scholarly journals EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL

2021 ◽  
pp. 9
Author(s):  
Keyword(s):  
Plasma Membrane ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Positive Control ◽  
Experimental Models ◽  
Molecular Characteristics ◽  
Pharmacological Activities ◽  
Hemolysis Assay ◽  
Toxicological Studies ◽  
Hemolytic Action

Yangambin, a lignan isolated from the leaves of Ocotea duckei Vattimo-Gil, has several pharmacological activities described in literature. However, few information about its toxicity has been reported. Red cells represent about 90% of blood cells and have interesting structural and molecular characteristics as experimental models in toxicological studies. Thus, the aim of this study was to evaluate the hemolytic action of yangambin in sheep blood. The hemolysis assay was performed at concentrations of 50, 25 and 12.5 µg/mL of yangambin in triplicate and hemolysis percentage was defined through the absorbance resulting from the test concentrations compared to the positive control. The results showed that yangambin did not cause hemolysis at the concentrations tested, therefore it did not cause damage to the plasma membrane of sheep erythrocytes.

scholarly journals In Vitro Studies on the Anemia of Tumor-Bearing Hamsters

Blood ◽  
1960 ◽  
Vol 15 (1) ◽  
pp. 130-136 ◽  
Author(s):  
JOSEPH D. SHERMAN ◽  
CARMEN RICKARD ◽  
ROBERT S. CHRISTIAN ◽  
GILBERT H. FRIEDELL
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
In Vitro Studies ◽  
Necrotic Tissue ◽  
Sterile Cell ◽  
Tumor Bearing ◽  
Hemolytic Action

Abstract 1. Sterile, cell-free extracts of the viable portion of a methylcholanthrene-induced sarcoma of the hamster were capable of hemolyzing in vitro hamster red blood cells from the donor animals, and from animals with homologous and heterologous tumors. 2. Sterile, cell-free extracts of the necrotic material from this same tumor had little in vitro hemolytic action. 3. Whole tumor extracts varied in their in vitro hemolytic activity depending upon the proportion of viable to necrotic tissue present, with the maximum hemolysis observed when the whole tumor contained more viable than necrotic tissue. 4. Sterile, cell-free extracts of normal hamster liver had a strong hemolytic action on a whole range of red blood cells. 5. Hemolysins elaborated by the viable tissue in transplanted hamster tumors may be one factor contributing to the anemia in hamsters bearing transplantable sarcomas.

scholarly journals Emerging cellular and molecular mechanisms underlying anticancer indications of chrysin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Marjan Talebi ◽  
Mohsen Talebi ◽  
Tahereh Farkhondeh ◽  
Jesus Simal-Gandara ◽  
Dalia M. Kopustinskiene ◽  
...  
Keyword(s):  
Blood Cells ◽  
Molecular Mechanisms ◽  
Web Of Science ◽  
Experimental Studies ◽  
Protective Effects ◽  
Pharmacological Activities ◽  
Current Review ◽  
Mesh Terms ◽  
Online Databases ◽  
Anti Cancer

AbstractChrysin has been shown to exert several beneficial pharmacological activities. Chrysin has anti-cancer, anti-viral, anti-diabetic, neuroprotective, cardioprotective, hepatoprotective, and renoprotective as well as gastrointestinal, respiratory, reproductive, ocular, and skin protective effects through modulating signaling pathway involved in apoptosis, oxidative stress, and inflammation. In the current review, we discussed the emerging cellular and molecular mechanisms underlying therapeutic indications of chrysin in various cancers. Online databases comprising Scopus, PubMed, Embase, ProQuest, Science Direct, Web of Science, and the search engine Google Scholar were searched for available and eligible research articles. The search was conducted by using MeSH terms and keywords in title, abstract, and keywords. In conclusion, experimental studies indicated that chrysin could ameliorate cancers of the breast, gastrointestinal tract, liver and hepatocytes, bladder, male and female reproductive systems, choroid, respiratory tract, thyroid, skin, eye, brain, blood cells, leukemia, osteoblast, and lymph. However, more studies are needed to enhance the bioavailability of chrysin and evaluate this agent in clinical trial studies. Graphic abstract

Probing the extracellular release site of the plasma membrane calcium pump

2000 ◽  
Vol 278 (5) ◽  
pp. C965-C972 ◽  
Author(s):  
Wanyan Xu ◽  
Betty Jo Wilson ◽  
Lin Huang ◽  
Emma L. Parkinson ◽  
Brent J. F. Hill ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Blood Cells ◽  
High Field ◽  
Release Site ◽  
Calcium Pump ◽  
Maximal Inhibition ◽  
Human Red Blood Cells ◽  
Access Channel ◽  
Extracellular Release ◽  
Better Than

The plasma membrane Ca2+ pump is known to mediate Ca2+/H+ exchange. Extracellular protons activated 45Ca2+ efflux from human red blood cells with a half-maximal inhibition constant of 2 nM when the intracellular pH was fixed. An increase in pH from 7.2 to 8.2 decreased the IC50 for extracellular Ca2+ from ∼33 to ∼6 mM. Changing the membrane potential by >54 mV had no effect on the IC50 for extracellular Ca2+. This argues against Ca2+ release through a high-field access channel. Extracellular Ni2+ inhibited Ca2+ efflux with an IC50 of 11 mM. Extracellular Cd2+ inhibited with an IC50 of 1.5 mM, >10 times better than Ca2+. The Cd2+ IC50 also decreased when the pH was raised from 7.1 to 8.2, consistent with Ca2+, Cd2+, and H+ competing for the same site. The higher affinity for inhibition by Ni2+and Cd2+ is consistent with a histidine or cysteine as part of the release site. The cysteine reagent 2-(trimethylammonium)ethyl methanethiosulfonate did not inhibit Ca2+ efflux. Our results are consistent with the notion that the release site contains a histidine.

