scholarly journals KARAKTERISTIK FENOTIP DAN GENOTIP MIKROSIMBION SPONS NIPHATES SP SEBAGAI BIOMATERIAL POTENSIAL PENDEGRADASI POLI AROMATIK HIDROKARBON

2018 ◽  
Author(s):  
Ismail Marzuki ◽  
sinardi ◽  
Asmeati

Sponges are often used as a reference for pollutant bioindicators, (pollution of heavy metals and poly aromatic hydrocarbons. Porosity of the sponge body is used as host microorganisms, especially bacteria and fungi. Phenotypic characterization and symbiotic genotypes of Niphates sp. molecular rRNA gene 2 of Nephates sp as symbionts isolates showed a positive reaction to methyl red (acid producer), VR, citrate (has a genetic carrier enzyme), lactose and catalase (acidic). This data shows that both isolates of Niphates sp can make carbon as a nutrient source of energy: Genotypic analysis of 2 isolates of Nephates sp in the phases of amplification and alignment of the sample gene homolog series on the Bank gene concluded that the 2 isolates were Bacillus cohnii strain DSM 6307 (BC) and Bacillus pumilus strain GLB197 (BP). (Fenantrena) shows growth cell cells based on parameters of increased optical density, gas bubbles formed, changes in media pH and fermentation odor arises.

2018 ◽  
Vol 3 (2) ◽  

In the recent years attempt to find cost-effective and ecological ways to deal with noxious waste led mankind to focus on the use of microbes for the degradation of pollutants. These environmental friendly remediation methods employs the microbial naturally occurring catabolic capabilities to alter, vitiate or accrue a large number of pollutants including poly aromatic hydrocarbons, polychlorinated biphenyls, radio nuclides ,heavy metals etc. High-through put analyses of environmentally relevant microbes provides an insight of their major degradative pathways as well as their competence to acclimate to altering environmental conditions.


Author(s):  
Ashish Khandelwal ◽  
Lata Nain ◽  
Shashi Bala Singh ◽  
Eldho Varghese ◽  
Anamika Sharma ◽  
...  

2010 ◽  
Vol 60 (3) ◽  
pp. 603-609 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).


2018 ◽  
Vol 52 (21) ◽  
pp. 12740-12747 ◽  
Author(s):  
Yang Liu ◽  
Qunying Dai ◽  
Xiuqi Jin ◽  
Xudong Dong ◽  
Juan Peng ◽  
...  

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 754-759 ◽  
Author(s):  
Paulina Corral ◽  
Angela Corcelli ◽  
Antonio Ventosa

An extremely haloalkaphilic archaeon, strain T26T, belonging to the genus Halostagnicola , was isolated from sediment of the soda lake Bange in the region of Tibet, China. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain T26T was closely related to Halostagnicola alkaliphila 167-74T (98.4 %), Halostagnicola larsenii XH-48T (97.5 %) and Halostagnicola kamekurae 194-10T (96.8 %). Strain T26T grew optimally in media containing 25 % (w/v) salts, at pH 9.0 and 37 °C in aerobic conditions. Mg2+ was not required for growth. The cells were motile, pleomorphic and Gram-stain-variable. Colonies of this strain were pink pigmented. Hypotonic treatment caused cell lysis. The polar lipids of the isolate consisted of C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and minor phospholipids components. Glycolipids were not detected, in contrast to the two neutrophilic species of this genus. The genomic DNA G+C content of strain T26T was 60.1 mol% and DNA–DNA hybridization showed a relatedness of 19 and 17 % with Halostagnicola alkaliphila CECT 7631T and Halostagnicola larsenii CECT 7116T, respectively. The comparison of 16S rRNA gene sequences, detailed phenotypic characterization, polar lipid profile and DNA–DNA hybridization studies revealed that strain T26T belongs to the genus Halostagnicola , and represents a novel species for which the name Halostagnicola bangensis sp. nov. is proposed. The type strain is T26T ( = CECT 8219T = IBRC-M 10759T = JCM 18750T).


2013 ◽  
Vol 2 (1) ◽  
pp. 150 ◽  
Author(s):  
Jamil Rima ◽  
Karine Assaker

<p>In this study, B-Cyclodextrinn polymerized with beetroot fibers (Bio-polymer), was prepared and applied to the removal of organic and inorganic contaminants from wastewater. An investigation into the use of cross-linked cyclodextrin polyurethanes copolymerised with beetroot fibers as adsorbents for organic pollutants and heavy metals has yielded very useful results which may have an impact in future water treatment applications.</p> The Biopolymer was tested in water contaminated by dyes, polycyclic aromatic hydrocarbons (PAH) and heavy metals. The effectiveness to eliminate dyes such as methylene blue and Rhodamine B with concentrations around 100 ppm was more than 99%, while the pyrene,which was chosen as an example among PAHs, showed a potential of elimination exceeding the 97% for solutions of 10 ppm. Also, heavy metals, such as Lead, Zn, and Cu, were tested and showed an efficacy exceeding the 99.8%. The results indicated that the biopolymer developed in this study has the potential to be a promising material for the removal of mixed pollutants from industrial wastewater or from contaminated groundwater.


Sign in / Sign up

Export Citation Format

Share Document