scholarly journals Selection the optimal dose of application associated vaccine against rota-, coronavirus infection and colibacillosis in calves

Author(s):  
Y. P. Yaromchyk ◽  
P. А. Krasochko ◽  
P. P. Krasochko ◽  
V.M. Eremets ◽  
T.A. Skotnikova

The main rules for choosing the means of specific prophylactic against colibacillosis (escherichiosis) in cattle is the need to match the antigenic spectrum of the vaccine strains with epizootic strains isolated by diagnostic veteri-nary institutions from pathological material taken from dead calves. Only in this case one should expect high results of the preventive efficacy of the applied vac-cines. The design of vaccines based on pathogenicity factors of bacterias is the most promising direction in the development of new bioproducts of specific pre-vention of infectious diseases of farm animals. Researchs in the areas to choice the optimal doses and ratios of monocompo-nents, determining the optimal immunizing dose, and choosing a adjuvant is an im-portant part of research work on the creation of vaccines. We completed work to establish the optimal dose when using the associated vaccine against rota-, coronavirus infection and colibacillosis of cattle by vaccinat-ing cows in different doses of the test vaccine. Subsequently, serological studies of the blood of animals were carried out, according to the results of which the indica-tors of the best immune response were determined by comparing the established levels of biosynthesis of specific antibodies from animals of the experimental and control groups. A number of indicators of possible reactogenicity of the tested vac-cine were also studied. When conducting studies of blood serum of cows, the indi-rect hemagglutination reaction and agglutination reaction were performed. Serolog-ical blood tests carried out were accompanied by the necessary controls to ensure the reliability of the results. According to the results of studies of blood serum of cows in the experimental groups, immunization of cows with an associated vaccine against rota-, coronavirus infection and colibacillosis of cattle in different volumes led to a significant level of antiviral and antibacterial antibodies. The optimum dose for virus antigens with infectious titres from 7,0 lg and 5,5-TCI50/sm3 for vaccinated cows forms 1,5 sm3 for each viral monocomponents. The optimum dose for each vaccine strains E.coli F4, F5, Аtt25, F41 and 987P forms from 1,5-2,5 milliard bacterial cells for each bacterial monocomponents.

Author(s):  
V.V. Evstifeev ◽  
◽  
F.M. Khusainov ◽  
S.I. Yakovlev ◽  
G.I. Khusainova ◽  
...  

The article presents data on laboratory tests of a universal vaccine against chlamydia of farm animals for sterility, safety, antigenic activity and immunogenicity. The sterility of the new vaccine preparation was evaluated on nutrient media (MPA, MPB, MPPB and Saburo), the vaccine was harmless according to the results of bioassays on laboratory white mice and guinea pigs. The vaccine was found to be sterile and harmless to laboratory animals. Evaluation of antigenic activity was car-ried out on rabbits, the study lasted 195 days, the results were taken into account on the basis of data obtained during systematic serological studies of blood serum immunized with rabbits. It was found that the vaccine stimulates the immune system of laboratory animals to produce complemen-tary chlamydial antibodies. After 6.5 months after the administration of the drug, the titers of chla-mydial antibodies remained above the protective titer and were equal on average 1:60. Evaluation of the immunogenicity of the vaccine was carried out in an acute experiment on rabbits (females). Dur-ing the experiment, it was possible to establish the ability of the vaccine to induce the production of immunity in laboratory animals that can overcome chlamydial infection. Healthy rabbits were ob-tained from rabbits vaccinated and infected at the 3rd week of pregnancy, while mass abortions, stillbirths and the birth of non-viable offspring were noted in the control groups. According to the results of post-mortem dissection of animals from the experimental and control groups, it was found that vaccinated and further infected animals did not reveal any pathological changes in the internal organs, in contrast to the animals from the control groups.


