Immunohistochemical expression of ki-67 in odontogenic keratocyst: Evidence of aggressive behavior.

The odontogenic keratocyst (OKC) well-known for its aggressiveness and high recurrence rate, comprises approximately 11% of all jaw cysts. Due to its aggressive behavior it was placed into category of tumour in 2005 by the World Health Organization (WHO). Objectives: The purpose of this study was to determine the Ki-67 expression in Odontogenic Keratocysts to predict its proliferative potential. Study Design: Descriptive study. Setting: Department of Morbid Anatomy and Histopathology, UHS. Periods: June 2014- June 2018. Material & Methods: This is a descriptive study comprising of 39 cases of odontogenic cysts. These surgically removed samples were processed at University of Health Sciences (UHS) laboratory. Routine staining with Hematoxylin & Eosin stain along with immunohistochemistry (IHC) with Ki-67 antibody was performed. Immunohisto chemical scoring was done on the basis of percentage of the nuclear staining of Ki-67. Data was entered into SPSS 22 and descriptive statistics were measured in the form of percentage and frequency. Quantitative variables such as age of patient, size of the cyst, and Ki-67 score were also measured. P value <0.05 was taken as significant. Results: The mean age of the patients was 25.08 ±14.5 years. Significant association was observed between histological variables with odontogenic keratocyst such as parakeratinized epithelial lining (p = 0.00), epithelial hyperplasia both typical and atypical (p = 0.02) and focal spongiosis (p = 0.04). Foci having epithelial atypia demonstrated stronger staining intensity compared to adjacent normal epithelium. However, no significant association was observed between the histological variables and Ki-67 expression. Conclusion: OKC expressed low Ki-67 expression in most of the cases, however, foci of strong expression were also observed in few cases.

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Association of Iron Deficiency Anemia with Stroke Severity in patients with Acute Ischemic Stroke

10.53350/pjmhs2115113004 ◽

2021 ◽

Vol 15 (11) ◽

pp. 3004-3006

Author(s):

Rabia Rathore ◽

Nasir Farooq Butt ◽

Adil Iqbal ◽

Hina Latif ◽

Mariam Azeem ◽

...

Keyword(s):

Ischemic Stroke ◽

Iron Deficiency ◽

Acute Ischemic Stroke ◽

Iron Deficiency Anemia ◽

Descriptive Study ◽

World Health ◽

Deficiency Anemia ◽

P Value ◽

Stroke Severity ◽

Cross Sectional

Aim: To study the relationship of Iron Deficiency anemia (IDA) with severity of acute ischemic stroke. Study Design: A cross-sectional descriptive study. Place & Duration of Study: Department of Medicine, Mayo Hospital, Lahore from March 2020 to February 2021 Methods: A descriptive study of cross-sectional type was done on 200 individuals who had acute ischemic stroke (AIS) and were hospitalized at Mayo Hospital Lahore. Consecutive non-probability convenience sampling method was used to gather the data. Severity of stroke was assessed at the time of admission using the National Institute of Health Stroke Scale, (NIHSS) at the same time blood complete examination along with peripheral blood film was done to diagnose anemia in these patients. Iron studies were done to diagnose iron deficiency anemia (IDA). P-value less than 0.05 was taken as significant. Results: About 200individuals presenting with AIS were enrolled in the research work. Anemia according to World Health Organization was seen in 80(40%) and was not present in 120(60%) patients. Among the subjects who had anemia, 16(20%) had a minor AIS, 23(28.75%) had a moderately severe AIS, and 41(51.25%) reported with a severe AIS, according to NIHSS criteria. A notable relationship was found to exist between anemia and stroke severity, (P-value 0.000). Conclusion: Anemia was a commonly found in individuals with acute stroke due to ischemia and had direct relation with severity of stroke. Keywords: Iron deficiency Anemia, severity, ischemic stroke.

