scholarly journals Accumulation of cadmium and zinc in barley regenerants on a provocative soil background with cadmium

2021 ◽  
Vol 182 (4) ◽  
pp. 117-125
Author(s):  
O. N. Shupletsova ◽  
E. V. Tovstik
Keyword(s):  
Spring Barley ◽  
Toxic Metal ◽  
Cadmium Accumulation ◽  
Soil Conditions ◽  
Original Form ◽  
Negative Effect ◽  
Somaclonal Variability ◽  
Normal Soil ◽  
Toxic Ions

Background. An effective way to obtain barley (Hordeum vulgare L.) genotypes stress-tolerant to cadmium, with a low level of toxic ion accumulation in grain, is the selection of cells in selective in vitro systems, based on somaclonal variability, which promotes the formation of specific and nonspecific adaptive mechanisms.Materials and methods. The object of the study was cv. ‘999-93’, developed by selection from a hybrid combination of spring barley (Luly × Conrad) × 2867-80, and its regenerated forms in seed reproductions of the 3–5th generation, induced in the process of cell selection on media with cadmium, aluminum and polyethylene glycol. The plants were grown under normal soil conditions and against a provocative background for cadmium.Results. The contribution of the environment-forming activity in the roots of the studied genotypes to inactivation of toxic ions appeared insignificant. The total removal of cadmium by plants against a provocative background increased 22.5 times, reaching 5.8–10.3 mg/kg of dry phytomass when distributed among organs: roots (91.9–93.4%) > stems (5.9–7.8%) > grain (0.5–0.8%). The amount of toxic metal in grain increased 11 times in the original form and 2.8–6.8 times in regenerants. The negative effect of excess cadmium in the soil on the accumulation of zinc in barley was shown. There was no gradation in the importance of organs for zinc accumulation or any presence of functional barriers preventing this. Regenerants induced on selective media with cadmium had the greatest adaptive advantages to stress: pronounced barrier functions of roots, minimal accumulation of toxic ions in aerial organs, and high seed productivity (they exceeded the original genotype by 35.5%). Adaptive reactions associated with the limitation of cadmium accumulation in plant tissues of regenerants, obtained by in vitro selections with aluminum and an osmotic, were shown to be weak.

scholarly journals Obtaining barley genotypes in selective in vitro systems with complex resistance to soil stress factors

2020 ◽  
Author(s):  
O.N. Shupletsova ◽  
Keyword(s):  
Spring Barley ◽  
Stress Factors ◽  
Soil Conditions ◽  
Regenerated Plants ◽  
Soil Stress ◽  
In Vitro Systems ◽  
High Level ◽  
Barley Genotypes ◽  
Callus Lines

The aim of the work was to develop selective in vitro systems and obtain on their basis the initial breeding material of spring barley adapted to adverse soil conditions – increased acidity, toxicity of aluminum and heavy metals, drought. In the process of research, optimal patterns for selecting callus lines on selective media using various combinations of stress factors were identified: Al3 + (20–40 mg/l), H+ (4.0–6.0 pH units), Cd2 + (10-20 mg/l), Mn2 + (100–250 mg/l) and osmotic (10–20 % polyethyleneglycol). In the proposed in vitro selective systems, more than a thousand regenerated plants were obtained. Varieties created on the basis of regenerants exceed the standard in yield, have high productive tillering (29.0–67.5 % higher than the standard) and dense spike (4.5–6.6 % higher than the standard). Their advantage is due to resistance to lodging, a high level of survival, germination and environment-forming activity of the root system.

scholarly journals Effects of nonspecific resistance in barley genotypes obtained by cell selection

2021 ◽  
Vol 181 (4) ◽  
pp. 192-199
Author(s):  
O. N. Shupletsova ◽  
S. Yu. Ogorodnikova ◽  
Ya. I. Nazarova
Keyword(s):  
Oxidative Stress ◽  
Root System ◽  
Spring Barley ◽  
Cadmium Toxicity ◽  
Soil Conditions ◽  
Cell Selection ◽  
Water Deficiency ◽  
Sources Of Resistance ◽  
Nonspecific Resistance

