scholarly journals Uji Kualitatif dan Uji Aktivitas Antioksidan Ekstrak Etanolik Buah Maja (Aegle Marmelos (L.)Correa) dengan Metode DPPH

2021 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Muhammad Nur Fauzi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Aegle Marmelos ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
The Third ◽  
Dpph Method ◽  
Fruit Samples ◽  
The World

Abstract. Indonesia is a country with the third largest tropical forest in the world. The number of medicinal plants in Indonesia is estimated to be around 1,260 types of plants. Plants produce secondary metabolites that have potential as antioxidants. One of the plants that contains a lot of secondary metabolites is maja (Aegle marmelos (L.) Corr). The purpose of this study was to determine the content of secondary metabolites in maja fruit extracts and to determine the antioxidant activity contained in maja fruit extracts using the DPPH method. The research was conducted by extracting maja fruit samples using maceration method to obtain a thick extract. The extracts obtained were tested for secondary metabolites, TLC test, and antioxidant activity tests using the DPPH method using Uv-vis spectrophotometry. The results of this study indicate that maja fruit extract contains secondary metabolites of flavonoids, tannins, alkaloids, saponins, and glycosides. TLC results obtained Rf 0.512. The result of antioxidant activity of maja fruit extract obtained by IC50 was 269.153 µg / mL. and IC50 vitamin C as a comparison obtained 28,907µg / mL. This shows that the antioxidant activity of maja fruit extract is smaller than the antioxidant activity of vitamin C. Abstrak. Indonesia adalah negara dengan hutan tropis paling besar ketiga di dunia, Jumlah tumbuhan berkhasiat obat di Indonesia diperkirakan sekitar 1.260 jenis tumbuhan. Tumbuhan menghasilkan metabolit sekunder yang berpotensi sebagai antioksidan. Salah satu tanaman yang banyak mengandung metabolit sekunder adalah tanaman maja (Aegle marmelos (L.) Corr). Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder pada ekstrak buah maja dan untuk mengetahui aktivitas antioksidan yang terdapat pada ekstrak buah maja dengan metode DPPH. Penelitian dilakukan dengan mengekstraksi sampel buah maja dengan metode maserasi untuk memperoleh ekstrak kental. Ekstrak yang diperoleh dilakukan uji metabolit sekunder, uji KLT, dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri Uv-vis. Hasil penelitian ini menunjukkan bahwa ekstrak buah maja mengandung metabolit sekunder flavonoid, tanin, alkaloid, saponin, dan glikosida. Hasil KLT diperoleh Rf 0,512. Hasil aktivitas antioksidan ektrak buah maja yang diperoleh dengan IC50 adalah 269,153 µg/mL. dan IC50 vitamin c sebagai pembanding diperoleh 28,907µg/mL. hal ini menunjukan bahwa daya aktivitas antioksidan ekstrak buah maja lebih kecil dibanding dengan daya aktivitas antioksidan vitamin C. Kata Kunci: , , 

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Nery Sofiyanti ◽  
Rodesia Mustika Roza ◽  
Isnaini Isnaini ◽  
Yulisa Resti Irawan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Natural Resources ◽  
The Other ◽  
Distilled Water ◽  
Water Solvent ◽  
Activity Test ◽  
Dpph Method ◽  
Riau Archipelago

<p><em>Obat Pahit</em> is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely <em>Bauhunia semibifida, Cnestis palala</em> and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in <em>Obat Pahit</em> from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of <em>Obat Pahit</em> from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of <em>Bauhinia semibifida</em> (6.6247), Penawa Root (5.0124) and <em>Cnestis palala</em> (5.9968) were much lower than IC50 of mangosteen’s rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia’s local ethnicities.</p>

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSRTRAK LUMUT HATI (Marchantia polymorpha)

KOVALEN ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 315-321
Author(s):  
Nurhaeni Nurhaeni ◽  
Gladys ◽  
Jaya Hardi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Hexane Extract ◽  
Marchantia Polymorpha ◽  
Dpph Method ◽  
Ethanol Fraction

The research is about antioxidant activity of the liverworts (Marchatia polymorpha) extract based on the level of a polar solvent. This research was done by maceration of liverworts by using n-hexane, ethyl acetate and ethanol solvent. The n-hexane, ethyl acetate, and ethanol fraction were tested the antioxidant activity by using DPPH method. The result was obtained for antioxidant activity (IC50) on n-hexane extract, ethyl acetate, ethanol, and vitamin C is 1065.33 ppm, 1326.52 ppm, 1876.11 ppm, and 13.45 ppm, respectively. The liverworts extract was identified contains secondary metabolites compounds of flavonoid, tannins, polyphenol, alkaloid, and steroid. Keywords: Liverwort extract, antioxidant, IC50

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Kluwih (Artocarpus Communis)

