scholarly journals In-Vitro androgenesis in papaya (Carica papaya L.) cv. Pusa Nanha

2015 ◽  
Vol 7 (1) ◽  
pp. 273-278 ◽  
Author(s):  
Gyanchand Gyanchand ◽  
Manoj Kumar Sharma ◽  
Sandeep Kumar ◽  
Sushma Sagar ◽  
Vinay Kumar ◽  
...  
Keyword(s):  
Anther Culture ◽  
Carica Papaya ◽  
Industrial Applications ◽  
Liquid Form ◽  
Induction Rate ◽  
In Vitro Androgenesis ◽  
Embryo Induction ◽  
Micro Propagation ◽  
The Media

Papaya (Carica papaya L.) is an economically important fruit crop of tropics and subtropics. It has high nutritional value, as well as medicinal and industrial applications. Papaya is a polygamous species with three sex types male, female, and hermaphrodite. Conventional methods of papaya breeding are time consuming and needs advent of anther culture which may be effective for shortening of breeding cycles. The present study on in vitro androgenesis in papaya cv. Pusa Nanha observed the highest embryo induction rate (8.0%) when anthers werecultured on agar medium with 0.1 mg/L BA and 0.1 mg/L NAA after incubation in liquid MS medium with 2.0% sucrose for 7 days at 35ºC. The high temperature (35ºC) was more suitable for embryo induction in papaya than slightly low temperature (25ºC). At these both temperatures longer incubation of anthers in water reduced embryo induction rate. Sugar starvation results were ambiguous. Shoots were also developed in the media when used in liquid form. The highest rooting (75.0%) was observed at 2.0 mg/L IBA. Increasing IBA concentration reduced rooting. All well rooted plants were hardened in hardening chamber and successfully transferred to field. The present findings indicated that anther culture can be efficiently contributed for the direct micro-propagation of papaya plants. This study would also be helpful to the researchers to develop more efficient anther culture protocols for further improvement of papaya through in vitro androgenesis.

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

scholarly journals Optimization of micropropagation by different concentration of vitamins and sucrose in St. John’s Wort (Hypericum perforatum)

2015 ◽  
Vol 71 (1) ◽  
pp. 67-79
Author(s):  
Sahar Khakpour ◽  
Alireza Motallebi-Azar ◽  
Bahman Hosseini ◽  
Saeede Alizadeh-Salte ◽  
Abbas Hasani
Keyword(s):  
Nicotinic Acid ◽  
Hypericum Perforatum ◽  
Shoot Proliferation ◽  
Leaf Length ◽  
Morphological Attributes ◽  
Acid Control ◽  
Micro Propagation ◽  
The Media ◽  
Negative Side Effects

Abstract In order to approach optimal micropropagation of Hypericum perforatum, it will be necessary to optimize shoot proliferation stage in in vitro culture. This study was conducted to investigate the effects of different concentrations of B group vitamins; Thiamine HCl, Pyridoxine HCl, Nicotinic acid (control and 100 fold of MS) and Sucrose (30 and 40 g.l−1) on shoot proliferation. For this purpose, Stems with one node were taken from in vitro shoots and cultured on MS medium. All cultures kept at 16h light/8h photoperiod and 25 ±2 °C in growth chamber. Results showed that the highest number of shoots and leaves were achieved when explants cultured in media containing 40 g.l−1 sucrose with 100 fold of MS vitamins. The highest shoots and leaf length were obtained with medium supplemented with 30 g.l−1 sucrose. Nicotinic acid concentrations had an important role in length of the leaves. The highest number of nodes achieved in the media containing 40 g.l−1 sucrose with both concentrations of Nicotinic acid. After two month growing plantlets, light (LGN) and dark glands number (DGN) were counted. Maximum number of LGN was observed in the media containing 30 g.l−1 sucrose with 100 fold of Thiamine and Pyridoxine. However, The Highest number of DGN achieved in the media containing 40 g.l−1 sucrose with 100 fold of Thiamine or Pyridoxine. Increasing of sucrose and vitamins concentrations were efficiently improved in vitro proliferation and some morphological attributes without negative side effects. Therefore, use of high levels of sucrose and vitamins were useful on micro-propagation of Hypericum perforatum.

scholarly journals In vitro androgenesis in triticale

2017 ◽  
Vol 1 (1) ◽  
pp. 99-100
Author(s):  
Oksana Ivanovna Zaitseva
Keyword(s):  
Genetic Variation ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
External Factors ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines ◽  
In Vitro Androgenesis

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.

scholarly journals Efecto de los factores determinantes para el establecimiento de microesquejes de papaya (Carica papaya L.)

