scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

scholarly journals The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

2017 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Southern China ◽  
Pathogenic Fungus ◽  
Lethal Effect ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Rdna Its ◽  
Major Pest

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.

scholarly journals The isolation and identification of pathogenic fungi from Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3888 ◽  
Author(s):  
Xiang Meng ◽  
Junjie Hu ◽  
Gecheng Ouyang
Keyword(s):  
Entomopathogenic Fungi ◽  
Southern China ◽  
Biological Control Agent ◽  
Pathogenic Fungi ◽  
Control Agent ◽  
Morphological Identification ◽  
Pest Species ◽  
Stink Bug ◽  
Isolation And Identification ◽  
Highly Virulent

Background Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae), is one of the most widespread and destructive pest species on Litchi chinensis Sonn and Dimocarpus longan Lour in Southern China. Inappropriate use of chemical pesticides has resulted in serious environmental problems and food pollution. Generating an improved Integrated Pest Management (IPM) strategy for litchi stink-bug in orchard farming requires development of an effective biological control agent. Entomopathogenic fungi are regarded as a vital ecological factor in the suppression of pest populations under field conditions. With few effective fungi and pathogenic strains available to control litchi stink-bug, exploration of natural resources for promising entomopathogenic fungi is warranted. Methods & Results In this study, two pathogenic fungi were isolated from cadavers of adult T. papillosa. They were identified as Paecilomyces lilacinus and Beauveria bassiana by morphological identification and rDNA-ITS homogeneous analysis. Infection of T. papillosa with B. bassiana and P. lilacinus occurred initially from the antennae, metameres, and inter-segmental membranes. Biological tests showed that the two entomopathogenic fungi induced high mortality in 2nd and 5th instar nymphs of T. papillosa. B. bassiana was highly virulent on 2nd instar nymphs of T. papillosa, with values for cadaver rate, LC50 and LT50 of 88.89%, 1.92 × 107 conidia/mL and 4.34 days respectively. Discussion This study provides two valuable entomopathogenic fungi from T. papillosa. This finding suggests that the highly virulent P. lilacinus and B. bassiana play an important role in the biocontrol of T. papillosa in China. These pathogenic fungi had no pollution or residue risk, and could provide an alternative option for IPM of litchi stink-bug.

scholarly journals Cloning of Beauveria bassiana Chitinase Gene Bbchit1 and Its Application To Improve Fungal Strain Virulence

2005 ◽  
Vol 71 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Weiguo Fang ◽  
Bo Leng ◽  
Yuehua Xiao ◽  
Kai Jin ◽  
Jincheng Ma ◽  
...  
Keyword(s):  
Amino Acid ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Pathogenic Fungus ◽  
Amino Acid Level ◽  
Single Copy ◽  
Chitinase Gene ◽  
Streptomyces Avermitilis ◽  
Regulatory Sequence ◽  
Significant Similarity

ABSTRACT Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid cultures of Beauveria bassiana grown in a medium containing colloidal chitin. Bbchit1 had a molecular mass of about 33 kDa and pI of 5.4. Based on the N-terminal amino acid sequence, the chitinase gene, Bbchit1, and its upstream regulatory sequence were cloned. Bbchit1 was intronless, and there was a single copy in B. bassiana. Its regulatory sequence contained putative CreA/Crel carbon catabolic repressor binding domains, which was consistent with glucose suppression of Bbchit1. At the amino acid level, Bbchit1 showed significant similarity to a Streptomyces avermitilis putative endochitinase, a Streptomyces coelicolor putative chitinase, and Trichoderma harzianum endochitinase Chit36Y. However, Bbchit1 had very low levels of identity to other chitinase genes previously isolated from entomopathogenic fungi, indicating that Bbchit1 was a novel chitinase gene from an insect-pathogenic fungus. A gpd-Bbchit1 construct, in which Bbchit1 was driven by the Aspergiullus nidulans constitutive promoter, was transformed into the genome of B. bassiana, and three transformants that overproduced Bbchit1 were obtained. Insect bioassays revealed that overproduction of Bbchit1 enhanced the virulence of B. bassiana for aphids, as indicated by significantly lower 50% lethal concentrations and 50% lethal times of the transformants compared to the values for the wild-type strain.

scholarly journals JAMUR ENTOMOPATOGEN ASAL TANAH LEBAK DI SUMATERA SELATAN DAN POTENSINYA SEBAGAI AGENSIA HAYATI WALANG SANGIT (LEPTOCORISA ORATORIUS (F.))

