DEMONSTRATION OF THE HUMAN HAIR FOLLICLE MAGNETORECEPTION OF BIOMAGNETISM RADIATED BY THE CONCAVE PART OF THE HUMAN HAND

Biological material has been documented to produce an external magnetic field that radiates out. There have been several papers documenting the magnetic fields produced by steady currents in the body. The most notable was published in 1980 by Cohen et al. where the human hair follicle was used as sentinel and biophysically evaluated via sophisticated equipment such as a double planar Superconducting Quantum Interference Devices (SQUID). Most recently, in 2019 Cohen’s work was duplicated by Khan,S by also using double-planar gladiometers. Of interest to this manuscript is that since the introduction of anovel optical microscopy method in 2016 by Scherlag BJ et al is that numerous papers have been introduced in the literature now identifying intrinsic biomagnetic properties of the follicle such as penetration through glass barriers. In this manuscript, a concept of biomagnetic fields by the concave part of the human hand transferring energy to hair follicles is introduced, this was accomplished by using a novel optical microscopy method, in other words, the hair follicle is not limited to radiate out biomagnetism; but also, to receive externally radiated biomagnetic fields from a body part. This magneto receptive property is herein introduced.

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A method for culturing human hair follicle cells. (II). Plucked human hair follicles.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.50.271 ◽

1988 ◽

Vol 50 (2) ◽

pp. 271-276 ◽

Cited By ~ 8

Author(s):

Ryuichiro KUWANA ◽

Seiji ARASE ◽

Yasushi SADAMOTO ◽

Hideki NAKANISHI ◽

Katsuyuki TAKEDA

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Hair Follicles ◽

Follicle Cells ◽

Human Hair Follicle

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Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle

Histochemistry and Cell Biology ◽

10.1007/s00418-020-01957-8 ◽

2021 ◽

Author(s):

Megan A. Palmer ◽

Eleanor Smart ◽

Iain S. Haslam

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Vital Role ◽

Transport Proteins ◽

Hair Follicles ◽

Regulatory Control ◽

Cholesterol Transport ◽

Hair Cycle ◽

Human Hair Follicle

AbstractCholesterol has long been suspected of influencing hair biology, with dysregulated homeostasis implicated in several disorders of hair growth and cycling. Cholesterol transport proteins play a vital role in the control of cellular cholesterol levels and compartmentalisation. This research aimed to determine the cellular localisation, transport capability and regulatory control of cholesterol transport proteins across the hair cycle. Immunofluorescence microscopy in human hair follicle sections revealed differential expression of ATP-binding cassette (ABC) transporters across the hair cycle. Cholesterol transporter expression (ABCA1, ABCG1, ABCA5 and SCARB1) reduced as hair follicles transitioned from growth to regression. Staining for free cholesterol (filipin) revealed prominent cholesterol striations within the basement membrane of the hair bulb. Liver X receptor agonism demonstrated active regulation of ABCA1 and ABCG1, but not ABCA5 or SCARB1 in human hair follicles and primary keratinocytes. These results demonstrate the capacity of human hair follicles for cholesterol transport and trafficking. Future studies examining the role of cholesterol transport across the hair cycle may shed light on the role of lipid homeostasis in human hair disorders.

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Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice

International Journal of Stem Cells ◽

10.15283/ijsc.2008.1.1.70 ◽

2008 ◽

Vol 1 (1) ◽

pp. 70-81 ◽

Cited By ~ 4

Author(s):

Sung-Hoon Lee ◽

Mi-Kyung Chung ◽

Yong-Jin Sohn ◽

Yong-Soon Lee ◽

Kyung-Sun Kang

Keyword(s):

Cell Surface ◽

Hair Follicle ◽

Nude Mice ◽

Human Hair ◽

Hair Follicles ◽

Surface Marker ◽

Follicle Cells ◽

Human Hair Follicle ◽

Outer Root Sheath ◽

Root Sheath

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Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix

International Journal of Molecular Sciences ◽

10.3390/ijms20071741 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1741 ◽

Cited By ~ 1

Author(s):

Sandra Fernández-Martos ◽

María Calvo-Sánchez ◽

Karla García-Alonso ◽

Begoña Castro ◽

Bita Hashtroody ◽

...

