scholarly journals Using Principal Component Analysis and RNA-Seq to Identify Candidate Genes That Control Salt Tolerance in Garlic (Allium sativum L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 691
Author(s):  
Xiangjun Zhou ◽  
Yanxia Dou ◽  
Xiaoxia Huang ◽  
Gang Li ◽  
Hongrui Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Principal Component ◽  
Dry Weight ◽  
Component Analysis ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Leaves And Roots

To examine physiological responses of garlic to salinity, 17-day-old seedlings of eight soft-neck accessions were treated with 200 mM NaCl for seven days in a hydroponic system. Several morphological and physiological traits were measured at the end of the treatment, including shoot height, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, photosynthesis rate, and concentrations of Na+ and K+ in leaves. The principal component analysis showed that shoot dry weight and K+/Na+ ratio contribute the most to salt tolerance among the garlic accessions. As a result, salt-tolerant and sensitive accessions were grouped based on these two parameters. Furthermore, to investigate the molecular mechanisms in garlic in response to salinity, the transcriptomes of leaves and roots between salt-tolerant and salt-sensitive garlic accessions were compared. Approximately 1.5 billion read pairs were obtained from 24 libraries generated from the leaves and roots of the salt-tolerant and salt-sensitive garlic accessions. A total of 47,509 genes were identified by mapping the cleaned reads to the garlic reference genome. Statistical analysis indicated that 1282 and 1068 genes were upregulated solely in the tolerant leaves and roots, whereas 1505 and 1203 genes were downregulated exclusively in the tolerant leaves and roots after NaCl treatment, respectively. Functional categorization of these genes revealed their involvement in a variety of biological processes. Several genes important for carotenoid biosynthesis, auxin signaling, and K+ transport were strongly altered in roots by NaCl treatment and could be candidate genes for garlic salt tolerance improvement.

scholarly journals Detecting Salt Tolerance in Doubled Haploid Wheat Lines

Agronomy ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 211 ◽  
Author(s):  
Al-Ashkar ◽  
Alderfasi ◽  
El-Hendawy ◽  
Al-Suhaibani ◽  
El-Kafafi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Doubled Haploid ◽  
Dry Weight ◽  
Shoot Length ◽  
Salt Tolerant ◽  
Breeding Programs ◽  
Shoot Dry Weight ◽  
Wheat Lines

Improving salt tolerance of genotypes requires a source of genetic variation and multiple accurate selection criteria for discriminating their salt tolerance. A combination of morpho-physiological and biochemical parameters and multivariate analysis was used to detect salt tolerance variation in 15 wheat lines developed by doubled haploid (DHL) technique. They were then compared with the salt-tolerant check cultivar Sakha 93. Salinity stress was investigated at three salinity levels (0, 100, and 200 mM NaCl) for 25 days. Considerable genetic variation was observed for all traits, as was high heritability (>60%) and genetic gain (>20%). Principal component analysis indicated the ability of nine traits (root number, root length, root dry weight, shoot length, shoot dry weight, specific root length, relative water content, membrane stability index, and catalase) to identify differences in salinity tolerance among lines. Three traits (shoot length, shoot dry weight, and catalase) were indicative of salt-tolerance, indicating their importance in improving and evaluating salt tolerant genotypes for breeding programs. The salinity tolerance membership index based on these three traits classified one new line (DHL21) and the check cultivar (Sakha 93) as highly salt-tolerant, DHL25, DHL26, DHL2, DHL11, and DHL5 as tolerant, and DHL23 and DHL12 as intermediate. Discriminant function analysis and MANOVA suggested differences among the five groups of tolerance. Among the donor genotypes, Sakha 93 remained the donor of choice for improving salinity tolerance during the seedling stage. The tolerated lines (DHL21, DHL25, DHL26, DHL2, DHL11, and DHL5) could be also recommended as useful and novel genetic resources for improving salinity tolerance of wheat in breeding programs.

Assessment of salt tolerance and analysis of the salt tolerance gene Ncl in Indonesian soybean germplasm

2019 ◽  
Vol 17 (03) ◽  
pp. 265-271
Author(s):  
D. Cao ◽  
Y. L. Yan ◽  
D. H. Xu
Keyword(s):  
Salt Tolerance ◽  
Dry Weight ◽  
Salt Tolerant ◽  
Soil Medium ◽  
Spad Value ◽  
Shoot Dry Weight ◽  
Leaf Chlorophyll ◽  
Tolerance Gene ◽  
Salt Tolerance Gene ◽  
Hydroponic Conditions

