scholarly journals Comparative effectiveness of eimeriocidal products for treatment of broiler chickens in small scale production

2021 ◽  
pp. 295-300
Author(s):  
A. B. Dagaeva ◽  
B. M. Makhieva
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Broiler Chickens ◽  
Clinical Signs ◽  
Small Scale ◽  
Control Group ◽  
Scale Production ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

There is currently almost no poultry holding where avian eimerioses, both monoinvasions and those associated with cryptosporidioses, salmonelloses and colibacte- rioses, are not reported. In view of this, the disease control is an urgent challenge that shall be approached in its entirety, using various eimeriostats, antibiotics and probiotics. Searching for new effective products with broad-spectrum antiparasitic action is one of priorities in avian eimeriosis control. Comparative tests of different combinations of eimeriocidal products, namely solicox + chicktonic, maduvet + tylosin and eimeterm + enrofloxacin, for their treatment and protective effectiveness were carried out under production conditions in broiler chickens of a poultry factory located in the Republic of Dagestan. To perform the tests, four groups of broiler chickens (one control group and three test groups, each comprising 50 chickens) were formed based on the principle of analogues. The treatment and prevention scheme adopted in the said poultry holding was used for the control group chickens. Test group 1 chickens were given solicox at a dose of 2 ml per 1 liter of drinking water in combination with chicktonic (a feed supplement) at a dose of 1 ml per 1 liter of water during 4–5 days. Group 2 chickens were given maduvet at a dose of 3−5 mg/kg of body weight with feed twice and tylosin at a dose of 5 g of powder per 10 liters of water once a day during 5 days; where necessary, the procedure was repeated in 14–16 days. Group 3 broiler chickens were given eimeterm 2.5% at a dose of 7 mg/kg of body weight with water during 2 days in combination with enrofloxacin at a dose of 3 ml per 1 liter of drinking water during 5–6 days. It is shown that a decrease in the number of clinically diseased and dead chickens was observed in the test groups after the use of eimeriocidal products that helped to improve zootechnical parameters of reared broiler chicks. Product extenseffectiveness and intens- effectiveness in different groups of chickens were as follows: Group 1 – 89.2 and 96%, Group 2 – 87.6 and 94%, Group 3 –81.4 and 96%, respectively. The clinical signs of eimeriosis were observed in the control group chickens throughout the entire period of studies, invasion extensity and intensity were 87.6 and 42.6%, respectively.

scholarly journals The Effect of Licorice roots powder on experimental Infection of infectious bursal disease in broiler chickens

2006 ◽  
Vol 30 (2) ◽  
pp. 65-78
Author(s):  
A. K. Kassab
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Food Intake ◽  
Water Content ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Age Group ◽  
Bursal Disease ◽  
Group 3 ◽  
Group 1

This study was conducted to investigate the effect of Licorice roots powder for 35 days in reducing the severity of infection with infectious bursal disease (IBD)and the immune response and performance of broiler chicks .A Total of 150 day old broiler chickens were divided into 5 groups and treated as follows : Group 1: infected with IBD virus at 21 days of age. Group 2: infected with IBD virus at 21 days of age and given 1 gm of Licorice roots powder /1 L of drinking water one day after infection . Group 3 : given 1 gm Licorice roots powder / 1L of drinking water at day one of age and infected with IBD virus at day 21 of age . Group 4:given 1 gm Licorice roots / 1 L of drinking water at 22 days of age .Group 5: untreated control. Antibody (Ab) of IBD titer was measured by ELIZA , the serum total protein (STP) the serum Globulin(SG)and the blood picture were determined , the water content of the droppings was measured , the body weight , food intake was recorded, there was increase (P<0.01) in the (Ab)titer of IBD virus in the group3 comparing with the other groups , the bursa / body weight index (B:BW)three days after infection was increased (P<0.01) in group1 , the STP , the SG , the PCV and RBCs was increased (P<0.01) in group 3 at age 27 days the food intake (FI) Food conversion ratio (FCR) and body weight (BW) in Group 3 were significantly increased , the dropping water content was significantly (P<0 .01)increased in group 3 in comparison with other groups at the period from 23-27 days of age, the morbidity and Mortality rate were significantly lower in group 3 and then 2 in comparison with group 1 (50 % , 63.3 %, 90 % ) and (6.6 % , 16.6 % ,30%) respectively

