scholarly journals Study of the correlation between the clinical manifestations of the inflammatory periodontal diseases and the microbiome of periodontal pathogenic microflora in young representatives of a mixed population of the European region

2021 ◽  
Vol 18 (4) ◽  
pp. 433-444
Author(s):  
E. A. Tikhomirova ◽  
I. V. Zudina ◽  
E. S. Slazhneva ◽  
L. Yu. Orekhova ◽  
Yu. L. Denisova ◽  
...  
Keyword(s):  
Young People ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Plaque Formation ◽  
Periodontal Pocket ◽  
Purulent Discharge ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Dental Biofilm ◽  
Pd Complexes

The age of inflammatory periodontal disease (PD) manifestations has tended to decrease over the past decades. The study of the range of periodontal pathogens in young people and their influence on the PD manifestation contributes to the predictor identification for the early prevention of this pathology.The aim was to study the correlation between the range of periodontal pathogens in the dentoalveolar sulcus/periodontal pocket (DS/PC) contents and the clinical PD manifestations in young people.We examined 28 patients (23.1 ± 0.93 years) with dental biofilm-induced gingivitis (BG), 24 patients (30.7 ± 0.6 years) with aggressive periodontitis (AgP), and 87 clinically periodontally healthy patients (21.1 ± 0.49 years) (Control). The hygiene index and the periodontal status were determined in all patients. DNA of five periodontal pathogens was identified by PCR in the DS/PC contents. The statistical analysis was performed in Statistica 13.3. The critical significance level was p ≤ 0.05.DNA was not observed in 60.9 % of the control group samples and 7.1 % of the BG group samples. In other cases, the bacteria were found separately and as part of bacterial complexes. P.g. and T.f. were most often detected in all groups. P.g. (U = 474, р < 0.01) and A.a. (U = 209, р >< 0.05) significantly contributed to the plaque formation in the control group, T.d. – in BG and AgP groups (U = 37.5, р >< 0.05 and U = 34, р >< 0.05, respectively). In the AgP group, purulent discharge was more often recorded if T.d. was detected in the PC contents (χ2  = 5.53, р >< 0.05). T.f. + P.i. and P.g. + T.f. + P.i. complexes were exclusively associated with PD. Complexes of four bacteria were found only in the AgP group. The association of periodontal pathogens and their complexes with different PD forms was revealed.>< 0.01) and A.a. (U = 209, р <0.05) significantly contributed to the plaque formation in the control group, T.d. – in BG and AgP groups (U = 37.5, р <0.05 and U = 34, р <0.05, respectively). In the AgP group, purulent discharge was more often recorded if T.d. was detected in the PC contents (χ2  = 5.53, р <0.05). T.f. + P.i. and P.g. + T.f. + P.i. complexes were exclusively associated with PD. Complexes of four bacteria were found only in the AgP group.The association of periodontal pathogens and their complexes with different PD forms was revealed.

scholarly journals Understanding the microbial components of periodontal diseases and periodontal treatment-induced microbiological shifts

2020 ◽  
Author(s):  
Ioannis Fragkioudakis ◽  
Marcello P. Riggio ◽  
Danae Anastasia Apatzidou
Keyword(s):  
Periodontal Diseases ◽  
Bacterial Species ◽  
Oral Microbiome ◽  
Periodontal Pocket ◽  
Oral Microbiota ◽  
Periodontal Pathogens ◽  
Susceptible Host ◽  
Modern Culture ◽  
Microbial Identification ◽  
Dental Biofilm

In the mid-1960s the microbial aetiology of periodontal diseases was introduced based on classical experimental gingivitis studies . Since then, numerous studies have addressed the fundamental role that oral microbiota plays in the initiation and progression of periodontal diseases. Recent advances in laboratory identification techniques have contributed to a better understanding of the complexity of the oral microbiome in both health and disease. Modern culture-independent methods such as human oral microbial identification microarray and next-generation sequencing have been used to identify a wide variety of microbial taxa residing in the gingival sulcus and the periodontal pocket. The first theory of the ‘non-specific plaque’ hypothesis gave rise to the ‘ecological plaque’ hypothesis and more recently to the ‘polymicrobial synergy and dysbiosis hypothesis’. Periodontitis is now considered to be a multimicrobial inflammatory disease in which the various bacterial species within the dental biofilm are in a dysbiotic state and this imbalance favours the establishment of chronic inflammatory conditions and ultimately the destruction of tooth-supporting tissues. Apart from the known putative periodontal pathogens, the whole biofilm community is now considered to play a role in the establishment of inflammation and the initiation and progression of periodontitis in a susceptible host. Treatment is unlikely to eliminate putative pathogens but, when it is thoroughly performed it has the potential to establish a healthy ecosystem by altering the microbial community in numbers and composition and also contribute to the maturation of the host immune response.

