scholarly journals Isolation, Purification and Characterization of Sericin Protein from the Discharge Water of Silk Industry

2020 ◽  
Vol 107 ◽  
pp. 1-6
Author(s):  

Sericin is a silk worm secreting protein occurring naturally as a silk cocoon.It is a typical example of a natural core-shell structure. Silk cocoon constitutes about 75% of core silk fibroin (SF) and 25% of shell silk sericin (SS). Silk fibroin has a potential application in the textile and biomedical field due to its inherent mechanical strength. Sericin is a hydrophilic protein that has a greater affinity with silk fibroin;hence it binds with silk fibroin coaxially to protect pupa from predators, temperature variations, moisture, mechanical abrasions, etc.. During the commercial production of raw silk fiber, sericin will be degummed by treating the cocoon with hot water at high temperatures and discharged as wastewater after extraction of silk fibroin from cocoons. Sericin protein posse’s potential application in the field of cosmetics as an additive in skin moisturizer, shampoo, anti-wrinkle creams due to the presence of hydroxyl amino acids, wound healing, promoting cell growth and differentiation in pharmaceutical industries due to its biological origin without causing an undesirable immunological response. Sericin protein was extracted from discharge water collected from the silk industry was purified and characterized using SDS-PAGE, FTIR, CD, SEM, DSC & TG-DTA was reported.

2012 ◽  
Vol 538-541 ◽  
pp. 52-59
Author(s):  
Jie Zhu ◽  
Ming Shi Li ◽  
Li Qun Wang ◽  
Xiao Lin Zhu

We reported the preparation of surface modified poly (ethylene oxide terephthalate) - poly (butylene terephthalate) membrane by the method of silk fibroin anchoring, namely SF/(PEOT/PBT). Its surface properties were characterized by contact angles and XPS and the biocompatibility of the composite membrane was further evaluated by human salivary epithelial cells (HSG cells) growth in vitro. Results revealed that SF/(PEOT/PBT) possessed the low water contact angle (48.0±3.0°) and immobilized a great amount of fibroin (fibroin surface coverage: 26.39 wt%), which attributed to the formation of polar groups such as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. HSG cells growth in vitro indicated that the silk fibroin anchoring could significantly enhance the biocompatibility of PEOT/PBT membrane, which suggested the potential application of fibroin anchoring PEOT/PBT for clinical HSG cells transplantation in the artificial salivary gland construct.


2020 ◽  
Author(s):  
Katie Miles ◽  
Bryn Hubbard ◽  
Duncan Quincey ◽  
Evan Miles ◽  
Ann Rowan

<p>Himalayan debris-covered glaciers contribute to the discharge of some of Earth’s largest river systems, shaping the seasonal water supply to millions of people. The supraglacial debris layer heavily influences the pattern of surface melt, producing a range of unique surface features that make it challenging to collect any data, particularly from the interior of such glaciers. Models of debris-covered glaciers therefore lack calibration and validation data, which are needed for accurate predictions of future glacier geometric change and contributions to river discharge, water resources and ultimately sea level. In 2017 and 2018, we logged four boreholes drilled using pressurised hot water into the debris-covered Khumbu Glacier, Nepal Himalaya, with a high-resolution optical televiewer. The boreholes were located at four sites across the lower glacier’s debris-covered area, down-flow of the Khumbu Icefall. The resulting logs, ranging in length from 22–150 m, produced a 360° geometrically-accurate full-colour image of each borehole at ~1 mm vertical and ~0.22 mm (1,440 pixel) horizontal resolution. The logs reveal three material facies: i) steeply-dipping ice layers, some including debris; ii) steeply-dipping sediment-rich layers; and iii) clusters of sediment and debris dispersed through the ice. On the basis of these facies, we present reconstructions of the glacier’s structure and historical flow paths and the first measurements of the englacial debris concentration of a Himalayan debris-covered glacier. From the latter, we additionally infer both the sources of this englacial debris and of the supraglacial debris layer present across much of the lower ablation area of Khumbu Glacier.</p>


2013 ◽  
Vol 796 ◽  
pp. 428-431
Author(s):  
Wei Guo Chen ◽  
Zong Qian Wang ◽  
Zhi Hua Cui ◽  
Zhao Cheng Meng

High wet fastness dyeing is always the hot issue of silk industry. The plenty of tyrosine residue in silk protein may contribute an opportunity to solve this problem. In this paper, in situ coloration of silk is described using coupling reaction between diazonium compounds, made from different arylamine derivatives, and tyrosine residues in the silk fibroin. The spectra of coloured silk and the washing and rubbing fastness were measured. The results suggest that the use of coupling reactions could be developed into a dyeing method for silk. The wet fastness properties of such coloration are good because the chromophores are incorporated into the protein chains.


2005 ◽  
Vol 2005 ◽  
pp. 160-160
Author(s):  
Z. Ansari Pirsaraii ◽  
B. Navidshad

Silkworm pupae meal is a silk industry by-product that in some countries is available as a local product. It contain up to 30% crude fat and 50% crude protein. The chemical score of silkworm pupae protein is 60 and tryptophan is its limiting amino acid. The silk worm pupae meal fat contains 25.7% linoleic acid (Udayasekhara Rao, 1994). Reddy et al (1991) reported that use of diets contain 5% 8% SWP so that replaced 50% of fish meal, lowered weight gain. The aim of this study was to measure the effects of substituting different levels of silkworm pupae (SWP) for soybean meal (SBM) in Arian (an Iranian strain) broiler rations.


2020 ◽  
Vol 172 ◽  
pp. 12003
Author(s):  
Nikola Pokorny ◽  
Tomas Matuska

The paper deals with performance analysis of potential application of glazed photovoltaic-thermal collector for domestic hot water preparation for multifamily building in European climatic conditions. Two different solutions are studied, glazed photovoltaic-thermal collectors integrated in the building envelope and glazed photovoltaic-thermal collectors fixed on the roof of the building. Moreover, the paper presents a comparison with conventional side by side installation of solar thermal collectors and photovoltaic panels to show the benefit of photovoltaic-thermal collectors. Simulation analysis has been done in TRNSYS with use of developed and validated mathematical model of glazed photovoltaic-thermal collector.


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