NEONATAL SEPSIS;

Objectives: To determine the diagnostic accuracy of C-reactive protein in the diagnosis of neonatal sepsis keeping blood cultures as gold standard. Study Design: Descriptive cross-sectional study. Setting: Pediatric Unit of Lady Reading Hospital Peshawar Pakistan. Duration: Six months from 09-06-2012 to 08-12-2012. Methodology: Total of 196 patients meeting the required inclusion criteria with clinical suspicion of sepsis. Those neonates were subjected to investigations. C.R.P. was tested using the Quantitative method according to the instructions provided with the kit. By keeping blood culture as gold standard, patients with both positive and negative cultures were taken and the results compared to the results of C.R.P. in these subjects being positive or negative. Results: Among the 196, majority of the neonates included were less than a week old having a mean age of 4.5 days. There were 57 (29%) females and 139 (71%) males, with male to female ratio of 2.4:1. Blood cultures were positive in 85 (43%) and negative in 111 (57%) cases, while C.R.P. was positive in 95 (48%) and negative in 101 (52%) cases. Sensitivity, specificity, and positive and predictive values of C-reactive protein were calculated using formulas, and they turned out to be 77.6%, 73.8%, 69.4%, and 81.2% respectively with accuracy being 0.41%. Conclusion: An accurate and timely diagnosis of early onset neonatal sepsis remains challenging to the clinician as well as laboratory. Physicians can prevent unnecessary antibiotic use by performing the qualitative estimation CRP as a single, rapid and inexpensive test with a negative predictive value of 81.2%.

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MATERNAL C-REACTIVE PROTEIN IN PREGNANCY AND ITS RELATION WITH EARLY ONSET NEONATAL SEPSIS

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v71i4.3537 ◽

2021 ◽

Vol 71 (4) ◽

pp. 1130-33

Author(s):

Muhammad Zahid ◽

Saeed Zaman ◽

Sohail Shahzad ◽

Sajid Ali Shah ◽

Sana Javed ◽

...

Keyword(s):

Neonatal Sepsis ◽

Early Onset ◽

Signs And Symptoms ◽

Cross Sectional Study ◽

Military Hospital ◽

P Value ◽

C Reactive Protein ◽

Cross Sectional ◽

Reactive Protein ◽

Increased Risk

Objective:To determine association of early-onset neonatal sepsis (EONS) in newborns of serum C-reactive protein positive mothers. Study Design: Cross sectional study Place and duration of study: Department of Pediatrics, Combined Military Hospital Quetta, Pakistan from September 2016 to March 2018. Material and Methods: 105 neonates (both gender) of all mothers who were C - reactive protein positive were enrolled in this study using inclusion and exclusion criteria.Neonatal sepsis was diagnosed by clinical examination, which was carried out by senior pediatrician, blood complete picture and c reactive protein. Neonates were treated as per departmental protocol. Stratification was done in regard to gestational age, birth weight and age of neonates in hours. Post stratification chi square test was applied and p-value less than 0.05 was considered significant. Results:Out of total 105 patients, 46 (44%) were male and 59 (56%)were female neonates. Amongst the babies of 105 CRP positive mothers, 79 babies were having signs and symptoms of neonatal sepsis and 26 babies were having no signs and symptoms of neonatal sepsis with statistically significant relation between maternal CRP and neonatal sepsis of p value <0.05. Conclusion:Increased maternal CRP level is associated with increased risk of neonatal sepsis. Maternal CRP may be used as a good screening tool for early detection of neonatal sepsis.

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Evaluation of serum and salivary C-reactive protein for diagnosis of late-onset neonatal sepsis: A single center cross-sectional study

Jornal de Pediatria ◽

10.1016/j.jped.2021.01.004 ◽

2021 ◽

Author(s):

Angie M.S. Tosson ◽

Dina Koptan ◽

Rabab Abdel Aal ◽

Marwa Abd Elhady

Keyword(s):

Neonatal Sepsis ◽

Late Onset ◽

Cross Sectional Study ◽

Sectional Study ◽

Single Center ◽

C Reactive Protein ◽

Cross Sectional ◽

Reactive Protein

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Association between C - reactive protein and Premature Rupture of Membrane

Ibrahim Cardiac Medical Journal ◽

10.3329/icmj.v4i2.52988 ◽

2016 ◽

Vol 4 (2) ◽

pp. 26-31

Author(s):

Mitheel Ibna Islam ◽

Monowara Khatun ◽

Md Shahidul Islam ◽

Md Nurul Amin ◽

Shitil Ibna Islam

Keyword(s):

