scholarly journals The effects of subchronic exposure to ketoprofen on early developmental stages of common carp

2013 ◽  
Vol 82 (3) ◽  
pp. 343-347 ◽  
Author(s):  
Eva Prášková ◽  
Stanislava Štěpánová ◽  
Lucie Chromcová ◽  
Lucie Plhalová ◽  
Eva Voslářová ◽  
...  
Keyword(s):  
Common Carp ◽  
Surface Waters ◽  
Developmental Stages ◽  
Experimental Conditions ◽  
Environmental Concentration ◽  
Negative Effect ◽  
Rate Evaluation ◽  
The Impact ◽  
Early Developmental

Ketoprofen residues have been found in surface water where they present a potential risk to nontarget aquatic species. The aim of this study was to assess the impact of ketoprofen in surface waters on fish under experimental conditions. Subchronic toxic effects on 300 embryos and larvae of common carp (Cyprinus carpio) were investigated during a 30 day toxicity test with concentrations of ketoprofen: 0.003, 2.1, 6.3 and 21 mg/l. The exposure to ketoprofen showed no effect on mortality, but we observed significant delay (P < 0.05) in hatching in day 3 in fish exposed to all concentrations of ketoprofen. Significant delays (P < 0.05) in development were revealed at 2.1, 6.3 and 21 mg/l ketoprofen. On the basis of weight and growth rate evaluation and the determination of developmental stages, the lowest observed effect concentration was 0.003 mg/l. According to these results, the reported environmental concentration of ketoprofen in Czech rivers could have a negative effect on the growth and development of carp embryos and larvae. These tests have not yet been performed. Further research and search for a mechanism to reduce the incidence of ketoprofen in the waters by better wastewater treatment is required.

scholarly journals The Effects of Subchronic Exposure to Terbuthylazine on Early Developmental Stages of Common Carp

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Stanislava Štěpánová ◽  
Lucie Plhalová ◽  
Petra Doleželová ◽  
Miroslav Prokeš ◽  
Petr Maršálek ◽  
...  
Keyword(s):  
Growth Rate ◽  
Common Carp ◽  
Developmental Stages ◽  
Growth Parameters ◽  
Total Body Length ◽  
Control Group ◽  
Experimental Conditions ◽  
Environmental Concentration ◽  
Effect Concentration ◽  
The Impact

The aim of this study was to assess the impact of terbuthylazine in surface waters on fish under experimental conditions. Subchronic toxic effects on embryos and larvae of common carp (Cyprinus carpio) were investigated during a 30-day toxicity test. The exposure to terbuthylazin showed no effect on mortality, but significant differences (P<0.0001) were revealed on weight and growth parameters at concentrations of 520 and 820 μg/L. The inhibition of specific growth rate at concentrations of 520 and 820 μg/L was 14% compared to the control group. No significant negative effects on total body length and body weight were observed at lower concentrations (0.9 and 160 μg/L). The concentrations 520 and 820 μg/L were associated with a delay in development compared to other experimental groups and controls. On the basis of weight and growth rate evaluation and determination of developmental stages, the No Observed Effect Concentration (NOEC) of terbuthylazine was estimated at 160 μg/L and the Lowest Observed Effect Concentration (LOEC) was 520 μg/L. According to these results, the reported environmental concentration of terbuthylazine in Czech rivers does not impact growth, development, morphology, or histology of carp embryos and larvae.

Evaluation of biodegradation kinetics of organic wastewater in a laboratory river model

1994 ◽  
Vol 30 (10) ◽  
pp. 229-235 ◽  
Author(s):  
J. Zagorc-Koncan ◽  
M. Dular
Keyword(s):  
Comparative Study ◽  
Surface Waters ◽  
Organic Pollution ◽  
Wastewater Effluent ◽  
Biodegradation Kinetics ◽  
River Waters ◽  
Point Of Entry ◽  
The Impact ◽  
Kinetics Of

A laboratory river model for the study of biodegradation kinetics of organic pollution in surface waters is presented. It enables an engineering - technological prediction of the impact of wastewater biodegradation on DO and TOC profile in a river downstream from the point of entry of wastewater effluent thus providing rapidly and inexpensively significant design information to an environmental scientist or engineer. The method was applied to the determination of degradation of pharmaceutical wastewaters. Biodegradation during adaptation and a comparative study on organic constituents, biodegradation rates in polluted and unpolluted river waters were simulated.

scholarly journals Interpretation of ponded infiltration data using numerical experiments

