scholarly journals Polymorphism of the Ovine Calpastatin (CAST) Gene and its Association with Productive Traits in Nellore Sheep

2020 ◽  
Vol 10 (6) ◽  
Author(s):  
B. Ramadevi
2015 ◽  
Vol 95 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Paweł Urbański ◽  
Mariusz Pierzchała ◽  
Arkadiusz Terman ◽  
Marian Kamyczek ◽  
Marian Różycki ◽  
...  

Urbański, P., Pierzchała, M., Terman, A., Kamyczek, M., Różycki, M., Roszczyk, A. and Czarnik, U. 2015. The relationship between the polymorphism of the porcine CAST gene and productive traits in pigs. Can. J. Anim. Sci. 95: 361–367. The aim of the study was to characterize the polymorphism of the calpastatin gene identified with ApaLI, Hpy188I and PvuII restriction enzymes in two pig breeds and one line bred in Poland, and to evaluate the relationship between the CAST genotype and carcass traits. The analysis covered a total of 617 pigs of two breeds, Polish Landrace (185) and Polish Large White (216), and synthetic line L990 (216). All animals studied appeared to be monomorphic at two loci: CAST/ApaLI and CAST/Hpy188I, while three genotypes were observed at CAST/PvuII locus. Statistical analysis was carried out for each breed separately using the least square methods of the GLM procedure. The model included the effect of the CAST genotype, fixed effect of the RYR1 genotype and the effect of the sire. Because the RYR1 genotype could significantly modify the effect of other genes, the effect of the RYR1 genotype was included in the statistical model. The relationship between the polymorphism and several productive traits was identified in each of the study groups of pigs. Animals carrying the heterozygous genotype at this locus showed most extreme values for some of the traits tested. Our results suggest that the CAST /PvuII genotype might be utilized in the selection of valuable pig carcass traits, particularly weight and size of the loin.


BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Maulana M. Naji ◽  
Yuri T. Utsunomiya ◽  
Johann Sölkner ◽  
Benjamin D. Rosen ◽  
Gábor Mészáros

Abstract Background In evolutionary theory, divergence and speciation can arise from long periods of reproductive isolation, genetic mutation, selection and environmental adaptation. After divergence, alleles can either persist in their initial state (ancestral allele - AA), co-exist or be replaced by a mutated state (derived alleles -DA). In this study, we aligned whole genome sequences of individuals from the Bovinae subfamily to the cattle reference genome (ARS.UCD-1.2) for defining ancestral alleles necessary for selection signatures study. Results Accommodating independent divergent of each lineage from the initial ancestral state, AA were defined based on fixed alleles on at least two groups of yak, bison and gayal-gaur-banteng resulting in ~ 32.4 million variants. Using non-overlapping scanning windows of 10 Kb, we counted the AA observed within taurine and zebu cattle. We focused on the extreme points, regions with top 0. 1% (high count) and regions without any occurrence of AA (null count). High count regions preserved gene functions from ancestral states that are still beneficial in the current condition, while null counts regions were linked to mutated ones. For both cattle, high count regions were associated with basal lipid metabolism, essential for survival of various environmental pressures. Mutated regions were associated to productive traits in taurine, i.e. higher metabolism, cell development and behaviors and in immune response domain for zebu. Conclusions Our findings suggest that retaining and losing AA in some regions are varied and made it species-specific with possibility of overlapping as it depends on the selective pressure they had to experience.


2015 ◽  
Vol 14 (2) ◽  
pp. 5310-5319 ◽  
Author(s):  
L.N. Silva ◽  
E. Gasparino ◽  
R.A.A. Torres Júnior ◽  
K. Euclides Filho ◽  
L.O.C. Silva ◽  
...  

Author(s):  
A. Balnikov ◽  
I. Gridyushko ◽  
Yu. Kazutova ◽  
M. Mikhailova ◽  
E. Romanishko

Purpose: evaluation of pigs Yorkshire rocks and Landraz on PRKAG3, MC4R and MyOD1 markers.Materials and methods. The paper presents the analysis of the results of genetic testing, and determines the frequency of alleles and genotypes of Yorkshire and Landrace breeds at the State Enterprise ZhodinoAgroPlemElita" of the Minsk region of the Republic of Belarus by the genes-markers of productive traits: PRKAG3, MC4R and MYOD1. Zootechnical and molecular genetic methods were used in the research (PCR/PDRF). Snapshot analysis was developed for the genotyping of pigs to detect polymorphic markers of meat-feeding productivity of pigs on the Genas PRKAG3, MyOD1, MC4R. At the first stage, there was an assessment of tribal young (173 heads) on its own productivity in terms of indicators: the age of achieving the living mass of 100 kg, the average daily increase (d) from birth to the live weight of 100 kg, the length of the body (cm), the lifetime meat quality of the thickness of the scrape (mm), height of the longest back muscles (mm), the content of meat in the body (%). At the second stage of the research, the controlling fastenings of 200 goals of pigs and evaluation of feedst and meat qualities in the following indicators were carried out: the age of achieving a living mass of 100 kg (days), the average daily increase (d), feed consumption per 1 kg of growth (to. Units). Also determined: the length of the carcass (cm), the slaughter output (%), the thickness of the spick over 6-7 breast vertebrae (mm), the area of "muscular eye" (cm2), the mass of the rear third of the half carcass (kg), the flow of meat in the carcus (%). Then, genetic studies were compared with productivity indicators.Results. As a result of the research, it was found that in the tested animals, the frequency of the preferred allele I-PRKAG3 made 0.171-0.288, A-MC4R – 0.243-0.315, and C-MYOD1 – 0.064-0.477, respectively. The developed comprehensive system for assessing the breeding value of Yorkshire and Landrace pigs based on the marker genes PRKAG3, MC4R, and MYOD1 allowed us to identify the preferred alleles and determine correlation with productivity traits that provided increase in the average population values: for self-productivity - by 2-4%, for fattening traits - by 2.7-12.5%, for meat traits - by 2.8-34.4 %.Conclusion. The use of integrated system in pig breeding will speed up the selection work to increase the indicators of fattening and meat productivity of the created breeding herds of Yorkshire and Landrace pigs.


