scholarly journals Intestinal mucosa development and blood profile of broiler chickens administered bovine testicular fluid

2021 ◽  
Vol 6 (3) ◽  
pp. 120-127
Author(s):  
E. O. Ewuola ◽  
◽  
A. T. Olujimi ◽  
M. O. Oyebanjo ◽  
T. F. Taiwo ◽  
...  

A seven-week experiment was carried out to determine the effect of bovine testicular fluid on intestinal mucosa development, haematological variables and serum biochemical indices of broiler chickens. A total of 125 unsexed day-old Abor acre chicks were randomly allotted into five treatments, with each treatment having five replicates with five birds per replicate. They were fed a commercial broiler diet for seven weeks of the experiment. Different concentrations of the bovine testicular fluid per liter of drinking water were offered to the experimental birds at free choice, the birds in treatment 1 served as the control and they were offered ordinary drinking water while birds in treatments 2, 3, 4 and 5 were offered 25, 50, 75 and 100 ml of bovine testicular fluid per liter of water, respectively. The feed and water intake of each bird were measured on daily basis, while the average weight of the birds was measured on weekly basis. On day 21 and 49, blood samples were collected from the birds to determine the haematological and serum biochemical indices. Two birds from each replicate were sacrificed on day 49 and sample of ileum were taken for intestinal mucosal examination after processed into permanent slides. The results of the blood indices obtained on days 21 and 49 indicated that the blood parameters were within the normal physiological range for chickens and without evidence of organ damage. The villus height, villus width, crypt depth, crypt height, and mucosa thickness showed that the bovine testicular fluid increased the absorptive capacity of the intestinal mucosa surface area and their growth. It is recommended that bovine testicular fluid at 100 ml/L improved intestinal mucosa development and absorption capacity of broiler chickens and enhanced their growth without deleterious effect on their health status.

2020 ◽  
Vol 45 (1) ◽  
pp. 272-278
Author(s):  
O. O. Lawrence-Azua ◽  
A. A. Saka ◽  
M. O. Yahaya ◽  
T. I. Ijadunola ◽  
A. M. Bernard ◽  
...  

Corn-Cob Meal (CCM) is a waste product derived from maize grain shelling corn cob which constitutes nuisance to the environment and it can be included in poultry diet in order to reduce competition between man and animal. Hence, a 63-day feeding trial was conducted in a completely randomized design to determine performance characteristics and blood profile of broiler chickens fed corn-cobs based diets with or without enzyme supplementation. One hundred and sixty five (165) birds were randomly allotted into five dietary treatment groups; T1: (0% CCM), T2 (10% CCM without enzyme), T3 (20% CCM without enzyme), T4 (10% CCM with enzyme), T5 (20% CCM with enzyme) with each treatment comprising three replicates of eleven birds per replicate. Parameters measured include performance characteristics, haematological and serum biochemical indices. The result revealed that there were no significant difference (P>0.05) on performance characteristics of broiler chicken observed except for Average Daily Feed Intake (ADFI) and the Feed Conversion Ratio (FCR). Birds fed 10% and 20% CCM based diet without enzyme recorded the highest value of ADFI (129.38 g/b/d and 130.85 g/b/d) while birds fed 20% CCM based diet with enzyme supplementation had the best FCR value (2.18). The haematological parameters were not significantly influenced (P>0.05) by the diets except for lymphocytes and heterophil count with the highest lymphocytes value in T2 (68.00%) and the lowest value in T5(53.67 %). Birds on T5 had significantly (P<0.05) highest heterophil value (40.67%). The serum biochemical indices were not significantly influenced (P>0.05) by the diets except for aspartate aminotransferase. However, values obtained in this study fell within the normal range recommended for healthy broiler chicken. Based on this result, it can be concluded that CCM with enzyme supplementation can be incorporated into broiler diet up to 20% without any detrimental effect on their performance and blood profile.  