AbstractSoybean [Glycine max (L.) Merr.] is one of the most important legume crops in the world. However, soybean grain yield is extensively affected by environmental stresses such as soil salinity. In this study, we evaluated the germplasm of 51 Indonesian soybean accessions for salt tolerance to identify salt-tolerant germplasms for possible use in breeding for soybean salt tolerance. Based on experiments under hydroponic conditions, adding 100 mM of NaCl to a 1/2 concentration of Hoagland and Arnon solution, several Indonesian soybean germplasms, such as Java 7, Seputih Raman, Tambora, Ringgit (JP 30217), Sinyonya (early) and Sinyonya (late) were identified as salt-tolerant in terms of salt tolerance rate (STR) and leaf chlorophyll content (SPAD value) taken with the Konica Minolta SPAD-502 chlorophyll meter. The selected salt-tolerant germplasms were further evaluated under soil medium cultivation in pots irrigated with 100 mM NaCl for around 5 weeks. The six selected soybean germplasms again showed higher salt tolerance in terms of SPAD, STR and shoot dry weight. Expression analysis of the salt tolerance gene Ncl revealed a significant positive correlation between Ncl expression and salt tolerance, suggesting that Ncl is essential for salt tolerance in the Indonesian soybean germplasms we tested. The salt-tolerant Indonesian soybean germplasms identified in this study could be used in local soybean breeding practices for the improvement of salt tolerance.

scholarly journals Identification and Characterization of Wheat Germplasm for Salt Tolerance

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Xiaoyan Quan ◽  
Xiaoli Liang ◽  
Hongmei Li ◽  
Chunjuan Xie ◽  
Wenxing He ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Redox Homeostasis ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Limiting Factors ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Shoot Height ◽  
Wheat Genotypes

Salinity is one of the limiting factors of wheat production worldwide. A total of 334 internationally derived wheat genotypes were employed to identify new germplasm resources for salt tolerance breeding. Salt stress caused 39, 49, 58, 55, 21 and 39% reductions in shoot dry weight (SDW), root dry weight (RDW), shoot fresh weight (SFW), root fresh weight (RFW), shoot height (SH) and root length (RL) of wheat, respectively, compared with the control condition at the seedling stage. The wheat genotypes showed a wide genetic and tissue diversity for the determined characteristics in response to salt stress. Finally, 12 wheat genotypes were identified as salt-tolerant through a combination of one-factor (more emphasis on the biomass yield) and multifactor analysis. In general, greater accumulation of osmotic substances, efficient use of soluble sugars, lower Na+/K+ and a higher-efficiency antioxidative system contribute to better growth in the tolerant genotypes under salt stress. In other words, the tolerant genotypes are capable of maintaining stable osmotic potential and ion and redox homeostasis and providing more energy and materials for root growth. The identified genotypes with higher salt tolerance could be useful for developing new salt-tolerant wheat cultivars as well as in further studies to underline the genetic mechanisms of salt tolerance in wheat.

scholarly journals Composition and Variability of the Essential Oil of the Flowers of Lavandula stoechas from Various Geographical Sources

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Salvatore La Bella ◽  
Teresa Tuttolomondo ◽  
Giacomo Dugo ◽  
Giuseppe Ruberto ◽  
Claudio Leto ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Statistical Method ◽  
Principal Component ◽  
Dry Weight ◽  
Component Analysis ◽  
Percentage Content ◽  
Lavandula Stoechas

Samples of flowers of wild Lavandula stoechas L. spp. stoechas populations were collected in three areas of Sicily (Italy) and were characterized in agronomic and chemical terms. Essential oil (EO) was extracted by hydrodistillation and analyzed by GC-FID and GC-MS. GC-FID and GC-MS analyses permitted identification of 89 compounds from the EO. The samples were separated into 3 groups using PCA (Principal Component Analysis) statistical method, with reference to the chemical composition of the EO. All three Sicilian populations of lavender were identified as the fenchone chemotype with percentage content ranged between 52.8–71.1%. The population of Partinico showed the highest dry weight of flowers per plant (221.3 g), but the lowest EO yield (0.37%). The essential oils of the three wild Sicilian populations of L. stoechas L. spp. stoechas showed a greater chemical differentiation than those obtained from other Mediterranean areas.

scholarly journals Effects of salinity stress on chickpea (Cicer arietinum L.) landraces during early growth stage

2017 ◽  
Vol 3 (7) ◽  
pp. 214 ◽  
Author(s):  
Seyoum Zawude ◽  
Diriba Shanko
Keyword(s):  
Salinity Stress ◽  
Root Length ◽  
Growth Stage ◽  
Dry Weight ◽  
Shoot Length ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Root Dry Weight ◽  
Shoot Dry Weight

