scholarly journals Reaction of grapevine rootstocks and cultivars to Meloidogyne incognita, M. arenaria and M. hapla

Agriscientia ◽  
2021 ◽  
Vol 38 (1) ◽  
pp. 93-98
Author(s):  
Ricardo Andreé Vega-Callo ◽  
Juan José Tamo-Zegarra ◽  
Cristiano Belle
Keyword(s):  
Meloidogyne Incognita ◽  
Experimental Unit ◽  
Root Knot Nematode ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Salt Creek ◽  
Reproduction Factor ◽  
Meloidogyne Species

This study aimed to evaluate the reaction of six grapevine rootstocks (MGT 101-14, Ritcher 110, Paulsen 1103, K 5BB, SO4, Salt creek) and two cultivars (Quebranta and Torontel) to three species of the root-knot nematode (Meloidogyne incognita, M. arenaria and M.hapla). The experiment was performed as a completely randomized design with an 8 × 3 factorial scheme and six replicates per treatment. The experimental unit in each replicate comprised a grapevine cutting planted in 3 kg bags with sterilized soil. Cuttings were inoculated with 5000 eggs + juveniles (J2) of M. incognita, M. arenaria and M. hapla. Six months after inoculation, plants were removed from the bags, and the reaction was determined by evaluating the number of galls (NG), number of nematodes per gram of root (NNGR), and reproduction factor (RF). The evaluated rootstocks, MGT 101-14, Ritcher 110, Paulsen 1103, K 5BB, SO4 and Salt Creek, were resistant to M. incognita, M. arenaria and M. hapla, except for Salt creek, which was susceptible to the latter. The Quebranta and Torontel cultivars were susceptible to the  Meloidogyne species under study.

scholarly journals Resistance sources to root-knot nematode Meloidogyne enterolobii in Solanum species

2020 ◽  
pp. 303
Author(s):  
Jadir Borges Pinheiro ◽  
Giovani Olegario da Silva ◽  
Jhenef Gomes de Jesus ◽  
Danielle Biscaia ◽  
Raphael Augusto de Castro e Melo
Keyword(s):  
Solanum Species ◽  
Experimental Unit ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Second Stage ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Plant Grown ◽  
Reproduction Factor

The objective of this work was to prospect sources of resistance to root-knot nematode Meloidogyne enterolobii in Solanum species with potential to be used as rootstocks for cultivated Solanaceae. Nine accessions of Solanum sessiliflorum, 27 accessions of S. lycocarpum, 21 accessions of S. acanthodes, 22 accessions of S. scinericum and 26 accessions of S. scuticum for resistance to M. enterolobii. Rutgers and Nemadoro tomatoes were used as susceptible and resistant controls, respectively. The experiment was conducted in a greenhouse at Embrapa Vegetables, Brasília-DF, Brazil, in a completely randomized design with six replications. The experimental unit was a represented by a single plant grown in a plastic pot containing 3 L of substrate. 4000 eggs and eventual juveniles of second stage M. enterolobii were inoculated per pot. At 119 days after inoculation, gall index (Gi), egg mass index (EMI), number of eggs per root gram (NE) and reproduction factor (Fr) were evaluated. Data were subjected to analysis of variance and grouping of treatments by Scott-Knott. It was verified that S. acanthodes and S. Lycocarpum are species with high resistance to M. enterolobii, with accessions being classified identified as immune. S. scuticum also has great potential, as several resistant accessions were identified, although some accessions were quite susceptible; whereas for S. subinerme only 4 resistant accessions were identified, although all others presented a reproduction factor much lower than tomato cv. Nemadoro as control; and all evaluated S. sessiliflorum accessions were susceptible.

scholarly journals Reaction of melon genotypes to Meloidogyne incognita and Meloidogyne javanica

