Uji Biokompabilitas pada Implan Orthopedi Antara Implan Impor, Implan Lokal dari Material Impor, dan Prototipe Stainless Steel 316L dari Material Lokal

ABSTRACT Demands of orthopedic implants are increasing in Indonesia, but its production are limited and still imported. Stainless steel, especially 316L is one of metal that is used commonly. This research evaluates biocompatibility of stainless steel 316L implant prototype, local implant manufactured using imported stainless steel, and imported stainless steel implant. Cytotoxicity test used mesenchymal human cell with MTT assay. Irritation test, acute toxicity/pyrogenicity test, and implantation test used 24 Wistar rats respectively which were assigned to 4 groups. We evaluate Draize score, weight and rectal temperature changes, and fibrous capsule ring thickness. Cytotoxicity test shows viable cell percentage in stainless steel 316L implant prototype, local implant, and imported implant are 83.7%, 87.5%, and 83.7% respectively. Comparison test using One Way ANOVA shows no significant difference between 3 groups (p>0.05). Iritation test results in 3 groups are grade 0 (no edema and no erythema). Acute toxicity/pyrogenicity test shows no statistically significant difference (p>0.05) among all groups. There is also no significant difference (p>0.05) of fibrous capsule ring thickness on 1 and 4 weeks after implantation. Based on ISO for medical test guide, stainless steel 316L implant prototype is safe and has similar biocompatibility test result compared to local and imported implant.Keywords : stainless steel 316L, biocompatibility, orthopaedic implantCorrespondence to : [email protected] ABSTRAKKebutuhan implan orthopedi terus meningkat di Indonesia, namun produksinya terbatas dan sebagian besar masih impor dari luar negeri. Stainless steel, utama 316L adalah salah satu metal yang sering digunakan. Penelitian ini mengevaluasi biokompatibilitas dari prototipe implan stainless steel 316L, implan lokal yang diproduksi menggunakan stainless steel impor, dan implan stainless steel impor. Uji sitotoksisitas menggunakan sel mesenkim human dengan MTT assay. Uji iritasi, uji pirogenositas, dan uji implantasi menggunakan 24 tikus Wistar yang terbagi dalam 4 grup. Kami mengevaluasi skor Draize, perubahan berat dan temperatur rektal, dan ketebalan fibrous capsule ring. Uji sitotoksisitas menunjukkan persentase viabilitas sel pada prototipe stainless steel 316L, implan lokal, dan implan impor berturut-turut adalah 83,7%; 87,5%; dan 83,7%. Uji komparasi dengan One Way Anova tidak menunjukkan perbedaan signifikan diantara 3 grup (p>0,05). Hasil uji iritasi 3 grup menunjukkan grade 0 (tidak ada edema dan eritema). Uji pirogenositas tidak menunjukkan perbedaan signifikan pada semua grup (p>0,05). Tidak ada perbedaan signifikan (p>0,05) pula pada ketebalan fibrous capsule ring di minggu ke 1 dan 4 setelah implantasi. Berdasarkan ISO untuk pedoman uji di bidang medis, prototipe stainless steel 316L aman dan memiliki kesamaan hasil uji biokompatibilitas bila dibandingkan dengan implan lokal dan impor.Kata kunci : stainless steel 316L, uji biokompatibilitas, implan orthopaediKorespondensi : [email protected]

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CYTOTOXICITY OF IONS RELEASED FROM 17-4 PRECIPITATION HARDENING STAINLESS STEEL ORTHODONTIC BRACKETS IN ARTIFICIAL SALIVA

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s2.17 ◽

2018 ◽

Vol 9 ◽

pp. 71

Author(s):

Tjokro Prasetyadi ◽

Bambang Irawan ◽

Miesje Karmiati Purwanegara ◽

Bambang Suharno ◽

Sugeng Supriadi

Keyword(s):