Author(s):  
S. O. Omoikhoje ◽  
S. A. Eguaoje ◽  
J. Edonije ◽  
E. Osayande ◽  
J Edonije

An eight-week feeding trial was conducted to evaluate the effect of substituting maize with indomie waste meal on the haematology and serum biochemical indices of 90-dayold Anak 2000 broiler chickens. Three experimental broiler starter and finisher diets were formulated with diet 1 serving as control containing 0% Indomie waste meal (IWM), while diets 2 and 3 contained IWM at 50 and 100% inclusion levels for maize. The chicks were randomly assigned to the three treatment diets (1, 2 and 3) in a completely randomized design and each treatment group contained three replicates with ten chickens per replicate. The result on the haematological indices revealed that MCV values were significantly influenced (p<0.05) by the treatment diets with highest value of (135.50±0.21fl) among birds fed 50% IWM. MCH also significantly varied(p<0.05) among birds fed the treatment diets with highest (42.77±0.31pg) recorded among birds maintained on the control diet. Serum biochemical indices revealed that phosphorus value were significantly higher (p<0.05) in birds fed the control diet with the mean value of 3.63±0.09mg/100ml. Total cholesterol was significantly higher (p<0.05) in birds fed 100% IWM. The overall results from this study conclusively suggest that the inclusion of indomie waste meal in the diet of broilers up to 100% did not have any detrimental effect on the blood profile of the birds. But precaution had to be taken in the incorporation of IWM at this level because of the increase effect on the cholesterol level of the blood.Mr. Omoikhoje, B. (Laboratory Technologist)


2019 ◽  
Vol 17 (2) ◽  
pp. 81-94
Author(s):  
A. O. LALA ◽  
M. K. OLANREWAJU ◽  
A. P. OLANREWAJU ◽  
R. A. SANUSI ◽  
A. L. OLATUNDE ◽  
...  

Pre gelatinized cassava grit (PGCG) is a new cassava product produced mechanically and commercially for poultry feeding. Five dietary treatments were formulated with PGCG replacing maize at 0, 25, 50, 75 and 100 % in broiler starter (0-4 weeks) and finisher (4-8 weeks) diets. Two hundred (200) day-old broiler chickens were allotted to the five dietary treatments in a completely random design.  Each treatment was replicated four times with 10 birds per replicate and 40 birds per treatment. At the end of week 4 and 8 of the experiment, data were collected on growth performance, nutrient digestibility, haematological and serum biochemical indices. Final weight and weight gain were significantly (P < 0.05) highest in broilers fed 25 % PGCG diet, followed by those fed control diet. While, (P < 0.05) similar and lower values were obtained from broilers fed other PGCG diets. Feed intake decreased (P < 0.05) with PGCG in the diets at the starting and finishing phases. Dry matter and crude protein digestibility was (P < 0.05) highest in starting broilers fed 25 % PGCG diet, while digestibility (P < 0.05) declined with higher levels of PGCG. At the finishing phase, digestibility of all nutrients was similar (P < 0.05). Apparent metabolizable energy was (P < 0.05) higher in birds fed PGCG diets in the starting and finishing phases. Haematological and serum biochemical indices showed no significant (P>0.05) difference in the broiler chickens fed varying levels of PGCG in the diets. Broiler chickens fed PGCG above 25% in the diet had significantly (P < 0.05) higher proventriculus values when compared with those fed the control diet and 25% PGCG diet. The study revealed that substituting maize with 25 % PGCG in broiler diets improved growth and nutrient digestibility. Reduction in weight gain and non significant increased thiocyanate at higher PGCG inclusion should be improved for effective utilization of pre gelatinized cassava grit in broiler diets.  


2020 ◽  
Vol 44 (2) ◽  
pp. 101-111
Author(s):  
S. O. Omoikhoje ◽  
J. E. Imade ◽  
M. O. Odiase

An eight weeks feeding trial was conducted to assess the effect of feeding graded level of soaked pigeon pea meal (SPPM) in wood ash extract or solution on the growth performance, haematological and serum biochemical indices of broiler chickens. A total of 144 day-old chicks (Anak 2000) were randomly allotted to four treatment diets (1, 2, 3 and 4) in a completely randomized design. Each treatment group was replicated three times with twelve birds per replicate, thus, having thirty-six chicks per treatment group. Diet 1 contained 0% SPPM in wood ash extract for 72hours as the control diet, while diets 2, 3 and 4 were formulated to contain 25, 50 and 75% inclusions of SPPM in place of soybean meal. Results showed that the average live weight, daily weight gain and feed intake as well as protein efficiency ratio were significantly (P<0.05) influenced by the dietary treatments at the finisher phase. Broiler chickens fed 25% SPPM based diet had a higher (P<0.05) and comparable value of live weight, weight gain and protein efficiency ratio to that recorded in the control diet at the finisher phase. The haematological and serum biochemical indices revealed that albumin and total cholesterol were significantly (P<0.05) influenced by the treatment diets. The albumin value (2.2g/dl) was highest in birds fed 50% SPPM, whereas, the cholesterol value decreased (P<0.05) with increasing levels of inclusion of SPPM from 0 to 75%. It therefore implies that SPPM can be partially included in broiler diets up to 25% level with optimum growth performance and without any detrimental effect on the blood profile of broiler chickens.