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Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years

South Asian Journal of Cancer ◽

10.1055/s-0040-1721212 ◽

2020 ◽

Vol 9 (02) ◽

pp. 099-104

Author(s):

Krishnendu Mondal ◽

Rupali Mandal ◽

Badal Chandra Sarkar

Keyword(s):

Verrucous Carcinoma ◽

Statistical Significance ◽

Normal Mucosa ◽

Oral Leukoplakia ◽

World Health ◽

P Value ◽

Severe Dysplasia ◽

Ki 67 ◽

Mild Dysplasia ◽

Tissue Sections

Abstract Background Early detection of dysplastic changes within oral potentially malignant disorders is the mainstay to prevent oral cancer. Ki-67 is one of the most useful antigens in this purpose. Aims The study aims were to recognize and mutually compare the proliferative status of idiopathic oral leukoplakia (OL) patches, which presented through different forms of dysplasia and carcinoma. Settings and Design In 4 years of observation, cumulatively 140 OL lesions were included for examination. The wholesome Ki-67 labeling scores in each of the subgroups were calculated. Subjects and Methods The World Health Organization recommended histopathological classification was used to categorize the dysplastic and malignant lesions. Paraffin-embedded tissue sections were processed for Ki-67 immunostaining. The labeling indices (LIs) were quantified semiquantitatively at the site of maximal reactive cells on tissue sections. Statistical Analysis The statistical comparison was performed by means of the SPSS software (Version 16.0 SPSS Inc.). A p-value < 0.05 was considered as the benchmark for statistical significance. Results A steady and significant increment in Ki-67 expression was discovered from dysplastic to malignant OL patches compared with normal mucosa. The labeling differences were significant between normal mucosa and mild dysplasia, as well as between mild, moderate, and severe dysplasia. However, the expression did not differ significantly with the severity of oral cancers. Conclusions Ki-67 is a useful molecular marker of carcinogenesis in OL. It also serves worthwhile in separating marginally dysplastic lesions, such as mild dysplasia or verrucous carcinoma from their benign epigones.

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STUDI PENGARUH STATUS PERKAWINAN DAN PEKERJAAN PADA PASIEN TUBERKULOSIS DI RUMAH SAKIT DKT PURWOKERTO

Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan ◽

10.35960/vm.v10i2.438 ◽

2019 ◽

Vol 10 (2) ◽

pp. 46-51

Author(s):

Peppy Octaviani

Keyword(s):

Infectious Disease ◽

Developing Countries ◽

Pulmonary Tuberculosis ◽

Descriptive Study ◽

World Health ◽

P Value ◽

Cross Sectional ◽

Pulmonary Tb ◽

The World ◽

Health Organization

ABSTRACT Pulmonary tuberculosis (TB) is a contagious pulmonary infectious disease that is still a health problem in the world, especially developing countries. Tuberculosis has been proclaimed by WHO (World Health Organization) as Global Emergency since 1992. The purpose of this study is to find out what physical characteristics are at risk of tuberculosis in DKT Hospital Purwokerto. The research design used in this study was a descriptive study with a cross-sectional approach to determine the characteristics of pulmonary TB patients who were adherent to treatment and those who did not comply with treatment at the DKT Hospital in Purwokerto. This research was conducted at the DKT Purwokerto Hospital in May 2018. The samples studied in this study were pulmonary TB patients who were obedient to treatment and non-compliance with treatment at the DKT Purwokerto Hospital for the period of 1 January - 30 December 2017 that met the sample criteria. The results of the study have no relationship between age and the results of sputum examination at the Purwokerto DKT Hospital (p value = 0.286), there is no relationship between sex with the results of sputum examination at DKT Purwokerto Hospital (p value = 0.261). Keywords: Pulmonary TBC, Characteristics, Phlegm Examination

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Combined conservative treatment of a recurrent mandibular keratocyst by enucleation, diode laser, 5-Fluorouracil and dental implants: a case report with 18 years-follow up

Medicine and Pharmacy Reports ◽

10.15386/mpr-2067 ◽

2021 ◽

Author(s):

Antoine Berberi ◽

Georges Aoun ◽

Bouchra Hjeij ◽

Maissa AboulHosn

Keyword(s):