2016 ◽  
Vol 46 (1) ◽  
pp. 111-115
Author(s):  
Guilherme Matos Martins Diniz ◽  
Willame dos Santos Candido ◽  
Renato Silva Soares ◽  
Lucas da Silva Santos ◽  
Marcus Vinícius Marín ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Santa Cruz ◽  
Breeding Programs ◽  
Northeast Region ◽  
Resistant Cultivars ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT Melon is one of the most economically important crops cultivated in Brazil, especially in the Northeast region. However, as its cultivation increases, phytosanitary problems arise, including those caused by nematodes, which are most effectively controlled using resistant cultivars. This study aimed at assessing the reaction of muskmelon genotypes, in terms of resistance to Meloidogyne incognita and M. javanica infestation. The experiment was conducted under greenhouse conditions using a completely randomized design, in a 2 x 15 factorial scheme, with six replications. A total of 15 muskmelon genotypes were evaluated and the 'Santa Cruz Kada' tomato was used as a susceptible control. The total number of eggs and juvenile nematodes in the roots and the reproduction factor were used to assess the genotype reaction. None of the genotypes was resistant to M. incognita. Eight genotypes were classified as resistant to M. javanica and promising for use in melon breeding programs.

scholarly journals Influence of Meloidogyne incognita race 1 on the development of clones of Coffea canephora, variety “Jequitibá Incaper 8122”

2020 ◽  
Vol 87 ◽  
Author(s):  
Lilian Katiany Castello Rabello Zinger ◽  
Fernando Domingo Zinger ◽  
Fábio Ramos Alves ◽  
Waldir Cintra de Jesus Junior ◽  
Angelo Oliveira Gonçalves ◽  
...  
Keyword(s):  
Coffea Canephora ◽  
Root Knot Nematode ◽  
Fresh Matter ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Race 1 ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT: Root-knot nematode is one of the most important phytosanitary problems for Conilon coffee, as it reduces productivity and is difficult to handle. We aimed at studying the infectivity and damage caused by M. incognita race 1 in the “Jequitibá Incaper 8122” intermediate maturity coffee variety. The experiment was conducted in a greenhouse, in completely randomized design, with five replicates. The clones composing the variety “Jequitibá Incaper 8122” were inoculated with 2,000 eggs + second-stage juveniles of M. incognita race 1. Uninoculated plants were the control. Evaluations were performed 180 days after inoculation, considering the plant height (H), stem diameter (SD), number of leaves (NOL), leaf area (LA), number of plagiotropic branches (NPB), number of nodes (NN), chlorophyll content (CHLO), shoot dry matter (SDM), root fresh matter (RFM), final population (FNP), and reproduction factor (NRF). The nematode reduced NOL in clones 208 and 209, NRF in clones 201, 203, 207 and 208, NN in clones 203, 207, 208 and 209, CHLO in clones 201, 204, 206, 207 and 209, SDM in clones 201, 203, 204 and 205 and RFM in clones 205 and 207. M. incognita race 1 FNP and NRF were larger in clones 208, 201, 207 and 203. Clone 202 had FNP and NRF equal to zero, being immune to the nematode. Clone 206 presented the lowest NRF value among clones parasitized by M. incognita.

scholarly journals Effect of drying and/or warming piglets at birth under warm farrowing room temperatures on piglet rectal temperature over the first 24 h after birth

2021 ◽  
Author(s):  
Katherine D Vande Pol ◽  
Andres F Tolosa ◽  
Caleb M Shull ◽  
Catherine B Brown ◽  
Stephan A S Alencar ◽  
...  
Keyword(s):  
Birth Weight ◽  
Birth Control ◽  
Rectal Temperature ◽  
Fixed Effects ◽  
Random Effect ◽  
Repeated Measure ◽  
Experimental Unit ◽  
Concentration Data ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract Piglets experience a decline in body temperature immediately after birth, and both drying and warming piglets at birth reduces this. However, these interventions may have less effective at higher farrowing room temperatures. This study was carried out at a commercial facility to compare the effect of drying and/or warming piglets at birth on postnatal rectal temperature (RT) under relatively warm farrowing room temperatures (26.6 ± 2.09°C). Forty-five sows/litters were used in a completely randomized design to compare three Intervention Treatments (applied at birth): Control (no treatment); Warming (piglets placed in a plastic box under a heat lamp for 30 min); Drying+Warming (piglets dried with desiccant and warmed as above). Temperatures in the warming boxes over the study period averaged 37.7 ± 2.75°C. At birth, piglets were weighed; RT temperature was measured at 0, 10, 20, 30, 45, 60, 120, and 1440 min after birth. Blood samples were collected at 24 h after birth from a subsample of one piglet from each birth weight quartile within each litter to measure plasma immunocrit concentration. Data were analyzed using PROC MIXED of SAS with litter as the experimental unit; and piglet a subsample of litter. The model for analysis of piglet rectal temperature included fixed effects of treatment, measurement time (repeated measure), the interaction, and the random effect of sow. Compared to the Control, piglet RT were higher (P ≤ 0.05) for the Warming treatment between 10 and 60 min, and higher (P ≤ 0.05) for the Drying+Warming treatment between 10 and 120 min after birth. Rectal temperatures were higher (P ≤ 0.05) for the Drying+Warming than the Warming treatment between 20 and 120 min. Responses to drying and/or warming were greater for low birth weight piglets (< 1.0 kg) than heavier littermates, but were generally less than observed in previous experiments with similar treatments carried out under cooler temperatures. Piglet immunocrit values were lower (P ≤ 0.05) for the Drying+Warming treatment compared to the other treatments, which were similar (P > 0.05). Immunocrit values tended (P = 0.10) to be lower for light (< 1.0 kg) compared to heavier birth weight piglets. In conclusion, drying and warming piglets at birth was more effective for reducing piglet RT decline after birth than warming alone, though the effect was less than observed in previous studies carried out under cooler farrowing room temperatures.

