scholarly journals Comparison of Chromogenic Culture Media, Rapid Immunochromatographic Test and Temocillin Resistance for The Detection of OXA-48 Carbapenemase-Positive Klebsiella Pneumonia Strains

2021 ◽  
Vol 12 (4) ◽  
pp. em00778
Author(s):  
Erkan Sanmak ◽  
Sebahat Aksaray
Keyword(s):  
Culture Media ◽  
Klebsiella Pneumonia ◽  
Immunochromatographic Test

scholarly journals Bacterial Profile and Antibiogram of Hospital-Acquired Pneumonia and Ventilator-Associated Pneumonia Patients in ICU of Raden Mattaher Hospital Jambi

2020 ◽  
Vol 26 (3) ◽  
Author(s):  
Sotianingsih Sotianingsih ◽  
Samsirun H. ◽  
Lipinwati Lipinwati
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Culture Media ◽  
Sensitivity Test ◽  
Sputum Culture ◽  
Klebsiella Pneumonia ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Acinetobacter Baumanii ◽  
Gram Negative ◽  
Gram Positive Bacteria

Pneumonia is defined as an inflammation of the lungs caused by microorganisms (bacteria, viruses, fungi, parasites). This research aimed to determine the pneumonia-causing bacteria along with the sensitivity and the antibiotic resistance test. This research was a descriptive study with samples of ICU pneumonia patients at Raden Mattaher Regional Hospital during the study period. All samples were consecutively selected. Samples for blood culture were incubated in the BactAlert device, whereas the sensitivity test was then performed using Vitex instruments. Sputum was previously enriched with BHI media and then cultured on culture media, and sensitivity test with the Vitex instruments was carried out. Of the 354 ICU patients during the study period, 30 patients (11.8%) had pneumonia, but only 19 patients could undergo sputum culture. Five of 19 patients were infected with Gram-positive bacteria, and 14 patients were infected with Gram-negative bacteria. The most commonly found bacteria were Klebsiella pneumonia (36.84%), followed by Acinetobacter baumanii (21.05%) and Pseudomonas aeruginosa (10.53%). Gram-negative bacteria obtained from sputum culture in this study were resistant to almost all antibiotic groups, especially penicillin, cephalosporin, quinolone, and tetracycline groups. Gram-positive bacteria obtained from sputum culture in this study were resistant to the penicillin antibiotic. The most commonly found bacteria were Klebsiella pneumonia (36.84%), followed by Acinetobacter baumanii (21.05%) and Pseudomonas aeruginosa (10.53%). The bacteria cultured from the sputum showed multidrug resistance mainly to the penicillin and cephalosporin antibiotic. This research data can be used to consider the treatment of pneumonia patients to decide more appropriate therapy.

scholarly journals Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

2021 ◽  
Vol 28 (11) ◽  
pp. 1571-1577
Author(s):  
Sajid Ali ◽  
Hina Khalid ◽  
Noor Muhammad ◽  
Syed Luqman Shuaib ◽  
Anees Muhammad
Keyword(s):  
Culture Media ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Antibiotics Resistance ◽  
Klebsiella Pneumonia ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
E Coli ◽  
Antibiotic Resistance Profile

Objectives: The present study is aimed to determine frequency of bacteria in urine and pus sample along with the antibiotic resistance profile of isolated bacteria. Study Design: Cross-Sectional Study. Setting: Real-Time PCR Laboratory at Dabgari Garden, Peshawar with the Collaboration of Health Biotechnology Laboratory, Department of Biotechnology, Abdul Wali Khan University, Mardan. Period: June 2018 to July 2019. Material & Methods: The urine and pus samples were collected from suspected patients and were cultured on appropriate culture media. The biochemical tests were also performed after growing on culture plates for identification and confirmation of bacterial isolates. The disc diffusion technique was used to evaluate the antibiotic pattern of retrieved isolates. Result: A total of 525 samples of pus and urine samples were collected from different regions of Peshawar. Out of the total, 237 (45.1%) samples were found positive for bacterial growth whereas the remaining (54.9%) were observed negative. Among total positive isolates, 220 (92.8%) were from urine samples, and 17 (7.2%) were from pus samples. The predominant isolate was Escherichia coli (E. coli) (90.3%) retrieved from positive samples, followed by Pseudomonas aeruginosa (7.2%), Klebsiella pneumonia (1.3%), and Staphylococcus aureus (1.3%). The most effective antibiotic was Fosfomycin against bacteria whereas Nalidixic acid, Nitrofurantoin, and Amoxicillin/clavulanic acid were found less effective against bacterial isolates. Conclusion: Most frequent bacteria isolated was E. coli and the most efficient drug was Fosmomycin and the least was Nitrofurantoin, and Amoxicillin.

scholarly journals Isolation and Identification of Aerobic Bacterial Pathogens from Blood Culture of Cancer Patients in Al-Zara Hospital in Khartoum, Sudan

2021 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Areeg Abd-Alaziz Alnoor Mohammed ◽  
◽  
Babbiker Mohammed Taher Gorish ◽  
Keyword(s):  
Blood Culture ◽  
Cancer Patients ◽  
Culture Media ◽  
Multidrug Resistant ◽  
Klebsiella Pneumonia ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Patients With Cancer ◽  
Antimicrobial Sensitivity ◽  
Study Results