2018 ◽  
Vol 7 (2) ◽  
pp. 85
Author(s):  
Mohammad Arif ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Vitamin C ◽  
Positive Control ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ic50 Value ◽  
Fruit Powder ◽  
Central Sulawesi

This study aimed to determine antioxidant activity of artocarpus camansi fruit extracts from Sigi, Central Sulawesi. This study was conducted by maceration extraction technique, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) as the source of free radicals, and vitamin C as a positive control. UV-Vis spectrophotometer was used for measuring the absorbance of artocarpus camansi fruit extracts. artocarpus camansi fruit powder was extracted by maceration using ethanol. Various concentrations of artocarpus camansi fruit extracts used were 20, 40, 60, and 80 mg/L. The results showed that the IC50 value for artocarpus camansi (artocarpus communis) fruit extract was 88.715 mg/L, whereas the IC50 value for vitamin C was 37.153 mg/L. Artocarpus camansi (artocarpus communis) fruit extracts are powerful antioxidants category based on IC50 value. The optimum percentage antioxidant activity of artocarpus camansi fruit extracts to inhibit free radical amounted to by 50.65%

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test. The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals Determination of total phenolic content and antioxidant activity of fruit mix from Malino, Gowa Regency, South Sulawesi

2021 ◽  
Vol 911 (1) ◽  
pp. 012085
Author(s):  
Risma Eva Cahyanti ◽  
Yusminah Hala ◽  
A. Mu’nisa
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Passion Fruit ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Purple Passion Fruit

Abstract This study aimed to determine the total phenolic content and antioxidant activity of single fruit extracts and mixtures of tomatoes (Solanum lycopersicum), purple passion fruit (Passiflora edulis var. Sims), and strawberries (Fragaria sp.). Tomatoes, purple passion fruit and strawberries were extracted using 96% ethanol as solvent using the maceration method. Determination of total phenolic content using the Folin-ciocalteu method, measurement of antioxidant activity using the DPPH method (1.1 diphenyl-2-picrylhydrazil) spectrophotometrically and measuring the degree of acidity (pH) using a pH meter. Data were analyzed using ANOVA with Tukey’s further test. The results showed that the total phenolic content of tomatoes, purple passion fruit and strawberries before and after mixing were tomato extract (1,731 mg GAE/g), purple passion fruit extract (1,577 mg GAE/g), strawberry extract (1,917 mg GAE)./g), tomato and purple passion fruit extract (1,758 mg GAE/g), tomato and strawberry extract (2,020 mg GAE/g), strawberry and purple passion fruit extract (1,924 mg GAE/g) and tomato, purple passion fruit extract and strawberries (2.107 mg GAE/g). The antioxidant activity showed that there was a significant difference between the purple passion fruit treatments (78.695%), tomatoes and strawberries (86.160%) and tomatoes, purple passion fruit and strawberries (88.328%), but not significantly different from the tomatoes (80.683 %), tomatoes and purple passion fruit (82,059 %) as well as the treatment of strawberries (83.690 %), strawberries and purple passion fruit (84.097 %), but significantly different from the BHA control (93.526 %). It can be concluded that the total phenolic content and antioxidant activity of the mixed extracts of the three fruits, namely tomatoes, purple passion fruit and strawberries, were higher than those of the single fruit extracts without mixing.

Development of Gel Dossage Form From Mulberry Fruit Extract as A Facial Treatment

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Dhanesh Arif ◽  
Diah Lia Aulifa ◽  
Driyanti Rahayu ◽  
Arni Praditasari
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Vitamin C ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Microdilution Method ◽  
Mulberry Fruit ◽  
Ic50 Value ◽  
Dpph Method

Skin care is very important in cosmetics, especially facial treatments. Black mulberry is rich in phenols and is therefor usable in the treatment of acne. It also contains anthocyanin, a well-known antioxidant and a potential source of sun protection. This research aimed to develop a gel dossage from black mulberries (Morus nigra) extracts that would function as an antibacterial, antioxidant and sun blocking facial treatment. This research started with black mulberry fruit extracted through the maceration method by using ethanol (96%). Then, the antibacterial activity of the extract was determined by the disc-diffusion method, while the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the microdilution method. Antioxidant activity of the extract was determined using the 1,1- diphenyl-2-picrilhidrazil (DPPH) method with vitamin C as a comparison. Furthermore, the extracts were formulated into gel formulas with variations of HPMC, Na-CMC, carbopol 934 and extract concentrations. The products were then physically evaluated for organoleptics, homogeneity, pH, viscosity, and dispersion as well as undergoing a hedonic test, an irritation test, an antioxidant activity test and determining the SPF value of the preparation. The results showed that the black mulberry fruit extract has antibacterial activity with MIC value of 0,025 g/mL against S. epidermidis and P. acnes, while MBC values were 0,025 g/mL and 0,05 g/mL, respectively. The black mulberry extract had antioxidant activity with IC50 value, i.e 146,73 μg/mL vitamin c i.e. 3,17 μg/mL. Formulation with best physical evaluation results showed ina formula containing a carbopol gel base of 934 0,015 g/mL with an extract cconcentration of 0,075 g/mL. This formula resulted in antioxidant activity with an IC50 value, i.e 1004,6 μg/mL, antibacterial activity with inhibition zone 6.83 ± 1.4 mm against and 6.76 ± 0.9 mm against S. epidermidis and P. acnes respectively and an SPF value of 1.9.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BUAH PURNAJIWA (Kopsia arborea Blume.) DENGAN BERBAGAI PELARUT