scholarly journals The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

2018 ◽  
Vol 87 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Mehtap Ozcelik ◽  
Mine Erişir ◽  
Osman Guler ◽  
Murat Baykara ◽  
Esra Kirman
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Sod Activity ◽  
Irradiation Group ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The effect of irradiation on oxidants and antioxidants in selected tissues and a possible protective effect of curcumin on these indices were investigated. A total of 28 rats were divided into 3 groups; group 1 was control; group 2 was the irradiation group, saline was administered intraperiotenally (i.p.) for three days and then, 9 Gy gamma irradiation was applied; group 3 was the irradiation + curcumin group: curcumin was given i.p. for three days at 200 mg/kg body weight and then the same dose of irradiation was applied. A significant increase in malondialdehyde (MDA) was detected in the liver, kidney, and brain tissues of the rats as a result of irradiation (P< 0.01). Glutathione peroxidase (GSH-Px) activity in all the tissues (except for kidneys) decreased (P< 0.01), liver SOD (superoxide dismutase) activity decreased (P< 0.05), and GSH (glutathione) levels in kidney and ovary tissues (P< 0.001) significantly increased. While curcumin administration returned the increased MDA levels in the kidneys and brain in result of irradiation to normal (P< 0.01), it did not return the increased MDA levels in the liver tissue to normal (P< 0.001) despite significantly reducing them. While decreased GSH-Px and SOD activity in the liver in result of irradiation increased with the addition of curcumin (P< 0.05), increased GSH levels in the kidneys and ovaries returned to control levels (P< 0.001). When MDA values were examined, it was found that the addition of curcumin protected the liver, kidneys and brain from the oxidative damage caused by irradiation.

scholarly journals HISTOLOGICAL STRUCTURE OF PROXIMAL METAPHYSEAL CARTILAGE OF HUMERUS IN RATS AFTER 60-DAY TARTRAZINE ADMINISTRATION