2021 ◽  
Vol 44 (3) ◽  
Author(s):  
A. F Agboola

Since the cost of feed is increasing due to the keen competition between humans and animals, there is a need to critically seek for a matching alternative. Castor seed cake, a residue after the extraction of oil from nutrients-rich castor seed constitutes a waste which can be converted into livestock feed. Thus, the study was carried out to investigate the effect of graded levels of castor seed cake diets on haematological and serum biochemical indices of weanling albino rats in an experiment that lasted 21 days. Thirty weanling Wistar albino rats were weighed individually and randomly assigned to 5 dietary treatments with 3 replicates of two rats in a completely randomized design. Diet 1 was a nitrogen-free diet (NFD), while diets 2, 3, 4, and 5 had 0% (without castor seed cake inclusion), 2, 4 and 6% castor seed cake replacing soyabean meal respectively. At day 21, blood samples were obtained from two rats per replicate for haematological and serum analysis. There were no significant differences (P>0.05) observed in all the parameters (packed cell volume, haemaglobin, red blood cell, white blood cell, lymphocyte, neutrophil, monocyte, eosinophil and platelet) measured. Similarly, diet had no effect on the serum biochemical indices (creatinine, urea, total protein and globulin) measured. Significant difference (P<0.05) was however observed in albumin (2.62 – 3.18g/dL) of rats on the different experimental diets. The albumin concentrations of weanling albino rats on dietary treatments were similar to those on the control diet but significantly (P<0.05) higher (3.09 – 3.18g/dL) than those on the nitrogen free diet. It can be concluded that castor seed cake can replace soyabean meal up to 6% inclusion level without eliciting detrimental effect on blood profile of weanling wistar albino rats.


2020 ◽  
Vol 47 (4) ◽  
pp. 210-218
Author(s):  
A. O. Adekanbi ◽  
C. F. I. Onwuka ◽  
A. O. Oni ◽  
K. B. Jinadu ◽  
B. J. Adebayo ◽  
...  

Malted sorghum sprout (MSP) is an agro-industrial by-products rich in organic nitrogen and amino acids which can be used to enhance ruminant nutrition. A study was conducted to assess the nutritive value of MSP as supplementary on the performance characteristics and blood profile of West African dwarf (WAD) goats. MSP was collected, air-dried and then incorporated at different inclusion levels of 0, 10, 20 and 30% to formulate four dietary treatments T , T , T and T , respectively, with T - T being supplemented with Roxazyme G2 1 2 3 4 2 4 enzyme at 0.5g/kg. Twelve WAD bucks with an average weight of 7±0.5kg were assigned into four dietary treatments with three bucks per treatment in a completely randomized design over a period of 84 days. Data were obtained on performance characteristics, haematological and serum biochemical indices. Performance characteristics showed that average concentrate consumed (229.04g/day) and average daily weight gain (44.44 g/day) of WAD goats were significantly (P?0.05) higher in goats fed 20% MSP with enzyme supplementation. Dietary MSP with enzyme supplementation significantly (P?0.05) influenced the total protein (TP), aspartate amino transferase (AST), alanine amino transferase (ALT), white blood cells (WBC) and red blood cells (RBC) across the treatments. 3 3 TP (7.05g/dL) and WBC (7.88 x 10 /mm ) were significantly (P?0.05) higher in goats fed 20% MSP while globulin values increased significantly (P?0.05) across the dietary treatments (3.94 – 5.05g/dL) and is within the normal levels expected of healthy goats. It is concluded that dietary MSP with enzyme supplementation at 20% improved the performance, hematological and serum biochemical indicess of WAD goats without any adverse effects.


2020 ◽  
Vol 47 (3) ◽  
pp. 59-66
Author(s):  
H. O. Obikaonu ◽  
S. N. Obiora ◽  
P. Obiagwu