2016 ◽  
Vol 64 (3) ◽  
pp. 289-299 ◽  
Author(s):  
Michal Dohnal ◽  
Tomas Vogel ◽  
Jaromir Dusek ◽  
Jana Votrubova ◽  
Miroslav Tesar
Keyword(s):  
Hydraulic Conductivity ◽  
Saturated Hydraulic Conductivity ◽  
Experimental Conditions ◽  
Insertion Depth ◽  
Retention Capacity ◽  
Soil Layering ◽  
Soil Hydraulic ◽  
The Impact ◽  
Infiltration Experiment

AbstractPonded infiltration experiment is a simple test used for in-situ determination of soil hydraulic properties, particularly saturated hydraulic conductivity and sorptivity. It is known that infiltration process in natural soils is strongly affected by presence of macropores, soil layering, initial and experimental conditions etc. As a result, infiltration record encompasses a complex of mutually compensating effects that are difficult to separate from each other. Determination of sorptivity and saturated hydraulic conductivity from such infiltration data is complicated. In the present study we use numerical simulation to examine the impact of selected experimental conditions and soil profile properties on the ponded infiltration experiment results, specifically in terms of the hydraulic conductivity and sorptivity evaluation. The effect of following factors was considered: depth of ponding, ring insertion depth, initial soil water content, presence of preferential pathways, hydraulic conductivity anisotropy, soil layering, surface layer retention capacity and hydraulic conductivity, and presence of soil pipes or stones under the infiltration ring. Results were compared with a large database of infiltration curves measured at the experimental site Liz (Bohemian Forest, Czech Republic). Reasonably good agreement between simulated and observed infiltration curves was achieved by combining several of factors tested. Moreover, the ring insertion effect was recognized as one of the major causes of uncertainty in the determination of soil hydraulic parameters.

scholarly journals EMBRYONIC DEVELOPMENT OF CLARIAS BATRACHUS UNDER THE INFLUENCE OF AERATION AND WATER FLOW

2013 ◽  
Vol 18 ◽  
pp. 25-31
Author(s):  
Sk. Mustafizur Rahman ◽  
Md. Ahasan Habib ◽  
Quazi Zahangir Hossain ◽  
Md. Noman Siddiqui ◽  
Md. Mostafizur Rahman ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Water Flow ◽  
Developmental Stages ◽  
Cost Effective ◽  
Clarias Batrachus ◽  
Experimental Conditions ◽  
Early Developmental Stages ◽  
Time Requirements ◽  
Early Developmental

Specific investigation on the time (h) required for catfish, Clarias batrachus, embryos to reach various early developmental stages were carried out under different experimental conditions. Five hundred fertilized eggs were incubated in tray (28ºC–30.5ºC) with continuous water flow and aeration (T1), with only continuous water flow (T2) or aeration (T3) and without continuous water flow and aeration (T4). Fertilized embryos hatched successfully in all treatments except for T4. Time requirements for 2-cell, 4-cell, 8-cell, morula, germinal ring, yolk-plug, twisting movement, prehatching and hatching were comparable for all treatments. However, developmental speed was ceased from yolk-plug stage for T4. Time requirements for two-cell, four-cell, eight-cell, morula, germinal ring, yolk-plug, twisting movement, and hatching were 0.43, 0.48, 0.53, 3.52, 6.07, 8.50, 15.04, 20.08, and 21.40 h, respectively for T1; 0.44, 0.47, 0.51, 3.49, 6.10, 8.51, 15.13, 20.19, and 21.56 h, respectively for T2; 0.50, 0.53, 0.59, 3.52, 6.13, 8.53, 15.11, 20.18, and 22.13 h, respectively for T3. Fertilized eggs of C. batrachus incubated in tray with only aeration (T3) is sufficient for successful hatching and could be cost-effective for hatchery operation.DOI: http://dx.doi.org/10.3126/eco.v18i0.9395Ecoprint: An International Journal of EcologyVol. 18, 2011 Page: Uploaded date: 12/20/2013 

scholarly journals Assessment of the impact wastewater of dairy farms on soil microbiota

2021 ◽  
pp. 113-118
Author(s):  
Ye. Kryvokhyzha
Keyword(s):  
Dairy Farms ◽  
Cattle Breeding ◽  
Soil Microbiota ◽  
Internal Surfaces ◽  
Number Of Microorganisms ◽  
Negative Effect ◽  
Breeding Farms ◽  
The Impact ◽  
Different Doses