Respuestas ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 5-12
Author(s):  
Jesús Arturo Ramírez Sulvarán
Keyword(s):  
Production Process ◽  
Key Words ◽  
Statistical Method ◽  
Carica Papaya ◽  
The Other ◽  
Tropical Fruits ◽  
Important Goal ◽  
Experimental Procedure ◽  
Micro Propagation

El objetivo fue determinar los efectos de los factores para el establecimiento de microesquejes de papaya. Se incluyeron los efectos de los factores: 1. Protocolo de desinfección. 2. Variedad. 3. Edad de la planta donadora. 4. Número de yemas por microesquejes. 5. Los componentes hormonales en los medios de cultivo y 6. La temperatura de desarrollo de los microesquejes. Los resultados se analizaron estadísticamente mediante tablas de contingencia. También se encontró la correlación entre las variables. Los resultados indican que las mejores condiciones para el establecimiento de los microesquejes fueron: 1.Desinfección con hipoclorito al 1,5% durante 5 minutos. 2. La variedad de papaya Roja Melonera. 3. La edad optima de las plantas donadora fue a los 60 días. 4. Los mejor microesquejes eran los que poseía 2 yemas. 5. La relación hormonal optima fue ANA 0,1 mg/l y BAP 0,5 mg/l. 6. La temperatura de desarrollo óptima fue a 27° C. Se concluyó que todos los factores estudiados presentaron efecto sobre el desarrollo y crecimiento de los microesquejes de papaya. De esta forma es necesario abordar estos procesos con un enfoque multifactorial.Palabras clave: Carica papaya, microesqueje, establecimiento, multifactorial. AbstractPrevious studies on apical and axilar microesqueje micropropagation of papaya have been lined up and without a systemic working. Consequently, it arises the actual research, which brough into study all the variables and parameters and their relationships, in order to improve the vitroplants production process, more efficiently. The main purpose of this study was to establish the effects of the former and in vitro factors, which have incidence upon the establishment of microesquejes. The effects of the former variables previous to the in vitro growing, which were studied, were: Sanitizing procedure, variety incidence, mother plant age incidence, among other ones. On the other hand, the in vitro variables studied were: temperature and hormonal compositions of the crops. Respuestas Año 15 No. 1 Junio 2010 ISSN 0122-820X This research was held at the vegetal biotechnology laboratory, which belongs to the biotechnology complex of the Universidad Francisco de Paula Santander in “Los Patios” Norte de Santander, Colombia. The experimental procedure was held with two months old plants from “Maradol” and “Roja Melonera” varieties. In order to accomplish the different effects of each one of the variables, it was used qualitative statistical method for their measurements. The final results showed that all factors were important to the papaya microesquejes micro propagation. As a result, the actual conclusion was to introduce the necessity to give a systemic focusing to the process in order to improve the efficiency in the vitroplants production, where all the factors and their relationships must be considered. Finally, the most important goal of this study is that all these results could be extended beyond other researches related to tropical fruits micro propagation, so important to the tropical micro propagation workers. Key Words: Carica papaya, microcuttings, establishment, multifactors.

scholarly journals A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

2020 ◽  
Vol 36 ◽  
pp. 66-74
Author(s):  
Mohammad Javad Mahmoudi Meimand ◽  
Mohammad Hossein Shamshiri ◽  
Khalil Malekzadeh ◽  
Mohammad Reza Dehghani
Keyword(s):  
Sucrose Concentration ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
The Media ◽  
Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efficiency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

scholarly journals ​Effect of Different Media on In vitro Rooting in Different Cultivar of Lilium longiflorum Cv. Elite, Brunello, Cordelia

2021 ◽  
Author(s):  
Manoj Kundu ◽  
Suresh Kumar ◽  
Rajesh Lathar ◽  
Sakshi .
Keyword(s):  
Tissue Culture ◽  
Cost Effective ◽  
Lilium Longiflorum ◽  
Global Market ◽  
Root Regeneration ◽  
Planting Material ◽  
Micro Propagation ◽  
The Media ◽  
Increasing Demand

Background: Lilium (Lilium longiflorum Thunb.) belongs to the family Liliaceae and is a native of Northern Hemisphere (up to South Canada and Siberia). Conventionally Lilium can easily be propagated by sexual and asexual methods of propagation but these prevalent methods are not capable of meeting the increasing demand in domestic and global market. Generally, Lilium is propagated through bulbs but, limited number of bulbs per plant, long dormancy period of bulbs which again results into non-availability of planting material throughout the year. Keeping in view the above facts, the present study was undertaken with the following objective: “To standardize the cost effective protocol for micro propagation of lilium to produce disease free and true to type plants at a faster rate”. Methods: The present investigation was carried out in the Tissue Culture Laboratory of the Centre for Research and Application in Plant Tissue Culture. The experiment was laid out in a C.R.D. (Factorial) with three replications. In vitro raised bulblets were separated out and were transferred on to the root regeneration media. Different levels of NAA were used in MS media for the rooting of in vitro raised bulblets and percent rooting of plantlet is recorded. Result: It was interesting to note that the media LR-3 (MS + NAA 1.0 mg/l) is most efficient for rooting in all type of cultivars. All the three cultivars used responded very poor on media LR-1 (MS basal).