Genomic composition of microbiol gingival sulcus and periodontal pockets in young persons

2020 ◽  
Vol 20 (1) ◽  
pp. 19-25
Author(s):  
S. L. S.L. Blashkova ◽  
T. N. Modina ◽  
A. K. Abdrakhmanov ◽  
D. A. Zinecker ◽  
E. V. Mamaeva ◽  
...  
Keyword(s):  
Risk Factors ◽  
Young People ◽  
16S Rrna ◽  
Periodontal Diseases ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Periodontal Pocket ◽  
Control Group ◽  
The Republic

Relevance. Risk factors of local importance plays a crucial role in the development of inflammatory periodontal diseases, but the profile of representation and the role of origin microbial markers continues to be refined, what explains the increasing interest by the metagenomic studies. Purpose. To compare the genomic composition of the microbiota of the periodontal sulcus and periodontal pocket by healthy patients with inflammatory periodontal diseases living on the territory of Kazan, the Republic of Tatarstan. Materials and methods. The study included 25 young people (11 boys, 14 girls) aged 18-19 years, with inflammatory periodontal diseases (chronic generalized catarrhal gingivitis (12 people), chronic generalized periodontitis of mild severity (13 people)). The control group consisted of 11 donors without inflammatory periodontal disease.Results. In the present study structures of microbial communities of periodontal spaces has been analyzed with using the sequencing of fragments of bacterial 16s rRNA genes (regions V3 and V4). Results of the analysis allowed to get a real idea of its composition and to determine both known and previously undefined uncultivated phylotypes. Conclusions. It was shown that in the group of the patients with inflammatory periodontal diseases there were identified 183 phylotypes at the level of genus (Mogibacteriacea, TM7 3, Rs–045, Dethiosulfovibrionaceae) relating to 17 phyls (phylum), that is a synonym of type in taxonomy (taxon between Kingdom and class). By the patients with chronic generalized periodontitis of mild severity, it was not possible to reliably isolate the phylotypes present in increased amounts in relation to chronic catarrhal gingivitis; in relation to control – there was a statistically significant increase in the proportion of families Porphyromonadaceae, Peptostreptococcaceae and the proportion of genera Dialister, Filifactor, Parvimonas, Tannerella, Treponema. 

Comparative analysis of polymorphic variants of IL-17A and MMP-1 genes with the risk of developing chronic periodontitis in petrochemical workers

2020 ◽  
Vol 60 (10) ◽  
pp. 687-693
Author(s):  
Iskander I. Zaidullin ◽  
Denis O. Karimov ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Control Group ◽  
Periodontal Tissues ◽  
Dental Examination ◽  
Number Of Patients ◽  
Increased Risk ◽  
Harmful Substances

The susceptibility to the development and progression of inflammatory periodontal diseases, which depends on genetic and external factors (smoking, stress, oral hygiene), varies widely. In the development of these diseases, an important role is played not only by the presence of periodontal pathogenic microorganisms, but also by the presence of congenital or acquired immunodeficiency, immunoregulatory defects. The immune system plays a key role in the physiological and pathological processes of periodontal tissues. In this regard, IL17, produced by CD4+ Th cells, which has both Pro-inflammatory and protective activity, is of particular interest in the pathogenesis of periodontitis. The aim of study was to identify the relationship between polymorphic loci of the IL-17A (rs2275913) and MMP-1 (rs1799750) genes and clinical manifestations of chronic periodontitis in petrochemical workers. Dental examination was performed in 92 ethylene oxide production workers with chronic periodontitis and 74 patients with chronic periodontitis who did not come into contact with chemical factors (control group). Genotyping of polymorphisms rs2275913 of the IL17A gene and rs1799750 of the MMP1 gene was performed by allele-specific real-time polymerase chain reaction (PCR). Hygienic assessment of the degree of air pollution of the working area with harmful substances was carried out by gas chromatography according to the guidelines for the determination of harmful substances in the air № 5098-89, № 3119-84. When comparing the results of studies of both groups, there were no statistically significant differences in the frequency distributions of allelic variants and genotypes of the IL-17A and MMP-1 genes. The AA/AG genotypes of the IL-17A gene were associated with an increased risk of severe disease compared to the GG genotype in workers in the main group (OR=6.1; 95% CI 1.33-28.5; p=0.021) and in the control group (OR=7.26; 95% CI 1.34-39.25; p=0.016). Carriers of the A allele in the control group increased the risk of severe chronic periodontitis by 2.4 times compared to carriers of the G allele (OR=2.41; 95% CI 1.19-4.87; p=0.014). During the dental examination of employees of the ethylene oxide plant, the clinical course of periodontal diseases was more severe in comparison with the control group, and the number of patients with severe periodontitis was twice as high. It was found that the AA/AG genotypes of the IL-17A gene and the carrier of the A allele are associated with increased susceptibility to the development of severe chronic periodontitis. The association between the MMP-1 gene polymorphism and the risk of severe forms of chronic periodontitis has not been established. A risk factor for the development of inflammatory periodontal diseases in employees of the petrochemical complex is a complex of harmful production factors.

