scholarly journals Growth Response of Melati Plant Plant (Jasminum Sambac L.) Against Types of Media Plants and Types of Growing Agents

2020 ◽  
Vol 2 (2) ◽  
pp. 188-200
Author(s):  
Yuliatul Muslimah ◽  
Sumeinika Fitria Lizmah ◽  
Nur Fayanti
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Root Length ◽  
Alluvial Soil ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Media Types ◽  
The Difference ◽  
Jasminum Sambac ◽  
Number Of Shoots

Propagation of jasmine plants by cuttings often experience obstacles, especially to grow roots. Proper planting media and application of growth regulators (PGR) have an important role in the growth and development of plant cuttings roots. The purpose of this study was to determine the effect of planting media types and PGR on the growth of jasmine cuttings. This research was conducted in the experimental garden of the Faculty of Agriculture, Teuku Umar University, in March - May 2019. This study used a completely randomized design (CRD) with a factorial pattern of 3x3 with three replications. The treatment factors studied were (1) the type of planting media consisted of 3 levels namely alluvial soil, sand and husk charcoal, and (2) the type of growth regulator (ZPT) consisted of 3 levels namely control (without zpt), chemical (growtone) and organic (shallot extract). F test results on the analysis of variance showed that the planting medium had a very significant effect on the number of shoots 35 and 45 HST, number of leaves 15, 35 and 45 HST number of roots, root length, percentage of live cuttings. But the planting media had no significant effect on the number of shoots of 15 DAP. Growth regulator substances have a very significant effect on the number of shoots 35 HST, number of leaves 15, 35 and 45 HST, number of roots and root length. Significantly affect the number of shoots 45 HST and percentage of live cuttings, but no significant effect on the number of shoots 15 HST. This shows that the difference in growth of jasmine plant cuttings due to different planting media does not depend on growth regulators, and vice versa.

scholarly journals Pengaruh Konsentrasi Jus Lidah Buaya (Aloe cbinensis Baker) Terhadap Pertumbuhan Planlet Anggrek Hasil Silangan Dendrobium Morning Sun X Dendrobium Samarai

OrchidAgro ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Reo Sempana ◽  
Lia Amalia ◽  
Wahyono Widodo ◽  
Elly Roosma Ria ◽  
Noertjahyani Noertjahyani
Keyword(s):  
Tissue Culture ◽  
Experimental Design ◽  
Root Length ◽  
Aloe Vera ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
C 50 ◽  
Better Than ◽  
Number Of Shoots

This experiment aims was to study the effect of the concentration of aloe vera juice and to get the concentration of aloe vera juice which shows better growth of the number of leaves and the number of roots on the Dendrobium orchid plantlet in Hybrid-vitro results. This experiment was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Winaya Mukti University, which began in May to August 2019. The experimental design was used a Completely Randomized Design with 5 treatments in 5 replications and the treatments namely: A (0 g L-1 solution), B (25 g L-1 solution), C (50 g L-1 solution), D (75 g   L-1 solution), E (100 g L-1 solution). The results showed that consentration of B (25 g L-1 solution), C (50 g L-1 solution), D (75 g   L-1 solution) gave plantlet height, number of shoots, number of roots, and root length better than another treatments.

scholarly journals PENGARUH KONSENTRASI DAN LAMA PERENDAMAN STEK LADA (Piper nigrum) DALAM LARUTAN ZAT PENGATUR TUMBUH (AUKSIN)

2020 ◽  
Vol 20 (2) ◽  
pp. 158-169
Author(s):  
Teguh Supriyadi ◽  
Tyas Soemarah K.D ◽  
Endang Suprapti ◽  
Agus Budiyono
Keyword(s):  
Growth Regulators ◽  
Dry Weight ◽  
Piper Nigrum ◽  
Fresh Weight ◽  
Soaking Time ◽  
Root Dry Weight ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Shoots

The research entitled "The Effect of Concentration and Soaking Time of Pepper Cutters (Piper nigrum) in a Solution of Growth Regulatory Substances (auxin)" was carried out. Research was held on April – July 2020 in Green House of Agriculture Faculty, Tunas Pembangunan University, Jl. Balekambang Lor No. 1 Manahan, Surakarta. This study used a factorial experimental method based on a completely randomized design (CRD) consisting of 2 factors as treatment and 3 replications. The first factor, the concentration of growth regulators (auxin) (K), is divided into 3 levels, namely: 100 ppm (K1), 200 ppm (K2) and 300 ppm (K3); while the second factor, immersion time (P), also consists of 4 levels, namely: 2 hours (P1), 2.5 hours (P2), 3 hours (P3) and 3.5 hours (P4). The parameters observed in this study amounted to 9, with details as follows: Number of shoots, number of leaves, length of shoots, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight. Based on the results of research on pepper cuttings due to the concentration of growth regulators (auxin) and the duration of immersion, the results showed that: Soaking time had a significant effect on the observed parameters, namely plant length in P2 treatment (2.5 hours). And it did not affect the parameters of the number of shoots, number of leaves, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses).