2020 ◽  
pp. 150-157
Author(s):  
V.I. Luzin ◽  
O.N. Fastova ◽  
V.N. Morozov ◽  
E.N. Morozova ◽  
S.V. Zabolotnaya
Keyword(s):  
Body Weight ◽  
Male Rats ◽  
Control Group ◽  
Histological Structure ◽  
Total Width ◽  
Adult Rats ◽  
Study Results ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The aim of the research is to study the histological structure of proximal metaphysial cartilage of the humerus in adult rats after 60 days of tartrazine administration (750 mg/kg and 1500 mg/kg body weight). Materials and Methods. The study was carried out on 90 white male rats, divided into 3 groups. In Group 1 (control), animals daily intragastrically received 1ml of 0.9 % isotonic sodium chloride solution for 60 days. Rats of Groups 2 and Group 3 received intragastrically 1ml of tartrazine solution (750 mg/kg and 1500 mg/kg body weight respectively) for 60 days. The readaptation period after the last tartrazine administration was 3, 10, 15, 24, or 45 days. The area of the proximal humerus metaphysis underwent treatment according to the standard histological protocol. The obtained sections were stained with hematoxylin-eosin and subjected to a subsequent histomorphometric study. Results. On the 3rd day of the readaptation period, the total width of the proximal metaphysial cartilage of the humerus in animals of Group 2, was by 8.59 % less than the values of the control group; the width of the indifferent, proliferating and definitive cartilage was by 6.11 %, 8.85 % and 7.15 % less; the width of the destruction zone was by 11.55 % less; and the width of the osteogenesis zone was by 9.92 % less. The content of primary spongiosis in the osteogenesis zone and the number of cells on the trabeculae surface were lower than the values of Group 1 by 8.45 % and 8.42 % respectively. During the readaptation period, the authors observed similar dynamics of changes in the histomorphometric parameters of the metaphysical cartilage of the humerus with levelling by the 45th day of the experiment. In rats of Group 3, during the entire readaptation period, the histomorphometric parameters changed more significantly and their recovery to control values was slower than in animals of Group 2. Conclusion. 60-day tartrazine administration to adult rats is accompanied by inhibition of the bone formation function of the proximal metaphysial cartilage of the humerus. It is confirmed by a decrease in the width of all its zones and components of the osteogenesis zone. More pronounced in amplitude and duration changes in the parameters studied were recorded in animals treated with tartrazine, 1500 mg/kg body weight. Keywords: humerus, metaphysial cartilage, tartrazine, histomorphometry. Цель исследования. Установить, оказывает ли влияние 60-дневное введение тартразина в дозах 750 и 1500 мг/кг массы тела на гистологическое строение проксимального метафизарного хряща плечевой кости у половозрелых крыс. Материалы и методы. Исследование проведено на 90 белых крысах-самцах, распределенных на 3 группы: 1-я – контрольная, в которой животные ежедневно в течение 60 дней получали физиологический раствор через желудочный зонд; 2-ю и 3-ю группы составили крысы, которые на протяжении 60 дней получали внутрижелудочно 1 мл раствора тартразина в дозах 750 и 1500 мг/кг массы тела соответственно. Сроки периода реадаптации после окончания введения тартразина составляли 3, 10, 15, 24, 45 сут. Проксимальный метафиз плечевой кости подвергался обработке по стандартному гистологическому протоколу. Полученные срезы окрашивались гематоксилин-эозином и подвергались последующему гистоморфометрическому исследованию. Результаты. У животных 2-й группы на 3-й день периода реадаптации общая ширина проксимального метафизарного хряща плечевой кости была меньше значений контрольной группы на 8,59 %, ширина зон индифферентного, пролиферирующего и дефинитивного хряща – на 6,11, 8,85 и 7,15 % меньше, ширина зоны деструкции – на 11,55 %, а ширина зоны остеогенеза – на 9,92 %. Содержание первичной спонгиозы в зоне остеогенеза и количество клеток на поверхности трабекул были меньше значений контрольной группы на 8,45 и 8,42 %. В ходе периода реадаптации сходная динамика изменений гистоморфометрических параметров метафизарного хряща плечевой кости сохранялась с тенденцией к сглаживанию к 45-м сут эксперимента. У крыс 3-й группы в ходе всего периода реадаптации изучаемые параметры изменялись более значимо и их восстановление до контрольных величин происходило медленнее, чем во 2-й группе. Выводы. 60-дневное введение тартразина половозрелым крысам сопровождается угнетением костеобразовательной функции проксимального метафизарного хряща плечевой кости, что подтверждается снижением ширины всех его зон и объемных компонентов зоны остеогенеза. Более выраженные по амплитуде и длительности изменения изучаемых параметров зафиксированы у животных, получавших тартразин в дозе 1500 мг/кг массы тела. Ключевые слова: плечевая кость, метафизарный хрящ, тартразин, гистоморфометрия.

scholarly journals The Effect of Inorganic or Organic Zinc on the Morphology of the Intestine in Broiler Chickens

2017 ◽  
Vol 61 (3) ◽  
pp. 52-56 ◽  
Author(s):  
M. Levkut ◽  
M. Fukasová ◽  
K. Bobíková ◽  
M. Levkutová ◽  
K. Čobanová ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Basal Diet ◽  
Control Group ◽  
Villus Height ◽  
Organic Zinc ◽  
Group 4 ◽  
Diet Control ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

AbstractThis study compared the effect of dietary supplementation with an inorganic or organic zinc source on the gut morphology in the jejunum of broilers. One-day-old chickens were fed a basal diet (Control group: BD — 32 mg Zn.kg−1DM), or the same BD supplemented with 30 mg or 70 mg of Zn per kg of DM in the form of ZnSO4.H2O (Group 1: 30 mg ZnSO4; Group 2: 70 mg ZnSO4), and 30 mg or 70 mg of Zn per kg of DM in the form of zinc chelate of glycine hydrate (Group 3: 30 mg Zn-Gly; Group 4: 70 mg Zn-Gly) for 40 days. The villus height was increased in the groups which received 30 mg ZnSO4and 70 mg ZnSO4and or 70 mg ZnSO4, as compared to the BD and 30 mg Zn-Gly. The villus surface was higher in all groups receiving the Zn supplements in comparison to the BD.