scholarly journals Вплив поліморфізму генів PTHR1 та COL1A1 на розвиток захворювань тканин пародонта у людей молодого віку

2017 ◽  
Author(s):  
M. A. Luchynskyi ◽  
Y. V. Boliuk ◽  
V. M. Luchynskyi
Keyword(s):  
Young People ◽  
Periodontal Diseases ◽  
Main Group ◽  
Control Group ◽  
Type I ◽  
Periodontal Tissue ◽  
Endogenous Factors ◽  
Stage Of Development ◽  
The Difference ◽  
Collagen Chain

At the present stage of development of dentistry, the leading Ukrainian and foreign scientists devote a considerable part of the research to a deeper study of the etiology and pathogenetic mechanisms of periodontal tissue diseases and the influence of various exogenous and endogenous factors on their course.The aim of the study – to learn the ability and methods of forecasting and early diagnosis of the periodontal tissue lesions in young people. Materials and Methods. During our research we examined 24 young people with periodontal tissue diseases, who were included to the main group, and 15 healthy people, who formed the control group. The complex clinical examination was performed in each research group. It was studied the distribution of polymorphous variants of the type I parathormone receptor and the α1-chain of collagen gene with a help of polymerase chain reaction by restrictase cleavage of DNA fragments and electrophoresis in polyacrylamide gel (AA/BA 29:1). Results and Discussion. The distribution of genotypes by PTHR1 gene in control group was similar to those in main group (p>0.05). Also we didn’t find the difference between frequencies of the separate alleles in people with periodontal tissue pathology and without it (p>0.05). Yes, the repetitions of the allele 5 encoding normal type I parathormone receptor were found more often, comparing with the allele 6 that is responsible for the formation of unfunctional PTHR1 (р<0.001) in both main and control groups. The dominance of the genotype TT, which corresponds to the pathology, was found in young people with the periodontal tissue lesions – (38.46 ± 4.79) %, while among the control group the genotype of norm GG was met the most often – (68.24±5.08) %. Also, the frequency of repetitions of the allele T encoding the imperfect collagen chain was (57.60±3.79) % in young people with periodontal diseases, and in the control group this figure was (13.27±2.81) %, p<0.001.  Conclusions. According to our results the presence of allele T and genotype TT that correspond the imperfect collagen chain may be one of the causes of periodontal tissue pathology.

scholarly journals Periodontal Pathogens Invade Gingiva and Aortic Adventitia and Elicit Inflammasome Activation in αvβ6 Integrin-Deficient Mice

2015 ◽  
Vol 83 (12) ◽  
pp. 4582-4593 ◽  
Author(s):  
Irina M. Velsko ◽  
Sasanka S. Chukkapalli ◽  
Mercedes F. Rivera-Kweh ◽  
Donghang Zheng ◽  
Ikramuddin Aukhil ◽  
...  
Keyword(s):  
Atherosclerotic Plaque ◽  
Inflammatory Responses ◽  
Periodontal Diseases ◽  
Periodontal Pocket ◽  
Bacterial Invasion ◽  
Inflammasome Activation ◽  
Periodontal Pathogens ◽  
Content Type ◽  
Amyloid A ◽  
Significant Difference