scholarly journals Digestibility and bioavailability of phosphorus from spray-dried yeasts in the diet of starting pigs (15-30 kg)

2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  
Keyword(s):  
Live Weight ◽  
Basal Diet ◽  
Relative Bioavailability ◽  
Mean Value ◽  
Experimental Unit ◽  
Available Phosphorus ◽  
Standard Curve ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Spray Dried

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Broccoli yield in response to top-dressing fertilization with green manure and biofertilizer

Revista CERES ◽  
2011 ◽  
Vol 58 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Gilberto Bernardo de Freitas ◽  
Márcio Sousa Rocha ◽  
Ricardo Henrique Silva Santos ◽  
Letícia Monteiro da Silva Freitas ◽  
Leandro de Almeida Resende
Keyword(s):  
Green Manure ◽  
Mineral Fertilizer ◽  
Dry Mass ◽  
Experimental Unit ◽  
Productive Performance ◽  
Mineral Fertilization ◽  
Randomized Design ◽  
Protected Cultivation ◽  
Completely Randomized Design ◽  
Organic Compost

The objective of this work was to evaluate the productive performance of broccoli under different top-dressing organic fertilizations. The experiment was conducted under protected cultivation, in a completely randomized design with four replications, with two plants per experimental unit. Broccoli seedlings were produced in a commercial substrate in styrofoam trays. The seedlings were transplanted to plastic pots containing 10.0 L of substrate made up of subsoil and organic compost at the ratio of 3:1 (v/v), respectively, which is equivalent to about 20.0 t ha-1 of organic compost at planting. After seedling establishment, the top-dressing fertilization treatments were applied: gliricidia biomass associated or not with liquid biofertilizer of cattle manure to the soil and bokashi. Two control treatments were established: one with mineral fertilization recommended for the crop and the other without top-dressing fertilization. The broccoli production was evaluated (commercial standard). Plants that received mineral fertilizer were more productive, however, they were not significantly different (p>0.05), by Dunnet test, from the plants fertilized with 2.5 t ha-1 gliricidiabiomass (dry mass) associated with liquid biofertilizer (2.0 L m-2) applied to soil. Top-dressing fertilizations with only gliricidia, at 2.5 and 5.0 t ha-1 of biomass (dry mass), resulted in no significant increase in production of broccoli inflorescence. The use of bokashi in addition to gliricidia biomass and liquid biofertilizer reduced the efficiency of the fertilization compared with plants that received only gliricidia and liquid biofertilizer.

scholarly journals Semi purified glycerins in growing and finishing pigs feeding (30-90 kg)

2014 ◽  
Vol 15 (1) ◽  
pp. 221-236 ◽  
Author(s):  
Liliane Maria Piano Gonçalves ◽  
Ivan Moreira ◽  
Paulo Cesar Pozza ◽  
Paulo Levi de Oliveira Carvalho ◽  
Juliana Beatriz Toledo ◽  
...  
Keyword(s):  
Vegetable Oil ◽  
Control Diet ◽  
Carcass Traits ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Experimental Unit ◽  
Finishing Pigs ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Performance Results