scholarly journals FULVIC ACID PREPARATION INFLUENCE ON BROILER CHICKEN GROWTH RATE

2020 ◽  
Author(s):  
Ю.В. АРЖАНКОВА ◽  
Т.И. Скопцова ◽  
С.А. Попова
Keyword(s):  
Drinking Water ◽  
Fulvic Acid ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Live Weight ◽  
Optimum Dose ◽  
Control Group ◽  
Group 3 ◽  
Group 2 ◽  
Experimental Group

Целью исследований явилось изучение живой массы и среднесуточных привесов цыплят-бройлеров кросса «Hubbard» при введении в питьевую воду препарата фульвиокислоты компании BioSap Technologies. Птицы контрольной и опытных групп получали в период выращивания одинаковый рацион (полнорационные комбикорма престарт, старт-1, старт-2, рост, финиш) в соответствии с возрастом. Птицы трех опытных групп дополнительно получали в питьевой воде 2, 4 и 6% препарата фульвиокислоты, произведенного из сапропеля озера Жарки Тверской области. К моменту забоя в 40-дневном возрасте наилучший результат по живой массе – 2592,97 г – получен в опытной группе 2 (4% препарата), птицы которой достоверно превосходили бройлеров опытной группы 3 (6% препарата) на 203,31 г или на 8,51% (p<0,01). У курочек опытной группы 2 показатель составил 2475,53 г, что достоверно больше по сравнению с курочками опытной группы 3 на 245,53 г, или на 11,01% (p<0,01). Цыплята-бройлеры опытных групп 1 и 2 превосходили по живой массе птиц контрольной группы, у петушков это характерно и для опытной группы 3. Наилучшие результаты по среднесуточному приросту отмечены также в опытной группе 2. Без учета пола результат составил 63,82 г, что достоверно больше по сравнению с показателем опытной группы 3 на 5,05 г, или на 8,59% (p<0,05). Среднесуточный прирост курочек опытной группы 2 равен 60,88 г, что на 6,09 г или на 11,12% больше по сравнению с курочками опытной группы 3 (p<0,01). Цыплята-бройлеры опытной группы 3 без учета пола, а также курочки уступают по этому показателю птицам контрольной группы. Оптимальной дозой дачи препарата BioSap Fulvic Power компании BioSap Technologies цыплятам-бройлерам следует считать добавление 4% в питьевую воду. The research goal was to learn how the live weight and average daily weight gains of «Hubbard» cross broiler chickens change when their drinking water is added with a fulvic acid preparation produced by the BioSap Technologies company. Birds of both the control and experimental groups were fed in the raising period the similar feeding rations (complete-value combined feeds of pre-start, start-1, start-2, growth, finishing) depending on the growth stage of chickens. The birds in the experimental groups were fed additionally 2, 4 и 6% fulvic acid preparations produced from the sapropel of the Tver oblast Zharki lake .By the slaughter time, at the age of 40 days, the best live weight results of 2592,97 grammes were shown by experimental group 2 fed with a 4% preparation, which reliably exceeded the weight of group 3 chickens (6% preparation) by 203,31 grammes, or by 8,51% (p<0,01). With female chickens in group 2 the value was 2475,53 grammes, which reliably exceeded the weight of group 3 chickens by 245,53 grammes, or by 11,01% (p<0,01) Broiler chickens of experimental groups 1 and 2 exceeded the control group, the same was noted with male chickens ot experimental group 2 too. The best results in average daily gain were also recorded in experimental group 2. Sex differences not being considered, the result was 63,82 grammes, which reliably exceeded the weight of group 3 chickens by 5,05 grammes, or by 8,59% (p<0,05). Daily gains of female chickens in experimental group 2 were 60,88 grammes, or 6,09 grammes higher than those in experimental group 3 (11,12%). Broiler-chicken values of experimental group 3, sex not being considered, as well as female chicken gains were lower than those of the control group. The optimum dose of the BioSap Fulvic Power preparation produced by the BioSap Technologies company should be considered that of 4% added into drinking water.