The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of the integrin β6−/−mouse model to study the causality. We investigated the ability of a polymicrobial consortium ofPorphyromonas gingivalis,Treponema denticola,Tannerella forsythia, andFusobacterium nucleatumto colonize the periodontium and induce local and systemic inflammatory responses. Polymicrobially infectedItgβ6−/−mice demonstrate greater susceptibility to gingival colonization/infection, with severe gingival inflammation, apical migration of the junctional epithelium, periodontal pocket formation, alveolar bone resorption, osteoclast activation, bacterial invasion of the gingiva, a greater propensity for the bacteria to disseminate hematogenously, and a strong splenic T cell cytokine response. Levels of atherosclerosis risk factors, including serum nitric oxide, oxidized low-density lipoprotein, serum amyloid A, and lipid peroxidation, were significantly altered by polybacterial infection, demonstrating an enhanced potential for atherosclerotic plaque progression. Aortic gene expression revealed significant alterations in specific Toll-like receptor (TLR) and nucleotide-binding domain- and leucine-rich-repeat-containing receptor (NLR) pathway genes in response to periodontal bacterial infection. Histomorphometry of the aorta demonstrated larger atherosclerotic plaques inItgβ6−/−mice than in wild-type (WT) mice but no significant difference in atherosclerotic plaque size between mice with polybacterial infection and mice with sham infection. Fluorescencein situhybridization demonstrated active invasion of the aortic adventitial layer byP. gingivalis. Our observations suggest that polybacterial infection elicits distinct aortic TLR and inflammasome signaling and significantly increases local aortic oxidative stress. These results are the first to demonstrate the mechanism of the host aortic inflammatory response induced by polymicrobial infection with well-characterized periodontal pathogens.

scholarly journals METHOD FOR TREATING CHRONIC GENERALIZED PERIODONTITIS OF LIGHT AND MEDIUM GRAPES

2020 ◽  
Vol 16 (2) ◽  
pp. 53-58
Author(s):  
Karen Karakov ◽  
Emilia Khachaturyan ◽  
Marat Uzdenov ◽  
Laura Uzdenoba ◽  
Nelli Vanchenko ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Treatment Regimen ◽  
Treatment Time ◽  
Periodontal Diseases ◽  
Periodontal Pocket ◽  
Control Group ◽  
Moderate Severity ◽  
Affected Area ◽  
Periodontal Tissues ◽  
Complex Therapy

Subject. The article presents the data of a comparative analysis of the traditional method of treating chronic generalized periodontitis of mild to moderate severity and with the use of periodontal dressings in combination with an antibacterial photodynamic system. The goal is to study the effectiveness of the methodology of complex therapy for the treatment of periodontal diseases using periodontal dressings and antibacterial photodynamic therapy. Methodology. We observed 120 patients with inflammatory periodontal diseases aged 18 to 55 years (men ― 52, women ― 68), who were divided into the control and main groups. In the control group, treatment was carried out according to the traditional scheme of drug therapy, in the main ― according to the method of complex therapy. The treatment regimen according to this technique included the use of periodontal dressings and an antibacterial photodynamic therapy system: photosynthesis was applied to the affected area of the periodontal pocket, after which the pathogenic microorganisms were stained for 1-3 minutes, then the liquid was thoroughly washed from the periodontal pockets and the laser affected the affected area for 60 seconds. Results. The proposed treatment regimen using an antibacterial photodynamic system in combination with periodontal dressings allows to achieve a pronounced and prolonged anti-inflammatory effect in periodontal tissues without local and systemic use of antibiotics, reduces treatment time for mild periodontitis by 29, average ― by 57 %, reduces the likelihood recurrence, lengthens the duration of remission. Findings. The use of complex treatment of periodontitis of mild to moderate severity allows the method to accelerate the restoration of periodontal tissues, relieve inflammation, saturate the tissues with oxygen, reduce the rate of relapse by 2.6 times and reduce the duration of relapse of this disease within 24 months. Our data allow us to implement this treatment regimen in practical healthcare.

scholarly journals Specific characteristics of rapid diagnosis in periodontology

2021 ◽  
Vol 18 (2) ◽  
pp. 196-203
Author(s):  
S. P. Rubnikovich ◽  
L. N. Dedova ◽  
P. A. Semizhon ◽  
Yu. L. Denisova ◽  
O. V. Kandrukevich
Keyword(s):  
Periodontal Diseases ◽  
Genetic Material ◽  
Clinical Signs ◽  
Rapid Test ◽  
Laboratory Diagnosis ◽  
Periodontal Pocket ◽  
Control Group ◽  
Treponema Denticola ◽  
Microbiological Diagnosis ◽  
Laboratory Measures