Amniotic Fluid ◽

Cross Sectional Study ◽

C Reactive Protein ◽

Premature Rupture ◽

College Hospital ◽

Cross Sectional ◽

Predictive Values ◽

Medical College ◽

Reactive Protein ◽

Wbc Count

Background and Objectives: Early diagnosis of clinical chorioamnionitis (ChAm) in patients with premature rupture of membrane (PROM) is essential for its prompt treatment with antibiotics. Amniocentesis may be used to detect subclinical infections in cases of PROM. But the procedure is an invasive one. The present study was undertaken to study the role of plasma C-reactive protein (CRP) in the prediction of clinical chorioamnionitis in case of PROM. Materials & Methods The cross-sectional study was carried out in the of Departments of Obstetrics & Gynaecology, Khulna Medical College Hospital, Khulna over a period 1 year from July 2014 to June 2015. A total of 90 clinically diagnosed cases of PROM (rupture of the membrane with release of the amniotic fluid more than 1 hour prior to the onset of labor) were consecutively included in the study based on predefined enrolment criteria. Clinical ChAm is defined by findings such as leukocytosis [WBC count, >15,000/µL, fetal tachycardia, maternal fever (temperature, >100.4°F), fundal or uterine tenderness, or foul-smelling amniotic fluid]. A CRP value of > 10 mg/L was considered as raised or positive CRP. The risk of developing clinical ChAm in patients with raised CRP was then estimated by computing the Odds ratio. Result: The mean age of the women with PROM was 23.9 years. The patients presented with fundal or uterine tenderness (10%), raised maternal temperature (8.9%), foetal tachycardia (10%), maternal tachycardia (13.3%), foul smelling amniotic fluid (6.7%). Over half (52.2%) of the patients were preterm PROM and the rest were term PROM. Positive CRP was found in 16.7% cases. Raised WBC count and raised ESR were found in 11.1 and 33.3% cases respectively. Over two-thirds (70%) who developed clinical chorioamnionitis had raised CRP as opposed to 10% of those who did not develop the condition. The risk of having raised CRP in patients who developed clinical chorioamnionitis was > 20-fold (4.5 - 97.7) higher than those who did not develop the condition (p < 0.001). The sensitivity and specificity of CRP in diagnosing and ruling out chorioamnionitis respectively in cases of PROM were 70% and 90% respectively. The positive and negative predictive values of the test are 46.7% and 96% respectively. Conclusion: The study concluded that a substantial proportion of the PROM cases with clinical chorioamnionitis is manifested with raised CRP compared to PROM cases without chorioamnionitis. However, CRP is moderately sensitive to diagnose chorioamnionitis and highly specific to rule out the condition in cases with PROM. Ibrahim Cardiac Med J 2014; 4(2): 26-31

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Evaluation of Procalcitonin, C-Reactive Protein, and Interleukin-6 as Early Markers for Diagnosis of Neonatal Sepsis

International Journal of Microbiology ◽

10.1155/2020/8889086 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Emad A. Morad ◽

Rehab A. Rabie ◽

Mohamed A. Almalky ◽

Manar G. Gebriel

Keyword(s):

16S Rdna ◽

Interleukin 6 ◽

Neonatal Sepsis ◽

Neonatal Intensive Care ◽

Cross Sectional Study ◽

Sepsis Group ◽

C Reactive Protein ◽

University Hospitals ◽

Cross Sectional ◽

Reactive Protein

Background. Neonatal sepsis diagnosis is a challenge because of its nonspecific presentation together with low sensitivity of the time-consuming bacterial cultures. So, many sepsis markers, like C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6), are emerging to improve its diagnosis. Aim. This study was done to investigate the role of CRP, PCT, and IL-6 in promoting the early diagnosis of neonatal sepsis in an attempt to decrease morbidity and mortality. Methods. This cross-sectional study was conducted on 50 neonates suspected with sepsis enrolled from the neonatal intensive care unit (NICU) of Zagazig University Hospitals, Egypt. Blood cultures for these neonates were done before starting antibiotics. Also, bacterial DNA was revealed from the blood by broad-range 16S rDNA polymerase chain reaction (PCR). Measurements of CRP using the immunoturbidimetry method, PCT using fluorescence immunoassay quantitative method, and IL-6 using commercially available ELISA kit were done to all enrolled neonates. Results. Forty-one neonates with proved sepsis were found to be positive in blood culture and/or PCR for bacterial 16S rDNA. The most common isolated organisms were Klebsiella (61.3%), followed by E. coli (9.7%) and CONS (9.7%). We detected much significant higher levels of PCT, CRP, and IL-6 in the proved sepsis group than the suspected neonatal sepsis cases (p≤0.001, 0.001, and 0.004, respectively). Serum PCT levels showed the highest sensitivity, specificity, PPV, NPV, and accuracy of 97.6%, 89%, 97%, 88.9%, and 96% than other studied sepsis markers. Conclusion. PCT has satisfactory characteristics as a good marker than IL-6 and CRP for the diagnosis of neonatal sepsis.

