Chromogenic culture media or rapid immunochromatographic test: Which is better for detecting Klebsiella pneumoniae that produce OXA-48 and can they be used in blood and urine specimens

2018 ◽  
Vol 148 ◽  
pp. 169-173 ◽  
Author(s):  
Ozlem Genc ◽  
Evrim Aksu
Keyword(s):  
Klebsiella Pneumoniae ◽  
Culture Media ◽  
Immunochromatographic Test ◽  
Urine Specimens

scholarly journals Prevalence of SUL(1,2), GYR(A, B) and OXA genes among multidrug resistance Klebsiella pneumoniae isolates recovered from women suffering urinary tract infection

2020 ◽  
Vol 11 (2) ◽  
pp. 2424-2432
Author(s):  
Nabil Salim Saaid Tuwaij ◽  
Huda Jameel Baker Al-khilkhali ◽  
Haneen Mohamed Mohsen
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Vitek 2 ◽  
Resistant Microorganism ◽  
Urine Specimens

Klebsiella pneumoniae is a significant concern multidrug-resistant microorganism and a one common gram negative bacteria associated with infections of women urinary tract. Therefore, this work aimed to the molecular screening of Sul(1and 2), Gyr(A and B) and OXA genes among K. pneumoniae isolates in Najaf City, Iraq. Out of 250 urine specimens were collected from women showing symptoms of urinary tract infection during five months January to of May 2019, bacterial growth was157 isolates, included 133 gram negative compared with  24 gram positive bacteria while 98 specimens were no growth. According to the Vitek-2 system, 30 K. pneumoniae isolates were obtained.Data on current work revealed that the 26-35 age group was the highest 14 K. pneumoniae isolates. Results of antimicrobial susceptible recorded all isolates were multi-drug resistant (MDR) and they have a different range of resistance. However, all 30 isolates(100%) resistant to ampicillin drugs, while the lowest rate was 1(3.33%) forImipenemdrug. PCR assay revealed exist of oxa, sul-1, sul-2, gyr-A and gyr-B genes among K. pneumoniae isolates with rates 20(66.66%), 11(36.66%), 22(73.33%), 3(10%) and 17(56.66%) respectively.

scholarly journals Microbiological Assessment of Fresh Expressed Breast Milk on Room Temperature at Dr. Soetomo Hospital Neonatal Unit

2020 ◽  
Vol 56 (1) ◽  
pp. 24
Author(s):  
Kartika Hardiani ◽  
Nur Aisiyah Widjaja ◽  
Meta Herdiana Hanindita ◽  
Roedi Irawan ◽  
Eddy Bagus Wasito
Keyword(s):  
Breast Milk ◽  
Klebsiella Pneumoniae ◽  
Room Temperature ◽  
Culture Media ◽  
Bacterial Species ◽  
Coagulase Negative Staphylococcus ◽  
Streptococcus Faecalis ◽  
Bacterial Colony ◽  
Significant Difference ◽  
Expressed Breast Milk

Expressed breast milk (EBM) has been advocated as an effective way for encouraging and maintaining lactation when the mother is separated from the baby for a while. Storing EBM at room temperature in several hours before consuming, frequently found in Indonesia. Based on WHO guidelines EBM can last for 6 to 8 hours in room temperature. But currently there hasn’t been study in tropical country especially Indonesia for the guidelines. Assesing microbiological quality of EBM on room temperature, including bacterial growth and major bacterial found on EBM for health care and society recommendations. An observational study of 30 expressed breast milk samples provided by 30 healthy women with term baby below 6 month old. EBM were collected by electric breast pump swing model. The samples were kept sterile and laid at plates for 2 hours, 4 hours and 6 hours in room temperature (26o- 32oC) and used drop plate technique on several culture media. Data was analyzed by Chi-square and paired sample T-test. Thirty of unheated fresh EBM from 30 lactating mothers were stored at room temperature, examined for the degree of bacterial contamination at 0 hour, 2 hours, 4 hours, and 6 hours. All the EBM samples were contaminated at 2 hour. There were strong correlation between 2 hours storing with 4 hours storing (p=0.004) and total colony (p=0.000). There were also significant difference between bacterial colony and the duration itself (p=0.026). Bacterial species identified was Coagulase-negative Staphylococcus (CNS), Escherichia coli, Klebsiella pneumoniae and Streptococcus faecalis, range of growth 109 cfu/ml - 63 x 109 cfu/mm3 after 6 hour of storage. Evaluation result that the EBM exposed at room temperature (30-36 0C) for more than two hour reduce the quality and do not recommended to be given to the infants because bacterial counts > 104 cfu/ml and the present of pathogens E. coli, Streptococcus faecalis and Klebsiella pneumoniae.