Two experiments were carried out to determine the nutritional value and to evaluate the performance and carcass traits in the growing-finishing pigs fed on two types of semipurified glycerin, which were made from vegetable oil (SPGV) and mixed of animal + vegetable oil (SPGM). In the digestibility trial (experiment I) 32 crossbreed barrows were used with initial body weight of 45.08 ± 4.11kg. It were used three levels of inclusion (6, 12 e 18%) of semipurified glycerin (SPGV and SPGM) in the basal diet. The metabolizable energy (ME) value of glycerin were estimated by regression of ME (kcal/kg) intake associated with glycerin vs. glycerin intake (kg). The values of ME (kcal/kg) obtained were: 2,731 for SPGV and 2,210 for SPGM. In experiment II, 72 pigs (30.08 ± 1.65 to 60.58 ± 3.57) in growing and (60.83 ± 2.63 to 93.79 ± 5.72) in finishing phase, were allotted in a completely randomized design in a 2 x 4 + 1 factorial scheme, with two types of semipurified glycerin (SPGV and SPGA), four inclusion levels (4; 8; 12 and 16%), eight experimental unit and a control diet containing no glycerin (0%). All pigs were slaughtered to evaluate the carcass traits. The performance results suggest that it is feasible to use up to 16% of both semipurified glycerins on growing and finishing pigs feeding, without impairing performance and carcass traits.

scholarly journals PENAMBAHAN BEBERAPA JENIS BAHAN NUTRISI PADA MEDIA PERBANYAKAN UNTUK MENINGKATKAN VIRULENSI BEAUVERIA BASSIANA TERHADAP HAMA WALANG SANGIT

2012 ◽  
Vol 12 (1) ◽  
pp. 64-70
Author(s):  
Ni Siluh Putu Nuryanti ◽  
Lestari Wibowo ◽  
Abdul Azis
Keyword(s):  
Beauveria Bassiana ◽  
Experimental Unit ◽  
Randomized Design ◽  
Rice Bug ◽  
Completely Randomized Design ◽  
Chitosan Powder

 A research was conducted to increase the virulence of Beauvaria bassiana to the rice bug, L.  acuta, by adding four different substances (materials) to the rice media to grow the fungus. The research was conducted from April to August 2011 at Polinela Laboratory, Bandar Lampung.  To do this a set of experimental unit consisting of five treatments and five replications were arranged in completely randomized design. The  four treatments were media made up of rice (rice based media) each given or added with four different materials such as rice skin powder, corn sugar, grasshopper powder and chitosan powder. The result showed that B. bassiana grown on rice media added with grasshopper powder or rice skin powder were able of causing mortality to the L. acuta as high as 78% and 71%, respectively, meaning that both mortalities were much higher than that of other replication. The virulence values of Beauveria bassiana  grown on those both powder were also higher than that of three others.

scholarly journals Do the stage of plant and initial population of root knot nematode influence on sweet pepper development?

O Biológico ◽  
2020 ◽  
Vol 82 (1) ◽  
pp. 1-10
Author(s):  
Samara A. de Oliveira ◽  
Juliana M. O Rosa ◽  
Juliana Eulálio ◽  
Claudio Marcelo G. de Oliveira
Keyword(s):  
Sweet Pepper ◽  
Initial Population ◽  
Root Knot Nematode ◽  
One Pot ◽  
Leaf Pair ◽  
Final Population ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Shoot Weight ◽  
Reproduction Factor

The aim of this study was to investigate the response of three different stages (one, three and five leaf pairs) of sweet pepper (Capsicum annuum cv. Orazio) seedlings to five inoculation levels of Meloidogyne enterolobii (zero (control), 300, 1000, 3000 and 10000) under greenhouse conditions. Each plant was cultivated in one pot filled with 3.8 L of substrate. The test was a completely randomized design with four replications. The plants were assessed 60 days after inoculation, plant shoot weight, final population of nematodes, and reproduction factor were measured. The results were fitted to Seinhorst model: Y = m + (1-m). ZPi –T. The results showed a tolerance limit (T) of 2,500 nematodes for plants with one and three leaf pair, and 8,500 nematodes for the five-leaf pair plant.

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