scholarly journals Quality Control Of Crude Drugs And Capsules Of Turmeric (Curcuma longa L.)

2018 ◽  
Vol 15 (2) ◽  
pp. 257
Author(s):  
Dwi Hartanti ◽  
Jirapat Theeravit
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Oil Content ◽  
Curcuma Longa ◽  
Volatile Oil ◽  
Water Soluble ◽  
Foreign Matter ◽  
Single Use ◽  
Crude Drugs ◽  
Layer Chromatography

Turmeric (Curcuma longa L.) is a medicinal plant commonly used in Thai Traditional Medicine (TTM), both in single use and formula. It has been known for its antioxidant activity and applied for management of many diseases. In this article, we reported some aspects of quality control of crude drugs and capsules of turmeric according to the standards set in Thai Herbal Pharmacopeia (THP). The results of our evaluations demonstrated that turmeric crude drugs met the criteria set in THP for microscopical identification, foreign matter (1.31%), loss on drying (6.89±0.174%), ethanol-soluble extractive (13.56%), water-soluble extractive (15.17%), and the profile of Thin Layer Chromatography (TLC) chromatogram. However, its volatile oil content (5.95%) was below the minimum value set in THP. The turmeric capsules met the criteria set in THP for loss on drying (8.64±0.093%), ethanol-soluble extractive (18.07%), water-soluble extractive (14.95%), and profile of TLC chromatogram.

scholarly journals Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

2019 ◽  
Vol 102 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Wan-Xin Zhang ◽  
In-Cheng Chao ◽  
De-jun Hu ◽  
Farid Shakerian ◽  
Liya Ge ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Aerial Parts ◽  
Alpinia Officinarum ◽  
Layer Chromatography ◽  
Different Parts ◽  
Tof Ms

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

scholarly journals ANÁLISE FARMACOPÉICA DE AMOSTRAS DE CAMOMILA Matricaria recutita L., ASTERACEAE

2003 ◽  
Vol 4 (2) ◽  
Author(s):  
M. Do R. DUARTE ◽  
M. P. De LIMA
Keyword(s):  
Oil Content ◽  
Volatile Oil ◽  
Morphological Identification ◽  
Foreign Matter ◽  
Drug Quality ◽  
Matricaria Recutita ◽  
De Se ◽  
Annual Herb

Matricaria recutita L. é uma herbácea anual, popularmente denominada de camomila e empregada como antiinflamatório e antiespasmódico. Este trabalho objetivou proceder à análise farmacognóstica de amostras de camomila comercializadas em Curitiba, a fim de se avaliar a qualidade desse fármaco vegetal e do mercado fitoterápico. As amostras foram ensaiadas de acordo com a monografia farmacopéica oficial, compreendendo a caracterização organoléptica, identificação morfológica, pesquisa e doseamento de óleo essencial e determinação de matéria estranha. Todas as oito amostras apresentaram características organolépticas, macro e microscópicas correspondentes ao capítulo floral, no entanto cinco delas excederam o teor máximo de matéria orgânica estranha e apenas uma apresentou o teor mínimo de óleo essencial estabelecido, sendo esta a única amostra considerada satisfatória. PHARMACOPOEICAL ANALYSIS OF CHAMOMILE SAMPLES - Matricaria recutita L., ASTERACEAE Abstract Matricaria recutita L. is an annual herb, commonly called as chamomile and employed as antiinflammatory and antispasmodic. This work carried out a pharmacognostical analysis of chamomile samples commercialized in Curitiba, in order to evaluate the drug quality and the phytotherapy market. The samples were essayed according to the official pharmacopoeia monograph, consisting of the organoleptical characterization, morphological identification, volatile oil content and foreign matter determination. All eight samples have presented the correspondent organoleptical, macro and microscopical characters, however five have exceeded the maximum foreign matter value, and only one has got the required volatile oil content and was approved.

scholarly journals Identification of the Appropriate Curing Method for Sri Lankan Turmeric (Curcuma longa L.) and Compare its Phytochemical Properties with an Indian Market Sample

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Chathuri Senanayake ◽  
Anuradha Munasinghe ◽  
Thushari Liyanage
Keyword(s):  
Oil Content ◽  
Curcuma Longa ◽  
Volatile Oil ◽  
High Yield ◽  
Sri Lankan ◽  
Drying Time ◽  
Significant Step ◽  
Curing Method ◽  
Processing Steps

