealth intervention to promote hand-washing and cell phone cleaning in medical residents of a public hospital in Peru

2021 ◽  
Vol 15 (03) ◽  
pp. 428-435
Author(s):  
Maria Lazo-Porras ◽  
Matilde Corante ◽  
Tania De La Cruz-Saldaña ◽  
Ingrid Bohórquez ◽  
Kalina Campos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Hand Hygiene ◽  
Cell Phones ◽  
Bacterial Colonization ◽  
Hand Washing ◽  
Medical Residents ◽  
Chi Square ◽  
Mhealth Intervention ◽  
Hospital Acquired ◽  
The Impact

Introduction: We explore the limitations to adherence of hand-washing and evaluate the impact of a mHealth intervention for hand hygiene in residents. Methodology: We explore resident's perspectives about Hospital-acquired infections (HAI) and hand washing. In baseline, participants completed socio-demographic characteristics and hand-washing habits survey. The intervention consisted of sending SMS three times a week for two months about hand hygiene and “five moments” for hand washing. The cultures of hands and cell phones were analyzed at baseline, 2 months and 4 months. We used chi-square and adjusted Generalized Estimating Equations. Results: Five physicians were interviewed and 33 participants were included for quantitative analysis. Critical barriers that hinder hand washing were identified. The proportion of Staphylococcus aureus in hands was 54.5% at baseline and was significantly reduced at 2 months follow-up (p = 0.009), but, benefit was lost when the intervention was discontinued; Escherichia coli and Klebsiella sp. were observed in 22.2% of hands, no changes were noted with intervention. In cell phones, there was a tendency to lower values of bacterial colonization after intervention for Staphylococcus aureus growth. Conclusions: High prevalence of contamination in hands and phones in medical residents were found. Serious barriers to compliance with hand washing must be overcome. It is possible that prolonged or continuous interventions could be necessary to optimize hand washing and reduce hand and cell phones contamination.

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

Microbiological Effects of Hand Washing at a Beef Carcass–Breaking Facility†

2003 ◽  
Vol 66 (3) ◽  
pp. 493-496 ◽  
Author(s):  
C. O. GILL ◽  
J. C. McGINNIS
Keyword(s):  
Escherichia Coli ◽  
Hand Washing ◽  
E Coli ◽  
Before And After ◽  
Disinfectant Solution ◽  
The Mean ◽  
Beef Carcass

The hands of workers in the carcass-breaking facility at a beef packing plant were sampled by rinsing. Total aerobes, coliforms, and Escherichia coli were enumerated for each sample. The numbers of bacteria recovered from duplicate groups of 25 hand samples collected before and after hands were washed with an antibacterial gel, rinsed in a disinfectant solution, washed with the gel and rinsed with the disinfectant, or washed in the disinfectant for 20 s were similar for samples collected before work began after breaks. The numbers of bacteria recovered from samples collected before and after hands were washed with the antibacterial gel and rinsed in the disinfectant solution were similar for samples collected after work as well. However, the mean numbers of aerobes recovered from the four groups of hand samples after work were all >6.5 log CFU per hand, while 9 of the 10 corresponding values for groups of hand samples collected before work were <6.5 log CFU per hand; the total numbers of coliforms recovered from three groups of hand samples collected after work were >4 log CFU/25 hands, while 9 of the corresponding values for groups of hand samples collected before work were <4 log CFU/25 hands. The total numbers of E. coli recovered from all groups of hand samples collected after work were >3.5 log CFU/25 hands, while 9 of the corresponding values for groups of hand samples collected before work were <3 log CFU/25 hands. Thus, although washing and/or rinsing apparently did not reduce the numbers of bacteria on hands, fewer bacteria were recovered from hands before than after work.