scholarly journals Antimicrobial Activity of Phoebe lanceolata and Stephania glabra; Preliminary Screening Studies

2009 ◽  
Vol 1 (3) ◽  
pp. 662-666 ◽  
Author(s):  
D. K. Semwal ◽  
U. Rawat ◽  
A. Bamola ◽  
R. Semwal
Keyword(s):  
Klebsiella Pneumoniae ◽  
Culture Media ◽  
Bacterial Species ◽  
Stem Bark ◽  
Fungal Species ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Preliminary Screening ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Ethanolic extracts of Phoebe lanceolata stem bark and Stephania glabra tubers were evaluated for their antibacterial and antifungal activities against five bacterial species, Staphylococcus aureus (along with ten hospital strains), Staphylococcus mutans, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae and six fungal species Aspergillus niger, Aspergillus fumigatus, Penicillum citranum, Microsporum gypseum, Microsporum canis, Trichophyton rubrum, obtained from different culture media. The plants/parts extracts were found active against most of the tested microorganism with MIC range of 50-100µg/ml. The MIC was taken at the lower concentration where inhibition ceased. Novobiocin (15 µg/ml) and erythromycin (15 µg/ml) were used as positive controls for bacterial and fungus species respectively.  Keywords:Antimicrobial activity; Streptococcus mutans; Klebsiella pneumoniae; Microsporum gypseum; Nutrient and Sabouraud’s dextrose agar medium. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i3.2423               J. Sci. Res. 1 (3), 662-666 (2009) 

scholarly journals Assessing the ability of Tigecycline and Meropenem to clear intra-macrophage Klebsiella pneumoniae

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Kinza Asim ◽  
Joseph J. Wanford ◽  
Marco Oggioni
Keyword(s):  
Klebsiella Pneumoniae ◽  
Culture Media ◽  
Antimicrobial Treatment ◽  
Macrophage Infection ◽  
Cell Culture Media ◽  
Treatment Regimens ◽  
Life Threatening ◽  
Monocyte Cell ◽  
Fetal Bovine ◽  
Extracellular Activity

The emergence of hypervirulent Klebsiella pneumoniae of serotype K1 and K2 are a major cause of life-threatening, community-acquired infections. Recent studies demonstrated that Kp can persist for long periods of time within the spleen and liver and survive within macrophages. We aimed to explore whether two clinically relevant antimicrobials differed in their capacity to clear within-macrophage Kp. The mouse monocyte cell line J774a were used to model Kp macrophage infection (cultured in RPMI, 10% Fetal-bovine-serum, 37oC, 5%CO2). Cells were harvested and seeded in a 96-well plate at 2x106 cells/mL and incubated overnight. The following day, cells were infected with hypervirulent, K1 Kp (NTUH-K2044) for one hour at an MOI of 10. A 1-hour treatment with gentamicin and polymyxin-Bwas used to kill extracellular Kp. Cells were then washed, and incubated with serial 2-fold dilutions of meropenem and tigecycline for 6 hours. In parallel, a killing assay was performed with antibiotic, and Kp in cell culture media alone to compare the intracellular and extracellular activity of each antibiotic. We show that whilst the majority of the inoculum was resistant to phagocytosis, a small fraction of Kp were able to adhere to macrophages, enter the cell, and persist for up to 6h. Furthermore, we demonstrate that lower concentrations of tigecycline were required to inhibit intracellular Kp, compared with meropenem, which required concentrations in excess of the planktonic MIC to clear the intracellular niche. These data indicate that there is reason to re-examine the antimicrobial treatment regimens for hypervirulent Kp infection with a focus on intracellularly active drugs.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI

