scholarly journals CHARACTERIZATION OF PREDICTED BACTERIAL COLD-ADAPTED LIPASE FROM SEAFOOD COLD STORAGE

2022 ◽  
Vol 9 (12) ◽  
pp. 242-250
Author(s):  
Hefti Salis Yufidasari ◽  
Retno Tri Astuti ◽  
Eko Waluyo ◽  
Jekmal Malau
Keyword(s):  
Cold Storage ◽  
Unique Feature ◽  
Lipolytic Activity ◽  
Production Costs ◽  
Addition Reactions ◽  
Optimum Temperature ◽  
Cold Temperatures ◽  
Cold Adapted ◽  
Lower Production

Lipases constitute as top three most important group of enzymes along with  carbohydrases and proteases, and are widely used in various industries. In particular, lipase that perform high activity at low temperatures, or referred as cold adapted lipase (CLPs) considered as attractive catalyst due to its activity at low temperature. This unique feature is the main advantage of cold adapted lipase utilization because it requires a low energy source that is correlated with lower production costs and energy. In addition, reactions occur in cold temperatures may result in better product quality. The purpose of this research was to perform screening and characterization of bacterial cold adapted lipase from seafood cold storage. Among 53 isolates, Kr_16_30, TI_37_14 and Kr_16_28 showed the highest activity with 4.12 U/mL; 3.87 U/mL and 3.21 U/mL, respectively. Isolates Kr_16_30 seemed to be typical cold adapted lipase with optimum temperature at 20°C and pH 7. Isolates Kr_16_28 performed highest lipolytic activity at 30°C while TI_37_14 suspected to be similar to typical mesophilic lipase with optimum temperature at 40°C. Species identification based on 16s rRDA sequencing revealed that isolates Kr_16 30 and Kr_16 28 are belong to genus Pseudomonas and Bacillus, repectively.

scholarly journals Characterization of EstDR4, a Novel Cold-Adapted Insecticides-Metabolizing Esterase from Deinococcus radiodurans

2021 ◽  
Vol 11 (4) ◽  
pp. 1864
Author(s):  
Yage Zhang ◽  
Wei Lu ◽  
Jin Wang ◽  
Ming Chen ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Deinococcus Radiodurans ◽  
Tween 80 ◽  
Pharmaceutical Products ◽  
High Catalytic Activity ◽  
Biotechnological Applications ◽  
Optimum Temperature ◽  
Initial Activity ◽  
Cold Adapted ◽  
Triton X

Cold-adapted esterases are attracting increasing attention owing to their prospective use in biotechnology. In this study, a novel cold-adapted family Ⅳ esterase EstDR4 was identified and obtained from extremophile Deinococcus radiodurans (D. radiodurans). EstDR4 displayed significant substrate preference towards short and medium chain monoesters (C2–C12). It also showed regioselectivity, enantioselectivity and degradation effects on four insecticides. The optimum temperature and pH for EstDR4 activity were 30 °C and pH 8, respectively. Additionally, EstDR4 exhibited relatively high catalytic activity at 0 °C and high stability from 10–40 °C, with over 80% of its initial activity retained after 1 h of incubation. Moreover, EstDR4 activity was stimulated by Tween 80 and Triton X-100, and inhibited by metal ions such as Co2+, Cu2+ and Zn2+ and several organic solvents. Thus, this enzyme shows development potential for many industrial biotechnological applications, including the manufacture of thermolabile pharmaceutical products, cold-wash detergents and insecticide biodegradation.

scholarly journals Time-Resolved Spectroscopic Study of N,N-Di(4-bromo)nitrenium Ions in Selected Solutions

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3182 ◽  
Author(s):  
Lili Du ◽  
Xin Lan ◽  
Zhiping Yan ◽  
Ruixue Zhu ◽  
David Phillips
Keyword(s):  
Transient Absorption ◽  
Direct Detection ◽  
Reaction Pathway ◽  
Addition Reactions ◽  
Time Resolved ◽  
Nitrenium Ions ◽  
Future Studies ◽  
Nitrenium Ion ◽  
Design Time