Currently, the advanced studies have been justified and implemented in the Republic of Belarus that enable the production and a practical use of a domestic rapid test and allow dentists to apply microbiological diagnosis methods.The objective of the study was to develop a domestic rapid test for periodontology, indicating the procedure for preparatory clinical and laboratory measures using PCR and trypsin-like activity of periopathogenic microflora (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus)Dental examination was performed in 60 patients with clinical signs of generalized periodontitis and a 5‒6 mm deep pathological dentogingival pocket (PDGP), aged 35–44 (the main group), and 140 patients without signs of periodontal diseases, aged 20–24 (the control group). These patients were selected among the visitors of Chair No. 3 of the Belarusian State Medical University. The laboratory diagnosis was made using PCR and evaluating the trypsin-like activity of periopathogenic microflora.The algorithm of preparatory clinical and laboratory measures was suggested for diagnosis of periodontal diseases, including material sampling and identification of periopathogenic microorganisms in the sample using a biochemical method; sampling, preservation and storage of material for quantitative PRC testing; and the identification of the genetic material of periopathogenic microorganisms by means of a real-time quantitative PRC method.The basic principles of microbiological diagnosis in periodontology enable making a true diagnosis, planning treatment and assessing the treatment results.We examined the content of the gingival sulcus and/or the periodontal pocket in patients who have periodontal diseases. It was noted that the most rational tools for removing the periodontal pocket content in patients with periodontal diseases was a paper pin.The development of a domestic express test in periodontology demonstrated the need of preparatory clinical and laboratory activities using PCR and trypsin-like activity of periopathogenic microflora (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus).

scholarly journals Safety and Preliminary Efficacy of a Novel Host-Modulatory Therapy for Reducing Gingival Inflammation

2021 ◽  
Vol 12 ◽  
Author(s):  
Hatice Hasturk ◽  
Fabian Schulte ◽  
Melissa Martins ◽  
Homa Sherzai ◽  
Constantinos Floros ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Inflammatory Diseases ◽  
Periodontal Diseases ◽  
Periodontal Pocket ◽  
Lipid Mediator ◽  
Control Group ◽  
Gingival Inflammation ◽  
Lipoxin A4 ◽  
Resolution Of Inflammation ◽  
Oral Rinse

BackgroundPeriodontal disease is among the sixth most common inflammatory diseases worldwide with high risk to promote complications from other inflammatory diseases including diabetes, cardiovascular disease and Alzheimer’s Disease. Failure of active resolution of inflammation pathways is implicated in pathogenesis of periodontal diseases, including gingivitis. Lipoxin A4 (LXA4), a member of the specialized pro-resolving lipid mediators (SPMs) that drive resolution of inflammation via GPC-receptor mediated pathways, offered therapeutic advantages in preclinical models of periodontitis.MethodsWe conducted a randomized, placebo-controlled, parallel-group Phase 1 clinical trial to determine the safety and preliminary efficacy of an LXA4 analog in patients with gingival inflammation. One hundred twenty-seven (127) individuals were randomized to daily use of an oral rinse containing a LXA4 mimetic, methyl ester-benzo-lipoxin A4 (BLXA4), placebo rinse or a no-rinse control group for 28 days. Treatment emergent adverse events (TEAEs) were assessed for safety, the primary outcome. Secondary outcomes included the change in the level of gingival inflammation and periodontal pocket depth (PD). Serum SPMs were monitored using targeted lipid mediator lipidomics to assess potential systemic impact of BLXA4.ResultsThe frequency of TEAEs was similar in BLXA4 and placebo-treated groups with no study-related SAEs. Once-daily rinsing with BLXA4 for 28-days resulted in a greater decrease in gingival inflammation compared to placebo rinse and no-rinse control groups (mean change: 0.26 GI unit vs 0.21 and 0.17, respectively). PD reduction was also greater with BLXA4 oral rinse compared to placebo and no-rinse groups (mean reduction: 1.23 mm vs. 0.71 mm and 0.46 mm, respectively). Topical application of BLXA4 increased serum levels of SPMs.ConclusionTreatment with BLXA4 reduces local inflammation, and increases abundance of pro-resolution molecules systemically, which may dampen inflammation that can mediate progression and course of inflammatory diseases beyond periodontitis.Clinical Trial RegistrationClinicalTrials.gov, identifier (NCT02342691).