2011 ◽  
Vol 15 (1) ◽  
pp. 282 ◽  
Author(s):  
Fatimawali Fatimawali ◽  
Fatmawaty Badaruddin ◽  
Irawan Yusuf
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pure Culture ◽  
Culture Media ◽  
Inorganic Mercury ◽  
River Estuary ◽  
Nutrient Broth ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mercury Resistant Bacteria

ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI Fatimawali1), Fatmawaty Badaruddin2), Irawan Yusuf 2) 1)Dosen Fakultas  Kedokteran Unsrat Manado dan Mahasiswa Program Doktor Fakultas Kedokteran Universitas Hasanuddin Makassar; 2)Dosen Fakultas Kedokteran Universitas Hasanuddin Makassar ABSTRAK Merkuri adalah logam berat yang bersifat sangat toksik, mempunyai afinitas terhadap gugus thiol protein.  Keberadaan merkuri  dilingkungan, karena secara alamiah maupun karena aktifitas antrofogenik. Di Kabupaten Bolaang Mongondow terdapat beberapa penambang emas rakyat yang menggunakan merkuri untuk mengekstrak emas dari batuan atau ore dan membuang limbah merkuri secara bebas ke lingkungan dan terbawa air hujan sampai kepada perairan.  Sedimen perairan yang terkontaminasi merkuri dapat ditumbuhi bakteri resisten merkuri.  Bakteri resisten merkuri anorhanik dapat digunakan untuk bioremediasi perairan, karena bakteri tersebut mampu mereduksi merkuri anorganik menjadi logam merkuri yang mudah menguap dan kurangtoksik.  Dan merupakan sumber pencemaran merkuri.  Bakteri resisten merkuri mempunyai operon mer yang biasanya terkandung dalam plasmid.  Penelitian ini bertujuan untuk mengisolasi bakteri resisten merkuri anorganik, dan menganalisis kemampuannya dalam mereduksi HgCl2 dalam media nutrient broth.  Bakteri resisten merkuri tinggi, dilakukan analisis Gen 16S rRNAnya untuk mengetahuis pesiesnya.  Hasil penelitian menunjukkan bahwa isolat A1.1.1 merupakan bakteri spesies Klebsiella pneumoniae, mampu mereduksi HgCl2 75% dalam waktu 1 jam, 92% dalam waktu 12 jam dan 99,4%  dalam waktu 24 jam Kata Kunci: bakteri, detoksifikasi,  HgCl2,  Klebsiella pneumoniae   ISOLATION  AND  IDENTIFICATION OF MERCURY-RESISTANT BACTERIUM FROM SARIO RIVER ESTUARY THAT CAN BE USED TO DETOXIFY INORGANIK MERCURY WASTES ABSTRACT Mercury (Hg) is well known for its high toxicity and strong affinity toward the thiol group of proteins. When Hg released into the environment in substantial quantities through natural events and anthropogenic activities . In District of Bolaang Mongondow, many miners who are a source of mercury pollution of aquatic environments to which they use to extract gold from the rock or ore. Waters sediment contaminated with mercury, can be overgrown with microorganism such as bacteria that are resistant to mercury.  Mercury-resistant bacteria can be used for bioremediation of waters due to the ability of these bacteria to reduce toxic inorganic mercury into mercury metal which is volatile and less toxic. Mercury-resistant bacteria has mer Operon which usually contained in the plasmid.  The aim of this study was the selection of bacterial strains resistant to inorganic mercury, as well as to show their capacity to reduce mercury  in pure culture media Nutrient Broth. Twelve isolate bacteria was selected for its capacity to reduce mercury HgCl2 in culture media nutrient broth. The one bacterial strains belong to the spesies Klebsiella pneumoniae. Inoculated in pure culture, these strain showed a mercury reduction of 75% in 1 hour, 92% in 12 hours, and  99,4% in 24 hours. Keywords : bacteria , detoxification, HgCl2 ,  Klebsiella pneumoniae

scholarly journals Profiling urinary tract infections bacteria among elderly population in a Nigerian Teaching Hospital