Stainless Steel ◽

Cell Viability ◽

Precipitation Hardening ◽

Artificial Saliva ◽

Nickel Content ◽

Orthodontic Brackets ◽

Assay Method ◽

Cytotoxicity Test ◽

Significant Difference

Objective: 17-4 precipitation hardening (PH) stainless steel has a low nickel content, which can reduce the risk of allergic reactions. It also has good mechanical properties against the stress caused by the archwire slot brackets in orthodontic treatments. The main focus of this study to evaluate the metal ions released into artificial saliva from different orthodontic brackets with the same 17-4 PH stainless steel and to examine the in vitro cytotoxicity of the metal.Methods: Material properties were analyzed by energy dispersive spectroscopy. The 3-(4,5-dimethylthiazol-2-y1)2,5-diphenyltetrazolium bromide (MTT) assay method was used to examine the cytotoxicity of Gemini and Synergy brackets.Results: The cytotoxicity test on all the orthodontic brackets showed a mean cell viability value above 80% in each immersion group, which means that this material is not cytotoxic to the human immortalized keratinocyte cell line.Conclusions: The results showed cell viability in the extracts of both groups of brackets, and there was no statistically significant difference between the groups (p>0.05).

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Flexural Strength and Cytotoxicity of ZCC, a Resin Modified Zinc-Calcium Liner Prototype

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.751.649 ◽

2017 ◽

Vol 751 ◽

pp. 649-656

Author(s):

Pasutha Thunyakitpisal ◽

Nonglax Thunyakitpisal ◽

Sirithan Jiemsirilers ◽

Dujreutai Pongkao Kashima

Keyword(s):

Flexural Strength ◽

Mtt Assay ◽

Fetal Bovine Serum ◽

Growth Media ◽

Human Dental Pulp Cells ◽

Significant Difference ◽

Human Dental Pulp ◽

Pulp Cells ◽

Fetal Bovine ◽

One Way Anova

Objective: To investigate the flexural strength and cytotoxicity of ZCC, a resin modified zinc oxide-calcium carbonate liner prototype, compared with commercial dental liners. Materials and Methods: ZCC, Dycal, Ultra-Blend® plus (UB), and TheraCal LC® (TC) were evaluated for flexural strength. Six samples of each material were incubated in growth medium (10% fetal bovine serum supplemented DMEM) for 24 h. Primary human dental pulp cells were cultured in the conditioned medium from each sample, with growth media used as a control. Cytotoxicity was determined using an MTT assay. Data were analyzed by one-way ANOVA. The value of p<0.05 was considered significant. Results: UB had the highest flexural strength with Dycal presenting the lowest values (p<0.05). ZCC demonstrated significantly higher flexural strength values compared with those of TC and Dycal (p<0.05). The MTT assay indicated that Dycal conditioned media significantly reduced cell viability at 24 and 48 hours (p<0.05). There was no significant difference in viability between the control, ZCC, UB, and TC groups at 24 and 48 hours (p>0.05). Conclusion: ZCC met the requirements for flexural strength per ISO 9917-2:2010(E). There was no significant difference in viability between the control and ZCC group at 24 and 48 hours.

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Comparison of frictional resistance between four types of brackets in combination with stainless steel and beta-titanium archwires

APOS Trends in Orthodontics ◽

10.25259/apos_43_2019 ◽

2020 ◽

Vol 10 ◽

pp. 231-237

Author(s):

Shweta Nagesh ◽

Nagarajshetty Praveen ◽

Reddy Sumitra

Keyword(s):

Stainless Steel ◽

Frictional Resistance ◽

Static Friction ◽

Human Saliva ◽

Beta Titanium ◽

Significant Difference ◽

Ceramic Brackets ◽

Post Hoc ◽

Sliding Mechanics ◽

One Way Anova

Objectives: The objective of the study was to evaluate and compare the frictional resistance generated by four different types of brackets in combination with stainless steel (SS) and titanium molybdenum alloy (TMA) archwires. Materials and Methods: Maxillary premolar brackets were used in this study. These brackets were divided into eight groups comprising seven samples each. Of the eight groups, four groups were combined with SS and four groups were combined with TMA archwires. The testing was done in the presence of human saliva. The static frictional resistance was calculated for each group. One-way ANOVA and post hoc Tukey tests were done to compare the friction generated by each group. Results: There was a statistically significant difference between the friction generated by the monocrystalline brackets and the other bracket groups (P < 0.001). There was no statistically significant difference in static friction generated between self-ligating and conventionally ligated brackets. There was a statistically significant difference between the frictional resistance produced by SS and TMA wires (P = 0.02) with regard to monocrystalline ceramic brackets only. Conclusion: Monocrystalline ceramic brackets (Radiance) were found to generate the highest frictional resistance during sliding mechanics when compared to other brackets in combination with both SS and TMA wires. Self-ligating brackets did not show a statistically significant reduction in friction when compared to conventional ligation. There was a statistically significant difference between SS and TMA wires when used with monocrystalline brackets.