Patients with cancer are particularly under risk of many microbial infections and even septicaemia due to the weakened immune system induced by Chemotherapy. This study was done to identify and isolate aerobic bacterial septicaemic pathogens among cancer patients. This study was performed in Radiation and Isotopes national Centre of Khartoum Hospital (previously Alzarra) during the period from September to November 2019. One Hundred Fifty blood samples were collected from cancer patients suspected to have septicemia. All samples where inculated in blood culture media and incubated aerobically. Upon detection of growth signs the bacterial isolates were subcultured and identified according to the Standard known procedure. Antimicrobial sensitivity test was done following CLSI guidelines, The study showed that Twenty two (14%) of the investigated samples were showed growth signs, while One hundred Twenty eight (86%) were showed no growth. After subculture on Blood agar, MacConky agar and Chocolate agar, all isolated pathogens were subjected to essential bacteriological biochemical tests and identified as E.coli (57.1%) , Klebsiella pneumonia (19%) ,Staphylococcus aureus( 9.5%), Pseudomonas aeruginosa (4.8%) . Citrobacter Spp (4.8%) and Streptococcus pyogens (4.8%). Septicaemia in patients with cancer was mainly caused by E.coli in patients using chemotherapy. Further study with inclusion of more sample size and focusing on multidrug resistant isolates is essential to verify the current study results.

scholarly journals A Identification and Isolation of Several Bacteria from oral cavity among human in Soran City

2021 ◽  
Vol 26 (5) ◽  
pp. 1-7
Author(s):  
Holem Balaky ◽  
Alaa Taha Younis Al-Hammadi
Keyword(s):  
Susceptibility Testing ◽  
Culture Media ◽  
Enterobacter Aerogenes ◽  
Biochemical Properties ◽  
Oral Bacteria ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Different Types ◽  
The City ◽  
Number Of Bacteria

The aim of this study has been to estimate that the number of bacteria in the mouth exceeds the total number of people on the planet. In this context, scientists have discovered more than 700 different types of human oral bacteria. With such a large number of oral bacteria present, many diseases that may threaten health, especially oral health, can emerge. For this reason, this study was employed and conducted on 252 healthy people in the Soran area of the city of Erbil. Both sexes were involved and different ages were selected. Swabs taken from 226 participants gave positive growths on different culture media. The biochemical properties of the isolates were tested according to Bergeys Manual of Systematic Bacteriology and UK Standards for Microbiology Investigation. The results showed that Staphylococcus aureus was the most frequent isolated pathogen (47.83%) followed by Pseudomonas aeruginosa (16.30%), Bacillus subtilis (23.91%), Enterococcus faecalis (5.43%), Enterobacter aerogenes (4.35%), and Klebsiella pneumonia (2.17%) respectively. Antibiotic Susceptibility testing of different species of bacteria was also performed according to Kirby Bauer disc diffusion method on Muller Hinton Agar by using commercial antibiotic discs. It seems that the different species of bacteria showed various sensitivity patterns to several kinds of antibiotics.

scholarly journals Isolation and Identification of Aerobic Bacterial Pathogens from Blood Culture of Cancer Patients in Al-Zara Hospital in Khartoum, Sudan

2019 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Areeg Abd-Alaziz Alnoor Mohammed ◽  
◽  
Babbiker Mohammed Taher Gorish ◽  
Keyword(s):  
Blood Culture ◽  
Cancer Patients ◽  
Culture Media ◽  
Multidrug Resistant ◽  
Klebsiella Pneumonia ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Patients With Cancer ◽  
Antimicrobial Sensitivity ◽  
Study Results

Patients with cancer are particularly under risk of many microbial infections and even septicaemia due to the weakened immune system induced by Chemotherapy. This study was done to identify and isolate aerobic bacterial septicaemic pathogens among cancer patients. This study was performed in Radiation and Isotopes national Centre of Khartoum Hospital (previously Alzarra) during the period from September to November 2019. One Hundred Fifty blood samples were collected from cancer patients suspected to have septicemia. All samples where inculated in blood culture media and incubated aerobically. Upon detection of growth signs the bacterial isolates were subcultured and identified according to the Standard known procedure. Antimicrobial sensitivity test was done following CLSI guidelines, The study showed that Twenty two (14%) of the investigated samples were showed growth signs, while One hundred Twenty eight (86%) were showed no growth. After subculture on Blood agar, MacConky agar and Chocolate agar, all isolated pathogens were subjected to essential bacteriological biochemical tests and identified as E.coli (57.1%) , Klebsiella pneumonia (19%) ,Staphylococcus aureus( 9.5%), Pseudomonas aeruginosa (4.8%) . Citrobacter Spp (4.8%) and Streptococcus pyogens (4.8%). Septicaemia in patients with cancer was mainly caused by E.coli in patients using chemotherapy. Further study with inclusion of more sample size and focusing on multidrug resistant isolates is essential to verify the current study results.

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

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