Spatial and temporal control of drug delivery. Preparation of novel drug carrier based on the self-association of block copolymer.

Objective: This study aimed to examine whether DC101 (anti-VEGFR2 antibody)- modified micelles have applications as novel drug delivery devices, which allow small molecule antiangiogenic agents to deliver to angiogenic sites on a murine laser-induced choroidal neovascularization (CNV) model. Materials and Method: CNV was induced by photocoagulation on the unilateral eye of each mouse under anesthesia. Immediately after laser coagulation, E7974-loaded DC101-modified micelles and motesanib-loaded DC101-modified micelles were intravitreally administrated. Two weeks after photocoagulation, CNV was visualized using fluorescein-conjugated dextran (MW=2,000 kDa), and the CNV area was measured in retinal pigment epithelium (RPE)-choroidal flat mounts. Results: Intravitreal administration of both DC101-modified micelles loaded with E7974 at 2 µM and motesanib at 2 µM significantly reduced CNV area in the murine laser-induced CNV model at a clearly lower concentration than the effective dose of each agent. Conclusion: These results suggest that DC101-modified micelle might be effective drug carrier system for treating CNV and other ocular angiogenic diseases.

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Synthesis of PMMA-b-PS Block Copolymer by Emulsion ATRP and its Self-Assembly Property

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.705.115 ◽

2013 ◽

Vol 705 ◽

pp. 115-119

Author(s):

Bao Yong Tian ◽

Er Jun Tang ◽

Miao Yuan ◽

Rui Xia Hao ◽

Cun Man Li ◽

...

Keyword(s):

Drug Delivery ◽

Block Copolymer ◽

Self Assembly ◽

Potential Application ◽

Morphological Characteristic ◽

Living Polymerization ◽

The Self ◽

Emulsion System ◽

Copolymer Concentration ◽

Ft Ir

The well-defined block copolymer PMMA-b-PS was prepared by two-step ATRP in emulsion system. GPC results indicate that Mn increased linearly with conversion and polydispersity remained relatively narrow. It presents the characteristics of living polymerization in emulsion system. FT-IR demonstrated that block copolymer PMMA-b-PS could be successfully synthesized by ATRP with macroinitiator PMMA-Cl in emulsion system. The morphological characteristic of the self-assembly depends on the block copolymer concentration and transforms between spheres and rodlike micelles. The property indicates a perfect potential application in drug delivery materials.

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Polymerization of dopamine accompanying its coupling to induce self-assembly of block copolymer and application in drug delivery

Polymer Chemistry ◽

10.1039/d0py00085j ◽

2020 ◽

Vol 11 (16) ◽

pp. 2811-2821 ◽

Cited By ~ 3

Author(s):

Yudian Qiu ◽

Zongyuan Zhu ◽

Yalei Miao ◽

Panke Zhang ◽

Xu Jia ◽

...

Keyword(s):

Drug Delivery ◽

Block Copolymer ◽

Self Assembly ◽

The Self ◽

Ordered Structures

The polymerization of dopamine and its coupling occur in succession, which synergistically induces the self-assembly of block copolymer to yield ordered structures, including micelles and vesicles.

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The Chemistry of Bioconjugation in Nanoparticles-Based Drug Delivery System

Advances in Condensed Matter Physics ◽

10.1155/2015/198175 ◽

2015 ◽

Vol 2015 ◽

pp. 1-27 ◽

Cited By ~ 29

Author(s):

Karolina Werengowska-Ciećwierz ◽

Marek Wiśniewski ◽

Artur P. Terzyk ◽

Sylwester Furmaniak

Keyword(s):

Drug Delivery ◽

Drug Delivery Systems ◽

Polymeric Nanoparticles ◽

Physical Adsorption ◽

Drug Carrier ◽

Anticancer Therapy ◽

Modern Drug ◽

Polycyclic Groups ◽

Novel Drug ◽

Small Elements

Nanomedicine is, generally, the application of nanotechnology to medicine. The term nanomedicine includes monitoring, construction of novel drug delivery systems, and any possible future applications of nanotechnology and nanovaccinology. In this review, the most important ligand-nanocarrier and drug-nanocarrier bioconjugations are described. The detailed characterizations of covalently formed bonds between targeted ligand and nanocarrier, including amide, thioether, disulfide, acetyl-hydrazone and polycyclic groups, are described. Also, the coupling of small elements and heteroatoms in the form of R-X-R the “click chemistry” groups is shown. Physical adsorption and chemical bonding of drug to nanocarrier surface involving drug on the internal or external surfaces of nanocarriers are described throughout possibility of the formation of the above-mentioned functionalities. Moreover, the most popular nanostructures (liposomes, micelles, polymeric nanoparticles, dendrimers, carbon nanotubes, and nanohorns) are characterized as nanocarriers. Building of modern drug carrier is a new method which could be effectively applied in targeted anticancer therapy.