Turmeric (Curcuma longa L.) is an underground rhizome that is an essential ingredient in day-to-day life. India is the major turmeric production and exportation country in the world. However, since December 2019, Sri Lanka has banned the importation of turmeric from other countries like India. Curing is a significant step during the processing of turmeric, which can be described as cooking fresh turmeric rhizomes before going to the drying step. Curing helps to avoid the raw odor, to reduce the drying time, to distribute the pigment product uniformly, and facilitate uniform drying. This study was conducted to identify the best protocol for turmeric processing and compare the quality of local turmeric samples prepared by using different processing steps such as, uncured and dried as slices, water boiled and dried as slices, steamed and dried as slices with imported turmeric from India. All the samples were separately analyzed for phytochemical properties (moisture content, volatile oil content, curcumin content, oleoresin content, etc.). Curcumin is an important attribute when considering the quality of turmeric. Curcumin content is varied with the curing step. The lowest curcumin content was shown by the uncured and sliced sample (2.86 ± 0.13 %). Steamed and dried as slices sample showed the highest curcumin content (4.51 ± 0.02 %). Curing and Slicing also decreased the drying time. However, water boiled and sliced sample (T-2) showed a high yield (wet to dry ratio 5.41:1), volatile oil content 4.40 ± 0.00 %, and oleoresin content 14.81 ± 2.36 %. According to the results, among the locally produced turmeric, steamed and dried as slices sample (T-3) showed good quality especially for curcumin content.

scholarly journals Validated high-performance thin-layer chromatographic analysis of curcumin in the methanolic fraction of Curcuma longa L. rhizomes

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Poonam Kushwaha ◽  
Babita Shukla ◽  
Jyotsana Dwivedi ◽  
Sumedha Saxena
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Curcuma Longa ◽  
Dry Weight ◽  
Good Separation ◽  
Chromatography Method ◽  
Crude Drugs ◽  
Thin Layer Chromatographic Analysis ◽  
Layer Chromatography ◽  
Absorption Mode

Abstract Background In the present study, an HPTLC (high-performance thin-layer chromatography) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L. For achieving good separation of curcumin, the mobile phase of chloroform:methanol (97:3) was used. The densitometric analysis of curcumin was performed at 420 nm in reflection/absorption mode. Results Linearity of the method was obtained in the range of 100‒600 ng per spot. During analysis, the methanolic fraction of the C. longa showed the presence of a quantifiable amount of curcumin. The content of curcumin was found to be 1.5% (per dry weight). Conclusions The method is specific, simple, precise, and accurate. The obtained data can have used for the routine analysis of the reported biomarkers in crude drugs and extracts. The quantification and the method validation of curcumin have not yet been reported in C. longa which can be utilized for the proper standardization of the plant.

scholarly journals Standardization of the Ayurvedic Formulation Haridra Khanda Using High-Performance Thin-Layer Chromatography-Densitometry

2008 ◽  
Vol 91 (5) ◽  
pp. 1162-1168 ◽  
Author(s):  
Kedar Kumar Rout ◽  
Sagarika Parida ◽  
Sagar Kumar Mishra
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Curcuma Longa ◽  
Water Soluble ◽  
Trace Metal Analysis ◽  
Cell Structures ◽  
Microscopic Evaluation ◽  
Multiple Wavelengths ◽  
Layer Chromatography ◽  
Instrumental Precision

Abstract The present study aimed to standardize the Ayurvedic preparation Haridra Khanda containing Curcuma longa as a major ingredient. Various physicochemical parameters such as alcohol-soluble extractive, water-soluble extractive, total ash, and acid-insoluble ash were determined according to the Ayurvedic Pharmacopoeia of India. Microscopic evaluation of the formulation revealed the presence of various diagnostic cell structures of C. longa. Trace metal analysis indicated the absence of toxic metals such as As, Cd, Hg, and Pb. High-performance thin-layer chromatographic (HPTLC) fingerprint patterns at multiple wavelengths (254, 366, and 430 nm) identified the number of components present at each wavelength. The bioactive markers curcumin (C1), demethoxycurcumin (C2), and bisdemethoxycurcumin (C3) were quantified by using a simple, rapid, and efficient HPTLC method using plates precoated with silica gel 60F254 stationary phase. The instrumental precision [coefficient of variation (CV)] was 0.51, 0.64, and 0.79% and the repeatability of the method (CV) was 0.89, 1.11, and 0.95%, respectively, for C1 to C3. Limits of detection and quantitation for compounds C1 to C3 were 20, 20, and 15 ng and 50, 40, and 50 ng, respectively. Response was a linear function in the ranges of 50350, 40240, and 50300 ng with correlation coefficient (r) 0.9998, 0.9995, and 0.9992, respectively, for C1 to C3. The mean recovery values of 99.63 (C1), 98.65 (C2), and 98.97% (C3) indicated the excellent accuracy of the method. It is shown that HPTLC can be applied successfully for the marker evaluation of the formulation containing C. longa.