Diode Laser ◽

World Health ◽

Odontogenic Keratocyst ◽

High Recurrence Rate ◽

Complete Excision ◽

Carnoy’S Solution ◽

The World ◽

Chemical Solutions ◽

Health Organization

In 2017, the World Health Organization classified the odontogenic keratocyst as a developmental odontogenic cyst. The posterior parts of the mandible, especially the angle and the ramus, are the most involved sites. Due to their high recurrence rate, keratocysts are managed surgically with careful complete excision. Additionally, chemical solutions such as Carnoy's solution, modified Carnoy's solution, and 5-Fluorouracil have been associated with surgical treatment. Diode lasers generate diverse wavelengths that have photothermal and photochemical special properties and could have some effects on the lining epithelium remnants of the cyst. In this paper, we discuss a case of mandibular recurrent odontogenic keratocyst treated with 5-Fluorouracil topical application after enucleation and diode laser application with 18 years follow-up and teeth replacement with dental implant. 5-Fluorouracil and diode laser have been found to be very effective in the treatment of keratocysts.

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Immediate Implants After Enucleation of an Odontogenic Keratocyst: An Early Return to Function

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-10-00027 ◽

2012 ◽

Vol 38 (S1) ◽

pp. 485-488 ◽

Cited By ~ 1

Author(s):

Sabri C. Isler ◽

Sabit Demircan ◽

Taylan Can ◽

Zerrin Cebi ◽

Emrah Baca

Keyword(s):

Aggressive Behavior ◽

Dental Implants ◽

Recurrence Rate ◽

Male Patient ◽

Odontogenic Keratocyst ◽

High Recurrence Rate ◽

Histologic Appearance ◽

Early Return ◽

Immediate Implants

An odontogenic keratocyst is a unique cyst because of its locally aggressive behavior, high recurrence rate, and characteristic histologic appearance. In this article we present the case of a 22-year-old male patient with a large odontogenic keratocyst and describe his treatment with immediate dental implants.

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Immunohistochemical expression of Cyclooxygenase 2 reflects the proliferative activity in the epithelium of odontogenic lesions

Dentistry 3000 ◽

10.5195/d3000.2022.205 ◽

2022 ◽

Vol 10 (1) ◽

Author(s):

Vani Verma ◽

Chetana Chandrashekar ◽

Raghu Radhakrishnan ◽

Monica Charlotte Solomon

Keyword(s):

Epithelial Cells ◽

Dentigerous Cyst ◽

Cyclooxygenase 2 ◽

Odontogenic Keratocyst ◽

Odontogenic Cysts ◽

Radicular Cyst ◽

Chi Square ◽

Ki 67 ◽

Odontogenic Epithelium ◽

Cox 2

Purpose: Odontogenic cysts and tumors comprise a major component of lesions of the oral and maxillofacial region. The pathogenesis of these lesions involves the interaction between the odontogenic epithelium and the ectomesenchyme. However, the clinical behavior of these biological entities is unpredictable. The aim of this study was to evaluate the role of Cyclooxygenase 2 (COX-2) in the pathogenesis and prognostication of odontogenic lesions.Material and method: : In this study formalin-fixed paraffin-embedded tissue section of Odontogenic Keratocyst (n=10) Dentigerous cyst (n=10), Radicular cyst (n=10) and unicystic ameloblastoma (n=10) were immunohistochemically stained with COX-2 (NCL2-COX-2- 4H12) and with Ki 67 (Ki-67 GM001) using standard staining protocols. The cytoplasmic expression of COX-2 in all the lesions was semi-quantitatively assessed. The pattern of expression of COX-2 among the different odontogenic lesions was statistical analyzed using the ANOVA test and the chi-square test.Results: All the 40 odontogenic lesions that were evaluated expressed COX-2 immunohistochemically. A high number of odontogenic epithelial cells expressed COX-2 in most of the odontogenic keratocyst, radicular cyst and unicystic ameloblastomas. The expression of COX-2 was significantly (p=0.036) higher in Unicystic Ameloblastomas and Radicular cyst compared to that of Odontogenic Keratocyst and the dentigerous cyst.Conclusion: The recognition that expression of COX-2 by odontogenic epithelial cells may indeed shed a new light on the biological mechanisms involved in the development of these benign yet aggressive lesions of the jaws. An insight into the molecular interactions occurring in the odontogenic epithelium will aid in better management of these lesions.