An effective way to increase the genetic diversity of plants and create sources of resistance to edaphic stressors is the selection of cells under selective in vitro conditions based on somaclonal variation. The use of somaclones (regenerant forms) of barley with resistance to increased acidity, ionic toxicity of aluminum, heavy metals and drought is promising in the northeast of the Non-Black-Soil Zone of Russia. Development of regenerants with integrated resistance to soil stressors due to specific and nonspecific mechanisms is a relevant trend.The target research material was a hybrid combination of spring barley (Luly × Conrad) × 2867-80 and its regenerant forms obtained as a result of cell selection with stressors of various nature. Plants were grown under ordinary soil conditions and on provocative backgrounds with aluminum and cadmium. The productive traits of plants, the symptoms of oxidative stress, and the environment-forming activity of the root system were evaluated.In regenerated plants obtained in callus culture on selective in vitro media with aluminum or water deficiency, an increased ability of the root system to alkalize the medium in the rhizosphere zone (by 0.2–0.5 pH units) when grown on alumina soil was revealed. Against stressful soil backgrounds, regenerative lines induced on in vitro media with cadmium or aluminum were observed to have a low manifestation of oxidative stress symptoms, tested by the intensity of lipid peroxidation and the content of photosynthetic pigments in the leaves, which provided these genotypes with higher seed productivity (1.5–3.6 times in the number of grains, and 1.5– 3.0 times in the grain weight per plant) and adaptive advantages compared to the original genotype and regenerants induced on an in vitro medium with water deficiency. It is concluded that complex resistance to soil aluminum and cadmium toxicity is formed in barley regenerants obtained in the process of cell selection on in vitro media with any of these metals. 

scholarly journals Effect of low molecular weigt osmogenic substanses on the induction of embryogenesis in spring barley anther culture in vitro

1970 ◽  
pp. 164-167
Author(s):  
O. V. Bilynska
Keyword(s):  
Plant Regeneration ◽  
Osmotic Pressure ◽  
Anther Culture ◽  
Spring Barley ◽  
Hordeum Vulgare L ◽  
Culture In Vitro ◽  
Negative Effect ◽  
Embryoid Formation ◽  
The Impact

Aim. Osmotic pressure of nutrient medium is known to be an important factor for morphogenesis in plant cell, tissue and organ culture in vitro. The investigations was aimed to elucidate the effect of maltose concentration and to evaluate the impact of mannitol add on callus, embryoid formation and plant regeneration in spring barley anther culture in vitro. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media contained N6 macro-, MS micronutrients, organic supplements, maltose (0−9.0 %) and 0.1 M mannitol. Results. A decrease in maltose concentration from 9 to 6 % had a strong negative effect on these processes. At the same time, addition of 0.1 M mannitol to medium containing 6 % maltose promoted sufficiently increase the efficiency of embryoid formation and plant regeneration. Conclusions. Medium osmotic pressure mainly determined by a high maltose concentration played an important role in spring barley haploid production. Decrease in maltose content could be compensated by addition of mannitol, which is osmotic substance with low metabolic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, embryo formation, plant regeneration.

The depressing (negative) effect of oestradiol benzoate on the in vitro secretion of LH and FSH by the pituitary gland of the LRH-pretreated long-term ovariectomized rat changes into the augmentative (positive) effect after discontinuation of the LRH-pretreatment

1985 ◽  
Vol 110 (3) ◽  
pp. 329-337 ◽  
Author(s):  
G. A. Schuiling ◽  
H. Moes ◽  
T. R. Koiter
Keyword(s):  
Depressing Effect ◽  
Ovx Rats ◽  
Gonadotrophin Secretion ◽  
Oestradiol Benzoate ◽  
Negative Effect ◽  
Positive Effect ◽  
Perifusion System