scholarly journals Pharmacognostical characterization and in vitro antioxidant activity of Acacia pennata (L.) Willd. leaves: A Southeast Asian medicinal plant

2020 ◽  
Vol 20 (1) ◽  
pp. 16-28
Author(s):  
James H. Zothantluanga ◽  
Ngurzampuii Sailo ◽  
Arpita Paul ◽  
Anshul Shakya
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Methanolic Extract ◽  
Chromatographic Fingerprint ◽  
Diagnostic Features ◽  
Accurate Identification ◽  
Qualitative And Quantitative ◽  
Layer Chromatography ◽  
In Vitro Antioxidant Activity

Pre-defined analytical features of a plant are used as a reference to aid in accurate identification, proper standardization, and quality control. However, no systematic work on the leaves of A. pennata is available for reference to check its authenticity, purity, and quality. Therefore, the study was aimed to develop pharmacognostic parameters using standard guidelines. The work provides macroscopic, qualitative and quantitative microscopic, physicochemical parameters, chromatographic fingerprint profile, and in-vitro antioxidant activity of A. pennata. Preliminary phytochemical screening and thin-layer chromatography hints the presence of steroids and glycosides along with polyphenolic compounds viz. phenols and flavonoids. Quantification of polyphenolic phytoconstituents and assessment of in-vitro antioxidant activity of the methanolic extract was done. The current communication offers referential knowledge on the analytical and diagnostic features for accurate taxonomical identification, proper characterization and will also help in the establishment of a pharmacognostic monograph of A. pennata for effective quality control.

scholarly journals Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 357
Author(s):  
Md Khairul Islam ◽  
Tomislav Sostaric ◽  
Lee Yong Lim ◽  
Katherine Hammer ◽  
Cornelia Locher
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
High Performance ◽  
Thermal Exposure ◽  
Bioactive Constituents ◽  
Potential Value ◽  
Novel Approach ◽  
Depth Study ◽  
Layer Chromatography ◽  
Over Time

The use of High-Performance Thin-Layer Chromatography (HPTLC) coupled with the use of DPPH* (2,2-diphenyl-1-picrylhydrazyl) as a derivatisation reagent is a novel approach to the analysis of antioxidant activity of honeys. The method facilitates the visualisation of individual constituents that contribute to the overall antioxidant activity of the honey, even if they are not yet chemically identified, and allows for the quantification of their antioxidant activity as gallic acid equivalents. The method supports a more in-depth study of the antioxidant activity of honey as it allows for a comparative analysis of the antioxidant fingerprints of honeys of different floral origin and is able to capture differences in their individual bioactive constituents. Further, it supports the tracking of changes in antioxidant activity of individual honey constituents over time upon exposure to different temperature conditions, which demonstrates the potential value of the method for in-process quality control.

scholarly journals PHARMACOGNOSTIC AND HPTLC BASED COMPARATIVE STUDY ON LEAVES OF MERREMIA EMARGINATA BURM. F. AND CENTELLA ASIATICA (L.) URBAN

2019 ◽  
pp. 29-37
Author(s):  
MANJU K. C. ◽  
ANITHA JOHN ◽  
SAKKEENA A. ◽  
GAYATHRI DEVI V. ◽  
NEETHU KANNAN B. ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Physicochemical Parameters ◽  
Centella Asiatica ◽  
Volatile Oil ◽  
Ash Content ◽  
Water Soluble ◽  
Calcium Oxalate Crystal ◽  
Plant Materials ◽  
Standard Methods ◽  
Crude Drugs

