scholarly journals Integrated analysis of gene expression of solute carrier gene SLC19A3 with proven risk factors of microvascular complications in patients with type 2 diabetes

2021 ◽  
pp. 24-30
Author(s):  
K. Singh ◽  
T. Yu. Yuzvenko
Keyword(s):  
Gene Expression ◽  
Type 2 Diabetes ◽  
Target Gene ◽  
Diabetic Polyneuropathy ◽  
Nerve Fibers ◽  
Type 2 Dm ◽  
Duration Of Disease ◽  
Solute Carrier ◽  
Small Nerve

Diabetes mellitus (DM) is a chronic progressive disorder which leads to significant disability, morbidity and is likely to progress to become one of the most widespread conditions worldwide and as an additional burden to the healthcare system already reeling under the effects of the COVID-19 pandemic worldwide. Aim — to study the correlative variation in gene expression of SLC19A3 in type 2 diabetes patients with proven risk factors of diabetes complications. Materials and methods. In the study, 190 patients with type 2 DM were screened for diabetic peripheral neuropathy (DPN). DPN was confirmed in (n = 105) patients displaying symptoms of diabetic polyneuropathy with the involvement of small nerve fibers and large nerve fibers. Out of the total cohort, 45 patients with type 2 DM were shortlisted and randomized according to the severity of diabetic polyneuropathy, for assessment of the expression of the gene SLC19A3 in stage 1 of the gene expression study. Results. In the first stage of the study in patients with type 2 diabetes with diabetic polyneuropathy with the involvement of small nerve fibers and large nerve fibers we found that the difference in expression of the solute carrier gene SLC19A3 in patients with variable levels of neuropathy was not significant. As evident from the CT values and the value of ΔCT there was no statistically valid difference between the groups. The CT value of the target gene (SLC19A3) in all the three groups, in comparison to each other did not have significant difference in the initial phase of the investigation. In correlation to duration of disease the trend was similar, showing that duration of disease does not play an altering role in the expression of the target gene. In correlation to risk factor glycemic control (level of HbA1c) the expression of the target gene was slightly more profound in patients with an HbA1c of < 8.9 % as compared to the patients with HbA1c value > 9.0 %. Conclusions. Taking into consideration the results of the study it can be stated the expression of SLC19A3 is independent of the severity of diabetic polyneuropathy, duration of diabetes and the glycemic compensation in patients with type 2 diabetes.

scholarly journals Diagnosis and treatment of the early stages of diabetic polyneuropathy

2020 ◽  
pp. 56-65 ◽  
Author(s):  
V. N. Khramilin ◽  
A. N. Zavyalov ◽  
I. Yu. Demidova
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Nervous System ◽  
Early Diagnosis ◽  
Diabetic Polyneuropathy ◽  
Nerve Fibers ◽  
Skin Reactions ◽  
Small Nerve

Diabetic polyneuropathy (DPN) is the most common and earliest complication of diabetes mellitus and it may occur much earlier in patients with type-2 diabetes than in patients with type-1. Distal polyneuropathy can develop not only in diabetes mellitus, but also at the stage of prediabetes and even in patients with metabolic syndrome without impaired glycemic state. Hyperglycemia viewed as a major, but not the sole factor, responsible for development and progression DPN. The control of blood glucose as an obligatory step of therapy to delay or reverse DPN is no longer an arguable issue. Damage of peripheral nervous system in prediabetes and in the initial stages of diabetes mainly affects small nerve fibers, which also leads to disorders of the autonomic nervous system. Cardiac autonomic neuropathy diagnosed in 5-7.7% of patients at the time of diagnosis of type 1 and type 2 diabetes mellitus. For the early diagnosis of DPN, both routine assessment of peripheral sensation can be used, as well as specialized methods (sympathetic skin reactions, skin biopsy, confocal corneal microscopy, quantitative sensory tests) and validated questionnaires (Utah Early Neuropathy Scale) focused on assessing the function of small nerve fibers. Non-electrophysiological studies also were tested for early diagnosis of DPN: peripheral nerve sonoelastography, optical coherence tomography, MRI neurography, spiral positron emission CT (SPECT) with 123I-MIBG. DPN diagnosis in the pre-clinical stage very important, because treatment with diet and lifestyle intervention may be successful. The correlation between the severity of oxidative stress and the activity of antioxidant defense is considered as a potential mechanism for early nerves damage with hyperglycemia and as a possible target for therapeutic intervention. In this work, we will review prevalence, diagnostic approaches and potential treatment options for early diabetic polyneuropathy.

scholarly journals The Relationship Between Duration Disease and Glucose Blood Related to Subjective Compliance in Diabetes Mellitus