Evaluation of an Automated Beef Carcass Washing and Sanitizing System under Production Conditions1,2

1987 ◽  
Vol 50 (7) ◽  
pp. 562-566 ◽  
Author(s):  
MAYNARD E. ANDERSON ◽  
HAROLD E. HUFF ◽  
HUGH D. NAUMANN ◽  
ROBERT T. MARSHALL ◽  
JOHN M. DAMARE ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Hand Washing ◽  
Aerobic Bacteria ◽  
Before And After ◽  
Beef Carcass

Beef half carcasses were hand- or machine-washed and then machine-sanitized with 1.5% acetic acid. Sanitizer was applied at 14.4 or 52°C. Counts of Escherichia coli, Enterobacteriaceae and aerobic bacteria, made on samples collected by excision of tissues before and after treatments, demonstrated that machine washing and sanitizing reduced counts more than did hand washing. Counts were reduced more by hot than cool acetic acid. Percentages of samples with counts of log10 5.0/200 cm2 or higher after treatment were 26 and 46 for samples from carcasses sanitized with 1.5% acetic acid at 52 and 14.4°C, respectively. After hand washings 65% of the samples had these high counts.

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2019 ◽  
Vol 10 (1) ◽  
pp. 50-55
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening diabetic infections are usually polymicrobial involving multiple aerobic and anaerobic organisms. Methodology: The present study was a cross sectional study, conducted in the department of surgery and microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September' 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is 'any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus'. Results: Majority 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillin-clavulanic acid, amikacin, piperacillin-tazobactam. In Coagulasenegative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem. Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Escherichia coli and Citrobacter spp. Anwer Khan Modern Medical College Journal Vol. 10, No. 1: Jan 2019, P 50-55

scholarly journals Anaerobic Spondylodiscitis due toFusobacteriumSpecies: A Case Report Review of the Literature

2015 ◽  
Vol 2015 ◽  
pp. 1-5
Author(s):  
Tiffany N. Latta ◽  
Aimee L. Mandapat ◽  
Joseph P. Myers
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Case Report ◽  
Epidural Abscess ◽  
Vertebral Osteomyelitis ◽  
Review Of The Literature ◽  
Coagulase Negative Staphylococci ◽  
Gram Negative

Spondylodiscitis caused byFusobacteriumspecies is rare. Most cases of spontaneous spondylodiscitis are caused byStaphylococcus aureusand most postoperative cases are caused byStaphylococcus aureusor coagulase-negative staphylococci.Escherichia coliis the most common Gram-negative organism causing spondylodiscitis.Fusobacteriumspecies are unusual causes for anaerobic spondylodiscitis. We report the case of a patient with spontaneous L2-L3 spondylodiscitis, vertebral osteomyelitis, and epidural abscess caused byFusobacteriumspecies and review the literature for patients withFusobacteriumspondylodiscitis.

Bacterial Colonization of the Skin Following Aseptic Preoperative Preparation and Impact of the Use of Plastic Adhesive Drapes

2012 ◽  
Vol 15 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Karin Falk-Brynhildsen ◽  
Örjan Friberg ◽  
Bo Söderquist ◽  
Ulrica G. Nilsson
Keyword(s):  
Sampling Method ◽  
Bacterial Colonization ◽  
Surgical Team ◽  
Preoperative Preparation ◽  
Coagulase Negative Staphylococci ◽  
Established Method ◽  
Wound Contamination ◽  
Skin Flora ◽  
The Impact ◽  
Chlorhexidine Solution

Surgical site contamination, for example, with coagulase-negative staphylococci, probably derives from both the patient’s own skin flora and those of the surgical team. Despite preoperative antiseptic preparation with chlorhexidine solution, complete sterilization of the skin is not possible and gradual recolonization will occur. Plastic adhesive drape is an established method used to prevent direct wound contamination from adjacent skin. In this study, the time to skin recolonization after antiseptic preparation was measured and the impact of using plastic adhesive drape on this recolonization was evaluated. Repeated bacterial sampling using three different methods over 6 hr was conducted after antiseptic preparation in 10 volunteers. Recolonization of skin was observed after 30 min with plastic drape and after 60 min without plastic drape; there were significantly more positive cultures with the plastic drape than without (31% vs. 7.5%, respectively, p < .001). Sampling with a rayon swab was the most sensitive sampling method. In conclusion, covering the skin with a plastic adhesive drape seems to hasten recolonization of the skin after antiseptic preparation. However, clinical trials to confirm this finding are warranted.

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