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Artemisinin (ART) Drug Delivery Using Mixed Non-ionic Surfactants and Evaluation of Their Efficiency in Different Cancer Cell Lines

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v4i4.8861 ◽

2017 ◽

Vol 4 (4) ◽

Author(s):

Elnaz Asgharkhani ◽

Aazam Najmafshar ◽

Mohsen Chiani

Keyword(s):

Zeta Potential ◽

Cell Line ◽

Mtt Assay ◽

Therapeutic Index ◽

Stability Study ◽

Polydispersity Index ◽

Ionic Surfactants ◽

Cytotoxicity Test ◽

Molar Ratios ◽

Chemotherapy Agent

This study aims to investigate the effects of different non-ionic surfactants on physicochemical properties of ART niosomes. ART is a natural compound that is used as an antimalarial and chemotherapy agent in medicine. ART has low bioavailability, stability and solubility. In order to solve these problems and enhancing the efficiency of the drug, nanotechnology was used. In the present study, several niosomal formulations of ART prepared using different molar ratios of Span 60 : Tween 60 : PEG-600: ART in PBS. These three formulations were FI (1:1:0.5:0.5), FII (2:1:0.5:0.5) and FIII (1:2:0.5:0.5), respectively. The encapsulation efficiency was measured by HPLC and the drug release was evaluated by dialysis method. The cytotoxicity test was determined by MTT assay. The size, zeta potential and polydispersity index of the vesicles was measured by Zeta Sizer. Stability study was performed within two months. The MTT assay results showed that cytotoxicity effect of these formulations on MCF-7 cell line is better than C6 cell line and the FIII had the best results for both of them. The entrapment efficiencies of the formulations I, II and III were obtained 82.2±1.88%, 75.5±0.92% and 95.5±1.23%, respectively. The results of size, zeta potential and polydispersity index indicated that the size of the vesicles is below 200 nm, their surface charge is about -35 mV and they were monodisperse. Stability and release study indicated that the formulation III has the best stability and release pattern. Therefore, the use of PEGylated niosomal ART can effectively improve its therapeutic index, stability and solubility.

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Transplantation of coral fragment, Acropora formosa (Scleractinia)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7295 ◽

2014 ◽

pp. 1

Author(s):

Hanny Tioho ◽

Maykel A.J Karauwan

Keyword(s):