Abstract. The effect of pretreatment in vivo with oestradiol benzoate on in vitro secretion of LH and FSH was studied in long-term ovariectomized (OVX) rats both at the end of a 5-day continuous in vivo pretreatment with LRH and 4-days after cessation of such LRH pretreatment. Rats were on day 0 sc implanted with osmotic minipumps which released LRH at the rate of 250 ng/h. Control rats were implanted with a piece of silicone elastomer with the dimensions of a minipump. On days 2 and 4 the rats were injected with either 3 μg EB or with oil. On day 5 part of the rats were decapitated and the in vitro autonomous (i.e. non-LRH-stimulated) and 'supra-maximally' LRHstimulated release of LH and FSH was studied using a perifusion system. From other rats the minipumps were removed on day 5 and perifusion was performed on day 9. On the 5th day of the in vivo LRH pretreatment the pituitary LH/FSH stores were partially depleted; the pituitaries of the EB-treated rats more so than those of the oil-injected rats. EB alone had no significant effect on the content of the pituitary LH- and FSH stores. On day 9, i.e. 4 days after removal of the minipumps, the pituitary LH and FSH contents had increased in both the oil- and the EB injected rats, but had not yet recovered to control values. In rats not subjected to the 5-days pretreatment with LRH EB had a positive effect on the supra-maximally LRH-stimulated secretion of LH and FSH as well as on the non-stimulated secretion of LH. EB had no effect on the non-stimulated secretion of FSH. After 5 days of in vivo pretreatment with LRH only, the in vitro non-stimulated and supra-maximally LRH-stimulated secretion of both LH and FSH were strongly impaired, the effect correlating well with the LRH-induced depletion of the pituitary LH/FSH stores. In such LRH-pretreated rats EB had on day 5 a negative effect on the (already depressed) LRH-stimulated secretion of LH (not on that of FSH). EB had no effect on the non-stimulated LH/FSH secretion. It could be demonstrated that the negative effect of the combined LRH/EB pretreatment was mainly due to the depressing effect of this treatment on the pituitary LH and FSH stores: the effect of oestradiol on the pituitary LRH-responsiveness (release as related to pituitary gonadotrophin content) remained positive. In LRH-pretreated rats, however, this positive effect of EB was smaller than in rats not pretreated with LRH. Four days after removal of the minipumps there was again a positive effect of EB on the LRH-stimulated secretion of LH and FSH as well as on the non-stimulated secretion of LH. The positive effect of EB on the pituitary LRH-responsiveness was as strong as in rats which had not been exposed to exogenous LRH. The non-stimulated secretion of FSH was again not affected by EB. The results demonstrate that the effect of EB on the oestrogen-sensitive components of gonadotrophin secretion consists of two components: an effect on the pituitary LRH-responsiveness proper, and an effect on the pituitary LH/FSH stores. The magnitude of the effect of EB on the LRH-responsiveness is LRH dependent: it is very weak (almost zero) in LRH-pretreated rats, but strong in rats not exposed to LRH as well as in rats of which the LRH-pretreatment was stopped 4 days previously. Similarly, the effect of EB on the pituitary LH and FSH stores is LRH-dependent: in the absence of LRH, EB has no influence on the contents of these stores, but EB can potentiate the depleting effect of LRH on the LH/FSH-stores. Also this effect disappear after cessation of the LRH-pretreatment.

INFLUENCE OF DICARBOXYLIC ACIDS ON SOWING QUALITY OF SPRING BARLEY

1970 ◽  
pp. 22-25
Author(s):  
N. N. Loy ◽  
S. N. Gulina
Keyword(s):  
Organic Acids ◽  
Spring Barley ◽  
Dose Range ◽  
Dicarboxylic Acids ◽  
Rotary Machine ◽  
Working Solution ◽  
Negative Effect ◽  
Sprouted Seeds