Attempts made to improve broiler production had been focused more on feed with little or no consideration on the impact of litter quality which is a major concern in chicken production not only because it affects the flock health and productivity, but also because it may act as potential reservoir and transmission vehicle for pathogens and potential pathogens. Since blood is considered as an important material for the evaluation of health status in animals, there is need therefore, to investigate the impact of different litter materials on the hematological and serum biochemistry of broiler finisher chicken. A56-day feeding trial was conducted to evaulate the effect of different litter materials on the haematological and serum biochemical indices of intensively managed finisher broilers. Four different litter materials were used for the experiment viz wood, shaving, Groundnut hulls; coconut husks and rice hulls. Wood shavings was used as the control owing to the fact that it is the most commonly used litter in this zone. The coconut husks were manually shred to extract the fibres which was used while the other materials were used as sourced. A toal of 120 broiler birds were used for the experiment, they were divided into four treatments (T1= Wood shavings, T2= Groundnut hulls, T3=Coconut husks, and T4=Rice hulls) of 30 birds each and sub divided into 10 birds per replicate. Each replicate was housed in a pen fitted with broding facilities. Feed and water were given ad libilum for eight weeks. At the end of the experiment blood was collected from the birds, three per treatment given a total of 12 birds. They were analyzed for haematological and serum biochemical indices. Haemoglobin values compared favourably (P>0.05) with that of the control. PCVvalues of T2 and T3 compared favourably well with that of control but significant differences existed (P<0.05) between the PCV value of T4 and the other treatments. The lymphocytes and neutrophils were not affected by the bedding materials showing that the litter conditions did not adversely affect the health and welfare of the birds. Serum protein, serum albumin and serum globulin were not affected by treatements (P>0.05) resulting in better protein efficiency ratio recorded in all the treatments. Serum electolytes calcium and phosphorus levels recorded in this study shows no adverse effect on the integrity of the liver in boosting cataion/anaion exchange. The haematological and serum biochemical indices recorded in this study showed no notable health challenges on the birds.


2020 ◽  
Vol 44 (1) ◽  
pp. 136-143
Author(s):  
E. O. Ewuola ◽  
A. A. Adeyemi ◽  
O. R. Bello

Variations in haematological and serum biochemical indices among White Fulani bulls, Ouda rams and West African Dwarf (WAD) bucks were studied. Ten each of mature bulls, rams and bucks were used for the experiment. Blood was sampled from all the animals and analysed for haematology and serum was separated for serum biochemical analysis. The result showed that species had effect on some haematological and serum biochemical indices of White Fulani bulls, Ouda rams and WAD bucks. Packed cell volume, haemoglobin, white blood cells, red blood cells, monocytes and eosinophils were not significantly different among the species, while platelets, lymphocytes, and neutrophils counts were significantly influenced by specie differences. Platelets was significantly (p<0.05) lower in rams (93.20×103 /µL) than in bulls (136.00×103 /µL) and bucks (141.90×103 /µL). Lymphocytes and neutrophils count was significantly (p<0.05) influenced by species differences. Serum biochemical parameters showed that there was no significant difference in the mean values for albumin, glucose, cholesterol, urea and Aspartate amino transferase, while the values obtained for total protein, creatinine and Alanine amino transferase (ALT) were significantly (p<0.05) different among the animal species. Total protein (7.21±0.52 g/dL) and creatinine (1.21±0.19 mg/dL) were significantly (p<0.05) higher in bucks than other species. The ALT was also higher (p<0.05) in bucks (20.83 iu/L) than in rams (7.59 iu/L) and bulls (6.27 iu/L). This study suggests that the effect of specie of an animal influences blood indices. The values obtained in this study can be used as references for haematological and serum biochemical assessment of indigenous male cattle, sheep and goat in Nigeria.


2015 ◽  
Vol 42 (2) ◽  
pp. 168-173
Author(s):  
O. E. Lawal ◽  
D. Eruvbetine ◽  
R. A. Sobayo ◽  
O. O. Olowofeso

The effect of phytobiotics inclusion on haematological and serum indices of broiler chickens was investigated using 180 one day-old Arbor-acre broiler chicks in an eight weeks feeding trial. Six experimental diets were formulated to include the control diets (Og/kg), Ginger (5g/kg), Pawpaw leaf (5g/kg), Moringa (5g/kg), Turmeric(1g/kg) and Oxytetracycline (0.5g/kg) diet. These diets were assigned to six groups of 30 chicks replicated 3 times with 10 birds per replicate in a completely randomized design (CRD). Feed and water were given ad libitum. At the end of eight weeks, blood samples were collected from jugular vein of the birds and were analyzed for haematological and serum biochemical indices. The result of the analysis showed that dietary treatments significantly (p<0.05) influenced all haematological parameters studied. Ginger had the highest value of packed cell volume (45%), haemoglobin (15.05 g/dl) and red blood cell count (3.39x1012L-1) at the finisher phase. Total protein, uric acid, glucose were significant (p<0.05). Serum cholesterol value was significantly (p<0.05) lower in birds supplemented with ginger compared to other groups. It can be concluded that ginger at 5g/kg improved PCV, Hb and reduction of cholesterol concentration in broiler chicken.


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