Objective: In this study, an attempt was made to generate information based on botanical, physicochemical and HPTLC data needed for proper identification and authentication of M. emarginata and C. asiatica belonging to two different families. Methods: Botanical study comprises of macroscopy, microscopy and powder microscopy of leaves of both crude drugs. The physicochemical parameters such as water-soluble extractive, alcohol soluble extractive and loss on drying at 105℃, total ash, acid insoluble ash, and volatile oil were determined according to standard methods. HPTLC studies of chloroform extracts of leaves of both drugs were conducted at 254 nm, 366 nm and 575 nm after derivatisation using vanillin-sulphuric acid and the results were documented. Results: The present study reveals that microscopy and most of the physicochemical parameters of both the plant materials are different. Anatomy of the leaves showed two main characteristic differences. First plenty of trichome with trichome base and calcium oxalate crystal is common in M. emarginata, which is not observed in C. asiatica. Both plants have different venation patterns and leaf constants. The total ash content and the solubility in alcohol and water for leaves of C. asiatica are higher than that of M. emarginata. The HPTLC fingerprinting pattern obtained for both drugs are different. Conclusion: All the results obtained from this study help in determining differences and similarities of leaves of M. emarginata and C. asiatica and thereby preventing adulteration and substitution and emphasizing the importance of standardization.

scholarly journals Comparative pharmacognostical and pharmaceutical evaluation of Vyaghri haritaki avaleha - an ayurvedic formulation

2015 ◽  
Vol 3 (02) ◽  
pp. 07-12
Author(s):  
Suhas A Chaudhary ◽  
K S Patel ◽  
V S Kori ◽  
S Rajagopala ◽  
C R Harisha ◽  
...  
Keyword(s):  
High Performance ◽  
Ph Value ◽  
Medical Science ◽  
Research Work ◽  
Water Soluble ◽  
Cough Reflex ◽  
Crude Drugs ◽  
Water Soluble Extract ◽  
Work Done ◽  
Layer Chromatography

Kasa is well-defined clinical condition in Brihadtrayi, clearly correlate with cough and its pathophysiology exactly correlates with the mechanism of cough reflex in contemporary medical science. Everywhere, Paediatric Outdoor Patients Department (OPD) has more than half of the total patients having respiratory tract complaints. So, to treat the disease Kasa, Vyaghri Haritaki Avaleha (VHA) was taken from Bhaishajya Ratnavali. The present work was carried out to standardize the raw drugs and finished product-VHA with comparing API standards and previous research work done in same institute to conform its identity, quality and purity of the final product. The pharmacognostical work reveals that presence of Epidermis, Cork, Pericycle, Phloem etc. from Kantakari; Lignified fibre, Stone cells etc. of Haritaki; Aluerone grains, Prismatic crystals etc. of Shunthi observed microscopically. Organoleptic features of VHA made out of the crude drugs were within the standard range. The pH value of VHA was 4.5, Water soluble extract was 71.9 %w/w, Loss on drying was 30.41 %w/w, Reducing sugar was 27.92 %w/w and High Performance Thin Layer Chromatography (HPTLC) at 254nm and 366nm resulted into 2 and 5 spots respectively

scholarly journals Chemical analysis of turmeric from Minas Gerais, Brazil and comparison of methods for flavour free oleoresin

1998 ◽  
Vol 41 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Cyleni R. A. Souza ◽  
Maria Beatriz Abreu Glória
Keyword(s):  
Chemical Analysis ◽  
Oil Content ◽  
Curcuma Longa ◽  
Volatile Oil ◽  
Minas Gerais ◽  
Ether Extract ◽  
Protein Fiber ◽  
Food Applications ◽  
The Mean ◽  
Extract Protein

Chemical analysis of turmeric (Curcuma longa L) cultivated in eight different cities in the state of Minas Gerais, Brazil was carried out. The levels of curcuminoid pigments varied from 1.4 to 6.14 g/100 g and of volatile oil from 0.97 to 7.55 mL/100 g (dry basis). Samples from Patrocínio, Arinos and Brasilândia contained higher pigment levels compared to the others. The sample from Patrocínio contained the highest volatile oil content. The mean levels of ethyl ether extract, protein, fiber, ash and starch were 8.51, 7.01, 7.22, 7.81 and 39.87 g/100 g dry basis, respectively. Laboratory extraction of flavour free oleoresin was performed in triplicate. A higher yield of pigment in the oleoresin was obtained when the volatile oil was extracted with water vapor and the oleoresin with ethanol. The oleoresin obtained was free of flavour and could be used in a wider range of food applications.

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