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Wound Repair ◽

Ex Vivo ◽

Hair Follicles ◽

Stem Cell Markers ◽

Follicle Growth ◽

Human Hair Follicle ◽

Hydrogel Matrix ◽

Hair Follicle Growth

Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in homeostatic maintenance and regenerative processes (e.g., wound repair) of the skin. However, little is known about the role of these molecules in the regulation of the hair follicle cycle. Here we report that growing human hair follicles ex vivo in a defined GAG hydrogel mimicking the dermal matrix strongly promotes sustained cell survival and maintenance of a highly proliferative phenotype in the hair bulb and suprabulbar regions. This significant effect is associated with the activation of WNT/β-catenin signaling targets (CCDN1, AXIN2) and with the expression of stem cell markers (CK15, CD34) and growth factors implicated in the telogen/anagen transition (TGFβ2, FGF10). As a whole, these results point to the dermal GAG matrix as an important component in the regulation of the human hair follicle growth cycle, and to GAG-based hydrogels as potentially relevant modulators of this process both in vitro and in vivo.

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Ectodysplasin-A2 induces apoptosis in cultured human hair follicle cells and promotes regression of hair follicles in mice

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2019.10.031 ◽

2019 ◽

Vol 520 (2) ◽

pp. 428-433 ◽

Cited By ~ 3

Author(s):

Mi Hee Kwack ◽

Jung Chul Kim ◽

Moon Kyu Kim

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Hair Follicles ◽

Follicle Cells ◽

Human Hair Follicle

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LARGE-SCALE EXPANSION AND CHARACTERIZATION OF HUMAN ADULT NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS FROM HAIR FOLLICLE FOR REGENERATIVE MEDICINE APPLICATIONS

Experimental Oncology ◽

10.31768/2312-8852.2017.39(3):171-180 ◽

2017 ◽

Vol 39 (3) ◽

pp. 171-180 ◽

Cited By ~ 4

Author(s):

R G Vasyliev ◽

A E Rodnichenko ◽

O S Gubar ◽

A V Zlatska ◽

I M Gordiienko ◽

...

Keyword(s):

Stem Cells ◽

Neural Crest ◽

Hair Follicle ◽

Human Hair ◽

Large Scale ◽

Hair Follicles ◽

Human Hair Follicle ◽

Self Renewal ◽

Multipotent Stem Cells ◽

Clonogenic Potential

Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2+Sox10+Nestin+CD73+CD90+CD105+CD140a+CD 140b+CD146+CD166+CD271+CD349+ CD34-CD45-CD56-HLA-DR-, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.

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Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells

Current Issues in Molecular Biology ◽

10.3390/cimb43030097 ◽

2021 ◽

Vol 43 (3) ◽

pp. 1361-1373

Author(s):

Jae Young Shin ◽

Jaeyoon Kim ◽

Yun-Ho Choi ◽

Nae-Gyu Kang ◽

Sanghwa Lee

Keyword(s):