Fragment Size ◽

Observation Time ◽

Minimum Size ◽

Average Growth Rate ◽

Average Growth ◽

Significant Difference ◽

Acropora Formosa ◽

Survival And Growth ◽

One Way Anova ◽

Better Than

The minimum size of coral transplants, Acropora formosa, was assessed to support their survival and growth. For this, 150 coral fragments of different sizes (5, 10, 15 cm) were transplanted close to the donor colony. Their survivorship and growth were observed for 12 months. At the end of the observation time, 90% of 15 cm-transplanted coral fragments survived, while the others (10cm and 5 cm) did 86% and 82% respectively. The average growth rate of 5 cm-coral fragments was 0.860 cm/month, while 10 and 15 cm-fragments were 0.984 cm/month and 1.108 cm/month respectively. One-way ANOVA showed that there was significant difference (p<0.05) among the three (5, 10, 15 cm) transplant initial sizes in which the longest fragment size tended to survive longer than the smaller one. However, the smaller transplants grew better than the bigger one, 10.318 cm/year (206%) for 5 cm-transplant, 11.803 cm/year (118%) for 10 cm-transplant, and 13.299 cm/year (89%) for 15 cm-transplant, respectively. Ukuran minimal fragmen karang Acropora formosa yang ditransplantasi diduga untuk mendukung ketahanan hidup dan pertumbuhannya. Untuk itu, 150 fragmen karang ditransplantasi ke lokasi yang berdekatan dengan koloni induknya. Ketahanan hidup dan pertumbuhan semua fragmen karang yang ditransplantasi diamati selama 12 bulan. Pada akhir pengamatan, 90% dari fragmen karang berukuran 15 cm yang ditransplantasi dapat bertahan hidup, sedangkan yang lainnya (ukuran 10 cm dan 5 cm) masing-masing sebesar 86% dan 82%. Rata-rata laju pertumbuhan fragmen karang dengan ukuran awal 5 cm adalah 0,860 cm/bulan, sedangkan ukuran fragmen 10 dan 15 cm masing-masing adalah 0,984 cm/bulan and 1,108 cm/bulan. ANOVA satu arah menunjukkan adanya perbedaan yang nyata (p<0.05) antara ketiga ukuran fragmen yang berbeda, di mana ukuran fragmen karang yang lebih panjang cenderung mempunyai ketahanan hidup yang lebih baik. Namun demikian, ukuran transplant yang lebih kecil memiliki pertumbuhan lebih baik dibandingkan dengan ukuran yang lebih besar, yakni10,318 cm/tahun (206%) untuk transplant berukuran 5 cm, 11,803 cm/tahun (118%) untuk 10 cm, dan 13,299 cm/tahun (89%) untuk ukuran 15 cm.

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Effect of Different Coating Techniques with Aluminum on the Corrosion Behavior of Stainless Steel 316L in Seawater

Journal of Al-Nahrain University-Science ◽

10.22401/jnus.15.3.16 ◽

2012 ◽

Vol 15 (3) ◽

pp. 112-122

Author(s):

Ali H. Ataiwi ◽

◽

Abdul Khaliq F. Hamood ◽

Rana A. Majed ◽

◽

...

Keyword(s):

Stainless Steel ◽

Corrosion Behavior ◽

Stainless Steel 316L

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Texture dependent strain hardening in additively manufactured stainless steel 316L

Materials Science and Engineering A ◽

10.1016/j.msea.2021.141483 ◽

2021 ◽

pp. 141483

Author(s):

Deepak Kumar ◽

Gyan Shankar ◽

K.G. Prashanth ◽

Satyam Suwas

Keyword(s):

Stainless Steel ◽

Strain Hardening ◽

Stainless Steel 316L ◽

Dependent Strain

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Experimental and Numerical Evaluation of Mechanical Properties of 3D-Printed Stainless Steel 316L Lattice Structures

Journal of Materials Engineering and Performance ◽

10.1007/s11665-021-05737-w ◽

2021 ◽

Author(s):

M. Carraturo ◽

G. Alaimo ◽

S. Marconi ◽

E. Negrello ◽

E. Sgambitterra ◽

...

Keyword(s):

Mechanical Properties ◽

Stainless Steel ◽

Mechanical Behavior ◽

Numerical Simulations ◽

Uniaxial Tensile ◽

Uniaxial Tensile Test ◽

Lattice Structures ◽

Stainless Steel 316L ◽

Research Attention ◽

Octet Truss

AbstractAdditive manufacturing (AM), and in particular selective laser melting (SLM) technology, allows to produce structural components made of lattice structures. These kinds of structures have received a lot of research attention over recent years due to their capacity to generate easy-to-manufacture and lightweight components with enhanced mechanical properties. Despite a large amount of work available in the literature, the prediction of the mechanical behavior of lattice structures is still an open issue for researchers. Numerical simulations can help to better understand the mechanical behavior of such a kind of structure without undergoing long and expensive experimental campaigns. In this work, we compare numerical and experimental results of a uniaxial tensile test for stainless steel 316L octet-truss lattice specimen. Numerical simulations are based on both the nominal as-designed geometry and the as-build geometry obtained through the analysis of µ-CT images. We find that the use of the as-build geometry is fundamental for an accurate prediction of the mechanical behavior of lattice structures.

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