2010 ◽  
Vol 10 (2) ◽  
pp. 154-161
Author(s):  
Effendy TA ◽  
Robby Septiadi ◽  
Abdullah Salim ◽  
Abdul Mazid
Keyword(s):  
Beauveria Bassiana ◽  
Analysis Of Variance ◽  
Entomopathogenic Fungi ◽  
Probit Analysis ◽  
Biological Agents ◽  
Biocontrol Agents ◽  
Stink Bug ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Leptocorisa Oratorius

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F).  Soil used for trapping entomopatogenic fungi, was taken from five locations  in South Sumatera.  Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design.  LT50­ of nymph mortality was determined with Probit Analysis.  Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp.  Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates.  The viability of B. bassiana conidia did not decrease during sub-culturing.  Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50­  of the stink bug.

scholarly journals Greenhouse evaluation of Beauveria bassiana (Balsamo) Vuillemin against Aphis craccivora (Das) on Fenugreek

2014 ◽  
Vol 6 (2) ◽  
pp. 852-856 ◽  
Author(s):  
K. Selvaraj ◽  
H. D. Kaushik
Keyword(s):  
Beauveria Bassiana ◽  
Sustainable Management ◽  
Biocontrol Agent ◽  
Pathogenic Fungus ◽  
Aphis Craccivora ◽  
Lc50 Value ◽  
Greenhouse Evaluation ◽  
Potential Biocontrol Agent ◽  
Field Efficacy ◽  
Lethal Time

The bioassay studies were carried out to determine the LC50 and LT50 of Beauveria bassiana against Aphis craccivora on fenugreek under greenhouse conditions. The results revealed that, the cumulative corrected mortality (CCM) was 43.50% at higher concentration (1×1010 spores/ml) and it was 20.85% at lowest concentration (1×104 spores/ml) at one day after treatment (DAT). The CCM decreased with decreasing conidial spore concentration. Likewise, at 2, 3, 4, 5, 6 and 7 DAT, almost same trend was observed. At 7 DAT, the CCM was 85.04% and 55.21% at 1×1010 spores/ml and 1×104 spores/ml, respectively. The LC50 value of B. bassiana against A. craccivora was 1.2×108 spores/ml. Mean lethal time (LT50) values were worked out 73, 89, 97, 112, 126, 138 and 157 hours for 1010, 109, 108, 107, 106, 105 and 104 spores/ml, respectively. By testing the field efficacy of B. bassiana against A. craccivora, this insect pathogenic fungus can be used as potential biocontrol agent for the sustainable management of aphid in fenugreek crop.

scholarly journals Effectiveness of Cordyceps sp. and Beauveria Bassiana Against Large Cabbage-heart Caterpillar, Crocidolomia Pavonana Fabricius (Lepidoptera: Crambidae)

2022 ◽  
Vol 16 ◽  
pp. 14-18
Author(s):  
Agus Dana Permana ◽  
Sally Marlinda ◽  
Agus Susanto ◽  
Amin Setyo Leksono
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insect Pest ◽  
Probit Analysis ◽  
Efficacy Trial ◽  
Natural Area ◽  
Cooking Oil ◽  
Field Efficacy ◽  
Major Pest ◽  
Screen House

Crocidolomia pavonana Fabr. (Lepidoptera: Crambidae) is a major pest on various cabbage crops in Indonesia. Various insecticides have been used to control this insect pest including several entomopathogenic fungi, and this cabbage caterpillar has become resistant to various insecticides. In order to develop insecticides from entomopathogenic fungi, species or varieties from natural area such as forest are needed. A bioassays of Cordyceps sp. and Beauveria bassiana has been carried out in the laboratory. Inoculum of Cordyceps sp. obtained from the Dipterocarpaceae forest in South Kalimantan, Indonesia recovered from dead ants by the mycelium of Cordyceps sp. Four concentration of Cordyceps sp. and Beauveria bassiana inoculum and tween80 as a control were tested against 3rd instar larvae of C. pavonana with five replications. Field efficacy trial was carried out in a screen house (8 x 12 M2) using Chinese cabbage plants (Brassica juncea) aged 28 days after sowing, with a spacing of 0.4 M between rows. Three formulation of Cordyceps sp. and tween80 as a control were used with 6 replications. The result of the bioassays in the laboratory showed that Cordyceps sp. with concentration of 1 x 107 conidia mL-1 was able to give 100% mortality od C. pavonana larvae, while B. bassiana with the same concentration caused morality of C. pavonana larvae of 98.33%, although statistically not significantly different. The results of probit analysis also showed that LC90 Cordyceps sp. against cabbage caterpillar larvae was 1.57 x 106 conidia mL-1, while B. bassiana required a concentration of 2.6 x 106 conidia mL-1 to reach the LC90 value. The results of filed trial showed that the formulation of Cordyceps sp. 1 x 107 conidia mL-1 using palm oil cooking oil was able to give an average mortality of 86.7%, and significantly higher (P<0.05) than the other treatment. The results of this study indicate that the inoculum of Cordyceps sp. from Dipterocarpaceae forest is very promising to be developed as an insecticide to control C. pavonana in cabbage

scholarly journals PENGGUNAAN CENDAWAN ENTOMOPATOGEN Beauveria bassiana (BALSAMO) VUILLEMIN DAN Lecanicillium lecanii (ZIMM) ZARE & GAMS UNTUK MENGENDALIKAN Helopeltis antonii SIGN (HEMIPTERA: MIRIDAE) The Use of Entomopathogenic Fungi Beauveria bassiana (Balsamo)Vuillemin