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Assessment of Proliferative Potential of Odontogenic Keratocyst and Dentigerous Cyst using Podoplanin: An Immunohistochemical Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-2194 ◽

2017 ◽

Vol 18 (12) ◽

pp. 1173-1176 ◽

Cited By ~ 1

Author(s):

Prashant Rao ◽

Aparna Paliwal ◽

Shekhar Grover ◽

Sandeep Gupta ◽

Nidhi Choudaha

Keyword(s):

Immunohistochemical Study ◽

Dentigerous Cyst ◽

Odontogenic Keratocyst ◽

Odontogenic Cysts ◽

Proliferative Potential ◽

Positive Staining ◽

The Social ◽

Suprabasal Cell ◽

Cell Layers ◽

Podoplanin Expression

ABSTRACT Introduction Odontogenic cysts are commonly encountered lesions among head and neck pathologies. Odontogenic keratocyst (OKC) has unique features of recurrence and local aggressiveness. Podoplanin (PDP) is a lymphatic endothelial marker and is shown to be expressed in a variety of tissues. Hence, we planned to assess the significance of PDP in OKC and dentigerous cyst (DC). Materials and methods The present study included assessment of immunoexpression of PDP in OKC and DC. Twenty specimens each of OKC and DC were included in the present study and were stained with D2-40 antibody. All the sections were analyzed and were categorized as negative staining, weakly positive staining, and strongly positive staining. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software. Results We detected PDP-positive staining in the cell membrane and cytoplasm of the cells of basal cell layer and suprabasal cell layers. In DC cases, we observed positive staining only in cases associated with inflammation. Conclusion Podoplanin does play a significant role in enhancing the local invasive and neoplastic properties of OKC. Clinical significance Podoplanin expression in OKC is potentially associated with moderate invasive nature of the neighboring structures. How to cite this article Gupta S, Paliwal A, Choudaha N, Gupta A, Rao P, Grover S. Assessment of Proliferative Potential of Odontogenic Keratocyst and Dentigerous Cyst using Podoplanin: An Immunohistochemical Study. J Contemp Dent Pract 2017;18(12):1173-1176.

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Survivin as a Useful Adjunct Marker for the Grading of Papillary Urothelial Carcinoma

Archives of Pathology & Laboratory Medicine ◽

10.5858/2008-132-224-saauam ◽

2008 ◽

Vol 132 (2) ◽

pp. 224-231

Author(s):

Ying-bei Chen ◽

Jiangling J. Tu ◽

Jean Kao ◽

Xi K. Zhou ◽

Yao-Tseng Chen

Keyword(s):

Urothelial Carcinoma ◽

Messenger Rna ◽

Interobserver Variability ◽

World Health ◽

Low Grade ◽

Nuclear Staining ◽

High Grade ◽

Grade Group ◽

Ki 67 ◽

Papillary Urothelial Carcinoma

Abstract Context.—Distinguishing low-grade and high-grade noninvasive papillary urothelial carcinoma based on morphologic criteria can be challenging and adjunct markers are highly desirable. Survivin, presumably an antiapoptotic protein, was previously proposed as a prognostic marker for urothelial carcinoma. Objective.—To assess interobserver variability by 2004 World Health Organization classification and the value of survivin and Ki-67 as potential markers for grading noninvasive papillary urothelial carcinoma. Design.—Fifty-one bladder biopsies were graded blindly by 5 experienced general surgical pathologists. The protein and messenger RNA expression of survivin and Ki-67 was evaluated by immunohistochemistry and quantitative reverse transcription–polymerase chain reaction using paraffin-embedded tissue. The immunohistochemistry result was quantitatively analyzed using a computer-based color deconvolution module. Results.—The diagnostic agreement among 5 pathologists was fair to poor, with 32% of the cases graded differently by at least 2 raters. All cases were divided into 3 groups: consensus low-grade, consensus high-grade, and indeterminate. The percentage of urothelial cells with positive survivin nuclear staining (survivin score) was significantly higher in the high-grade than in the low-grade group (P < .001). Survivin score outperformed Ki-67 in separating the high-grade group from the low-grade group and showed a significantly higher predictive accuracy for high-grade recurrence than the histologic grade. The disagreement of grading for the indeterminate group could be resolved by their survivin scores in most cases. Survivin messenger RNA level correlated well with survivin score by immunohistochemistry but was not a more discriminating marker. Conclusions—Significant interobserver variability exists in grading low-grade versus high-grade papillary urothelial carcinoma. Survivin immunohistochemical staining can be a useful adjunct tool for the grading of challenging cases.