scholarly journals Micro Propagation of Several Potted Anthurium Accessions Using Spathe Explants

2012 ◽  
Vol 11 (1) ◽  
pp. 59 ◽  
Author(s):  
Kurniawan Budiarto
Keyword(s):  
In Vitro Propagation ◽  
Light Conditions ◽  
Defined Media ◽  
Micro Propagation ◽  
New Varieties ◽  
The Media ◽  
Ornamental Crops ◽  
Propagation Technique ◽  
Genotypic Alteration

The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.

scholarly journals Optimization of in vitro asymbiotic seed germination protocol for Serapias vomeracea

2019 ◽  
Vol 3 (3) ◽  
pp. 143-151 ◽  
Author(s):  
Arda Acemi ◽  
Fazıl Özen
Keyword(s):  
Seed Germination ◽  
Liquid Form ◽  
Tetrazolium Chloride ◽  
Asymbiotic Seed Germination ◽  
Surface Sterilization ◽  
Seed Surface ◽  
Sterilization Method ◽  
Germination Success ◽  
The Media

Abstract Serapias vomeracea is an economically important orchid species which is over-collected from nature, because of its glucomannan-rich tubers. Thus, optimization of in vitro culture methodology in this species is required to meet industrial needs and to secure its populations in nature. This study aimed to optimize the surface sterilization protocol for S. vomeracea seeds and to select the optimal seed germination medium by comparing the commonly used media in in vitro orchid culture. During seed surface sterilization, ethyl alcohol (EtOH) pre-treatment prior to sodium hypochlorite (NaOCl) treatment increased the disinfection success and viable seed yield when examined using the triphenyl tetrazolium chloride (TTC) seed viability test. Also, low-g force centrifugation as an additional step in the surface sterilization method separated the seeds without embryo from the viable seeds and thereby decreased potential counting errors after incubation. Comparison of media showed that solid Knudson C (KN) medium induced the highest number of germinated seeds. However, seed germination success of Lindemann (LN) and Vacin & Went (VW) media was found to be higher when the media was used in liquid form. Half-strength liquid VW was the only medium that induced higher germination success than the other full-strength media. The highest number of ungerminated seeds was found when using KN medium whereas liquid VW medium gave the lowest number. In general, protocorm formation was triggered when the media were used in liquid form. However, rhizoid elongation was suppressed in liquid media. These findings suggest that this optimized seed surface sterilization method offers a simple and effective alternative to classical methods. Additionally, solid KN medium may be considered as a cost-effective and reliable alternative to other commonly-used complex media in S. vomeracea cultures.

scholarly journals Response of some Tunisian bread wheat varieties (Triticum aestivum L.) to anther culture system

2018 ◽  
Vol 19 (1) ◽  
pp. 1-9
Author(s):  
Ali Ltifi ◽  
◽  
nissa Sahli ◽  
Wejden Brahmi ◽  
Insaf Nefzi ◽  
...  
Keyword(s):  
Anther Culture ◽  
Bread Wheat ◽  
Culture System ◽  
Culture Media ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Wheat Varieties ◽  
In Vitro Androgenesis ◽  
Good Number

This study focusedon wheat haploid production by anther culture. The main purpose wasto compare the in vitro androgenesis response often wheat Tunisian bread wheat varieties on two different induction media. The anthers were introduced in vitro when microspores were in the middle uninucleate stage and obtainedfrom treated spikes at 4° C for two weeks. The culture media 190-2 and BAC3 were used. Plants regenerated from induced embryos and calli were grown on the 190-2 medium without any growth regulators. The response to anther culture was different between the studied varieties. Androgenesis induction was better on the 190-2 medium than on BAC3 medium. In addition, the development of calli and the regeneration of embryos were better on 190-2 medium than on BAC3. Both green plantlets and albina were regenerated from induced embryos on the 190-2 medium, while no green plant regenerated from the embryos growingon BAC3 medium. Four varieties over ten studied gave good number of green plants. These results indicatedthat 190-2 medium was most favorable for the in vitro androgenesis of the Tunisian bread wheat varieties.

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