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 24 ◽  
Author(s):  
Didit Purwanto ◽  
Syaiful Bahri ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Fruit Extract ◽  
Dpph Method ◽  
Ic50 Values

Antioxidant activity of Purnajiwa (Kopsia arborea Blume.) fruit has been investigated.The fruit was extracted by various solvents such as n-hexane, ethyl acetate, and ethanol. The aim of the research is to define a class of compounds and antioxidant activity. The compounds test was conducted by the phytochemical method. The results showed that the purnajiwa fruit extract is classified as secondary metabolites of flavonoids, saponins, tannins, alkaloids, and steroids. The antioxidant activity was tested by DPPH method and the amount of antioxidant activity was characterized by IC50 values. The result indicated that the antioxidant activity (IC50) for n-hexane, ethyl acetate, and ethanol solvents is 3524.05 ppm, 316.09 ppm, and 154.89 ppm respectively.Keywords: Purnajiwa Fruit, Antioxidant, DPPH, Phytochemicals

scholarly journals Antioxidant Activity Saripati Beruwas Laut Fruit (Scaevola taccada)

2021 ◽  
Vol 324 ◽  
pp. 01012
Author(s):  
Kiki Fatmawati ◽  
Azwin Apriandi ◽  
Sri Novalina Amrizal ◽  
Zubaidah Anjar Rezeki
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Sandy Soil ◽  
Water Extract ◽  
Fruit Extract ◽  
Stable Foam ◽  
Young Fruit ◽  
Dpph Method ◽  
Coastal Plant ◽  
Young Old

Scaevola taccada is a coastal plant. This plant lives in gravel sandy soil and can prevent erosion that occurs on the beach. In the Riau Islands, it is still very rare, not even a few do not know the benefits of this plant, especially the fruit. The study aimed to determine the antioxidant activity of young, old, and mixed fruit extracts of S. taccada using the DPPH method which was stated to be IC50. The research methods include extraction, phytochemical test, antioxidant test and vitamin C test. The yield of pulp and water extract of young fruit was 55% and 41.4%, pulp and water extract of old fruit was 33.33% and 42.9%, respectively. Phytochemical bioactive analysis was found to be positive for saponin parameters, indicated by the presence of stable foam for 30 minutes. Antioxidant activity using DPPH method obtained IC50 19,524 young fruit extract, IC50 50,664 old fruit extract, and IC50 35.518 mixed fruit. Analysis of Vitamin C in old S. taccada fruit yielded 5.28 mg, young fruit yielded 20.24 mg, and mixed fruit yielded 35.20 mg. The conclusion of this study is the presence of antioxidants and vitamin C in Scaevola taccada fruit.

scholarly journals Antioxidant Activity of Fruit Extract Powder Beans (Phaseolus vulgaris L.) Using DPPH Method

2020 ◽  
Vol 6 (2) ◽  
pp. 194-198
Author(s):  
Rizki Nugrahani ◽  
Yayuk Andayani ◽  
Aliefman Hakim
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phaseolus Vulgaris ◽  
Secondary Metabolites ◽  
Oxidation Reaction ◽  
Phytochemical Screening ◽  
Phaseolus Vulgaris L ◽  
Fruit Extract ◽  
Dpph Method ◽  
Vis Spectroscopy

Free radical effect is one of many factors that can cause coronic desease, heart coronary desease, diabetes militus.  Beans plant (Phaseolus vulgaris L.) countant antioxidant compound. Antioxidants could inhibit oxidation reaction, that is cousing free radical compounds. The objective of this research was to determine the potential of bean extracts as an antioxidant. DPPH method was used to measure antioxidant activity, absorbances was measured using UV-Vis spectroscopy. Scrining phytochemical used to identified secondary metabolites in samples. The result showed IC50 1268.18; 2512; 1698.18 and 4442.75 μg/mL for all variety of stroge (less 1, 1, 2, 3 months) and IC50 control BHT was 1.744 μg/mL. Extract of bean powder sample classified as very weak antioxidant activity, variety of storage could effect to antioxidant activity. Phytochemical screening results obtained flavonoids, phenols, alkaloids, saponins, steroids and terpenoids present in the sample, while tannin are not included in the sample

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