Nitrenium ions are important reactive intermediates in chemistry and biology. In this work, femtosecond and nanosecond transient absorption (fs-TA and ns-TA) along with nanosecond time-resolved resonance Raman (ns-TR3) experiments were employed to examine the photochemical pathways of N-(4,4′-dibromodiphenylamino)-2,4,6-trimethylpyridinium BF4− (salt (DN) from just absorption of a photon of light to the production of the important N,N-di(4-bromophenyl)nitrenium ion 2. In acetonitrile (MeCN), the formation of halogenated diarylnitrenium ion 2 was observed within 4 ps, showing the vibrational spectra with strong intensity. The nucleophilic adduct reaction of ion 2 with H2O was also examined in aqueous solutions. The direct detection of the unique ortho adduct intermediate 3 shows that there is an efficient and exclusive reaction pathway for 2 with H2O. The results shown in this paper give new characterization of 2, which can be used to design time-resolved spectroscopy investigations of covalent addition reactions of nitrenium ions with other molecules in future studies.

Immobilization and Characterization of Tannase and its Haze-removing

2009 ◽  
Vol 15 (6) ◽  
pp. 545-552 ◽  
Author(s):  
Erzheng Su ◽  
Tao Xia ◽  
Liping Gao ◽  
Qianying Dai ◽  
Zhengzhu Zhang
Keyword(s):  
Kinetic Parameter ◽  
High Activity ◽  
Green Tea ◽  
Storage Stability ◽  
Residual Activity ◽  
Black Tea ◽  
Optimum Temperature ◽  
Oolong Tea ◽  
Optimum Ph

Tannase was effectively immobilized on alginate by the method of crosslinking-entrapment-crosslinking with a high activity recovery of 76.6%. The properties of immobilized tannase were investigated. Its optimum temperature was determined to be 35 ° C, decreasing 10 °C compared with that of free enzyme, whereas the optimum pH of 5.0 did not change. The thermal and pH stabilities of immobilized tannase increased to some degree. The kinetic parameter, Km, for immobilized tannase was estimated to be 11.6 × 10-4 mol/L. Fe2+ and Mn2+ could activate the activity of immobilized tannase. The immobilized tannase was also applied to treat the tea beverage to investigate its haze-removing effect. The content of non-estern catechins in green tea, black tea and oolong tea increased by 52.17%, 12.94% and 8.83%, respectively. The content of estern catechins in green tea, oolong tea and black tea decreased by 20.0%, 16.68% and 5.04%, respectively. The anti-sediment effect of green tea infusion treated with immobilized tannase was significantly increased. The storage stability and reusability of the immobilized tannase were improved greatly, with 72.5% activity retention after stored for 42 days and 86.9% residual activity after repeatedly used for 30 times.

scholarly journals Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

2021 ◽  
Vol 9 (6) ◽  
pp. 1331
Author(s):  
Arnaud Jéglot ◽  
Sebastian Reinhold Sørensen ◽  
Kirk M. Schnorr ◽  
Finn Plauborg ◽  
Lars Elsgaard
Keyword(s):  
Temperature Response ◽  
Microbial Transformation ◽  
Drainage Water ◽  
Microbial Populations ◽  
Agricultural Drainage ◽  
Bacterial Populations ◽  
Cold Temperatures ◽  
Cold Adapted ◽  
Agricultural Drainage Water ◽  
Temperature Responses

Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3−) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3− removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3− depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes’ composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3− removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

Tool Approach Directions for Machining Protrusion and Depression Features in an Object

1990 ◽  
Author(s):  
Chandrasekhar Karra ◽  
Thomas A. Phelps
Keyword(s):  
Product Development ◽  
Product Design ◽  
Tool Path ◽  
Low Cost ◽  
Production Costs ◽  
Competitive Market ◽  
Process Plans ◽  
Manufacturing Problems ◽  
Lower Production

Abstract The success of any industry in today’s highly competitive market is largely dependent on its ability to produce quality products, quickly and at low cost. Evaluating the effect of a product design on its manufacture is crucial in developing efficient designs. Any potential manufacturing problems detected at this stage can be corrected by modifying the design, leading to shorter product development cycles and lower production costs. This paper presents an algorithm to determine feasible tool approach directions. The algorithm is based on detecting if any part of the object obstructs the tool path. The basis for the algorithm is determining feasible approach directions and clearances around a planar polygonal face. The algorithm is applicable to both protrusions and depressions. The information is useful in performing manufacturability analysis of designs and develop process plans.