In the production of milk on cattle-breeding farms and complexes large amount of wastewater is formed containing significant numbers of chemicals and is ecologically hazardous source of pollution of water and soil resources. Wastewater of dairy farms contain natural animal excretions, exhaust solutions of cleaning and disinfecting agents, milk residue which is washed off from internal surfaces of milking equipment, residues of feed, litter, dirt, which is washed off during washing of walls and floor of dairy cow house. Since such wastewater contain valuable organic substances they are used to fertilize plants. There are technologies of safe use of wastewater from cattle-breeding farms to fertilize pastures. Also wastewater used as fertilizer in cultivation of sugar beets, tomatoes, cucumbers, spinach, cabbage and the like. It is expedient determination of the influence of wastewater used for the fertilizer of agricultural cultures, on the soil microbiota. After all, soil fertility is inextricably linked with microorganisms that inhabit her. The aim of the work was carrying out of an assessment of influence of wastewater from dairy farms on the soil microbiota. Samples of soil for microbiological studies were selected in 20 centimetre thickness of top layer after application of wastewater of dairy farms. The total microorganism number was determined the method of sowing of soil suspension of corresponding dilutions on nutrient medium of meat-and-peptone agar (MPA). Micromycetes on Chapek medium.  The state of soil microorganisms after application in her different doses of wastewater of dairy farms was investigated. It is established, that the total number of microorganisms in soil when accounting 12 days later after application of wastewater in quantities of 500 l/ha exceeded control by 7.4%. At doses of 600 l/ha and 700 l/ha by 9.8% and 12.4% accordingly. 30 days later after application of waste-water the number of microorganisms almost did not differ from control. It was observed that after application of wastewater increase the total number of micromycetes. 12 days later after application of dose 500 l/ha,  of number of micromycetes was increased on average by 14,6%; in dose of 600 l/ha by 18.0%; in dose of 700 l/ha — by 19.6%. 30 days later after application of wastewater the total number of micromycetes exceeded control by 1.5%. It is defined, that wastewater of dairy farms in doses of 500–700 l/ha do not exert negative effect on soil microbiota.

Does Unconscious Information Affect Cognitive Activity?: A Study Using Experimental Priming

2011 ◽  
Vol 14 (1) ◽  
pp. 20-36 ◽  
Author(s):  
Margarita G. Filippova
Keyword(s):  
Information Processing ◽  
Priming Effect ◽  
Choice Model ◽  
Cognitive Activity ◽  
Processing System ◽  
Spreading Activation ◽  
Experimental Conditions ◽  
Priming Paradigm ◽  
The Impact

In a series of three experiments the influence that information unrecognised by the subjects has on the effectiveness of occurring cognitive activity is studied. With this aim 3 types of stimulus were compared which for one reason or another were not afforded sufficient attention, namely: unconscious meanings of polysemantic information, stimuli presented at the subliminal level, and intentionally ignored distractors. All the listed types of stimuli are united in that the subjects were not able to give an account of them, i.e., these stimuli were not processed attentively. It is assumed that each of the types of stimuli studied is in actuality perceived, which can be judged by the impact they have on occurring cognitive activity. The purpose of the present research is the comparison of this impact: apart from the determination of the impact of unperceived stimuli on the information directly associated with them (priming-effect registration), also identified is the presence/absence of an overall interference effect rendered by the unperceived stimuli on the performance of occurring cognitive activity. To this end, each experiment had a control condition the aim of which was the creation of the possibility for the subjects to perceive stimuli unnoticed under experimental conditions. An experimental priming paradigm was used in combination with image-classification and lexical-decision tasks.The results of the experiments conducted demonstrate that all types of stimuli ‘slipping the attention’ are assimilated, but their effect on occurring cognitive activity is varied. Thus, subliminally presented information aids, and distractors, on the contrary, hinder the solution of tasks associated with them, whereas unperceived meanings of polysemantic information hinder not only the solution of the tasks directly associated with them, but also the performance of any other cognitive activity for which they serve as a context. The effect of subliminal stimuli on occurring cognitive activity in the present research is explained by the spreading activation in the memory, the effect of distractors – by the inhibition of irrelevant representations in the information-processing system. For an explanation of the consequence of unperceived meanings of polysemanticity, not only an inhibition model was used, but also an unconscious negative choice model which assumed the necessity of making a special decision on non-perception.

scholarly journals Toxicity of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NKK) in early development: a wide-scope metabolomics assay in zebrafish embryos

2021 ◽  
Author(s):  
Carla Merino ◽  
Marta Casado ◽  
Benjami Pina ◽  
Maria Vinaixa ◽  
Noelia Ramirez
Keyword(s):  
Early Development ◽  
Developmental Stages ◽  
Excision Repair ◽  
Environmental Pollutant ◽  
Zebrafish Embryos ◽  
Wide Scope ◽  
Base Excision ◽  
Mammalian Toxicity ◽  
The Impact ◽  
Early Developmental