The effect of presowing seed treatment on various concentrations of dicarboxylic (organic) acids on the sowing characteristics of spring barley has been studied. Seeds were treated with organic acids obtained by exposing cuttings to the radiation with a dose of 100 kGy and consequent hydrolysis, in concentrations: 1•10-7 %; 1•10-9; 1•10-11; 1•10-13 and 1•10-15 % on a laboratory rotary machine RVO-64 for one day before laying for germination. Distilled water was used for the control case. The rate of application of the working solution calculated as 10 liters / ton of seeds. Seeds were germinated in filter paper rolls in accordance with GOST 12038-84 requirements. The temperature was maintained at +24 ° C in the thermostat where the glasses with rolls were placed. For determination of germinative power and laboratory germination the sprouted seeds were evaluated after three and seven days, respectively. In laboratory experiments it was established that the treatment of barley seeds of varieties Zazersky 85, Nur and Vladimir with organic acids (OK) in different concentrations had both a stimulating and a negative effect. On the Zazersky 85 variety, in variants with acid concentrations of 1•10-9 and 1•10-11, an increase in germination energy (EP) by 2-4% and a significant decrease (by 3-4%) of laboratory germination (LV) of barley seeds were noted. On the Nur variety, the increase in EP was observed at 4% (concentration 1•10-11), LV and seed growth force (CPC) by 2-7% at a concentration of 1•10-7 and in the dose range 1•10-11 - 1•10-14 compared to the control values. On the grade of Vladimir, an increase in EP, LV, and CPC was found to increase by 1-6% at concentrations OK 1•10-7 and 1•10-13. It was shown that the treatment of seeds with acids led to an increase in the length of the germ in all studied varieties (by 3-9%) and dry biomass of 7-day-old seedlings - by 3-6%. Consequently, the treatment of seeds with a mixture of dicarboxylic acids has a stimulating effect on the sowing quality of spring barley.

Associated microflora of medicinal ferns: biotechnological potentials and possible applications

2016 ◽  
Vol 5 (03) ◽  
pp. 4927 ◽  
Author(s):  
Shubhi Srivastava ◽  
Paul A. K.
Keyword(s):  
Secondary Metabolites ◽  
Growth Promotion ◽  
Biological Activities ◽  
Hydrolytic Enzymes ◽  
In Vitro Production ◽  
Wide Range ◽  
Negative Effect ◽  
Bacteria And Fungi

Plant associated microorganisms that colonize the upper and internal tissues of roots, stems, leaves and flowers of healthy plants without causing any visible harmful or negative effect on their host. Diversity of microbes have been extensively studied in a wide variety of vascular plants and shown to promote plant establishment, growth and development and impart resistance against pathogenic infections. Ferns and their associated microbes have also attracted the attention of the scientific communities as sources of novel bioactive secondary metabolites. The ferns and fern alleles, which are well adapted to diverse environmental conditions, produce various secondary metabolites such as flavonoids, steroids, alkaloids, phenols, triterpenoid compounds, variety of amino acids and fatty acids along with some unique metabolites as adaptive features and are traditionally used for human health and medicine. In this review attention has been focused to prepare a comprehensive account of ethnomedicinal properties of some common ferns and fern alleles. Association of bacteria and fungi in the rhizosphere, phyllosphere and endosphere of these medicinally important ferns and their interaction with the host plant has been emphasized keeping in view their possible biotechnological potentials and applications. The processes of host-microbe interaction leading to establishment and colonization of endophytes are less-well characterized in comparison to rhizospheric and phyllospheric microflora. However, the endophytes are possessing same characteristics as rhizospheric and phyllospheric to stimulate the in vivo synthesis as well as in vitro production of secondary metabolites with a wide range of biological activities such as plant growth promotion by production of phytohormones, siderophores, fixation of nitrogen, and phosphate solubilization. Synthesis of pharmaceutically important products such as anticancer compounds, antioxidants, antimicrobials, antiviral substances and hydrolytic enzymes could be some of the promising areas of research and commercial exploitation.

scholarly journals Accuracy of Guided Implant Surgery in the Edentulous Jaw Using Desktop 3D-Printed Mucosal Supported Guides

2021 ◽  
Vol 10 (3) ◽  
pp. 391
Author(s):  
Rani D’haese ◽  
Tom Vrombaut ◽  
Geert Hommez ◽  
Hugo De Bruyn ◽  
Stefan Vandeweghe
Keyword(s):  
In Vitro Study ◽  
Angular Deviation ◽  
Implant Surgery ◽  
Implant Position ◽  
Surgical Guides ◽  
Negative Effect ◽  
Bone Level ◽  
3D Printed ◽  
Implant Alignment