2017 ◽  
Vol 5 (2) ◽  
pp. 218 ◽  
Author(s):  
Nur Lailatul Lathifah
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Disease Duration ◽  
Subjective Complaint ◽  
Type 2 Dm ◽  
Duration Of Disease ◽  
Sugar Levels

Diabetes mellitus is often called the silent killer because it can affect all the organs of the body and cause several kinds of complaints. In 2013, Tambaksari Sub-district, Surabaya city has high DM case and become health problem because it has DM prevalence rate exceeding East Java prevalence 2,1% and bigger than DM prevalence rate in Indonesia that is 1.5%. The purpose of this study was to analyze the duration of disease and blood sugar levels with subjective complaints in patients with type 2 diabetes in Puskesmas Rangkah and Pacarkeling Surabaya. This research uses analytic observational with cross sectional method. The sample in this research that is patient of type 2 DM as much as 50 sample. The sampling technique was done by Simple Random Sampling, the researcher conducted the interview with the help of questionnaire. Data analysis using Chi Square test. Dependent variable in this research is subjective complaint, while the independent variable is disease duration and blood sugar level. Result of research indicate that there is correlation between duration of disease with subjective complaint in patient Type 2 DM (p = 0.049) and blood sugar level with subjective complaint In patients with type 2 diabetes (p = 0.004). Based on the analysis it can be concluded that there is a relationship between the duration of the disease and blood sugar levels with subjective complaints in patients with Type 2 diabetes. To the puskesmas in order to increase knowledge and information to patients with type 2 diabetes mellitus diabetes mellitus in the form of complications and how to handle it.Keywords:type 2 diabetes mellitus, disease duration, glucose blood, subjective complaint

Recreational Running Training Favourably Affects Antioxidants Gene Expression in Type 2 Diabetes Patients

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 52-LB
Author(s):  
MAYSA SOUSA ◽  
ARITANIA SANTOS ◽  
MARIA ELIZABETH R. SILVA
Keyword(s):  
Gene Expression ◽  
Type 2 Diabetes ◽  
Running Training ◽  
Diabetes Patients

Blockade of Renin Angiotensin System Ameliorates the Cardiac Arrhythmias and Sympathetic Neural Remodeling in Hearts of Type 2 DM Rat Model

2020 ◽  
Vol 20 (3) ◽  
pp. 464-478 ◽  
Author(s):  
Yomna M. Yehya ◽  
Abdelaziz M. Hussein ◽  
Khaled Ezam ◽  
Elsayed A. Eid ◽  
Eman M. Ibrahim ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Cardiac Arrhythmias ◽  
Sympathetic Nerve ◽  
Renin Angiotensin System ◽  
Diabetic Rats ◽  
Angiotensin System ◽  
Type 2 Dm ◽  
Type 2 Diabetic ◽  
Diabetes Induction

Objectives:: The present study was designed to investigate the effects of renin angiotensin system (RAS) blockade on cardiac arrhythmias and sympathetic nerve remodelling in heart tissues of type 2 diabetic rats. Methods:: Thirty-two male Sprague Dawley rats were randomly allocated into 4 equal groups; a) normal control group: normal rats, b) DM group; after type 2 diabetes induction, rats received 2ml oral saline daily for 4 weeks, c) DM+ ACEi: after type 2 diabetes induction, rats were treated with enalapril (10 mg/kg, orally for 4 weeks) and d) DM+ ARBs: after type 2 diabetes induction, rats were treated with losartan (30 mg/kg, orally for 4 weeks). Results:: In type 2 diabetic rats, the results demonstrated significant prolongation in Q-T interval and elevation of blood sugar, HOMA-IR index, TC, TGs, LDL, serum CK-MB, myocardial damage, myocardial MDA, myocardial norepinephrine and tyrosine hydroxylase (TH) density with significant reduction in serum HDL, serum insulin and myocardial GSH and CAT. On the other hand, blockade of RAS at the level of either ACE by enalapril or angiotensin (Ag) receptors by losartan resulted in significant improvement in ECG parameters (Q-T), cardiac enzymes (CK-MB), cardiac morphology, myocardial oxidative stress (low MDA, high CAT and GSH) and myocardial TH density. Conclusions:: RAS plays a role in the cardiac sympathetic nerve sprouting and cardiac arrhythmias induced by type 2 DM and its blockade might have a cardioprotective effect via attenuation of sympathetic nerve fibres remodelling, myocardial norepinephrine contents and oxidative stress.

scholarly journals Peripheral blood levels of CXCL10 are a useful marker for diabetic polyneuropathy in subjects with type 2 diabetes

2021 ◽  
Author(s):  
Pilar Ascaso ◽  
Ana Palanca ◽  
Sergio Martinez‐Hervás ◽  
María Jesús Sanz ◽  
Juan F. Ascaso ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Peripheral Blood ◽  
Diabetic Polyneuropathy ◽  
Blood Levels
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