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C-Reactive Protein Testing for Active Tuberculosis among Inpatients without HIV in Uganda: a Diagnostic Accuracy Study

Journal of Clinical Microbiology ◽

10.1128/jcm.02162-20 ◽

2020 ◽

Vol 59 (1) ◽

pp. e02162-20 ◽

Cited By ~ 1

Author(s):

Amanda J. Meyer ◽

Emmanuel Ochom ◽

Patricia Turimumahoro ◽

Patrick Byanyima ◽

Ingvar Sanyu ◽

...

Keyword(s):

Diagnostic Performance ◽

Cross Sectional Study ◽

Enzyme Linked Immunosorbent Assay ◽

C Reactive Protein ◽

Likelihood Ratios ◽

Cross Sectional ◽

Predictive Values ◽

Content Type ◽

Reactive Protein ◽

Triage Test

ABSTRACTThe objective of this prospective cross-sectional study, conducted at a national referral hospital in Kampala, Uganda, was to determine diagnostic performance of serum C-reactive protein (CRP) as a triage test for tuberculosis (TB) among HIV-seronegative inpatients. We calculated the sensitivity, specificity, positive and negative likelihood ratios, and positive and negative predictive values to determine the diagnostic performance of a CRP enzyme-linked immunosorbent assay (ELISA) (Eurolyser) in comparison to that of a reference standard of Mycobacterium tuberculosis culture on two sputum samples. We constructed receiver operating curves and reported performance in reference to the manufacturer’s cutoff and also to a threshold chosen to achieve sensitivity of >90%, in accordance with the WHO’s target-product profile for a triage test. Among 119 HIV-seronegative inpatients, 46 (39%) had culture-positive pulmonary TB. In reference to M. tuberculosis culture, CRP had a sensitivity of 78% (95% confidence interval [CI], 64 to 89%) and a specificity of 52% (95% CI, 40 to 64%) at the manufacturer’s threshold of 10 mg/liter. At a threshold of 1.5 mg/liter, the sensitivity was 91% (95% CI, 79 to 98%) but the specificity was only 21% (95% CI, 12 to 32%). Performance did not differ when stratified by illness severity at either threshold. In conclusion, among HIV-seronegative inpatients, CRP testing performed substantially below targets for a TB triage test. Additional studies among HIV-seronegative individuals in clinics and community settings are needed to assess the utility of CRP for TB screening.

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Serum C - Reactive Protein Level and Peripheral Blood Picture in Children with Pneumonia

Paediatrica Indonesiana ◽

10.14238/pi38.3-4.1998.62-7 ◽

2017 ◽

Vol 38 (3-4) ◽

pp. 62

Author(s):

IGL Sidiartha ◽

BNP Arhana ◽

P Suwendra ◽

Sudaryat S

Keyword(s):

Body Temperature ◽

Peripheral Blood ◽

Cross Sectional Study ◽

C Reactive Protein ◽

Blood Picture ◽

Cross Sectional ◽

Female Ratio ◽

Reactive Protein ◽

Significant Difference ◽

Serum Crp

This cross sectional study aimed to determine the relation between serum C-reactive protein (CRP) levels and peripheral blood picture in children with bronchopneumonia, conducted at the Division of Pulmonology, Sanglah Hospital, Denpasar during 3 months (December 1996 to February 1997). Serum CRP levels, hemoglobin, leukocyte count, neutrophil count and ESR values in 30 cases between 1 month and 4.5 years (20 male and 10 female) were measured. Bronchopneumonia was mostly found in < 1 year old (67%) and with male to female ratio of 2:1. High CRP serum level (>12 microgram/ml) was found in 33% of 30 cases. Anemia was found in 37%, leukocytosis in 50%, increase segment neutrophil ratio in 30%, increase of ESR in 40% and increase of body temperature more than 38°C in 4 7% of the cases. Neutrophilia, increase of ESR and increase of body temperature were found statistically significant difference between the positive CRP and negative CRP group. Most bronchopneumonia patients in this study were probably caused by non bacteria. Neutrophilia, higher ESR and higher body temperature could be used as an indicator of bacterial infection besides the increase of serum CRP levels.

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