The tobacco-specific nitrosamine 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a carcinogenic and ubiquitous environmental pollutant which carcinogenic and cytotoxic activity has been thoroughly investigated in murine models and human tissues. However, its potential deleterious effects on vertebrate early development are yet poorly understood. In this work, we characterized the impact of NNK exposure during early developmental stages of zebrafish embryos, a known alternative model for mammalian toxicity studies. Embryos exposed to different NNK concentrations were monitored for lethality and for the appearance of malformations during the first five days after fertilization. LC/MS-based untargeted metabolomics was subsequently performed for a wide-scope assay of NNK-related metabolic alterations. Our results revealed the presence of not only the parental compound, but also of two NKK known metabolites, 4-Hydroxy-4-(3-pyridyl)-butyric acid (HPBA) and 4-(Methylnitrosamino)-1-(3-pyridyl-N-oxide)-1-butanol (NNAL-N-oxide) in exposed embryos likely resulting from active CYP450-mediated α-hydroxylation and NNK detoxification pathways, respectively. This was paralleled by a disruption in purine and pyrimidine metabolisms and the activation of the base excision repair pathway. Our results confirm NNK as a harmful embryonic agent and demonstrate zebrafish embryos to be a suitable early development model to monitor NNK toxicity.

The impact of salinity on early developmental stages in two sympatric spadefoot toads and implications for amphibian conservation in coastal areas

Hydrobiologia ◽  
2016 ◽  
Vol 792 (1) ◽  
pp. 357-366 ◽  
Author(s):  
Florina Stănescu ◽  
Diana Székely ◽  
Paul Székely ◽  
Sebastian Topliceanu ◽  
Dan Cogălniceanu
Keyword(s):  
Developmental Stages ◽  
Coastal Areas ◽  
Amphibian Conservation ◽  
Spadefoot Toads ◽  
Early Developmental Stages ◽  
The Impact ◽  
Early Developmental

scholarly journals Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality

2019 ◽  
Vol 24 (5) ◽  
pp. 515-522 ◽  
Author(s):  
Shane M. Buker ◽  
P. Ann Boriack-Sjodin ◽  
Robert A. Copeland
Keyword(s):  
Drug Discovery ◽  
Substrate Concentration ◽  
Chemical Biology ◽  
Enzyme Inhibitor ◽  
Specific Inhibition ◽  
Experimental Conditions ◽  
The Common ◽  
Inhibitory Molecules ◽  
The Impact

Contemporary chemical biology and drug discovery are increasingly focused on the discovery of inhibitory molecules that interact with enzyme targets in specific ways, such as allosteric or orthosteric binding. Hence, there is increasing interest in evaluating hit compounds from high-throughput diversity screening to determine their mode of interaction with the target. In this work, the common inhibition modalities are reviewed and clarified. The impact of substrate concentration, relative to substrate KM, for each common inhibition modality is also reviewed. The pattern of changes in IC50 that accompany increasing substrate concentration are shown to be diagnostic of specific inhibition modalities. Thus, replots of IC50 as a function of the ratio [S]/KM are recommended as a simple and rapid means of assessing inhibition modality. Finally, specific recommendations are offered for ideal experimental conditions for the determination of inhibition modality through the use of IC50 replots.

scholarly journals Development of a new procedure for the determination of the reactivity of brandies used in wine fortification

OENO One ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 161-172
Author(s):  
Joana Azevedo ◽  
João Pissarra ◽  
Filipa Amaro ◽  
Luis Guido ◽  
Joana Oliveira ◽  
...  
Keyword(s):  
Reaction Time ◽  
Coefficient Of Variation ◽  
Condensed Tannins ◽  
New Method ◽  
Reactivity Index ◽  
Visible Spectroscopy ◽  
Experimental Conditions ◽  
The Impact ◽  
Reproducibility Limit

The main purpose of this work was to describe and validate a new methodology for the determination of a brandy reactivity index (BRI) towards flavan-3-ol compounds in wines (catechins and condensed tannins) and to correlate this index with the amount and type of aldehydes present in those brandies. This new method consists of two main reactions: a) the ability of aldehydes to react with C8 and/or C6 positions in the phloroglucinol ring of a catechin present in excess (A ring); b) further reaction of the remaining free catechin with p-dimethylaminocinamaldehyde (DMACA) yielding to the formation of a blue compound that can be quantified by Visible spectroscopy at 640 nm. The impact of different experimental conditions such as reagent concentrations and reaction time on BRI were also evaluated. The method was validated through the determination of repeatability (intra-day variability) and reproducibility. The repeatability was considered acceptable with a CV of 11.87 %. The analysis of the reproducibility variance, S2R (11.59), the reproducibility limit R (9.5) and the reproducibility coefficient of variation, CVR (15.25 %) postulates BRI methodology to be reliable and robust.Using the developed methodology, the BRI of fourteen different commercial brandies and some pure standards aldehydes were determined. The aldehydes present in brandies, and the content of total aldehydes were determined by GC-MS and then correlated with the BRI. In general, it was observed a direct correlation between the BRI and the concentration of total aldehydes, in particular acetaldehyde.

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