Purpose: The aim of this in vitro study is to evaluate the accuracy of implant position using mucosal supported surgical guides, produced by a desktop 3D printer. Methods: Ninety implants (Bone Level Roxolid, 4.1 mm × 10 mm, Straumann, Villerat, Switzerland) were placed in fifteen mandibular casts (Bonemodels, Castellón de la Plana, Spain). A mucosa-supported guide was designed and printed for each of the fifteen casts. After placement of the implants, the location was assessed by scanning the cast and scan bodies with an intra-oral scanner (Primescan®, Dentsply Sirona, York, PA, USA). Two comparisons were performed: one with the mucosa as a reference, and one where only the implants were aligned. Angular, coronal and apical deviations were measured. Results: The mean implant angular deviation for tissue and implant alignment were 3.25° (SD 1.69°) and 2.39° (SD 1.42°) respectively, the coronal deviation 0.82 mm (SD 0.43 mm) and 0.45 mm (SD 0.31 mm) and the apical deviation 0.99 mm (SD 0.45 mm) and 0.71 mm (SD 0.43 mm). All three variables were significantly different between the tissue and implant alignment (p < 0.001). Conclusion: Based on the results of this study, we conclude that guided implant surgery using desktop 3D printed mucosa-supported guides has a clinically acceptable level of accuracy. The resilience of the mucosa has a negative effect on the guide stability and increases the deviation in implant position.

scholarly journals Restoration of Silencing in Saccharomyces cerevisiae by Tethering of a Novel Sir2-Interacting Protein, Esc8

Genetics ◽  
2002 ◽  
Vol 162 (2) ◽  
pp. 633-645 ◽  
Author(s):  
Guido Cuperus ◽  
David Shore
Keyword(s):  
Protein A ◽  
Dominant Negative ◽  
Class I ◽  
Dominant Negative Effect ◽  
Rna Pol Ii ◽  
Interacting Protein ◽  
Nucleosome Remodeling ◽  
Negative Effect ◽  
Close Homolog

Abstract We previously described two classes of SIR2 mutations specifically defective in either telomeric/HM silencing (class I) or rDNA silencing (class II) in S. cerevisiae. Here we report the identification of genes whose protein products, when either overexpressed or directly tethered to the locus in question, can establish silencing in SIR2 class I mutants. Elevated dosage of SCS2, previously implicated as a regulator of both inositol biosynthesis and telomeric silencing, suppressed the dominant-negative effect of a SIR2-143 mutation. In a genetic screen for proteins that restore silencing when tethered to a telomere, we isolated ESC2 and an uncharacterized gene, (YOL017w), which we call ESC8. Both Esc2p and Esc8p interact with Sir2p in two-hybrid assays, and the Esc8p-Sir2 interaction is detected in vitro. Interestingly, Esc8p has a single close homolog in yeast, the ISW1-complex factor Ioc3p, and has also been copurified with Isw1p, raising the possibility that Esc8p is a component of an Isw1p-containing nucleosome remodeling complex. Whereas esc2 and esc8 deletion mutants alone have only marginal silencing defects, cells lacking Isw1p show a strong silencing defect at HMR but not at telomeres. Finally, we show that Esc8p interacts with the Gal11 protein, a component of the RNA pol II mediator complex.

scholarly journals Dominant Negative Mutants Implicate STAT5 in Myeloid Cell Proliferation and Neutrophil Differentiation

Blood ◽  
1999 ◽  
Vol 93 (12) ◽  
pp. 4154-4166 ◽  
Author(s):  
Robert L. Ilaria ◽  
Robert G. Hawley ◽  
Richard A. Van Etten
Keyword(s):  
Dna Binding ◽  
Transcriptional Activation ◽  
Dominant Negative ◽  
Dominant Negative Effect ◽  
Cytokine Signaling ◽  
Transactivation Domain ◽  
Negative Effects ◽  
Murine Bone Marrow ◽  
Negative Effect