Author(s):  
L. Ovchinnikova ◽  
E. Babich

In the Republic of Kazakhstan over the past decade positive changes have been observed in increasing the number of farm animals, increasing their productivity, and increasing the gross production of milk, meat and other products. At the same time the development of dairy cattle breeding is mainly due to the breeding of Black-and-White, Red Steppe, Simmental breeds and their intra-breed types as well as imported from abroad Holstein breed. The purpose of the research was to analyze ways to improve the intra-breed type Karatomar of Black-and-White breed in Northern Kazakhstan. Research work has been carried out on breeding animals such as Karatomar type cattle of Black-and-White breed in LLP “Experimental farm Zarechnoe” in the Kostanay district in the Kostanay region. The dynamics of growth and development indicators, reproductive traits of heifers of a new intra-breed type Karatomar of Black-and-White cattle have been studied. A comprehensive assessment of their economically useful traits in the first lactation, determined by the resistance of animals to climatic and forage conditions, the efficiency of breeding of animals of different genotypes has been conducted. It has been found that the replacement heifers – daughters of American breeding sires inherited precocity and showed higher growth energy after 6 months of age and continued to grow rapidly until 18 months. The advantage in metabolism was in the daughters of Holstein sires of the American selection Orbit 4078, and the increased immune status of the organism in the daughters of the sire of the domestic selection Yamal 975 as the most adapted to the conditions of Northern Kazakhstan. The best ability to increasing the milk yield, stable lactation activity, high milk productivity, gross content of dry matter and nonfat milk solids have been characterized by the daughters of sires of the American selection Orbit 4078 and Lowrider 4129. Analysis of economic indicators of milk production has been shown that from cows – daughters of sires Orbit 4078 and Lowrider 4129 for 305 days of lactation received 15,8 and 15,2 % more milk of base fat, the expenditures of EFU per 1 kg of production was lower by 10,2 and 5,9 %. A higher level of profitability has been observed in the group of daughters of the sires Orbit 4078 – 35,5 % and Lowrider 4129 – 33,1 %, which was 8,5 and 10,8 % higher than in the group of daughters of the sire Yamal 975.


Author(s):  
R C Segundo ◽  
P R English ◽  
G Burgess ◽  
S A Edwards ◽  
O MacPherson ◽  
...  

The role of stockmanship in relation to the wellbeing of farm animals has been emphasised in the UK Codes of Recommendations for the Welfare of Farm Livestock (MAFF, 1983). Moreover, previous research work has demonstrated important effects of good and bad stockmanship not only on welfare but also on growth, lactational and reproductive performance of pigs and other farm livestock (Hemsworth et al, 1987). There is a need, therefore, to establish the factors which motivate and demotivate stockpeople since the degree of job satisfaction is likely to have a considerable influence on the attitude and performance of stockpeople and on their empathy with the animals in their care. With this objective in mind, a questionnaire was designed to investigate the aspects which could have an influence on the job satisfaction of the stockpeople involved in pig production.


2021 ◽  
pp. 54-57
Author(s):  
Isra Khalil Mohammed Ali Saeed ◽  
Maha Hussein Mohammed Hamza ◽  
Hiba Hussein Ibrahim ◽  
Esmehan Elkheir Babeker ◽  
Ibrahim Ismail M.Abu ◽  
...  

An epidemic of new coronavirus 2019 (COVID-19) has emerged in China since December 2019. WHO declared it as a pandemic on March 2020 as it has spread worldwide. Several cases among neonate were observed with rst reported 36 hours after birth. Due to the possibility of the infection and the immature immune system of the neonate there should be preventive and control measures at Neonatal Intensive Care Units. According to WHO guideline and other published articles in COVID-19 in infants and neonate a technical working group including community physician and Pediatricians has put measures for clinical management, prevention and control of COVID-19 in neonates.


Microbiology ◽  
2006 ◽  
Vol 152 (6) ◽  
pp. 1621-1637 ◽  
Author(s):  
Malgorzata Adamczyk ◽  
Patrycja Dolowy ◽  
Michal Jonczyk ◽  
Christopher M. Thomas ◽  
Grazyna Jagura-Burdzy

The kfrA gene of the IncP-1 broad-host-range plasmids is the best-studied member of a growing gene family that shows strong linkage to the minimal replicon of many low-copy-number plasmids. KfrA is a DNA binding protein with a long, alpha-helical, coiled-coil tail. Studying IncP-1β plasmid R751, evidence is presented that kfrA and its downstream genes upf54.8 and upf54.4 were organized in a tricistronic operon (renamed here kfrA kfrB kfrC), expressed from autoregulated kfrAp, that was also repressed by KorA and KorB. KfrA, KfrB and KfrC interacted and may have formed a multi-protein complex. Inactivation of either kfrA or kfrB in R751 resulted in long-term accumulation of plasmid-negative bacteria, whereas wild-type R751 itself persisted without selection. Immunofluorescence studies showed that KfrAR751 formed plasmid-associated foci, and deletion of the C terminus of KfrA caused plasmid R751ΔC 2 kfrA foci to disperse and mislocalize. Thus, the KfrABC complex may be an important component in the organization and control of the plasmid clusters that seem to form the segregating unit in bacterial cells. The studied operon is therefore part of the set of functions needed for R751 to function as an efficient vehicle for maintenance and spread of genes in Gram-negative bacteria.