scholarly journals The plasma membrane Ca2+-ATPase protein from red blood cells is not modified in preeclampsia

2006 ◽  
Vol 1762 (3) ◽  
pp. 381-385 ◽  
Author(s):  
Néstor J. Oviedo ◽  
Gustavo Benaim ◽  
Vincenza Cervino ◽  
Teresa Proverbio ◽  
Fulgencio Proverbio ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Red Blood Cells ◽  
Blood Cells

Nanotoxicity of Silver Nanoparticles to Red Blood Cells: Size Dependent Adsorption, Uptake, and Hemolytic Activity

2015 ◽  
Vol 28 (3) ◽  
pp. 501-509 ◽  
Author(s):  
Li Qiang Chen ◽  
Li Fang ◽  
Jian Ling ◽  
Cheng Zhi Ding ◽  
Bin Kang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Size Dependent

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

scholarly journals Isolation and properties of glycophorin from plasma membrane of hen red blood cells

1996 ◽  
Vol 12 (4) ◽  
pp. 94-99 ◽  
Author(s):  
T. V. Stasyk ◽  
M. D. Lutsik-Kordovsky
Keyword(s):  
Plasma Membrane ◽  
Red Blood Cells ◽  
Blood Cells

scholarly journals Perfluoroalkyl and polyfluoroalkyl substances (PFAS) and their effects on the ovary

2020 ◽  
Vol 26 (5) ◽  
pp. 724-752 ◽  
Author(s):  
Ning Ding ◽  
Siobán D Harlow ◽  
John F Randolph Jr ◽  
Rita Loch-Caruso ◽  
Sung Kyun Park
Keyword(s):  
Human Population ◽  
Ovarian Function ◽  
Experimental Studies ◽  
Epidemiologic Studies ◽  
Experimental Models ◽  
Human Populations ◽  
Reverse Causality ◽  
Toxicological Studies ◽  
Polyfluoroalkyl Substances ◽  
Perfluoroalkyl And Polyfluoroalkyl Substances

Abstract BACKGROUND Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are found widespread in drinking water, foods, food packaging materials and other consumer products. Several PFAS have been identified as endocrine-disrupting chemicals based on their ability to interfere with normal reproductive function and hormonal signalling. Experimental models and epidemiologic studies suggest that PFAS exposures target the ovary and represent major risks for women’s health. OBJECTIVE AND RATIONALE This review summarises human population and toxicological studies on the association between PFAS exposure and ovarian function. SEARCH METHODS A comprehensive review was performed by searching PubMed. Search terms included an extensive list of PFAS and health terms ranging from general keywords (e.g. ovarian, reproductive, follicle, oocyte) to specific keywords (including menarche, menstrual cycle, menopause, primary ovarian insufficiency/premature ovarian failure, steroid hormones), based on the authors’ knowledge of the topic and key terms. OUTCOMES Clinical evidence demonstrates the presence of PFAS in follicular fluid and their ability to pass through the blood–follicle barrier. Although some studies found no evidence associating PFAS exposure with disruption in ovarian function, numerous epidemiologic studies, mostly with cross-sectional study designs, have identified associations of higher PFAS exposure with later menarche, irregular menstrual cycles, longer cycle length, earlier age of menopause and reduced levels of oestrogens and androgens. Adverse effects of PFAS on ovarian folliculogenesis and steroidogenesis have been confirmed in experimental models. Based on laboratory research findings, PFAS could diminish ovarian reserve and reduce endogenous hormone synthesis through activating peroxisome proliferator-activated receptors, disrupting gap junction intercellular communication between oocyte and granulosa cells, inducing thyroid hormone deficiency, antagonising ovarian enzyme activities involved in ovarian steroidogenesis or inhibiting kisspeptin signalling in the hypothalamus. WIDER IMPLICATIONS The published literature supports associations between PFAS exposure and adverse reproductive outcomes; however, the evidence remains insufficient to infer a causal relationship between PFAS exposure and ovarian disorders. Thus, more research is warranted. PFAS are of significant concern because these chemicals are ubiquitous and persistent in the environment and in humans. Moreover, susceptible groups, such as foetuses and pregnant women, may be exposed to harmful combinations of chemicals that include PFAS. However, the role environmental exposures play in reproductive disorders has received little attention by the medical community. To better understand the potential risk of PFAS on human ovarian function, additional experimental studies using PFAS doses equivalent to the exposure levels found in the general human population and mixtures of compounds are required. Prospective investigations in human populations are also warranted to ensure the temporality of PFAS exposure and health endpoints and to minimise the possibility of reverse causality.

scholarly journals ON THE RELATION OF WHITE BLOOD CELLS AND PLATELETS TO VENOM HEMOLYSIS

1956 ◽  
Vol 104 (4) ◽  
pp. 517-523 ◽  
Author(s):  
Joseph C. Turner ◽  
Keyword(s):  
Guinea Pig ◽  
Hemolytic Activity ◽  
Chromatographic Separation ◽  
Blood Cells ◽  
Direct Action ◽  
White Blood Cells ◽  
Substantial Reduction ◽  
Red Cells ◽  
Phospholipase Activity ◽  
Paper Chromatographic Separation

Removal of the white cells and platelets from suspensions of red cells usually produces substantial reduction in the hemolytic activity of venoms. Guinea pig red cells constitute a notable exception and may be lysed by a direct action of venom. White blood cells and platelets appear to contribute to hemolysis by serving as sources of phosphatides for the formation of lysophosphatides. No correlation could be found between phospholipase activity and direct hemolytic activity of venoms. A recently described method (8) of paper chromatographic separation of phospholipides has been used successfully in part of the work.

Sign in / Sign up

Export Citation Format

Share Document