Abstract STAT5 is a member of the signal transducers and activation of transcription (STAT) family of latent transcription factors activated in a variety of cytokine signaling pathways. We introduced alanine substitution mutations in highly conserved regions of murine STAT5A and studied the mutants for dimerization, DNA binding, transactivation, and dominant negative effects on erythropoietin-induced STAT5-dependent transcriptional activation. The mutations included two near the amino-terminus (W255KR→AAA and R290QQ→AAA), two in the DNA-binding domain (E437E→AA and V466VV→AAA), and a carboxy-terminal truncation of STAT5A (STAT5A/▵53C) analogous to a naturally occurring isoform of rat STAT5B. All of the STAT mutant proteins were tyrosine phosphorylated by JAK2 and heterodimerized with STAT5B except for the WKR mutant, suggesting an important role for this region in STAT5 for stabilizing dimerization. The WKR, EE, and VVV mutants had no detectable DNA-binding activity, and the WKR and VVV mutants, but not EE, were defective in transcriptional induction. The VVV mutant had a moderate dominant negative effect on erythropoietin-induced STAT5 transcriptional activation, which was likely due to the formation of heterodimers that are defective in DNA binding. Interestingly, the WKR mutant had a potent dominant negative effect, comparable to the transactivation domain deletion mutant, ▵53C. Stable expression of either the WKR or ▵53C STAT5 mutants in the murine myeloid cytokine-dependent cell line 32D inhibited both interleukin-3–dependent proliferation and granulocyte colony-stimulating factor (G-CSF)–dependent differentiation, without induction of apoptosis. Expression of these mutants in primary murine bone marrow inhibited G-CSF–dependent granulocyte colony formation in vitro. These results demonstrate that mutations in distinct regions of STAT5 exert dominant negative effects on cytokine signaling, likely through different mechanisms, and suggest a role for STAT5 in proliferation and differentiation of myeloid cells.

scholarly journals The Diagnostic Journey of a Patient with Prader–Willi-Like Syndrome and a Unique Homozygous SNURF-SNRPN Variant; Bio-Molecular Analysis and Review of the Literature

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 875
Author(s):  
Karlijn Pellikaan ◽  
Geeske M. van Woerden ◽  
Lotte Kleinendorst ◽  
Anna G. W. Rosenberg ◽  
Bernhard Horsthemke ◽  
...  
Keyword(s):  
Intellectual Disability ◽  
Single Nucleotide Polymorphism Array ◽  
Genetic Defect ◽  
Missense Variant ◽  
Dominant Negative ◽  
Dominant Negative Effect ◽  
Pituitary Hormone ◽  
Negative Effect

Prader–Willi syndrome (PWS) is a rare genetic condition characterized by hypotonia, intellectual disability, and hypothalamic dysfunction, causing pituitary hormone deficiencies and hyperphagia, ultimately leading to obesity. PWS is most often caused by the loss of expression of a cluster of genes on chromosome 15q11.2-13. Patients with Prader–Willi-like syndrome (PWLS) display features of the PWS phenotype without a classical PWS genetic defect. We describe a 46-year-old patient with PWLS, including hypotonia, intellectual disability, hyperphagia, and pituitary hormone deficiencies. Routine genetic tests for PWS were normal, but a homozygous missense variant NM_003097.3(SNRPN):c.193C>T, p.(Arg65Trp) was identified. Single nucleotide polymorphism array showed several large regions of homozygosity, caused by high-grade consanguinity between the parents. Our functional analysis, the ‘Pipeline for Rapid in silico, in vivo, in vitro Screening of Mutations’ (PRiSM) screen, showed that overexpression of SNRPN-p.Arg65Trp had a dominant negative effect, strongly suggesting pathogenicity. However, it could not be confirmed that the variant was responsible for the phenotype of the patient. In conclusion, we present a unique homozygous missense variant in SNURF-SNRPN in a patient with PWLS. We describe the diagnostic trajectory of this patient and the possible contributors to her phenotype in light of the current literature on the genotype–phenotype relationship in PWS.

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