Author(s):  
Abhay Kumar Singh ◽  
Shabbiruddin

The need for a motor protection system can be well understood by the fact that motors are integral device in any of the present day industries. Malfunctioning or any other faults in motor can halt the functioning of such industries. This can cause huge financial losses. So an efficient motor protection system is necessary. The present research work deals with the application of Labview for motor protection system, which can constantly monitor and control, a large motor system. This paper presents a highly reliable approach towards protection of commonly used motors. Here we deal with different kinds of motor faults and detection of all these faults using NI LABVIEW™. The present paper will not only be helpful for industrial purposes but it can also be helpful for students to understand motor fault detection. The LABVIEW has been successfully applied to make an efficient motor protection system.


2021 ◽  
pp. 348-355
Author(s):  
Abderrazak Mazouak ◽  
Malika Tridane ◽  
Said Belaaouad

Digital technologies have come to shake up the traditional paradigm of learning, and to change the existing relationship of "know-teaching" to put in place a key concept of the time is "to accompany through digital tools Our intervention is part of a technical-pedagogical approach and will focus on an action research work in which we will try:First, to present on line an accessible digital device that facilitates and organizes project planning and provides means to govern and control the quality of administrative acts.Next, We will show the first results of the experimentation of this tool in our context of research represented by 355 directors of the secondary schools of the provincial delegation of Taza. Morocco, pointing out all the constraints and limitations that hampered its implementation.Finally, we confirm that the use of this device by our sampling has caused a triple effect on their pedagogical practices: the professionalization of strategic planning on the one hand, and the governance of resources and the rationalization of pedagogical decisions on the other hand and immediate evaluation and regulation in the third phase.


2020 ◽  
Vol 14 (6) ◽  
pp. 2219-2229
Author(s):  
A. Kindozandji ◽  
B.D. Datinon ◽  
K. Amevoin ◽  
A.N. Nondichao ◽  
A.I. Glitho ◽  
...  

Le niébé est l’une des légumineuses alimentaires la plus importante dans toutes les régions de l’Afrique Sub-saharienne. L’objectif de ce travail était d’étudier l’effet rémanent de Mavi Multinucléo polyhedrovirus (Mavi MNPV) après la récolte du niébé au champ. Le champ a été traité 5 fois au biopesticide Mavi MNPV à la fréquence de sept jours. Deux semaines après la récolte, deux plants ainsi que les graines de chaque traitement sur chaque unité parcellaire ayant reçu les traitements de Mavi MNPV de 107, 108 et 109 virions/ml ont été collectés et amenés au laboratoire avec le substrat de développement de la plante (sable). Aucune des formulations issues des substrats sable, racines, feuilles, tiges et graines n’a affecté la mortalité des larves L2 et L3 de Maruca vitrata et aucune différence significative n’a été observée entre les traitements et le témoin. Quels que soient les traitements, le taux d’émergence des adultes est élevé ; ce qui signifie que la variation de la concentration de Mavi MNPV ne conditionne pas sa présence dans le sol, les tiges, les feuilles, les racines et les graines. Le biopesticide Mavi MNPV n’est pas persistant dans la nature.Mots clés : Niébé, Mavi MNPV, Maruca vitrata, Effet rémanent   English Title: Study of the residual effect of Mavi MNPV in a cowpea field Cowpea is one of the most important food legumes in all regions of Sub-Saharan Africa. The objective of this research work was to study the persistence effect of Mavi Multinucleus polyhedrovirus (Mavi MNPV) after field harvest of the cowpea. The field was treated 5 times with Mavi MNPV biopesticide at seven days. Two weeks after harvest, two plants as well as the seeds from each treatment on each plot unit that received Mavi MNPV treatments of 107, 108 and 109 virions / ml were removed and brought to the laboratory with the plant development substrate (sand). None of the formulations from sand, root, leaf, stem and seed substrates did not affect the mortality of L2 and L3 larvae of Maruca vitrata and no significant difference was observed between treatments and control. Regardless of the treatments, the rate of emergence of adults is high; this means that the variation in the concentration of Mavi MNPV does not condition its presence in soil, stems, leaves, roots and seeds. Mavi MNPV biopesticide is not persistent in nature.Keywords: Cowpea, Mavi MNPV, Maruca vitrata, Residual effect.      