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Optical Detection of Charged Biomolecules: Towards Novel Drug Delivery Systems

Acta Polytechnica ◽

10.14311/1450 ◽

2011 ◽

Vol 51 (5) ◽

Author(s):

V. Petráková

Keyword(s):

Drug Delivery ◽

Cell Imaging ◽

Drug Carrier ◽

Electrical Potential ◽

Diamond Surface ◽

Attractive Alternative ◽

Covalent Bonds ◽

Detection Tool ◽

Novel Drug ◽

Work Done

This paper presents work done on developing optically-traceable intracellular nanodiamond sensors, where the photoluminescence can be changed by a biomolecular attachment/delivery event. Their high biocompatibility, small size and stable luminescence from their color centers make nanodiamond (ND) particles an attractive alternative to molecular dyes for drug-delivery and cell-imaging applications. In our work, we study how surface modification of ND can change the color of ND luminescence (PL). This method can be used as a novel detection tool for remote monitoring of chemical processes in biological systems. Recently, we showed that PL can be driven by atomic functionalization, leading to a change in the color of ND luminescence from red (oxidized ND) to orange (hydrogenated ND). In this work, we show how PL of ND changes similarly when interacting with positively and negatively charged molecules. The effect is demonstrated on fluorinated ND, where the high dipole moment of the C-F bond is favorable for the formation of non-covalent bonds with charged molecules. We model this effect using electrical potential changes at the diamond surface. The final aim of the work is to develop a “smart” optically traceable drug carrier, where the delivery event is optically detectable.

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Formulation, Characterization and In vitro Drug Delivery of Vitexin Loaded Liposomes

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.2.2 ◽

2021 ◽

Vol 14 (2) ◽

pp. 5364-5371

Author(s):

Dr.S.Bhagavathy Sivathanu ◽

Shivapriya G ◽

Shivapriya G

Keyword(s):

Drug Delivery ◽

Drug Carrier ◽

Egg Yolk ◽

Drug Carriers ◽

Good Choice ◽

Target Site ◽

Carrier System ◽

Liposome Preparation ◽

Drug Carrier System ◽

Novel Drug

Liposome is a spherical vesicle which contains atleast one lipid bilayer. Liposomes are used as a novel drug carriers because of its hydrophobic and hydrophilic nature, it has many advantages in the field of medical sciences. There are some other drug carriers like dendrimers, micelles, niosomes. Out of all, liposomes are considered to be the most promising agent for drug delivery. The uniqueness of liposome is when it is used as a pharmaceutical drug, it acts as a natural receptor. Thus it acts as an antigen and binds with the antibody (cancer cell) without causing any damage to the adjacent cells. For the synthesis of liposomes, a phospholipid is required. The liposomes can be synthesized using egg yolk and chloroform. So the basic phospholipid is obtained from egg yolk. For more stability, the liposomes are prepared using popc. The present work discuss about the effective preparation of drug loaded liposomes using popc (1- palmitoyl-2-oleoyl-sn-glycero-3- phosphocholine). POPC is an important phospholipid for biophysical experiments. Additionally chloroform is used as the solvent for the liposome preparation. The drug chosen for liposome loading is vitexin (vxn), which is an effective therapeutic agent against inflammation and cancer. The vesicular size, shape, drug entrapment efficacy, stability, electrochemical property and drug releasing property of the formulated liposomes were characterized. The results showed that the formulated liposomes are considered as the better drug carrier system and good choice for biotransformation within the cell to reach the target site such as cancer cells. Even though available treatments like chemotherapy and radiation therapy, causes damage to the surrounding cells, the alternative drug transferring system such as liposomal mediated drug transfer within the cell is considered as good choice of treatment to avoid such complications. The aim of liposome mediated drug carrier system is to develop a method to reach the drug to the target site. After drug delivery at the target site, the liposomes are fused within the surface of the body. This is because of the pH of liposomes, which is at 7.4 and temperature is maintained at 37 oC. So, the vxn loaded liposomes are considered as the novel drug carriers for the successful targetted drug delivery.

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Tailoring Nano-copolymer/CNTs Composite and Its Application in Drug Delivery

10.21203/rs.3.rs-215288/v1 ◽

2021 ◽

Author(s):

M. K. Darwish ◽

M. S. Said ◽

A. A. El-Sayed ◽

R. A. Sobh ◽

A. A. Abdel Khalek

Keyword(s):

Drug Delivery ◽

Drug Carrier ◽

Entrapment Efficiency ◽

Drug Carriers ◽

Feed Composition ◽

Particle Size Analyzer ◽

Monomer Feed ◽

Novel Drug ◽

Mcf 7

Abstract This work aimed to overcome the main drawbacks of some essential anticancer drugs as 5-Fluorouracil (5-FU) by controlled loading with novel drug carriers. By a differential microemulsion technique, nanosized particles derived from a copolymer of poly(methyl methacrylate (MMA) and 2-hydroxyethyl methacrylate (HEMA)) with different monomer ratios have been synthesized and used as a drug carrier. Poly(MMA-co-HEMA)/MWCNT nanocomposite was also synthesized using an in-situ microemulsion polymerization technique and used as a 5-FU carrier. Different techniques have characterized these ground-breaking drug delivery systems such as FT-IR, XRD, TEM, TGA, zeta potential, and a particle size analyzer. The effects of monomer feed composition, 5-FU content, and MWCNTs content on morphological and structural properties, in-vitro 5-FU release, and entrapment efficiency (EE%) have been studied. It was noted that the inclusion of MWCNTs in the 5-FU-loaded polymer increases the thermal stability and raises the entrapment efficiency (EE%) to hit 99% at CNTs:5-FU ratio of 2:1. The anticancer drug release from the co-polymeric nanospheres depends on the HEMA ratio, 5-FU/copolymer ratio, CNT/5-FU ratio, and the pH of the medium. The optimized nanocomposite demonstrated higher anti-tumor activity against the cell lines CaCo-2, MCF-7, and HepG-2 and higher cytotoxicity against HepG-2 relative to CaCo-2 and MCF-7.

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