scholarly journals Antioxidant Activity of Fruit Extract Powder Beans (Phaseolus vulgaris L.) Using DPPH Method

2020 ◽  
Vol 6 (2) ◽  
pp. 194-198
Author(s):  
Rizki Nugrahani ◽  
Yayuk Andayani ◽  
Aliefman Hakim
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phaseolus Vulgaris ◽  
Secondary Metabolites ◽  
Oxidation Reaction ◽  
Phytochemical Screening ◽  
Phaseolus Vulgaris L ◽  
Fruit Extract ◽  
Dpph Method ◽  
Vis Spectroscopy

Free radical effect is one of many factors that can cause coronic desease, heart coronary desease, diabetes militus.  Beans plant (Phaseolus vulgaris L.) countant antioxidant compound. Antioxidants could inhibit oxidation reaction, that is cousing free radical compounds. The objective of this research was to determine the potential of bean extracts as an antioxidant. DPPH method was used to measure antioxidant activity, absorbances was measured using UV-Vis spectroscopy. Scrining phytochemical used to identified secondary metabolites in samples. The result showed IC50 1268.18; 2512; 1698.18 and 4442.75 μg/mL for all variety of stroge (less 1, 1, 2, 3 months) and IC50 control BHT was 1.744 μg/mL. Extract of bean powder sample classified as very weak antioxidant activity, variety of storage could effect to antioxidant activity. Phytochemical screening results obtained flavonoids, phenols, alkaloids, saponins, steroids and terpenoids present in the sample, while tannin are not included in the sample

scholarly journals Uji Kualitatif dan Uji Aktivitas Antioksidan Ekstrak Etanolik Buah Maja (Aegle Marmelos (L.)Correa) dengan Metode DPPH

2021 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Muhammad Nur Fauzi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Aegle Marmelos ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
The Third ◽  
Dpph Method ◽  
Fruit Samples ◽  
The World

Abstract. Indonesia is a country with the third largest tropical forest in the world. The number of medicinal plants in Indonesia is estimated to be around 1,260 types of plants. Plants produce secondary metabolites that have potential as antioxidants. One of the plants that contains a lot of secondary metabolites is maja (Aegle marmelos (L.) Corr). The purpose of this study was to determine the content of secondary metabolites in maja fruit extracts and to determine the antioxidant activity contained in maja fruit extracts using the DPPH method. The research was conducted by extracting maja fruit samples using maceration method to obtain a thick extract. The extracts obtained were tested for secondary metabolites, TLC test, and antioxidant activity tests using the DPPH method using Uv-vis spectrophotometry. The results of this study indicate that maja fruit extract contains secondary metabolites of flavonoids, tannins, alkaloids, saponins, and glycosides. TLC results obtained Rf 0.512. The result of antioxidant activity of maja fruit extract obtained by IC50 was 269.153 µg / mL. and IC50 vitamin C as a comparison obtained 28,907µg / mL. This shows that the antioxidant activity of maja fruit extract is smaller than the antioxidant activity of vitamin C. Abstrak. Indonesia adalah negara dengan hutan tropis paling besar ketiga di dunia, Jumlah tumbuhan berkhasiat obat di Indonesia diperkirakan sekitar 1.260 jenis tumbuhan. Tumbuhan menghasilkan metabolit sekunder yang berpotensi sebagai antioksidan. Salah satu tanaman yang banyak mengandung metabolit sekunder adalah tanaman maja (Aegle marmelos (L.) Corr). Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder pada ekstrak buah maja dan untuk mengetahui aktivitas antioksidan yang terdapat pada ekstrak buah maja dengan metode DPPH. Penelitian dilakukan dengan mengekstraksi sampel buah maja dengan metode maserasi untuk memperoleh ekstrak kental. Ekstrak yang diperoleh dilakukan uji metabolit sekunder, uji KLT, dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri Uv-vis. Hasil penelitian ini menunjukkan bahwa ekstrak buah maja mengandung metabolit sekunder flavonoid, tanin, alkaloid, saponin, dan glikosida. Hasil KLT diperoleh Rf 0,512. Hasil aktivitas antioksidan ektrak buah maja yang diperoleh dengan IC50 adalah 269,153 µg/mL. dan IC50 vitamin c sebagai pembanding diperoleh 28,907µg/mL. hal ini menunjukan bahwa daya aktivitas antioksidan ekstrak buah maja lebih kecil dibanding dengan daya aktivitas antioksidan vitamin C. Kata Kunci: , , 

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

scholarly journals Phenolic compounds and antioxidant activity of two bean cultivars (Phaseolus vulgaris L.) submitted to cooking

2017 ◽  
Vol 21 (0) ◽  
Author(s):  
Maira Oliveira Silva ◽  
Priscila Brigide ◽  
Nataly Maria Viva de Toledo ◽  
Solange Guidolin Canniatti-Brazaca
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Thermal Processing ◽  
Sulphonic Acid ◽  
Phaseolus Vulgaris L ◽  
Dpph Method ◽  
The Common

Abstract The common bean (Phaseolus vulgaris L.) is a source of nutrients and contains phenolic compounds that act as antioxidants. The aim of the present study was to determine the phenolic compounds and tannins in two bean cultivars (Phaseolus vulgaris L.): the biofortified carioca bean (Pontal) and the common bean (commercial). The antioxidant activity of the phenolic compounds and their fractions was also measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. The thermal processing decreased the phenolic compounds, tannins and the antioxidant activity of beans. The Pontal cultivar exhibited higher levels of phenolic compounds even after cooking. For cooked beans, higher antioxidant activity was observed in the commercial beans by the DPPH method. Regarding to the fractions, in general, lower values of antioxidant activity by DPPH were observed for beans after cooking, except for fraction 6 of the Pontal bean and fraction 3 of the commercial bean. For fraction 4 no significant differences were observed by the ABTS method for both cultivars after thermal processing.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BUAH PURNAJIWA (Kopsia arborea Blume.) DENGAN BERBAGAI PELARUT