Role of antimicrobial peptides system in inflammatory periodontal diseases non-specific oral cavity protection

2021 ◽  
Vol 66 (7) ◽  
pp. 422-427
Author(s):  
N. V. Davidovich ◽  
N. V. Solovieva ◽  
A. S. Galieva ◽  
S. Yu. Lepeshkin ◽  
E. N. Bashilova ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Antimicrobial Peptides ◽  
Chronic Periodontitis ◽  
Pathogenic Bacteria ◽  
Periodontal Diseases ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Local Immunity ◽  
Microbial Invasion

The system of antimicrobial peptides (AMP) is one of the most ancient mechanisms of the macroorganism resistance to infectious pathogens invasion. The aim of the study was to determine the role of the antimicrobial peptides system and periodontal pathogenic markers in the development and progression of inflammatory periodontal diseases. Gingival pocket washes (91 samples in total) for the research were received from patients with inflammatory periodontal diseases (chronic periodontitis and gingivitis) and intact periodontium. Using ELISA, the content of antimicrobial peptides was determined: human alpha-defensin (HNP 1-3), beta-defensin (HBD 1-3) and cathelicidin (LL-37). Periodontal pathogenic markers were isolated during RT-PCR. The study revealed differences in AMP concentrations by groups: level of HBD 2 in patients with chronic periodontitis was 1,36 times higher than those in the group of patients with chronic gingivitis (p=0,023) and 2,39 times higher than those in the control group (p<0,001), the content of HNP 1-3 in the group of patients with chronic periodontitis was reduced by 1,23 times compared with the indicators of the group of patients with gingivitis (p=0,045) and by 1,97 times compared with the indicators of the control group (p<0,001). The frequency of detection of periodontal pathogenic bacteria genes was 88,0% in patients with periodontitis, 76,92% in patients with gingivitis and 33,3% in the group with intact periodontium. HBD 2 content moderately correlated with the definition of P. gingivalis (r=0,612; p=0,022), T. forsythensis (r= 0,434; p=0,015), A. actinomycetemcomitans (r=0,483; p=0,006), a moderate negative correlation was detected between the content of HNP 1-3 and the release of periodontal pathogens in associations (P. gingivalis with T. forsythensis and T. denticola) (r=-0,388; p=0,031) in the group of patients with chronic periodontitis. Thus, the revealed relationships and correlations indicate shifts in the processes of reparative regeneration of the oral cavity and the regulation of local immunity in response to microbial invasion.

scholarly journals Early Clinical Effectiveness of ER:Yag Laser in Association with the Red Complex of Bacteria in the Initial Treatment of Moderate Chronic Periodontitis

2014 ◽  
Vol 41 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Bl. Yaneva ◽  
E. Firkova ◽  
E. Karaslavova
Keyword(s):  
Chronic Periodontitis ◽  
Er:Yag Laser ◽  
Test Group ◽  
Clinical Effectiveness ◽  
Periodontal Therapy ◽  
Periodontal Pocket ◽  
Control Group ◽  
Microbiological Analysis ◽  
Periodontal Pathogens ◽  
Pocket Depth

Summary Chronic periodontitis is a result of polymicrobial infection and its treatment aims removal of dental calculus and biofi lm from the periodontal pocket. Many hand and power-driven instruments are created for this purpose. In recent years, lasers are widely discussed tools for periodontal therapy. The aim of the present study is to evaluate the early microbiological and clinical effectiveness of the Er:YAG laser in the treatment of chronic periodontitis. 30 patients with moderate chronic periodontitis were enrolled in the study. Using a split-mouth design they were treated either with Er:YAG laser (chisel tip, 100 mJ, 15 Hz, 5-6 water spray)-test group or with Gracey curettes- control group. Probing pocket depth (PPD), gingival recession (GR), clinical attachment level (CAL), bleeding on probing and plaque presence were evaluated at baseline and one month after the therapy. Microbiological samples were taken from the deepest four pockets of each quadrant from 20 randomly selected patients (n = 80). They were evaluated using real time PCR for periodontal pathogens from the red complex (Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia). One month after therapy in the control group PPD decreased from 4.59 mm to 3.36 mm, the CAL gain was 1.09 mm and the reduction of bleeding and plaque - from 87.9% to 33.7% and from 75.9% to 40.9%, respectively. In the test group PPD decreased from 4.58 mm to 3.15 mm, the CAL gain was 1.37mm and the reduction of bleeding and plaque was from 85.6% to 25.9% one month after the treatment. The results were statistically significant in favour of the laser group (p < 0.05). Microbiological analysis revealed that the pathogens from the red complex decreased 3 times in the control and 6 times in the test group. Therefore, the Er:YAG laser demonstrate pronounced early effectiveness in chronic periodontitis treatment and would be appropriate alternative of the conventional periodontal therapy.

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