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STUDI PENGARUH UMUR DAN JENIS KELAMIN PADA PASIEN TUBERKULOSIS DI RUMAH SAKIT DKT PURWOKERTO

Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan ◽

10.35960/vm.v10i2.437 ◽

2019 ◽

Vol 10 (2) ◽

pp. 40-45

Author(s):

Peppy Octaviani

Keyword(s):

Infectious Disease ◽

Developing Countries ◽

Pulmonary Tuberculosis ◽

Descriptive Study ◽

World Health ◽

P Value ◽

Cross Sectional ◽

Pulmonary Tb ◽

The World ◽

Health Organization

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A clinicopathological study and management of odontogenic keratocyst

Update Dental College Journal ◽

10.3329/updcj.v9i1.41200 ◽

2019 ◽

Vol 9 (1) ◽

pp. 8-15

Author(s):

Mohammad Asifur Rahman ◽

Tarin Rahman ◽

Ismat Ara Haider

Keyword(s):

Conservative Treatment ◽

Recurrence Rate ◽

Surgical Resection ◽

Treatment Options ◽

Anatomical Structure ◽

Odontogenic Keratocyst ◽

High Recurrence Rate ◽

Odontogenic Cysts ◽

Odontogenic Keratocysts ◽

Surgical Methods

Odontogenic Keratocyst is an aggressive odontogenic cyst with a high recurrence rate. After radicular and follicular cysts, odontogenic keratocysts are the third most common cyst of the jaws and approximately 12-14% of all odontogenic cysts. It has been retermed to Keratocystic odontogenic tumour (KCOT) as it better reflects its neoplastic nature but recently it has been re classified and retermed into the cystic category. Various surgical methods have been proposed but comparatively, conservative treatment options such as Dredging methods might be the treatment of choice due to preservation of anatomical structure. Objective: The aim of this study was to analyse the clinical, radiological and histopathological characteristics of Odontogenic Keratocyst and provide a proper management system affected by this type of lesions. Materials and methods: The prospective study was performed in Dhaka Dental College and Hospital from a period of January 2014 to January 2018. A total number of 75 patients were selected for this study based on clinical, radiological and histopathological confirmation of odontogenic keratocysts. The treatment options were enucleation, enucleation with curettage, enucleation with peripheral ostectomy, Dredging method and surgical resection. After treatment patients were followed up 1months, 3 months and 6 months in every year at least for 5 years. Results: Among 75 patient of odontogenic keratocyst; the mean age was 27.69±13.35 and age range was 11 to 66 years. Male were 53(71%) and 22 (29%) were female patients. 53 (70.67%) cases were found in the mandible, 15(20%) cases in the maxilla and in 7(9.33%) cases were involved in both maxilla and mandible; mandibular posterior region was the most specific region involved 37(69.81%).The most common clinical features revealed pain and swelling. Radiologically, 70.66% unilocular, 96% well defined and 94.66% radiolucent area were prominent. Bone expansion 37.38%, root resorption 30.00% and 36% were associated with an impacted tooth. Regarding treatment options enucleation with curatage 12%, enucleation, curettage & peripheral ostectomy 29.33%, Dredging 52% and surgical resection 6.6% was done. Recurrence occurred in 18 patients with recurrence rate of 24%. Conclusion: Odontogenic keratocyst is an aggressive cyst, male predominant, posterior mandible is the commonest site and well defined unilocular radiolucency are commonest radiological feature. Radical treatment options such as resection reduced the recurrences of the tumour but higher morbidity and jaw deformity. Comparatively, conservative treatment options such as Dredging methods might be the treatment of choice due to preservation of anatomical structure. A long term follow up is paramount importance for the research and understanding the clinical pattern, behavior, treatment and recurrence of the lesion. Update Dent. Coll. j: 2019; 9 (1): 8-15

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