2021 ◽  
Vol 344 (5) ◽  
pp. 8-10
Author(s):  
S. Sh. Abdulmagomedov ◽  
A. Yu. Aliev ◽  
R. M. Bakrieva ◽  
E. A. Belkin

Relevance. Dagestan Republic in terms of natural and climatic characteristics is the most favorable for the of ixodid ticks - carriers of pathogens of blood-parasitic diseases of farm animals. In this regard, further improvement of the set of scientifically grounded control measures and the search for new promising chemotherapeutic drugs of the prevention and treatment of pyroplasmidosis of cattle are major problem of great national economic importance.Materials and methods. The studies were carried out in farms, unfavorable on pyroplasmidosis, in the conditions of Dagestan Republic. The object of the study was cattle, spontaneously invaded by various types of blood parasites. Experеmental and control groups in production experiments were selected according to the principle of analogues. In the first control group (n = 10) the drug was not used. The second (n = 10) was injected with the drug DAC 5% at a dose of 1 ml/20 kg (DV 2.5 mg/kg), intramuscularly, at the rate 5 ml per 100 kg of live weight. The animals of the third (n = 10) were injected with the injectable preparation forticarb 10% at a dose of 4 ml/100 kg (DV 4 mg/kg) of live weight, intramuscularly, once.Results. It was found that with a single intramuscular injection of forticarb at the rate 2 ml/50 kg of live weight, the temperature and parasitic reaction in the body of sick animals decreased in a very short time. Therapeutic efficacy in pyroplasmidosis of cattle was 90%.


2021 ◽  
Vol 14 (9) ◽  
pp. 2992-3000
Author(s):  
Amos Miningou ◽  
Vanessa Golane ◽  
Appolinaire Sy Traore ◽  
Hyacinthe Kambire

The decrease in soil fertility is the main cause of low crops yields in Burkina Faso. It is therefore necessary to make a supply of fertilizers to increase yields. This is the reason why during the 2015 rainy season from July to October, a study was conducted on sesame fertilization in Souri located in the Province du Mouhoun. The goal of our study is to fertilize plants with increasing doses of mineral fertilizers at different application dates on sesame. The objective is to determine the optimum dose and date of fertilizer application to increase the yields. For this purpose four NPK (14-23-14) fertilizer treatments have been experimented with following doses NPK0 (0kg.ha-1), NPK50 (50 kg.ha-1) NPK100 (100kg.ha-1) NPK150 (150 kg.ha-1) with three application dates (sowing, 15 DAS, 30 DAS) in Fisher’s completely randomized blocks with four replications in split-plot. The results showed that NPK doses had an effect on sesame throughout morphological development (height, number of ramifications, number of capsules) and yield performance. Yields have increased according to the doses applied in the following order: NPK0 (226 kg.ha-1) < NPK50 (348 kg.ha-1) <NPK100 (550 kg.ha-1) <NPK150 (593 kg.ha-1). Through economic calculations, the NPK100 dose with 550 kg.ha-1 yield can be chosen as the most cost-effective dose for producers. The application dates of NPK had various effects on sesame that emerged on the height and grain yield. The plants had the best performance on the date of application of the 15 DAS (475 kg.ha-1) followed by the application at planting time (450 kg.ha-1) and at the end the application on 30 DAS (375 kg.ha-1). Interaction between the doses and the dates of application of fertilizer is significant and should be considered in sesame. La baisse de la fertilité des sols est une cause des faibles rendements au Burkina Faso. Ainsi, il faudra faire de bons apports d’engrais pour augmenter les rendements. Dans ce cadre, une étude a été conduite de juillet à octobre 2015 à Souri dans la Province du Mouhoun. Il s’est agi d’apporter des doses croissantes de fumure minérale à différentes dates d’application sur le sésame. L’objectif est de déterminer la dose et la date d’application optimales de la fumure minérale. A cet effet quatre traitements de doses d’engrais NPK (14-23-14) : NPK0 (0 kg.ha-1), NPK50 (50 kg.ha-1), NPK100 (100kg.ha-1), NPK150 (150 kg.ha-1) ont été appliqués selon trois dates d’application (au semis, le 15ème JAS, le 30ème JAS) dans un dispositif expérimental en blocs de Fisher à 4 répétitions avec un arrangement en split-plot. Les résultats obtenus ont montré que les doses NPK ont un effet positif sur le sésame à travers son développement morphologique (sa hauteur, ses ramifications, ses capsules) et son rendement. Les rendements obtenus ont permis de classer les doses selon cet ordre croissant : NPK0 (266 kg.ha-1) <NPK50 (348 kg.ha-1) <NPK100 (550 kg.ha-1) <NPK150 (593kg.ha-1). L’évaluation économique effectuée sur la base du ratio valeur sur coût (RVC) a permis de retenir la dose NPK100 comme étant la plus rentable pour les producteurs. Les dates d’applications NPK sur le sésame ont également des effets visibles sur la hauteur et le rendement en grain. Le meilleur rendement est obtenu avec l’application du NPK 15 JAS (475 kg.ha-1) suivi de l’application au semis (450 kg.ha-1) et enfin de celle effectuée au 30ème JAS (375 kg.ha-1). L’interaction doses et dates d’application d’engrais NPK doit être considérée chez le sésame.


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