<p class="abstract"><strong>Background:</strong> Salinity is one of the most serious abiotic stresses for crop plant growth. Chickpea grows under a wide range of climatic conditions and highly sensitive to salt stress. To determine the most tolerant genotype to salinity stress, an experiment was done as factorial form under completely block design (CRD) with three replications.</p><p class="abstract"><strong>Methods:</strong> The experimental treatments were four NaCl salinity levels (0, 5dS/m, 10dS/m and 15dS/m) and five chickpea landraces (Dadi, Dido, Dida, Dimi and Soya).  </p><p class="abstract"><strong>Results:</strong> Results indicated that significant was observed in root length, shoot length, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, seedling shoot and root reduction traits in stress conditions. Dimi, Dido and Dadi were showed that high reduction in root length, shoot length, root length, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, seedling root and shoot in salinity conditions, respectively. The ANOVA for landraces and their interaction was found to be highly significant at (p&lt;0.001) and (p&lt;0.05) with all parameters. Landraces Dimi, Dido and Dadi were found salt tolerant but Soya was highly salt sensitive during seedling growth stage. Shoot dry weight had the most positive and significant correlation with root dry weight (r =0.987**). Seedling shoot reduction depicted a negative and significant correlation with total dry matter (r =-0.734**).</p><strong>Conclusions:</strong> This study indicated that developing genetic variability by identifying salt tolerant landrace is one of the appropriate strategies used to overcome salinity problem in arid and semi-arid areas.

scholarly journals Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

2016 ◽  
Vol 44 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Behrooz SARABI ◽  
Sahebali BOLANDNAZAR ◽  
Nasser GHADERI ◽  
Seyed Jalal TABATABAEI
Keyword(s):  
Principal Component Analysis ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Seedling Growth ◽  
Principal Component ◽  
Component Analysis ◽  
Growth Stages ◽  
Tolerance Index ◽  
Plant Seed ◽  
Salt Tolerance Index

Although salt stress affects all growth stages of a plant, seed germination and seedling growth stages are known to be more sensitive for most plant species. The current research was carried out to find the most effective indicators when screening melons for salt tolerance. For this purpose, a factorial experiment as a completely randomized design was performed in order to investigate the effects of salinity (0, 20, 40, 60 and 80 mM NaCl) on seed germination parameters, hydrogen peroxide content, lipid peroxidation and some antioxidant enzymes (guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase) in five Iranian melon landraces and one melon cultivar ‘Galia’ F1 at early seedling growth stage. The ‘Galia’ F1 was included for comparison as a salt-tolerant cultivar. The data collected from all salinity treatments across six landraces were used for analysis of variance and principal component analysis (PCA) using SPSS software as well as for obtaining Pearson’s correlation coefficients (r) among all physiological parameters. Obtained results indicated that averaged all landraces across all treatments, increased NaCl significantly affected all the parameters measured. In all landraces, salt tolerance index (STI) decreased as the salt concentrations increased. The principal component analysis revealed variations among the traits and determined four main factors that explained 97.53% of the total variance. Simple correlation coefficient analyses showed the existence of significant positive and negative correlations among characteristics. Our study suggests that the salt tolerance index and principal component analysis can be used as accurate and easy indicators when screening melons for salt tolerance.

scholarly journals Growth, Fruit Yield, and Ion Concentration in Tomato Genotypes after Pre- and Post-emergence Salt Treatments

1993 ◽  
Vol 118 (5) ◽  
pp. 655-660 ◽  
Author(s):  
M.C. Bolarín ◽  
F. Pérez-Alfocea ◽  
E.A. Cano ◽  
M.T. Estañ ◽  
M. Caro
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Growth Stage ◽  
Dry Weight ◽  
Fruit Yield ◽  
Ion Concentration ◽  
Growth Stages ◽  
Salt Tolerant ◽  
Shoot Dry Weight ◽  
Advanced Stages

The effects of increasing salinity on dry weight and ion concentration of shoots at various growth stages and on fruit yield in four tomato (Lycopersicon esculentum Mill.) genotypes were assessed. The salt treatments (35, 70, and 140 mm NaCl) were applied pre-emergence (seed sowing) (pre-E) and post-emergence (four-leaf stage) (post-E) and maintained during plant growth. Genotype salt tolerance, measured as shoot dry weight in response to increases in salt concentration, varied depending on plant growth stage and salt application time. When salt was applied pre-E, salt tolerance increased with plant age, whereas when applied post-E, 45-day-old plants were the most salt tolerant. Mature plants were similarly salt tolerant independent of the growth stage at which the salt treatments began. However, fruit yield of all genotypes was higher when salt was applied pre-E than post-E. Shoot dry weight decreased as shoot Cl and Na ion concentrations increased. During early growth stages, pre-E salt-treated plants had the highest Cl-and Na+ concentrations and the lowest shoot dry weights. However, at the advanced stages, shoot Cl- and Na Concentrations were equal for both salt application times. These results show that the plants must adapt to salinity during a period that allows them to develop a mechanism to regulate internal Cl- and Na+ concentrations and, thus, grow under high salinity.

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