scholarly journals Biochemical Changes in the Malnourished Rats Serum and Liver Exposed to Dietary Monosodium Glutamate

2019 ◽  
pp. 156-167
Author(s):  
Babawande A. Origbemisoye ◽  
Badiu A. Akinbode ◽  
Ganiyat A. Oparemi
Keyword(s):  
Body Weight ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
Weight Group ◽  
Stress Biomarkers ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Monosodium glutamate (MSG) is a flavor enhancer. Its toxicity in a malnourished state appears not to have been fully investigated. This study was carried out to determine the effects of MSG on malnourished rats. Rats were randomly assigned into four groups of five rats/group. Group 1 rats were fed with malnourished feed; Group 2 rats received malnourished feed with dosed 1.6 mg/g MSG per body weight; Group 3 rats were fed with normal feed and dosed 1.6 mg/g MSG per body weight and Group 4 rats served as the control group (normal healthy rats) and were fed with normal feed for 28 days. After 28 days, the rats were sacrificed with the liver harvested and blood samples collected. Results from the study showed that malnourished rats had significantly lower levels of oxidative stress biomarkers including, anti-oxidants compared with the control. The levels of malondialldehyde concentration and xanthine oxidase activity were high in malnourished fed rats. Aspartate aminotransferase and alanine transaminase levels of malnourished and normal rats administered MSG were significantly low compared to the normal healthy suggesting that labialization occurs in liver leading to leakage of these enzymes from the liver to the serum. Malnourished rats showed significant decrease in body weight losing 48 grams after 28 days compared to malnourished and normal rats fed with MSG which recorded significant increase in body weight after 28 days adding 26 g and 42 g respectively.

scholarly journals Neonatal mortality associated with sodium monofluoracetate in kids fed with colostrum from goats ingesting Amorimia septentrionalis

2019 ◽  
Vol 39 (3) ◽  
pp. 163-167 ◽  
Author(s):  
José R.G. Lopes ◽  
José A.S. Araújo ◽  
Danielle A.N. Pessoa ◽  
Stephen Lee ◽  
Daniel Cook ◽  
...  
Keyword(s):  
Body Weight ◽  
Arid Region ◽  
Clinical Signs ◽  
The Other ◽  
Jugular Veins ◽  
Group 3 ◽  
Group 2 ◽  
Semi Arid Region ◽  
Semi Arid ◽  
Group 1

ABSTRACT: Sudden deaths after colostrum ingestion in kids and lambs born to mothers grazing in areas with Amorimia septentrionalis have been reported in the Brazilian northeastern semi-arid region, in Paraíba state. This study aimed to determine whether the sodium monofluoracetate (MF) contained in A. septentrionalis is eliminated in milk, causing the death of kids. After confirming gestation on the 25th day after mating, 26 goats were randomly distributed into three groups. In Group 1, eight goats received fresh leaves of A. septentrionalis in daily doses of 1g/kg body weight, administered at three different periods during gestation: from days 91 to 100, 116 to 125, and from day 140 until delivery day. In Group 2, consisting of 10 females, eight goats received 1g/kg body weight of A. septentrionalis dried and milled leaves, fed daily from the 140th day of gestation until delivery. The other two goats of this group did not ingest the plant during gestation and after delivery the colostrum supplied to their kids was replaced by colostrum of goats from that same group that had ingested the plant. Eight goats from Group 3 (control) did not ingest A. septentrionalis. Seven goats from Group 1 showed signs of poisoning from 2nd to 8th days of plant administration, in all periods, and recovered within 7 to 12 days. Another goat presented severe clinical signs and was submitted to euthanasia in extremis. Two goats aborted. Four kids, from two goats, received colostrum and, after 15 minutes, presented depression, breathing wheezing, lateral recumbence, bleating, and death. Two goats gave birth at night; the two kids were found dead and, at necropsy, it was verified that they were born alive. The last goat in this group gave birth to two kids which showed no signs of poisoning after colostrum ingestion. In Group 2, the eight goats that ingested dry leaves of the plant presented tachycardia and engorgement of the jugular veins; six aborted, and the kids of the other two goats died immediately after delivery without ingesting colostrum. The three kids of the two goats that did not ingest the plant during gestation did not show signs of poisoning after ingesting colostrum from the goats that had ingested the plant. In Group 3, all females kidded normally and the kids showed no signs of poisoning. Ten leaf samples of A. septentrionalis contained 0.00074% ±0.00018 MF. These results demonstrate that the MF of A. septentrionalis is eliminated in colostrum and may cause the death of kids. As in previous reports, the plant also caused abortion.