2021 ◽  
Vol 1 (2) ◽  
pp. 29-35
Author(s):  
N. I. Abramova ◽  
◽  
O. L. Khromova ◽  
M. O. Selimyan ◽  
◽  
...  

The use of foreign-bred bulls leads to changes in the genetic structure of the black-and-white breed population, which affects the level of population characteristics. The study was conducted to study the comparative variability and heritability of productive and reproductive characteristics of daughters of sires of domestic and foreign selection in pedigree livestock 10 250 cows 1st calving modern population of black-motley breed of the Vologda region. The superiority of the offspring of foreign bulls in the average indicators of all the studied traits was established with confidence (P<0,001). There were no significant differences in the level of variability of the characteristics of the descendants of domestic and foreign bulls. According to productive traits, the greatest variability of indicators was established for milk yield for 305 days of the 1st lactation Cv = 17,3 – 17,7%, therefore, selection on this trait will be more effective. The greatest share of the influence of bullsfathers, both domestic and foreign selection, in the total sum of all factors determining the value of traits in the population of the black-and-white breed, is determined by the milk yield of daughters η2 = 0,22 – 0,24, the age of the 1st fruitful insemination and the first calving η2 = 0,24 – 0,25. In further breeding work with the breed, it is necessary to obtain and use domestic producers that exceed the breeding value of foreign bulls.


Author(s):  
T. A. Krasnoshchekova ◽  
E. Yu. Zalyubovskaya

The determining factor in increasing the productivity of farm animals is the provision of comlete feeding of animals through feed additives. Among the functional feed additives containing essential trace elements, a special place under the conditions of the Amur region is given to microadditives of selenium, iodine and cobalt. The Amur region belongs to the biogeochemical region in which the defi ciency of I, Se and Co in feed is from 80 to 95 % compared to the average Russian indicators. The purpose of the work was to compare the eff ect of feed additives made from red alga Ahnfeltia, soy protein asparaginates separately and together with the drug “Creamino” on the growth, development and metabolism of young cattle. Under the conditions of the Amur region on the basis of their own experimental data in the rearing of young cattle, the eff ectiveness of the use of the drug “Creamino” and red alga Ahnfeltia has been scientifi cally justifi ed. The conducted scientifi c and economic and physiological experiments allowed us to determine the eff ect of red anfel on the growth and development, metabolic processes of replacement heifers and the growth rate of steers when they were fed soy protein asparaginates together with the drug “Creamino”. The evaluation of the economic effi ciency of feeding heifers I, Co and Se in organic form as part of soy protein asparaginates and red alga Ahnfeltia has shown a higher economic eff ect per 1 head per day in the 2nd experimental group, where the level of profi tability was 28,6 %. The results of the research and production experiment on steers have shown that from an economic point of view, it is advisable to use I, Co and Se in their rations in the form of soy protein asparaginates together with the drug “Creamino”, feeding of which can signifi cantly increase the economic eff ect, which is 1 head per day of 4,47 rubles with a profi tability level of 31,0 %.


2020 ◽  
Vol 89 (2) ◽  
pp. 141-150
Author(s):  
Zvonko Antunović ◽  
Željka Klir ◽  
Marcela Šperanda ◽  
Zdenko Lončarić ◽  
Boro Mioč ◽  
...  

The widespread selenium (Se) deficiency in soil and in feedstuffs from such soil, needs to be prevented by various methods. Recently, biofortification of Se has been carried out by soil fertilization and foliar application of crops. The aim of this study was evaluate the productive traits, Se status and blood metabolic profile in fattening lambs affected by Se biofortified corn. The research was conducted with 20 Merinolandschaf lambs of the average age of 70 days, during 30 days of fattening. The feed mixture of the control group contained corn (0.014 mg Se/kg dry matter, DM); in the experimental group (Se-BC), corn was biofortified (0.278 mg Se/kg DM). The feed mixture, hay and water were offered to lambs ad libitum. Blood was collected from the jugular vein on days 0 and 30 of experiment. Haematological indices were determined in whole blood, whereas concentrations of biochemical indices, enzyme activities, and concentrations of metabolic hormones were determined in serum. After the slaughter, samples of musculus semimembranosus, liver, kidney, lungs, spleen, peritoneum and heart tissues were taken. No significant differences were obtained in productive traits when feeding lambs with Se-BC. Higher concentrations of Se in lungs and liver (control: 0.26 and 0.75 mg/kg, respectively; Se-BC: 0.33 and 0.92 mg/kg, respectively) was determined in Se-BC compared to control. Higher activity of glutathione peroxidase enzyme in serum, higher erythrocyte count, higher content of haemoglobin and haematocrit in whole blood of Se-BC lambs were determined. The results of the study indicate the posibility of using Se-BC in lambs’ diets.


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