2018 ◽  
Vol 6 (3) ◽  
pp. 15-22
Author(s):  
S. A. Saka ◽  
B. E. Okunuga
Keyword(s):  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Teaching Hospital ◽  
University Teaching ◽  
Resistance Pattern ◽  
Biomedical Sciences ◽  
Significant Bacteriuria ◽  
Tract Infections ◽  
Urine Specimens

There have been conflicting reports about prevalence of Urinary Tract Infections (UTIs) causing bacteria in elderly in recent times. This study aims to evaluate the prevalence and resistance pattern of UTIs causing bacteria in elderly Nigerian patients. A prospective cross-sectional study was carried out among elderly patients attending the general and medical outpatients’ clinics of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria. Patients aged 60 years and above with at least two signs of UTIs were purposefully selected for the study. Clean catch mid-stream urine specimens from 100 eligible patients were examined for significant bacteriuria. Identification and antibiotics susceptibility patterns of the isolates were determined using standard techniques. Data were analysed using descriptive statistics such as frequency and percentage. Association between variables was determined using Chi-squared test. P values<0.05 were considered significant. One hundred elderly outpatients were evaluated. Majority of the study participants were males (68, 68.0%) and (64, 64.0%) were married. More than half of the participants (59, 59.0%) had no significant bacteriuria. Among the participants with significant bacteriuria (41, 41.0%), males (29/41, 70.7%, p=0.001) were more than the females (12, 29.3%). Klebsiella pneumoniae (19/41, 46.3%) was the most isolated organism in the participants’ urine specimens, (35/41, 85.4%) of the isolates were resistant to nitrofurantoin. Klebsiella pneumoniae was the most isolated UTIs-causing bacteria among the elderly evaluated. Physicians need to be aware of trends in profiles of UTIs-causing bacteria for effective diagnosis of the disease in elderly.Journal of Medical and Biomedical Sciences (2017) 6(3), 15 - 22

scholarly journals Multiple Drug Resistance Pattern in Urinary Tract Infection Patients in Saudi Arabia

2019 ◽  
Vol 6 (1) ◽  
pp. 3-7
Author(s):  
Shamweel Ahmad
Keyword(s):  
Drug Resistance ◽  
Urinary Tract Infection ◽  
Saudi Arabia ◽  
Urinary Tract ◽  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
Resistance Pattern ◽  
Antibiotic Prescribing ◽  
Drug Resistance Pattern ◽  
Urine Specimens

Background: Urinary tract infection (UTI) is the most common problem in hospitalized and outdoor patients. It is mainly found in females because of the shortness of the urethra and closeness to anus, which facilitate entrance of fecal micro–flora to urinary tract. Knowledge of local antimicrobial resistance patterns is essential for evidence- based empirical antibiotic prescribing. Objective: The main aim of this study was to analyze the drug resistance pattern of bacterial isolates from suspected urinary tract infection (UTI) patients in Al-Kharj, Saudi Arabia. Methodology: This cross- sectional study was carried out in Al-Kharj region of Saudi Arabia from 1st. September, 2016 to 28th. February, 2017. Midstream urine specimens were collected from patients at a general hospital. The specimens were cultured and the isolates were identified using standard microbiological techniques. The antibiotic susceptibilities of the isolates were also determined. Results: The number of patients with urinary tract infection who yielded positive cultures from their mid stream urine specimens was 249(12.0%) out of 2064 specimens. The commonest isolates were Escherichia coli (53.4%) and Klebsiella pneumoniae (28.5%). Other bacterial pathogens were Proteus mirabilis (5.2%), Pseudomonas aeruginosa (4.4%), Streptococcus agalactiae (6.0%) and Enterococcus faecalis. (2.5%). Conclusions: E. coli is the most common causative agent of urinary tract infection followed by Klebsiella pneumoniae. Ampicillin, Augmentin, Cotrimoxazole, Norfloxacin and Nalidixic acid have the highest resistance rates against both these pathogens. No isolate is found to be resistant to imipenem. Bangladesh Journal of Infectious Diseases 2019;6(1):3-7

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