Postoperative Morphine Use and Hyperalgesia Are Reduced by Preoperative but Not Intraoperative Epidural Analgesia

2003 ◽  
Vol 98 (6) ◽  
pp. 1449-1460 ◽  
Author(s):  
Joel Katz ◽  
Lorenzo Cohen ◽  
Roger Schmid ◽  
Vincent W.S. Chan ◽  
Adarose Wowk
Keyword(s):  
Morphine Consumption ◽  
Gynecologic Surgery ◽  
Control Group ◽  
Epidural Administration ◽  
Blinded Manner ◽  
Group 3 ◽  
Group 2 ◽  
Double Blinded ◽  
Postoperative Morphine ◽  
Group 1

Background The aim of this study was to evaluate the postoperative morphine-sparing effects and reduction in pain and secondary mechanical hyperalgesia after preincisional or postincisional epidural administration of a local anesthetic and an opioid compared with a sham epidural control. Methods Patients undergoing major gynecologic surgery by laparotomy were randomly assigned to three groups and studied in a double-blinded manner. Group 1 received epidural lidocaine and fentanyl before incision and epidural saline 40 min after incision. Group 2 received epidural saline before incision and epidural lidocaine and fentanyl 40 min after incision. Group 3 received a sham epidural control (with saline injected into a catheter taped to the back) before and 40 min after incision. All patients underwent surgery with general anesthesia. Results One hundred forty-one patients completed the study (group 1, n = 45; group 2, n = 49; group 3, n = 47). Cumulative patient-controlled analgesia morphine consumption at 48 h was significantly lower (P = 0.04) in group 1 (89.8 +/- 43.3 mg) than group 3 (112.5 +/- 71.5 mg) but not group 2 (95.4 +/- 60.2 mg), although the hourly rate of morphine consumption between 24 and 48 h after surgery was significantly lower (P &lt; 0.0009) in group 1 (1.25 +/- 0.02 mg/h) than group 2 (1.41 +/- 0.02 mg/h). Twenty-four hours after surgery, the visual analog scale pain score on movement was significantly less intense (P = 0.005) in group 1 (4.9 +/- 2.2 cm) than group 3 (6.0 +/- 2.6 cm) but not group 2 (5.3 +/- 2.5 cm), and the von Frey pain threshold near the wound was significantly higher (P = 0.03) in group 1 (6.4 +/- 0.6 log mg) than in group 3 (6.1 +/- 0.8 log mg) but not group 2 (6.2 +/- 0.7 log mg). Conclusions Preincisional administration of epidural lidocaine and fentanyl was associated with a significantly lower rate of morphine use, lower cumulative morphine consumption, and reduced hyperalgesia compared with a sham epidural condition. These results highlight the importance of including a standard treatment control group to avoid the problems of interpretation that arise when two-group studies of preemptive analgesia (preincisional vs. postsurgery) fail to find the anticipated effects.

scholarly journals Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lyudmil Terziev ◽  
Violeta Dancheva ◽  
Veneta Shopova ◽  
Galya Stavreva
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Mice Model ◽  
Antioxidant Defence ◽  
Group 4 ◽  
Antioxidant Defence System ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

scholarly journals Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir
Keyword(s):  
Mus Musculus ◽  
Ovarian Follicles ◽  
Observation Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

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