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 24 ◽  
Author(s):  
Didit Purwanto ◽  
Syaiful Bahri ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Fruit Extract ◽  
Dpph Method ◽  
Ic50 Values

Antioxidant activity of Purnajiwa (Kopsia arborea Blume.) fruit has been investigated.The fruit was extracted by various solvents such as n-hexane, ethyl acetate, and ethanol. The aim of the research is to define a class of compounds and antioxidant activity. The compounds test was conducted by the phytochemical method. The results showed that the purnajiwa fruit extract is classified as secondary metabolites of flavonoids, saponins, tannins, alkaloids, and steroids. The antioxidant activity was tested by DPPH method and the amount of antioxidant activity was characterized by IC50 values. The result indicated that the antioxidant activity (IC50) for n-hexane, ethyl acetate, and ethanol solvents is 3524.05 ppm, 316.09 ppm, and 154.89 ppm respectively.Keywords: Purnajiwa Fruit, Antioxidant, DPPH, Phytochemicals

scholarly journals Phytochemicals and GC-MS of Aquilaria beccariana van Tiegh leaves growing naturally and cultivated in North Sumatera

2021 ◽  
Vol 912 (1) ◽  
pp. 012079
Author(s):  
R Batubara ◽  
W Pulungan ◽  
T I Hanum ◽  
O Affandi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method

Abstract Aquilaria beccariana van Tiegh is one of the agarwood (gaharu) species that grows naturally and cultivatived. However, the chemical and antioxidant potential of its leaves have not been studied much. This research aimed to examine the chemical compounds and the antioxidant activity of the ethanol extract of the agarwood leaves A. beccariana van Tiegh which grows naturally and cultivatived. The leaves were processed into simplicia and then extracted using 96% ethanol as solvent. Phytochemical screening agarwood leaves to determine the chemical compounds such as of alkaloids, flavonoids, glycosides, steroids/triterpenoids, saponins and tannis. The antioxidant activity test was carried out using the DPPH method to obtain the IC50 (Inhibitory Concentration) value. The compound tracing used GC-MS. The results showed that the simplicia and the extract of the leaves both natural-grown and cultivated contained secondary metabolites of alkaloids, flavonoids, steroids/triterpenoids, and tannins positively, whereas the glycosides were only found in cultivated type. The result of the antioxidant activity test showed that the ethanol extract of the leaves of A. beccariana van Tiegh both natural-grown and cultivated was categorized as very strong. The results of the identification of chemical compounds by GC-MS were identified 18 compounds in the natural-grown leaves, while 40 compounds were identified in the cultivated ones.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF PURNAJIWA FRUIT EXTRACT (Euchresta horsfieldii (Lesch.) Benn.)

2020 ◽  
Vol 8 (1) ◽  
pp. 31
Author(s):  
Romulia Trielva Apriliani ◽  
I Gede Putu Wirawan ◽  
Wayan Adiartayasa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Chemical Compound ◽  
Radical Reaction ◽  
Phytochemical Analysis ◽  
Free Radical Reaction ◽  
Fruit Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

Antioxidant is a chemical compound that could give one or more electrons to inhibit the free radical reaction. One of the plants that potentially produce antioxidants is purnajiwa (Euchresta horsfieldii (Lesch.) Benn.). The research was conducted using the fruit of purnajiwa. The purpose of this study is to know antioxidant activity from purnajiwa fruit using the DPPH method and measured by UV-Vis spectrophotometry. The extract of purnajiwa fruit gained by the maceration method using 96% ethanol solvent. The results of phytochemistry screening found out that the extract contains flavonoid, alkaloid, saponin, tannin, and terpenoid. According to flavonoid testing using quercetin, the results obtained from purnajiwa extract contained flavonoid of 1,651 mg QE/g. The activity test of antioxidants quantitatively by DPPH using UV-Vis spectrophotometry found out that purnajiwa extract has IC50 value of 57,28 ppm so that purnajiwa extract has strong antioxidant activity that could be used as natural antioxidant resources.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN MANGGA KASTURI (Mangifera casturi Kosterm.)

2021 ◽  
Vol 3 (3) ◽  
pp. 162-173
Author(s):  
Dwi Lestari ◽  
Muthia Dwi MA ◽  
Jati Pratiwi ◽  
Lidya Handoko Saputri
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

Mangga Kasturi (Mangifera casturi Kosterm.) is one of the plants in Indonesia that has potential as an antioxidant. The purpose of this study was to determine the IC50 value and antioxidant potential of the ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.). The ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.) was extracted using maceration with 96% ethanol as solvent. The antioxidant activity test was carried out using the DPPH (1,1-Diphenyl-2-Picrylhydrazil) method using a UV-Vis spectrophotometer and vitamin C as a comparison. The results of phytochemical screening tests on ethanol extracts showed positive secondary metabolites such as alkaloids, flavonoids, tannins, and quinones. Testing the ethanol extract of Mangga Kasturi leaves (Mangifera casturi Kosterm.) with the DPPH method showed that the ethanol extract had an antioxidant activity with an IC50 value of 83.61 ppm in the strong category of antioxidant potential.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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