Cell Viability ◽

Hair Follicle ◽

Human Hair ◽

Cellular Proliferation ◽

Hair Follicles ◽

Cell Senescence ◽

Follicle Cells ◽

Human Hair Follicle ◽

Outer Root Sheath ◽

Anagen Phase

Dexpanthenol (D-panthenol) is a precursor of vitamin B5 (pantothenic acid) and is widely used for dietary supplements and topical applications. D-panthenol has long been used in hair care products for the purpose of anti-hair loss, its effects and the underlying mechanisms, however, were barely reported. In this study, the effects of D-panthenol on human hair follicle cells, including dermal papilla cells (hDPCs) and outer root sheath cells (hORSCs), were investigated. D-panthenol enhanced the cell viability, increasing the cellular proliferation marker Ki67 in cultured hDPCs. The markers for apoptosis (Caspase3/9) and cell senescence (p21/p16), reported to be expressed in aged or resting phase follicles, were significantly reduced by D-panthenol. Anagen-inducing factors (ALP; β-catenin; versican), which trigger or elongate the anagen phase, were stimulated by D-panthenol. On the other hand, D-panthenol reduced TGF-β1 expressions in both mRNA and protein levels. The expression of VEGF, which is important for peripheral blood vessel activation; was up-regulated by D-panthenol treatment. In cultured hORSCs, cell proliferation and viability were enhanced, while the mRNA expression of cell senescence markers (p21/p16) was significantly down-regulated. The expressions of both VEGF and its receptor (VEGFR) were up-regulated by D-panthenol. In conclusion, our data suggest that the hair growth stimulating activity of D-panthenol was exerted by increasing the cell viability, suppressing the apoptotic markers, and elongating the anagen phase in hair follicles.

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THE HUMAN HAIR FOLLICLE PULSATING BIOMAGNETIC FIELD REACH AS MEASURED BY CRYSTALS ACCRETION

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v6.i7.2018.1309 ◽

2018 ◽

Vol 6 (7) ◽

pp. 290-299 ◽

Cited By ~ 1

Author(s):

Abraham A.

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Hair Follicles ◽

Iron Particles ◽

Human Hair Follicle ◽

Microscopy Technique ◽

Video Recordings ◽

Glass Slides ◽

Blue Stain ◽

Fine Iron

This manuscript introduces the biomagnetic fields reach (BMFs) of the human hair follicles. The introduction of a novel table top optical microscopy technique using a special Prussian Blue Stain solution (PBS) mixed with fine iron particles has produced numerous papers confirming the inherent biomagnetism of the human hair. This technique allowed for the design of sets of incremental stacked glass slides for the purpose of measuring the human hair follicle BMFs reach out. This was demonstrated (measured) by using diamagnetic as well as paramagnetic Potassium Ferrocyanide preparations mixed with fine iron particles. Still microphotographs and video-recordings are presented.

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THE HUMAN HAIR FOLLICLE PULSATING BIOMAGNETIC FIELD REACH AS POSSIBLE ADDITIONAL FACTOR IN MIGRAINE HEADACHES A BIOPHYSICS BASED HYPOTHESIS

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v8.i5.2020.179 ◽

2020 ◽

Vol 8 (5) ◽

pp. 221-229

Author(s):

Abraham A. Embi

Keyword(s):

Hair Follicle ◽

Electromagnetic Fields ◽

Human Hair ◽

Hair Follicles ◽

Pulsed Electromagnetic Fields ◽

Iron Particles ◽

Human Hair Follicle ◽

Migraine Headaches ◽

Microscopy Technique ◽

Pulsed Electromagnetic

This manuscript introduces a hypothesis linking the intrinsic pulsating nature of the biomagnetic fields reach found in the human hair follicle as factor in the etiology of migraine headaches. In the last two decades, researchers have emphasized the efficiency of external pulsed electromagnetic fields in the treatment of migraine headaches. Clinical trials have also demonstrated that external pulsed electromagnetic fields may prevent or decrease the migraine attacks. A hypothesis is presented linking the inherent hair follicle pulsed bioelectomagnetism as a factor in the etiology of migraines. Does the internal pulsed biomagnetic field reach of the hair follicles factor in the genesis of migraine headaches? Supporting the hypothesis are published papers confirming the inherent biomagnetism of the human hair follicle. The introduction of a novel optical microscopy technique using a special Prussian Blue Stain (PBS) mixed with fine iron particles has produced numerous papers confirming the inherent biomagnetism of the human hair. This manuscript expands on those findings by introducing documentation of the hair follicle pulsating biomagnetic field reach. This is demonstrated by using diamagnetic as well as paramagnetic preparations mixed with iron particles. Still microphotographs and video-recordings are presented.

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