Economic performance of organic vs conventional vegetables production in Gaza strip

2014 ◽  
Vol 2 (2) ◽  
pp. 9-18
Author(s):  
Ahmed Abu Shaban
Keyword(s):  
Organic Farming ◽  
Gaza Strip ◽  
Farming System ◽  
Organic Production ◽  
Economic Feasibility ◽  
Production Costs ◽  
Economic Potential ◽  
Vegetable Crops ◽  
Gross Margin ◽  
Lower Production

Organic farming has achieved significant growth in developing countries. However, it is still in some areas such as Gaza strip at embryonic stage. Introduction and promotion of organic farming would need more information about economic feasibility of shifting from the existing conventional farms to organic farming system. This is the main aim of this study. Data was collected from 100 randomly selected farmers in southern area of Gaza strip using standard questionnaire. Additional focus group discussions were conducted for further qualitative analyses. Data was also collected from the organic farm of Safe Agriculture Association where vegetables are organically produced and marketed. Gross margin and comparative analyses were used to describe cost structure of conventional and organic production and to assess economic potentialities to shift to organic farming. Results varied among vegetable crops as some crops showed very high economic potential to shift to organic farming while other crops did not. Major reasons for crops with good potential were higher yield under organic farming, premium market prices and lower production costs. Major reasons for lower economic potential to shift were the significant lower yield and higher production costs. The study recommends further technical research to explore organic production techniques that allows for higher yield and lower production cost. The study also recommends further market research to investigate consumers' preferences and willingness to pay for organic products.

scholarly journals Simulation of Holstein and Jersey profitability by varying milk price payment system

2010 ◽  
Vol 39 (4) ◽  
pp. 913-923 ◽  
Author(s):  
Daniel de Noronha Figueiredo Vieira da Cunha ◽  
José Carlos Pereira ◽  
Oriel Fajardo de Campos ◽  
Sebastião Teixeira Gomes ◽  
José Luis Braga ◽  
...  
Keyword(s):  
Payment System ◽  
Production Costs ◽  
Farm Size ◽  
Protein Percentage ◽  
Bonus Payment ◽  
Holstein Breed ◽  
Percentage Unit ◽  
Economic Indexes ◽  
Lower Production

The objective of the study was to compare through simulation Holstein and Jersey breed herds, considering the farm size, zootechnical and economic indexes and profitability of the activity, by using different systems of bonus payment for milk price based in percentage of fat and protein in the milk. The comparisons were made, initially, disregarding the bonus payment for milk price based on the increase in the fat and protein percentage in milk. After that, the profitability of the activity was compared considering bonus payment for milk price of the Jersey breed herd, for each unit increment in fat and protein percentage in milk above those obtained with the Holstein breed herd. Finally, the profitability of the activity was compared by using the bonus payment for milk price based on quality of that used by company A systems. When there was no bonus payment for milk price based in the percentage of fat and protein in the milk, the profit obtained with the Holstein breed was higher because of the lower production costs. In order to make the activity more profitable with the Jersey breed, a bonus payment of 5% for milk price would be needed for each percentage unit of fat above the percentage of that obtained with the Holstein breed, or a bonus payment of 10% in the milk price for each percentage unit of protein above the percentage obtained with the Holstein breed. By using the bonus payment system proposed by Gimenes and Ponchio, the annual profit with the Jersey breed was higher than the profit obtained with the Holstein breed. However, with the application of the bonus payment system used by company A, the Holstein breed became more profitable than the Jersey breed.

Sign in / Sign up

Export Citation Format

Share Document