2018 ◽  
Vol 8 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Sri Hastuti Anggarawati ◽  
Teguh Santoso ◽  
Ruly Anwar
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Low Cost ◽  
Instar Nymph ◽  
Pathology Laboratory ◽  
Tea Plantation ◽  
Lecanicillium Lecanii ◽  
Lc50 Value ◽  
Insect Pathology

Helopeltis sp. has been known as one of major pests on tea, cacao and cashew plantation. Recently, genus Helopeltis is also reported attack Acacia plantation in Sumatra and Kalimantan. For such extensive plantation, low cost biocontrol agents like entomopathogenic fungi are choosen because of the simplicity for mass production. The objective of this study was to measure the effectiveness of Beauveria bassiana and Lecanicillium lecanii against Helopeltis sp. Both fungi that were used in this study were obtained from IPB Insect Pathology Laboratory. The tested insect, Helopeltis sp. was collected from Gunung Mas tea plantation. The insects were reared in laboratory. Four level of conidial density, 109, 108, 107, 106 conidia/ mL were applied to Helopeltis adult (B. bassiana) and 3rdinstar nymph (L. lecanii). Daily mortality was observed until seven days post treatment. The result showed that L. lecanii at 106 conidia/mL caused 96.25% mortality of 3rd instar nymph of Helopeltis sp. with the LC50 value at two days observation was 1.03 x 106 conidia/ mL, LT50 was 1.198 days and LT95 was 5.25 days. On the other hand, B. bassiana at 106 conidia/ mL caused 81.25%. mortality of adult Helopeltis sp. while 100% mortality could be attained by using 108 conidia/ mL. The LC50 of B. bassiana was 3.2 x 104 conidia/ mL, at four days observation and LT50 was 4.214 days. The two fungi were thus judged effective against Helopeltis sp. in this bio assay.Key words: Beauveria bassiana, conidia, density, Helopeltis sp., Lecanicillium lecanii, mortality.

scholarly journals PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÅL)

2018 ◽  
Vol 11 (2) ◽  
pp. 122-132
Author(s):  
Yadi Suryadi ◽  
Wartono Wartono ◽  
Dwi Ningsih Susilowati ◽  
Puji Lestari ◽  
Cyntia Nirmalasari ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Internal Transcribed Spacer ◽  
Insect Pests ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Its Rdna ◽  
Stink Bug ◽  
West Java ◽  
Nilaparvata Lugens Stål

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2  and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.

scholarly journals Entomopathogenic Fungal Screening against Two Spotted Spider Mites, Tetranychus urticae Koch in Tomato and Broad Mite, Polyphagotarsonemus latus (Banks) in Chilli

2021 ◽  
Author(s):  
S. Sumaiya Parveen ◽  
K. Ramaraju ◽  
S. Jeyarani
Keyword(s):  
Tetranychus Urticae ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Spider Mites ◽  
Natural Occurrence ◽  
Polyphagotarsonemus Latus ◽  
Lc50 Value ◽  
Fungal Entomopathogens ◽  
Broad Mite ◽  
Tetranychus Urticae Koch

Background: Entomopathogenic fungi are microorganisms that specifically infect and often kill insects and other arthropods. Most are non-pathogenic to plants and relatively non-toxic to humans and animals. Use of fungal entomopathogens can be an alternative to insecticides and could help in bio-intensive pest management. The current study aimed to study the pathogenicity of entomopathogenic fungal isolates against two Spotted Spider Mites, Tetranychus urticae Koch in tomato and broad Mite, Polyphagotarsonemus latus (Banks) in chilli. Methods: An extensive survey for the natural occurrence of entomopathogenic fungi on insects and mites was made in different agro climatic regions of Tamil Nadu during 2013 and 2014. Survey revealed the occurrence of two entomopathogenic fungi viz., Beauveria bassiana (Balsamo) Vuillemin and Metarhizium flavoviride Gams and Rozsypal var. minus. Laboratory experiment was conducted to evaluate the pathogenicity of fourteen isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii against Tetranychus urticae and Polyphagotarsonemus latus. Result: In opposition to T. urticae, the Beauveria bassiana isolate Bb 112 showed the highest toxicity of all the other isolates tested, with low LC50 value of 0.6 x106 spores ml-1 and LT50 of 92.60h. Against P. latus, Bb 112 had high virulence with low LC50 value of 0.3x106 spores ml-1 and LT50 value of 92.32h. Hence, there is potential for use of entomopathogenic fungi in the field conditions as an alternate control method in combating the insect pests and other arthropod pests since they are considered natural mortality agents and are environmentally safe.

Sign in / Sign up

Export Citation Format

Share Document