2012 ◽  
Vol 28 (2) ◽  
pp. 82-90
Author(s):  
Rina Arimarsetiowati ◽  
Fitria Ardiyani
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Combined Treatment ◽  
Stem Diameter ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Half Strength ◽  
The Media ◽  
Number Of Leaves ◽  
High Level

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals PENGARUH JUMLAH MATA TUNAS BATANG ATAS DAN TINGGI BATANG BAWAH PADA SAMBUNG PUCUK TERHADAP PERSENTASE TUMBUH JAMBU AIR (Syzygium Samarangense)

2020 ◽  
Vol 7 (2) ◽  
pp. 99
Author(s):  
Supriyono Supriyono ◽  
Tarwa Mustopa ◽  
Nunuk Helilusiatiningsih ◽  
Fristama Maulana
Keyword(s):  
Significant Interaction ◽  
Treatment Combination ◽  
Design Factor ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Shoots

Water guava (Syzygium Samarangense) was a popular plant in Indonesia because it was fresh and sweet. The aim of this research was to analyze the effect of the number of shoots of the upper stems and height of the rootstock at the shoot graft on the percentage of growing water guava. The method used in this study was a completely randomized design, factor into one rootstock length, namely 10 cm, 15 cm, and 20 cm. and the second factor was the number of buds on the scion, namely 2, 3, and 4. The results showed that there was a significant interaction in the treatment combination of the number of 4 buds on the upper stem and the length of the rootstock 15 cm at 35 days after grafting affected the growth percentage and growth in the number of leaves and length of the scion.

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals PRODUCTIVITY Pueraria phaseoloides var. Javanica FERTILIZED WITH MANURE FROM CATTLE OFFERED CONCENTRATE CONTAINNING DIET SUPPLEMENTED WITH AMMONIUM SULFAT

Pastura ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 39
Author(s):  
Tjok. Istri Putri ◽  
Ni Putu Mariani
Keyword(s):  
Ammonium Sulfate ◽  
Weight Ratio ◽  
Dry Weight ◽  
Optimal Dosage ◽  
Pueraria Phaseoloides ◽  
Leaf Color ◽  
Randomized Design ◽  
Number Of Leaves ◽  
The Difference ◽  
Number Of Branches

This study aims to determine the optimal dosage of manure derived from cattle fed concentrated rations supplemented ammonium sulfate which can increase the productivity of Pueraria phaseoloidesvar .Javanica. The design used in this experiment was Completely Randomized Design (RAL) with 4 treatments and 5 replications. The four treatments were fertilized with doses of 10, 15, 20 and 25 tons / ha, respectively for the treatment of A, B, C and D, so overall there were 20 pot experiments. The study was conducted for 12 weeks. Observed variables were plant length, number of leaves, number of branches, dry weight (DW) of leaves, DW of stem DW, total BK, leaf color, leaf stem ratio (LSR) and leaf weight ratio”(LWR). The results showed that legumes treated A B, C and D produced high crop, leaf area, leaf color, LSR and LWR were not significantly different (P> 0.05). The treated legume C. resulted in a significantly higher leaf number than the treated legume A, B and D (P <0.05). The number of branches and DW leaves on legumes treated with C were significantly higher (P <0.05) than those receiving treatment A. and were not significantly different with legumes treated with B and D (P> 0.05). The DW of stem and total DW production from legume treated C were significantly higher with those treated with A and B (P <0.05) and the difference was not significant with those treated with D (P> 0.05). From the results of the study it can be concluded that legume treated with C or fertilized with manure derived from cattle fed concentrate containning diet supplemented ammonium sulfate at a dose of 20 tons / ha yield of leguminous productivity Peuraria phaseoloides var. javanica maximum. Keywords: manure, pueraria phaseoloides, productivity.

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

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